DNA methylation and mRNA expression of COL6A3 in antler mesenchyme of female and male reindeer

Jian‑Cheng Zhai,Ruo‑Bing Han,Sheng‑Nan Wang,Qiang‑Hui Wang,Yan‑Ling Xia,Wei‑Shi Liu,Ya‑Jie Yin,He‑Ping Li 한국유전학회 2019 Genes & Genomics Vol.41 No.9

Backgroud Reindeer is the only deer species that both male and female produce antlers, which provides a particularly interesting case in studying the differences between antlers of the two sexes. Alpha 3(VI) Collagen Gene (COL6A3), forms a microfibrillar network associated with the structural integrity and biomechanical properties, has been found to be one of the differentially expressed genes in antler mesenchyme of female and male reindeer. Objective and Methods The promoter sequence of reindeer COL6A3 gene was obtained using the cloning technology and analyzed by the bioinformatics methods. Bisulfite sequencing PCR (BSP) was used to detect the methylation status of the COL6A3 promoter in reindeer antler mesenchyme. Real-time quantitative PCR was used to detect COL6A3 expression in the antler mesenchyme of female and male reindeer. Results Sequence analysis revealed that the reindeer COL6A3 partial promoter sequence was 983 bp including the possible promoter region at + 105 bp to + 155 bp. Homology and phylogenetic analysis indicated that the COL6A3 promoter of reindeer had the closest genetic distance with Bos taurus, Capra hircus and Ovis aries. BSP results indicated that the methylation level of COL6A3 promoter in the female reindeer antler mesenchyme was significantly higher than in the male. Correlating with increased methylation status, we also found that COL6A3 mRNA expression in female reindeer antler mesenchyme was significantly lower than in the male. Conclusion The higher methylation level of the COL6A3 gene in female reindeer antler mesenchyme coincides with decreased COL6A3 mRNA expression, thereby affecting the transposon silencing mechanism and possibly contributing to apparent differences of antlers in female and male reindeer.

Distribution of Bi–MnS Inclusions Existing in 1215MS Steels: Correlation with Thermal Physical Coefficient

Jian‑bo Xie,Bei‑bei Liu,Xiang‑yu Wu,Jian‑xun Fu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.5

This work aims to comprehensively summarize the types and distribution of the isolated Bi particles and MnS inclusions inthe Bi-bearing steels, to study the precipitated behavior of sulfide, and to study the effect of Bi particles on grain sizes andthe volume thermal expansion coefficients of the steels. Results show that the average percentage of the isolated Bi particles,semi-encapsulation, complete encapsulation, back-bridge, tiny encapsulation, cavity bridge Bi–MnS inclusions in the Bibearingsteels was 16.89%, 20.49%, 3.44%, 16.89%, 31.63%, 10.66%, respectively. The average equivalent diameters of thegrains in the 0 ppm, 130 ppm, 240 ppm, 760 ppm, 1200 ppm and 2300 ppm Bi content steels were 20.88, 20.17, 19.54, 18.13,13.98 and 13.25 μm, respectively. The precipitated equilibrium mass concentration product of the MnS (w[Mn]·w[S]) wascalculated to be 1.07, and the solid fraction fS value was 0.61. At 950 °C, the volume thermal expansion coefficient change(ΔVE) in 760 ppm Bi content steel was minimum 4 × 10–10%, while at 461.6 °C the ΔVE was maximum, 0.09%, which meansthat Bi affected the volume thermal expansion of the steel strongest in this temperature range.

How “Foreign Influencer” Present Compelling Chinese Stories--Take “Chris”, a short video blogger in Tik Tok, as an example

Liu Jian(Jian Liu),Fei Jingying(Jingying Fei),Lv Mingkang(Mingkang Lv) 아시아사회과학학회 2022 International Science Research Vol.2 No.3

In recent years, a large number of excellent foreign short video bloggers have been active on major social media platforms, comparing the differences between Chinese and Western cultures, introducing Chinese traditional culture and customs to followers, and becoming a new force in telling Chinese stories. This paper takes the short video blogger “Chris” and his short videos as the research object, and analyze how “foreign influencer” tells Chinese stories in the new media environment, which brings new inspiration for telling Chinese stories and spreading Chinese voices well in the new media environment.

3 - D Seismic Images of Crust and Upper Mantle beneath Bohai Basin and its adjacent regions , Eastern China

(Jian Hua Liu),(Fu Tain Liu),(Jian Kun He),(Hui Chen) 대한지구물리학회 2000 지구물리 Vol.3 No.4

N/A N/A

Expression and Prognostic Implications of FOXO3a and Ki67 in Lung Adenocarcinomas

Liu, Hong-Bin,Gao, Xiang-Xiang,Zhang, Qing,Liu, Jian,Cui, Yuan,Zhu, Yan,Liu, Yi-Fei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

To investigate the significance of FOXO3a and Ki67 in human lung adenocarcinomas. Envision immunohistochemical staining and Western blotting were used to examine the protein expression of FOXO3a in 127 cases of human lung adenocarcinoma specimens. The positive rate in lung adenocarcinoma (55.9%) was lower than that in normal tissues (80%). We found that the expression of FOXO3a was closely related with the degree of differentiation, TNM staging, lymph node metastasis and survival. In addition, significant differences in the different pathological types of lung adenocarcinoma cases (P<0.01). The FOXO3a positive rate of the acini as the main type (APA) (86.7%) and the lepidic as the main type (LPA) (82.4%) was higher than the solid as the main type (SPA) (50.0%), the papilla as the main type (PPA) (42.9%) and the micropapilla as the main type (MPA) (9.4%). Moreover, the expression of FOXO3a was negatively related with Ki67 expression. Our results suggested that the expression of FOXO3a is closely correlated with the aggressiveness of lung adenocarcinoma. It was indicated that disregulation of FOXO3a might play key roles in the occurrence and development of lung a denocarcinoma and joint detection of the two markers might play an important role in diagnosing tumors.

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu, Wei-Jun,Ren, Jian-Guo,Li, Ting,Yu, Guo-Zheng,Zhang, Jin,Li, Chang-Sheng,Liu, Zhi-Su,Liu, Quan-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

Comparative Study of Contrast-Enhanced Ultrasound Qualitative and Quantitative Analysis for Identifying Benign and Malignant Breast Tumor Lumps

Liu, Jian,Gao, Yun-Hua,Li, Ding-Dong,Gao, Yan-Chun,Hou, Ling-Mi,Xie, Ting Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: To compare the value of contrast-enhanced ultrasound (CEUS) qualitative and quantitative analysis in the identification of breast tumor lumps. Materials and Methods: Qualitative and quantitative indicators of CEUS for 73 cases of breast tumor lumps were retrospectively analyzed by univariate and multivariate approaches. Logistic regression was applied and ROC curves were drawn for evaluation and comparison. Results: The CEUS qualitative indicator-generated regression equation contained three indicators, namely enhanced homogeneity, diameter line expansion and peak intensity grading, which demonstrated prediction accuracy for benign and malignant breast tumor lumps of 91.8%; the quantitative indicator-generated regression equation only contained one indicator, namely the relative peak intensity, and its prediction accuracy was 61.5%. The corresponding areas under the ROC curve for qualitative and quantitative analyses were 91.3% and 75.7%, respectively, which exhibited a statistically significant difference by the Z test (P<0.05). Conclusions: The ability of CEUS qualitative analysis to identify breast tumor lumps is better than with quantitative analysis.

Eliminating the Third Harmonic Effect for Six Phase Permanent Magnet Synchronous Generators in One Phase Open Mode

Liu, Jian,Yang, Gui-Jie,Li, Yong,Gao, Hong-Wei,Su, Jian-Yong The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1

To insure stable operation and eliminate twice torque ripple, a topology for a six phase permanent magnet synchronous generator (SP-PMSG) with a neutral point connected together was analyzed in this paper. By adopting an extended transformation matrix, the mathematic model of the space vector control was established. The voltage and torque equations were deduced while considering the third harmonic flux and inductance. In addition, the suppression third harmonic method and the closed loop control strategy were proposed. A comparison analysis indicates that the cooper loss minimum method and the current magnitude minimum method can meet different application requirements. The voltage compensation amount for each of the methods was deduced which also takes into account the third harmonic effect. A simulation and experimental result comparison validates the consistency through theoretical derivation. It can be seen that all of the two control strategies can meet the requirements of post-fault.

The International Communication of the “Belt and Road Initiative” Major Projects: Take the “China-Laos Railway” as an Example

Jian Liu,Yiran Wu,Benqi Yin 아시아사회과학학회 2023 Jornal of Asia Social Science Vol.10 No.3

Through the international communication of “China-Laos Railway”, a landmark project of the “Belt and Road Initiative”, China showcased to the world that the initiative represents a “Golden Path” for regional economic development. It is a path that drives progress for the involved countries through Chinese path to modernization and a route for peaceful development in the new era. Analyzing the international communication experience of the “China-Laos Railway” and existing issues, and proposing solutions can offer valuable insights for current and future international communications, which will contribute to garnering more international support for China’s “Belt and Road Initiative” and facilitate the smooth implementation of related projects.

Development and application of high-performance compound salt drilling fluid

Jian Wang,Hanxuan Song,Jian Guan,Hongyu Liu 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.5

Due to the characteristics of deep burial, abnormal pressure, high bottom hole temperature, and abundant fractures, the tightoil reservoirs in the Leijia block of Liaohe Oilfield have many complex situations such as lost circulation and electricalresistance encountered, which seriously restricts the development of tight oil and gas resources in Leijia. Safe and efficientdevelopment. To solve this problem, firstly, the mechanism of the wellbore instability of the tight mud shale in the Leijia blockwas studied. Then, the formula of the composite salt base fluid was optimized by using the principle of activity balance, andthe nano-plugging with core-shell structure was developed in a targeted manner. Finally, a high-performance drilling fluidsystem with strong plugging, strong inhibition and strong lubrication was constructed. The temperature resistance of thesystem reaches 150 °C, the primary recovery rate of shale is 97.8%, the permeability is reduced by more than 50%, and HTHP(150 °C) is less than or equal to 8 mL. In the test of Well L77-HX in Leijia block, the accident complexity rate is 0, the successrate of electrical measurement is 100%, the ROP is increased by 40%, the drilling depth is 5000m, and the horizontaldisplacement is 1783m. This new compound salt high-performance drilling fluid system provides technical support.