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      • SCIESCOPUSKCI등재

        Identification of Histone Deacetylase 2 as a Functional Gene for Skeletal Muscle Development in Chickens

        Shahjahan, Md.,Liu, Ranran,Zhao, Guiping,Wang, Fangjie,Zheng, Maiqing,Zhang, Jingjing,Song, Jiao,Wen, Jie Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.4

        A previous genome-wide association study (GWAS) exposed histone deacetylase 2 (HDAC2) as a possible candidate gene for breast muscle weight in chickens. The present research has examined the possible role of HDAC2 in skeletal muscle development in chickens. Gene expression was measured by quantitative polymerase chain reaction in breast and thigh muscles during both embryonic (four ages) and post-hatch (five ages) development and in cultures of primary myoblasts during both proliferation and differentiation. The expression of HDAC2 increased significantly across embryonic days (ED) in breast (ED 14, 16, 18, and 21) and thigh (ED 14 and 18, and ED 14 and 21) muscles suggesting that it possibly plays a role in myoblast hyperplasia in both breast and thigh muscles. Transcript abundance of HDAC2 identified significantly higher in fast growing muscle than slow growing in chickens at d 90 of age. Expression of HDAC2 during myoblast proliferation in vitro declined between 24 h and 48 h when expression of the marker gene paired box 7 (PAX7) increased and cell numbers increased throughout 72 h of culture. During induced differentiation of myoblasts to myotubes, the abundance of HDAC2 and the marker gene myogenic differentiation 1 (MYOD1), both increased significantly. Taken together, it is suggested that HDAC2 is most likely involved in a suppressive fashion in myoblast proliferation and may play a positive role in myoblast differentiation. The present results confirm the suggestion that HDAC2 is a functional gene for pre-hatch and post-hatch (fast growing muscle) development of chicken skeletal muscle.

      • KCI등재

        Graphene Oxide-Hydrogen Membrane Fuel Cell

        Md Shahjahan Kabir Chowdury,박성범,박용일 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.3

        Proton exchange membrane fuel cell (PEMFC) utilizes polymer electrolyte membrane, e.g., Nafi on™, which is a perfl uorosulfonic acid (PFSA) membrane with many disadvantages such as expensive, low mechanical strength, low chemical stability at high temperatures. Meanwhile, graphene oxide membrane (GOM) is an excellent proton conductor at room temperature under humidifi ed conditions. However, graphene oxide membrane fuel cells (GOMFCs) exhibit low maximum power density compared to Nafi on due to fuel gas crossover, membrane degradation and loss of surface functionalities. In this study, bilayer membrane consisting of GOM and hydrogen permeable metal thin fi lm was investigated as electrolyte membrane for hydrogen membrane fuel cell (HMFC). In this fuel cell, the graphene oxide-hydrogen membrane (GOHM) simultaneously plays two roles, an anode catalyst and an electrolyte. A hydrogen permeable metal thin fi lm of about 40 nm was deposited by DC magnetron sputtering at a suitable pressure and deposition time on a 12 μm-thick GOM using a Pd or Ni 64 Zr 36 target to complete the bilayer GOHM electrolyte. The fuel cell performance of the GOHMFC using with Pt-free anode was compared with the GOHMFC with conventional Pt/C electrodes and conventional PEMFC.

      • KCI등재

        Cyclic variations of gonad development of an air-breathing fish, Channa striata in the lentic and lotic environments

        Nasim Al Mahmud,Hassan Md. Hafizur Rahman,Golam Mohammod Mostakim,Mohd. Golam Quader Khan,Md. Shahjahan,Nahid Sultana Lucky,M. Sadiqul Islam 한국수산과학회 2016 Fisheries and Aquatic Sciences Vol.19 No.1

        The study was conducted to know the cyclic changes in gonadal maturation and to investigate the developmental stages of oocytes and testicular germ cells of an air-breathing fish, Channa striata. Fish were sampled monthly from lentic and lotic environments of three geographical locations of Bangladesh from December to November and the histological analysis of their gonad was done to evaluate the objectives. The highest mean GSI was 5.95 ± 0.20 for female in July and 0.14 ± 0.01 for male also in July showing that the gonadal development reached its peak during this month. The highest mean oocyte diameter was 1257.50 ± 24.17 μm observed in July implying that the oocyte reached maturity in this month. Histological study of ovary revealed the evidence of early yolk granule stage and late yolk granule stage from April to July. In case of male four stages of spermatogenesis were distinguished and spermatozoa were highly abundant in June and July. So the monthly pooled values of GSI and the analysis of gonadal histology indicated that the peak breeding season of C. striata occurred in July in the lentic and lotic environments. Samples collected from lentic and lotic habitats are suggestive of no difference in the development of the gonad. The results of the present study will be useful for selective breeding programme, conservation and sustainable fishery management of C. striata in its natural habitat.

      • SCIESCOPUSKCI등재

        Nucleation of Diamond over Nanotube Coated Si Substrate Using Hot Filament Chemical Vapor Deposition (CVD) System

        Ansari, Shafeeque G.,Seo, Hyung-Kee,Kim, Gil-Sung,Shahjahan, Md.,Shin, Hyung-Shik 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1

        We studied the use of carbon nanotubes as a seeding layer for the nucleation of diamond on Si (100) substrate by using a hot filament chemical vapor deposition (HFCVD) system. Prior to deposition, substrates were seeded with multi-wall carbon nanotube (MWCNT) powder which was prepared separately. MWCNTs were used as nucleation precursors. The diamond grains grew essentially over the nanotubes with a higher growth density in comparison with the un-seeded substrates. The scanning electron microscopy (SEM) image of surface morphology shows crystallites of cauliflower shaped grains. The micro Raman spectroscopic results show a sharp peak at 1,332㎝^(-1) corresponding to diamond phase, X-ray photoelectron spectroscopic study show the presence of carbon (C_(1x)) phase.

      • KCI등재

        Nucleation of Diamond over Nanotube Coated Si Substrate Using Hot Filament Chemical Vapor Deposition (CVD) System

        신형식,김길성,Shafeeque G. Ansari,Hyung-Kee Seo,Mushtaq Ahmad Dar,Md. Shahjahan 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1

        We studied the use of carbon nanotubes as a seeding layer for the nucleation of diamond on Si (10) sub-strate by using a hot filament chemical vapor deposition (HFCVD) system. Prior to deposition, substrates were seededwith multi-wal carbon nanotube (MWCNT) powder which was prepared separately. MWCNTs were used as nucle-ation precursors. The diamond grains grew esentialy over the nanotubes with a higher growth density in comparisonwith the un-seeded substrates. The scaning electron microscopy (SEM) image of surface morphology shows crystal-lites of cauliflower shaped grains. The micro Raman spectroscopic results show a sharp peak at 1,332 cm-1 corespond-ing to diamond phase. X-ray photoelectron spectroscopic study show the presence of carbon (C1s) phase.

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