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      • KCI등재

        Early gonadal maturation and vitellogenin mRNA expression in Siberian sturgeon Acipenser baerii cultured in a semi-closed water recirculating system in Korea

        Chulhong Park,Seung Pyo Gong,Youn Hee Choi,Ki Hong Kim,Yoon Kwon Nam 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.3

        Changes of gonadal morphology and mRNA expression patterns of vitellogenin were investigated in Siberian sturgeon Acipenser baerii (Chondrostei) during its early gonadal maturation period. Early differentiations and morphological transitions of both ovaries and testes appeared to occur actively until the age of 3 years, however from then on, the maturation patterns to full maturity were largely gender-dependent, in which males showed a faster progression of maturation than did females while females experienced a steady-state progress with a lagged interval before entering the final maturation. Expression of vitellogenin mRNAs are closely correlated with transitional patterns of gonadal appearances. In both females and males, hepatic mRNA levels of vitellogenin exponentially increased in the earliest interval (up to 1-year-old). However, in subsequent periods, vitellogenin expression in females continued to increase with age, whereas in males, the expression stabilized at a younger age. Nevertheless, at the age older than or equal to 7-year-old, fully matured individuals showed a quite low level of vitellogenin expression in both females and males. Collectively, results from this study could be useful as a fundamental guideline to address the gonad maturation of this sturgeon species, which is helpful for making practical decisions about farming practices and management for caviar production on local sturgeon farms.

      • KCI등재

        Cyclic variations of gonad development of an air-breathing fish, Channa striata in the lentic and lotic environments

        Nasim Al Mahmud,Hassan Md. Hafizur Rahman,Golam Mohammod Mostakim,Mohd. Golam Quader Khan,Md. Shahjahan,Nahid Sultana Lucky,M. Sadiqul Islam 한국수산과학회 2016 Fisheries and Aquatic Sciences Vol.19 No.1

        The study was conducted to know the cyclic changes in gonadal maturation and to investigate the developmental stages of oocytes and testicular germ cells of an air-breathing fish, Channa striata. Fish were sampled monthly from lentic and lotic environments of three geographical locations of Bangladesh from December to November and the histological analysis of their gonad was done to evaluate the objectives. The highest mean GSI was 5.95 ± 0.20 for female in July and 0.14 ± 0.01 for male also in July showing that the gonadal development reached its peak during this month. The highest mean oocyte diameter was 1257.50 ± 24.17 μm observed in July implying that the oocyte reached maturity in this month. Histological study of ovary revealed the evidence of early yolk granule stage and late yolk granule stage from April to July. In case of male four stages of spermatogenesis were distinguished and spermatozoa were highly abundant in June and July. So the monthly pooled values of GSI and the analysis of gonadal histology indicated that the peak breeding season of C. striata occurred in July in the lentic and lotic environments. Samples collected from lentic and lotic habitats are suggestive of no difference in the development of the gonad. The results of the present study will be useful for selective breeding programme, conservation and sustainable fishery management of C. striata in its natural habitat.

      • KCI등재

        Early gonadal maturation and vitellogenin mRNA expression in Siberian sturgeon Acipenser baerii cultured in a semi-closed water recirculating system in Korea

        Park, Chulhong,Gong, Seung Pyo,Choi, Youn Hee,Kim, Ki Hong,Nam, Yoon Kwon The Korean Society of Animal Reproduction and Biot 2020 한국동물생명공학회지 Vol.35 No.3

        Changes of gonadal morphology and mRNA expression patterns of vitellogenin were investigated in Siberian sturgeon Acipenser baerii (Chondrostei) during its early gonadal maturation period. Early differentiations and morphological transitions of both ovaries and testes appeared to occur actively until the age of 3 years, however from then on, the maturation patterns to full maturity were largely gender-dependent, in which males showed a faster progression of maturation than did females while females experienced a steady-state progress with a lagged interval before entering the final maturation. Expression of vitellogenin mRNAs are closely correlated with transitional patterns of gonadal appearances. In both females and males, hepatic mRNA levels of vitellogenin exponentially increased in the earliest interval (up to 1-year-old). However, in subsequent periods, vitellogenin expression in females continued to increase with age, whereas in males, the expression stabilized at a younger age. Nevertheless, at the age older than or equal to 7-year-old, fully matured individuals showed a quite low level of vitellogenin expression in both females and males. Collectively, results from this study could be useful as a fundamental guideline to address the gonad maturation of this sturgeon species, which is helpful for making practical decisions about farming practices and management for caviar production on local sturgeon farms.

      • SCOPUSKCI등재

        Sexual Maturation, Sex Ratio and Hermaphroditism of the Pacific Oyster, Crassostrea gigas, on the West Coast of Korea

        Chung Ee-Yung,Seo Young-Ho,Park Kwan Ha The Korean Society of Fisheries and Aquatic Scienc 1998 Fisheries and Aquatic Sciences Vol.1 No.1

        Monthly changes of the gonad follicle index (GFI), reproductive cycle, egg-diameter composition, first sexual maturity of the Pacific oyster, Crassostrea gigas, were studied based on the samples which have been collected from the intertidal zone of Poryong west coast of Korea, from January to December, 1996. C. gigas, is dioecious, while a few individuals are alternatively hermaphroditic. Monthly variation of gonad follicle index (GFI) used for determination of spawning period, coincided with the reproductive cycle. GFI increased from April when seawater temperatures gradually increased and reached the maximum in May. And then, GFI sharply decreased from June to September due to spawning. Reproductive cycle of this species can be divided into five successive stages: in females, early active stage (March to April), late active stage (April to May), ripe stage (May to August), partially spawned stage (June to September) and spent/inactive stage (September to February); in males, early active stage (February to March), late active stage (April to May), ripe stage (May to September), partially spawned stage (June to September) and spent/ inactive stage (September to February). The diameter of fully mature eggs are approximately 50um. Spawning occurred from June to September, and two spawning peaks were observed in June and August when the seawater temperature was above $20^{\circ}C$. Percentages of the first sexual maturity of males of 20.1-25.0 mm in shell height were over $50\%$, while those of females of 25.1-30.0 mm in shell height were over $50\%$. All the males of > 30.1 mm and all the females of ^gt; 35.1 mm completed their first sexual maturity. The results suggest that C. gigas has a protandry phenomenon. Sex ratios of 919 oysters observed were 453 females $(49.29\%)$, 429 males $(46.68\%)$, 16 hermaphrodites $(1.74\%)$, and 21 indeterminate individuals $(2.29\%)$. In age class I, sex ratio of males were $64.00\%$, thus, a higher percentage than that of females. It was noted that $64.00\%$ of the young males (age class I) were more functional than females in age class I, but 2-3 year-old oysters showed higher percentage of females. Percentages of hemaphrodites in 2-3 year classes were relatively higher than those in other year classes. Histological pattern of hermaphrodites can be divided into two types: Type I (hermaphrodite having a number of newly formed developing oocytes on the oogenic tissues within a degenerating spermatogenic follicle after discharge of numerous spermatozoa) and Type II (hermaphrodite having two separate follicles in the same gonad).

      • KCI등재후보

        Expression of Maturation-Related Genes and Leptin during Sexual Maturation in the Female Goldfish: Effects of Exogenous Kisspeptin

        Kim, Na Na,Choi, Young Jae,Oh, Sung-Yong,Choi, Cheol Young The Korean Society of Marine Life Science 2016 한국해양생명과학회지 Vol.1 No.1

        Kisspeptin (Kiss) and its cognate receptor, kisspeptin receptor (KissR; G protein coupled receptor 54, GPR54), have recently been recognized as potent regulators of reproduction in teleosts. Additionally, leptin plays an important role in energy homeostasis and reproductive function in teleosts. The purpose of this study was to examine differences in the concentration of the hormones of the Kiss/KissR system and leptin and the expression of their underlying genes, all of which are involved in the sexual maturation of female goldfish, Carassius auratus, following treatment with Kiss. The expression levels of KissR increased after the Kiss injection. Furthermore, the peptide hormone leptin also increased after the injection (in vivo and in vitro). Additionally, the expression of GnRH and GTHs (GTHα, FSHβ, and LHβ) increased in the brain and pituitary (in vitro and in vitro). These results support the hypothesis that Kiss plays important roles in the direct regulation of the hypothalamus-pituitary-gonad axis and leptin in goldfish. Therefore, we suggest that Kiss system gene expression is correlated with energy balance and reproduction.

      • Gonadal Development and Reproductive Cycle of the Top Shell, Omphalius rusticus(Gastropoda: Trochidae)

        Lee, Ju Ha The Korean Society for Integrative Biology 2001 Korean journal of biological sciences Vol.5 No.1

        Gonadal development, reproductive cycle, gonad index, meat weight rate, and first sexual maturity of the top shell, Omphalius rusticus were Investigated monthly by histological observations. Specimens were collected from the west coast of Korea during the period from January to December in 1999. O. rusticus is dioecious and oviparous. The gonad is widely situated on the surface of the digestive g1and located in the posterior spiral meat part in the shell. The ovary and the testis were composed of a number of oogenic follicles and several spermatogenic follicles, respectively. Ripe oocytes were approximately 120-130 $\mu$m in diameter. The meat weight rate peaked in June (27.7%), and then rapidly decreased in September (19.5%). Monthly changes in the gonad index in both sexes reached the maximum in June, and then sharply decreased in September. Percentages of first sexual maturity of female and male snails ranging from 9.0 to 9.9 mm in shell heights were 58.3% and 54.5%, respectively, and 100% in those over 11.0 mm in both sexes participated in reproduction. Reproductive cycle of this species can be categorized into five successive stages: in females, early active (October to April), late active (December to June), ripe (April to September), spawning (July to September) and recovery (September to January): in males, early active (November to March), late active (December to June), ripe (April to September), spawning (July to September) and recovery (September to December). Gonadal development, gametogenesis, reproductive cycle, and spawning were closely related to the seawater temperature.

      • SCOPUSKCI등재

        Gonadal Maturation and Artificial Spawning of the Manila Clam, Ruditapes philippinarum (Bivalvia: Veneridae), in Komso Bay, Korea

        Chung Ee-Yung,Hur Sung Bum,Hur Young-Baek,Lee Jung Sick The Korean Society of Fisheries and Aquatic Scienc 2001 Fisheries and Aquatic Sciences Vol.4 No.4

        We have investigated the gonad index (GI), gonadal development, reproductive cycle, first sexual maturity, sex ratio, the number of spawned eggs and spawning frequency of the Manila clam, Ruditapes philippinarum. Samples were collected from the intertidal zone of Komso Bay, Korea from January to December in 1999. Monthly changes in the gonad index (GI) and condition index showed a similar pattern in the reproductive cycle. The spawning period was once a year between early June and early October, there was a spawning peak between July and August when seawater temperature was over $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (June to October), and spent/inactive stage (August to March). Percentages of first sexual maturity of female and male clams of l5.1-20.0mm in shell length were $56.3\%$ and $60.0\%$, respectively, and $100\%$ for the clams >25. mm. The sex ratio of individuals >15.1 mm in shell length was about 1:1 $(\chi^2= 0.02,\;p>0.05)$. Number of the eggs released from each clam by the induction increased as the size of clam in terms of shell length increased. Mean number of the eggs from the second induction of the spawning was $75.35-84.30\%$ $(average\;79.81\%)$ of the number of the eggs released in the first spawning. Our data indicated that R. philippinarum in Komso Bay has one major spawning peak with over two minor spawning, and the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.

      • KCI등재

        눈알고둥 (Lunella coronata coreensis)의 성 성숙

        이주하,LEE Ju Ha 한국수산과학회 2000 한국수산과학회지 Vol.33 No.6

        1998년 7월부터 1999년 6월까지 전라북도 부안군 대항리 앞바다의 조간대에서 매월 채집한 눈알고둥, Lunella coronsta coreenisis의 생식소발달, 생식세포형 성, 생식주기, 생식소지수, 육중량비 및 군성숙도를 조직학적으로 조사하였다. 눈알고둥은 자웅이체였으며, 생식소는 패각 내의 후반 나선부에 위치하는 간장의 표면에 넓게 분포하였다. 난소와 정소는 각각 수많은 난자형성소엽과 정자형성소낭으로 구성되어 있었다. 생식소지수의 월별 변화는 수온이 상승하는 3월 ($23.86{\pm}3.73$)부터 서서히 증가하여 7월 ($49.76{\pm}6.47$)에 최고치를 나타낸 후, 9월($15.58{\pm}2.33$)에 급격하게 감소하였다. 육중량비의 월별 변화는 $25.2{\%}{\~}32.3{\%}$로, 생식소지수의 월별 변화와 유사한 양상을 나타내었다. 각고 $5.9 mm$ 이하의 개체군은 재생산에 전혀 참여하지 않았으며, $7.0{\~}7.9 mm$의 개체군은 암수 각각 $84.6{\%}$ 와 $91.7{\%}$가 재생산에 참여하였고, 8.0 mm 이상의 개체군은 $100{\%}$ 참여하였다. 생식소의 발달, 생식세포형성과정, 조직분화과정 및 세포학적 특성에 따라, 생식주기는 초기활성기 (12월${\~}$4월), 후기활성기 (1월${\~}$7월), 완숙기 (5월${\~}$8월), 산란기 (7월${\~}$9월) 및 회복기 (9월${\~}$3월)등의 연속적인 5단계로 구분되었다. 산란은 수온이 $24.8^{\circ}C$ 이상인 7월과 9월 사이에 일어나며, 주산란은 8월이었고 성숙란의 직경은 $150{\~}160 {\mu}m$이었다. Gonadal development, gametogenesis, reproductive cycle, gonad index, flesh weight rate, and first sexual maturity of the turban shell, Lunella coronata coreensis were investigated by histological observation. The materials used were collected monthly from the rocky intertidal zone of Daehang-ri, Buan-gun, Jeollabuk-do, on the west coast of Korea, from July 1998 to June 1999. Sex of L coronata coreensis was separate. The gonad was widely located in the spirals of the visceral mass buried in the digestive gland. The ovary and testis were composed of a number of oogenic follicles and speymatogenic follicles, respectively. Monthly variations in the gonad index increased from March ($23.86{\pm}3.73$) when the water temperature increased and reached the maximun in July ($49.76{\pm}6.47$). And then, the gonad index sharply decreased in September ($15.58{\pm}2.33$). The flesh weight rate ranged from $25.2{\%}$ to $32.3{\%}$, and its variation showed a similar pattern to the gonad index. Individuals $<5.9 mm$ in shell height could not take part in reproduction in both sexes. Percentages of first sexual maturity of female and male specimens ranging from $7.0{\~}7.9 mm$ in shell heights were $84.6{\%}\;and\;91.7{\%}$, respectively, and $100{\%}$ in those over 8.0 mm in shell height in both sexes took part in reproduction. By studying the monthly changes of the morphological features and sizes of germ cells during gametogenesis in the gonad, the reproductive cycle of this species could be devided into five successive stages: early active (December to April), late active (January to July), ripe (May to August), spawning (July to September), and recovery (September to March). The spawning period of this species was once a year between July and September, and the main spawning occurred in July when the seawater temperature reached above $24.8^{\circ}C$. The fully ripe eggs were $150{\~}160\;{\mu}m$ in diameter.

      • KCI등재

        자귀나무 추출물이 송사리(Oryzias latipes) 생식소 성숙에 대한 억제효과

        이은희(Eun-Hee Lee),오상필(Sang-Pil Oh),김명희(Myong-Hee Kim),김광현(Kwang-Hyun Kim),홍상훈(Sang-Hoon Hong),한창희(Chang-Hee Han) 한국생명과학회 2013 생명과학회지 Vol.23 No.3

        사료에 첨가된 saponin이 함유된 자귀나무(Albizzia julibrissin) 껍질 추출물이 송사리(Oryzias latipes)의 생식소 성숙과 산란에 미치는 영향을 조사하기 위하여 자귀나무(A. julibrissin) 껍질의 n-BuOH 추출물로부터 Diaion HP-20, Silica gel과 Sephadex LH-20 chromatography들을 이용하여 조 사포닌 분획물(HaBC)을 분리하였다. 실험 어류들은 순환여과 장치 시스템의 수조에서 사육하였으며, HaBC를 첨가한 사료를 급여하여 암컷의 생식소 성숙 억제 및 산란 억제 효과를 조사하였다. 미성숙 송사리들에 대한 실험에서 사료에 HaBC를 20 mg/g-feed 이상 첨가한 사료를 먹인 어류들은 생식소의 성숙과 산란을 개시하는 시기가 지연되었다. 또한 성숙한 암컷 송사리들도 HaBC를 20 mg/g-feed 이상 첨가한 사료를 먹었을 때 대조구에 비해 낮은 GSI 값을 보였다. 미성숙한 송사리에 대한 길이 성장이나 체중에 대한 변화는 HaBC의 첨가량에 관계가 없었으나, 비만도(CF)에서는 HaBC를 첨가한 사료를 먹인 어류들이 대조구에 비해 높은 값을 보였다. 이러한 결과로 자귀나무(A. julibrissin)의 껍질로부터 분리한 saponin 분획물은 송사리 암컷의 성숙을 억제할 수 있었지만, 성장 촉진에는 아무런 작용을 하지 않았다. 이들 작용 기전에 대해서는 더 많은 연구들이 있어야 할 것이다. This study was performed to determine whether stem bark extract containing saponin of Albizzia julibrissin in the diet influences gonadal maturation and spawning in medaka (Oryzias latipes). The crude extraction containing saponin (HaBC) was partially purified from n-BuOH extraction of A. julibrissin stem bark by Diaion HP-20, Silica gel and Sephadex LH-20 chromatographies. We fed diets supplemented with HaBC to medaka. We then studied the effects of the HaBC supplement on the suppression of gonadal maturation and spawning in female medaka that were reared in aquaria with recirculation systems. In the experiment with immature female medaka, the periods of initiation of gonadal maturation and spawning were delayed in the fish that were fed diets supplemented with at least HaBC 20 mg/g-feed. In the experiment with mature female medaka, the fish that were fed diets supplemented with at least HaBC 20 mg/g-feed had lower GSIs than the control diet group did. The results showed that the growth of the immature medaka was not correlated with the amount of supplementation of HaBC in the diet. However, the condition factors (CF) in the medaka that were fed diets supplemented with at least HaBC 20 mg/g-feed were higher than in the medaka fed on the control diet. We concluded that extracts containing saponin from the stem bark of A. julibrissin have the potential to inhibit gonadal maturation in female medaka, but they did not act as growth stimulation. Further studies are required to determine the mechanism of the action.

      • KCI등재

        Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

        강희웅,조재관,손맹현,박종연,홍창기,Jae Seung Chung,정애영 한국발생생물학회 2015 발생과 생식 Vol.19 No.1

        The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

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