Effect of gamma irradiation on Korean traditional multicolored paintwork

Yoon, Minchul,Kim, Dae-Woon,Choi, Jong-il,Chung, Yong-Jae,Kang, Dai-Ill,Hoon Kim, Gwang,Son, Kwang-Tae,Park, Hae-Jun,Lee, Ju-Woon Elsevier 2015 Radiation physics and chemistry Vol.115 No.-

<P><B>Abstract</B></P> <P>Gamma irradiation can destroy fungi and insects involved in the bio-deterioration of organic cultural heritages. However, this irradiation procedure can alter optical and structural properties of historical pigments used in wooden cultural heritage paintings. The crystal structure and color centers of these paintings must be maintained after application of the irradiation procedure. In this study, we investigated the effects of gamma irradiation on Korean traditional multicolored paintwork (Dancheong) for the preservation of wooden cultural heritages. The main pigments in Korean traditional wooden cultural heritages, <I>Sukganju</I> (Hematite; Fe<SUB>2</SUB>O<SUB>3</SUB>), <I>Jangdan</I> (Minium; Pb<SUB>3</SUB>O<SUB>4</SUB>), <I>Whangyun</I> (Crocoite; PbCrO<SUB>4</SUB>), and <I>Jidang</I> (Rutile; TiO<SUB>2</SUB>), were irradiated by gamma radiation at doses of 1, 5, and 20kGy. After irradiation, changes in Commision Internationale d’Eclairage (CIE) color values (<I>L</I>*, <I>a</I>*, <I>b</I>*) were measured using the color difference meter, and their structural changes were analyzed using X-ray diffraction (XRD) analysis. The slightly change in less than 1 <I>dE</I>* unit by gamma irradiation was observed, and structural changes in the Dancheong were stable after exposure to 20kGy gamma irradiation. In addition, gamma irradiation could be applied to painted wooden cultural properties from the Korean Temple. Based on the color values, gamma irradiation of 20kGy did not affect the Dancheong and stability was maintained for five months. In addition, the fungicidal and insecticidal effect by less than 5kGy gamma irradiation was conformed. Therefore, the optical and structural properties of Dancheong were maintained after gamma irradiation, which suggested that gamma irradiation can be used for the preservation of wooden cultural heritages painted with Dancheong.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of gamma irradiation on the Dancheong were evaluated. </LI> <LI> We confirmed that optical and structural properties of Dancheong were maintained. </LI> <LI> Irradiation can contribute the decontamination for wooden cultural heritages. </LI> <LI> It also can be used for preservation of painted-wooden cultural heritages. </LI> </UL> </P>

Comparison of the efficacy of gamma and UV irradiation in sanitization of fresh carrot juice

Jo, Cheorun,Lee, Kyung Haeng Elsevier 2012 Radiation physics and chemistry Vol.81 No.8

<P><B>Abstract</B></P><P>As there is no pasteurization procedure for the manufacture of fresh vegetable juice, both industry and consumers have sought a method for improving the storage stability and shelf-life of this category of products. In this study, the effects of commercially available, non-thermal pasteurization processes, such as gamma and UV irradiation, were compared for their efficacy in sanitizing fresh carrot juice (FCJ). FCJ was manufactured, packaged, and gamma irradiated with doses of 0, 1, 3, and 5kGy. The manufactured FCJ was also passed through 4 UV light lamps at doses of 3.67, 4.69, and 6.50kGy. The total aerobic bacterial count of the FCJ approached the legal limit (10<SUP>5</SUP> CFU/mL) after manufacturing. Both treatments were effective in reducing the number of total aerobic bacteria, and the reduced number was maintained during storage for 7 days. Gamma irradiation was more effective in suppressing microbial growth during storage. When the doses for UV treatment and gamma irradiation were higher, the inactivation effects were higher. The reduction of ascorbic acid content was greater upon gamma irradiation than UV treatment. No difference was found in the contents of flavonoids and polyphenols in FCJ after either treatment. After 3 days of refrigerated storage, the sensory scores of gamma- or UV-irradiated FCJ were superior to those of the control. The results indicate that both non-thermal treatments were effective in improving storage stability and extending shelf-life, but gamma irradiation was slightly better in suppressing microbial growth after treatment.</P> <P><B>Highlights</B></P><P>► Gamma irradiation and UV treatment were compared for their efficacy in sanitizing fresh carrot juice. ► Both treatments were effective in reducing the number of total aerobic bacteria but gamma irradiation was more effective. ► Reduction of ascorbic acid content was greater by gamma irradiation than by UV treatment. ► Sensory scores of gamma irradiated or UV-treated carrot juice were superior to those of the control.</P>

Growth and Mineral Contents of Spinach (Spinacia oleracea L.) and Radish (Raphanus sativus L.) as Related with a Low Dose Gamma Irradiation

( Jun Kwon Hwangbo ),( Jae Sung Kim ),( Ji Hyeok Lim ),( Myung Hwa Baek ),( Byung Yeoup Chung ),( Jin Hong Kim ) 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.4

N/A This study was to evaluate the effects of gamma irradiation on the germination, nutrient concentrations and growth of spinach and radish. Both the spinach and radish seeds exhibited relatively higher germination rates in response to the low doses of gamma irradiation compared to the non-irradiated control. Leaf DW of the radish did not respond to gamma irradiation hut that of the spinach increased significantly in response to a gamma radiation of 4 Gy (P< 0.05). Leaf growth parameters of the spinach including the leaf area and SLA (leaf aredeaf dry weight) also demonstrated increased responses to gamma irradiation. USL (root dry weightkhoot dry weight), root DW and root length of the spinach exhibited a positive response to gamma irradiation while those of the radish did not. In contrast, SRL (root IengtWroot dry weight) significantly decreased with gamma irradiation at 8 Gy for the spinach, hut not for the radish. The tissue nitrogen concentrations of the spinach showed an increased response to gamma irradiation while that of the radish did not. Furthermore, higher concentrations of phosphorus, potassium, calcium and magnesium were found in the irradiated spinach, hut not in the irradiated radish. It seems that the non-specific physiological and/ or biochemical activities of spinach might he accelerated by gamma irradiation, possibly accounting for the stimulation of nutrient uptake from the root media and early bio-mass accumulation in the current study.

Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

Kim, Joong-Sun,Jang, Hyosun,Bae, Min-Ji,Shim, Sehwan,Jang, Won-Seok,Lee, Sun-Joo,Park, Sunhoo,Lee, Seung-Sook The Korean Association for Radiation Protection 2017 방사선방어학회지 Vol.42 No.4

Background: The effects of radiation on tissues vary depending on the radiation type. In this study, a minipig model was used to compare the effects of ${\beta}$-rays from $^{166}Ho$ and ${\gamma}$-rays from $^{60}Co$ on the skin. Materials and Methods: In this study, the detrimental effects of ${\beta}$- and ${\gamma}$-irradiation on the skin were assessed in minipigs. The histopathological changes in the skin from 1 to 12 weeks after exposure to 50 Gy of either ${\beta}$- (using $^{166}Ho$ patches) or ${\gamma}$- (using $^{60}Co$) irradiation were assessed. Results and Discussion: The skin irradiated by ${\beta}$-rays was shown to exhibit more severe skin injury than that irradiated by ${\gamma}$-rays at 1-3 weeks post-exposure; however, while the skin lesions caused by ${\beta}$-rays recovered after 8 weeks, the ${\gamma}$-irradiated skin lesions were not repaired after this time. The observed histopathological changes corresponded with gross appearance scores. Seven days post-irradiation, apoptotic cells in the basal layer were detected more frequently in ${\beta}$-irradiated skin than in ${\gamma}$-irradiated skin. The basal cell density and skin thickness gradually decreased until 4 weeks after ${\gamma}$- and ${\beta}$- irradiation. In ${\beta}$-irradiated skin lesions, and the density and thickness increased sharply back to control levels by 6-9 weeks. However, this was not the case in ${\gamma}$-irradiated skin lesions. In ${\gamma}$-irradiated skin, cyclooxygenase-2 (COX-2) was shown to be expressed in the epidermis, endothelial cells of vessels, and fibroblasts, while ${\beta}$-irradiated lesions exhibited COX-2 expression that was mostly limited to the epidermis. Conclusion: In this study, ${\beta}$-rays were shown to induce more severe skin injury than ${\gamma}$-rays; however, the ${\beta}$-rays-induced injury was largely repaired over time, while the ${\gamma}$-rays-induced injury was not repaired and instead progressed to necrosis. These findings reveal the differential effects of ${\gamma}$- and ${\beta}$-irradiation on skin and demonstrate the use of minipigs as a beneficial experimental model for studying irradiation-induced skin damage.

Change in the enzymatic dual function of the peroxiredoxin protein by gamma irradiation

An, Byung Chull,Lee, Seung Sik,Lee, Jae Taek,Park, Chul-Hong,Lee, Sang Yeol,Chung, Byung Yeoup Elsevier 2012 Radiation physics and chemistry Vol.81 No.8

<P><B>Abstract</B></P><P>PP1084 protein was exposed to gamma irradiation ranging from 5 to 500kGy. Native PAGE showed minor structural changes in PP1084 at 5kGy, and major structural changes at >15kGy. Size-exclusion chromatography (SEC) showed the formation of a new shoulder peak when the protein was irradiated with 15 and 30kGy, and a double peak appeared at 100kGy. The results of PAGE and SEC imply that PP1084 protein is degraded by gamma irradiation, with simultaneous oligomerization. PP1084 chaperone activity reached the highest level at 30kGy of gamma irradiation, and then, decreased in a dose-dependent manner with increasing gamma irradiation. However, the peroxidase activity significantly decreased following exposure to all intensities of gamma irradiation. The improvement of chaperone activity using gamma irradiation might be promoted by the oligomeric structures containing covalently cross-linked amino acids. Consequently, PP1084 modification using gamma irradiation could elevate chaperone activity by about 3–4 folds compared to the non-irradiated protein.</P> <P><B>Highlights</B></P><P><B>►</B> The structure of PP1084 protein was drastically changed above 15kGy gamma irradiation. <B>►</B> PP1084 chaperone activity reached the highest level at 30kGy of gamma irradiation. <B>►</B> PP1084 modification using gamma irradiation could elevate chaperone activity by about 3–4 folds.</P>

Hesperidin structurally modified by gamma irradiation induces apoptosis in murine melanoma B16BL6 cells and inhibits both subcutaneous tumor growth and metastasis in C57BL/6 mice

Byun, Eui-Baek,Kim, Hye-Min,Song, Ha-Yeon,Kim, Woo Sik Elsevier 2019 Food and chemical toxicology Vol.127 No.-

<P><B>Abstract</B></P> <P>Hesperidin is a flavonoid which occurs in citrus fruits. Hesperidin was gamma-irradiated at doses of 0, 30, 70, and 150 kGy. Gamma irradiation induced a decreased hesperidin peak, and a new radiolytic peak that gradually increased up to 150 kGy. The new radiolytic peak was fractionated, and the fractionated hesperidin derivative was used for subsequent experiments. Hesperidin gamma-irradiated at 150 kGy was toxic toward B16BL6 cells, but not toward bone marrow-derived macrophages. This cytotoxicity was exerted via induction of apoptosis, as reflected by the high population of double-positive cells, increased sub-G1 phase cells, depolarization of matrix metalloproteinase, production of reactive oxygen species, weakness of cell adhesion, changes in cell morphology, and inhibition of B16BL6 cell migration. Furthermore, 150 kGy gamma-irradiated hesperidin decreased the expression of Bcl-2 and pro-caspases-3 and -9, increased the expression of Bax and cytosolic cytochrome <I>c</I>, and increased the cleavage of poly ADP ribose polymerase. <I>In vitro</I> mechanistic study revealed that 150 kGy gamma-irradiated hesperidin achieved significantly greater inhibition of lung metastasis and growth of melanoma B16BL6 cells in C57BL/6 mice than non-irradiated intact hesperidin did. These results suggest that the structural modification of hesperidin induced by gamma irradiation could facilitate the development of anti-cancer drugs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Gamma irradiation induced structural transformation of hesperidin. </LI> <LI> Gamma-irradiated hesperidin effectively induced apoptosis than unirradiated-hesperidin in B16BL6 melanoma cells through mitochondrial pathway. </LI> <LI> Gamma-irradiated hesperidin exhibited preventive and therapeutic effects in lung metastasis and tumor growth mice model. </LI> <LI> Gamma-irradiated hesperidin might be a candidate for cancer therapy. </LI> </UL> </P>

Gamma Irradiation Promotes the Growth Rate of Thai Pigmented Rice As Well As Inducing the Accumulation of Bioactive Compounds and Carbohydrate Hydrolyzing Enzymes Inhibitors (α-Glucosidase and α-Amylase) under Salt Conditions

Manatchanok Kongdin,Saowapa Chumanee,Sompong Sansenya 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.4

Rice contains many bioactive compounds that perform various biological activities. Some of these compounds have been identified as α-glucosidase and α-amylase inhibitors, including guaiacol, vanillin, methyl vanillate, vanillic acid, syringic acid, and 2-pentyl furan. In this study, we assessed the growth rate, photosynthetic pigment content, phenolic content, and flavonoid content of gamma-irradiated Thai pigmented rice. Bioactive components of gamma-irradiated rice that had been subjected to salt treatment were also investigated. The findings showed that production of photosynthetic pigments, which are associated with plant growth, was induced by low gamma exposure. Phenolic and flavonoid content of rice was increased after gamma irradiation at 5 to 1,000 Gy. Both gamma irradiation and the salt conditions changed the quantity of vanillin, methyl vanillate, and vanillic acid in the rice. However, at a salt concentration of 40 mM, the salt stress had more of an effect than the gamma dosage. However, the high concentrations of methyl vanillate and vanillic acid detected in the rice under salt conditions were ameliorated by gamma irradiation. Guaiacol served as the substrate of guaiacol peroxidase for catalyzed reactive oxygen species, as evidenced by the observation that the guaiacol content of rice decreased between increased gamma dosages. A gamma dose of 40 to 1,000 Gy resulted in the production of syringic acid. Under salt stress, syringic acid buildup was also seen to be ameliorated by gamma irradiation. In comparison to salt conditions, particularly for 20 mM salt, gamma irradiation had less of an impact on the 2-pentyl furan in rice.

Effects of irradiation source and dose level on quality characteristics of processed meat products

Ham, Y.K.,Kim, H.W.,Hwang, K.E.,Song, D.H.,Kim, Y.J.,Choi, Y.S.,Song, B.S.,Park, J.H.,Kim, C.J. Pergamon 2017 Radiation physics and chemistry Vol.130 No.-

<P>The effect of irradiation source (gamma-ray, electron-beam, and X-ray) and dose levels on the physicochemical, organoleptic and microbial properties of cooked beef patties and pork sausages was studied, during 10 days of storage at 30 +/- 1 degrees C. The processed meat, products were irradiated at 0, 2.5, 5, 7.5, and 10 kGy by three different irradiation sources. The pH of cooked beef patties and pork sausages was unaffected by irradiation sources or their doses. The redness of beef patties linearly decreased with increasing dose level (P < 0.05), obviously by e-beam irradiation compared to gamma-ray and X-ray (P < 0.05). The redness of pork sausages was increased by gamma-ray irradiation, whereas it decreased by e-beam irradiation depending on absorbed dose level. No significant changes in overall acceptability were observed for pork sausages regardless of irradiation source (P> 0.05), while gamma-ray irradiated beef patties showed significantly decreased overall acceptability in a dose-dependent manner (P < 0.05). Lipid oxidation of samples was accelerated by irradiation depending on irradiation sources and dose levels during storage at 30 degrees C. E-beam reduced total aerobic bacteria of beef patties more effectively, while gamma-ray considerably decreased microbes in pork sausages as irradiation dose increased. The results of this study indicate that quality attributes of meat products, in particular color, lipid oxidation, and microbial properties are significantly influenced by the irradiation sources. (C) 2016 Published by Elsevier Ltd.</P>

Effects of Gamma Irradiation on Chemical Composition, Antinutritional Factors, Ruminal Degradation and In vitro Protein Digestibility of Full-fat Soybean

Taghinejad, M.,Nikkhah, A.,Sadeghi, A.A.,Raisali, G.,Chamani, M. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.4

The aim of this study was to evaluate the effects of gamma irradiation (${\gamma}$-irradiation) at doses of 15, 30 and 45 kGy on chemical composition, anti-nutritional factors, ruminal dry matter (DM) and crude protein (CP) degradibility, in vitro CP digestibility and to monitor the fate of true proteins of full-fat soybean (SB) in the rumen. Nylon bags of untreated or ${\gamma}$-irradiated SB were suspended in the rumens of three ruminally-fistulated bulls for up to 48 h and resulting data were fitted to a nonlinear degradation model to calculate degradation parameters of DM and CP. Proteins of untreated and treated SB bag residues were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Digestibility of rumen undegraded CP was estimated using the three-step in vitro procedure. The chemical composition of raw and irradiated soybeans was similar. Results showed that phytic acid in ${\gamma}$-irradiated SB at dose of 30 kGy was eliminated completely. The trypsin inhibitor activity of 15, 30 and 45 kGy ${\gamma}$-irradiated SB was decreased (p<0.01) by 18.4, 55.5 and 63.5%, respectively. From in sacco results, ${\gamma}$-irradiation decreased (p<0.05) the washout fractions of DM and CP at doses of 30 and 45 kGy, but increased (p<0.05) the potentially degradable fractions. Gamma irradiation at doses of 15, 30 and 45 kGy decreased (p<0.05) effective degradability of CP at a rumen outflow rate of 0.05 $h^{-1}$ by 4.4, 14.4 and 26.5%, respectively. On the contrary, digestibility of ruminally undegraded CP of irradiated SB at doses of 30 and 45 kGy was improved (p<0.05) by 12 and 28%, respectively. Electrophoretic analysis of untreated soybean proteins incubated in the rumen revealed that ${\beta}$-conglycinin subunits had disappeared at 2 h of incubation time, whereas the subunits of glycinin were more resistant to degradation until 16 h of incubation. From the SDS-PAGE patterns, acidic subunits of 15, 30 and 45 kGy ${\gamma}$-irradiated SB disappeared after 8, 8 and 16 h of incubation, respectively, while the basic subunits of glycinin were not degraded completely until 24, 48 and 48 h of incubation, respectively. It was concluded that ${\gamma}$-irradiated soybean proteins at doses higher than 15 kGy could be effectively protected from ruminal degradation.

Alterations in Seed Vigour and Viability of Soybean Related with Accelerated Seed Aging and Low Dose Gamma Irradiation

Jun-Kwon Hwangbo,Jae-Sung Kim,Ji Hyeok Lim,Myung Hwa Baek,Byung Yeoup Chung 韓國作物學會 2003 Korean journal of crop science Vol.48 No.4

The objective of this study was to demonstrate whether or not the deleterious effects of accelerated aging on seed vigour and viability are alleviated by interaction with gamma irradiation. Seeds of soybean (Glycine max L.) were artificially aged and subsequently irradiated with 4 and 8 Gy of gamma irradiation. Germination rate was negatively affected by accelerated aging and positively by gamma irradiation, with a positive interaction of a 3day-seed aging treatment occurring with 4 Gy, possibly suggesting that 4 Gy of gamma irradiation partially offset the adverse effects of seed aging on germination. However, 5-day aged seeds did not gain any benefits from the gamma irradiation. Electrolyte leakage from the seeds increased with the duration in days aged. Irradiation, however, did not impose any effects on the leakage. Respiration rate of the seed with hypocotyl and primary root was significantly low for the aged seeds, but not for the seeds with both irradiation and aging treatments. Accelerated aging decreased the dry weight of the hypocotyl and primary root of the seeds without any measurable effects of irradiation. α -Amylase activity decreased with seed aging and positively responded to gamma irradiation. The data is discussed with regard to the possible roles of gamma irradiation for improving the seed vigour and viability of aged seeds.