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      • Cloning and expression analysis of the <i>Ccrboh</i> gene encoding respiratory burst oxidase in <i>Citrullus colocynthis</i> and grafting onto <i>Citrullus lanatus</i> (watermelon)

        Si, Ying,Dane, Fenny,Rashotte, Aaron,Kang, Kwonkyoo,Singh, Narendra K. Oxford University Press 2010 Journal of experimental botany Vol.61 No.6

        <P>A full-length drought-responsive gene <I>Ccrboh</I>, encoding the respiratory burst oxidase homologue (rboh), was cloned in <I>Citrullus colocynthis</I>, a very drought-tolerant cucurbit species. The robh protein, also named NADPH oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ROS). The <I>Ccrboh</I> gene accumulated in a tissue-specific pattern when <I>C</I>. <I>colocynthis</I> was treated with PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCl, while the homologous <I>rboh</I> gene did not show any change in <I>C</I>. <I>lanatus</I> var. <I>lanatus</I>, cultivated watermelon, during drought. Grafting experiments were conducted using <I>C</I>. <I>colocynthis</I> or <I>C</I>. <I>lanatus</I> as the rootstock or scion. Results showed that the rootstock significantly affects gene expression in the scion, and some signals might be transported from the root to the shoot. <I>Ccrboh</I> in <I>C</I>. <I>colocynthis</I> was found to function early during plant development, reaching high mRNA transcript levels 3 d after germination. The subcellular location of Ccrboh was investigated by transient expression of the 35S::Ccrboh::GFP fusion construct in protoplasts. The result confirmed that Ccrboh is a transmembrane protein. Our data suggest that <I>Ccrboh</I> might be functionally important during the acclimation of plants to stress and also in plant development. It holds great promise for improving drought tolerance of other cucurbit species.</P>

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        Morphological characterization of Korean and Turkish watermelon germplasm

        Huh, Yun Chan,Choi, Hak Soon,Solmaz, Ilknur,Sari, Nebahat,Kim, Su Institute of Agricultural Science 2014 Korean Journal of Agricultural Science Vol.41 No.4

        A total of 67 watermelon accessions which include 37 accessions from Korean and 27 accessions from Turkish germplasm and 3 accessions of other related species from USA were investigated for morphological characteristics. The UPOV descriptor list for 56 characters (6 seedlings, 4 plants, 11 leaves, 5 flowers, 23 fruits and 7 seeds) was used in characterization. In addition, eight quantitative characters, hypocotyl length, cotyledon width, cotyledon length, fruit weight, fruit length, fruit width, thickness of outer layer of pericarp and soluble solid content were also measured. The 56 qualitatively scored characters were analyzed by principle coordinate analysis (PCoA) while the eight quantitative ones were subjected to principle component analysis (PCA). Morphological characterization result demonstrated that the accessions displayed high morphological diversity(how much percent?). A high level of phenotypic diversity was observed from the results of morphological characterization. However, plant growth habit and leaf blade flecking showed constant characters for all of the accessions. The Korean and Turkish watermelon genotypes are diverse groups and can be separated by both multivariate analysis of morphological characters although the grouping was more apparent in PCoS results.

      • Morphological characterization of Korean and Turkish watermelon germplasm

        Yun Chan Huh,Hak Soon Choi,Ilknur Solmaz,Nebahat Sari,Su Kim 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4

        A total of 67 watermelon accessions which include 37 accessions from Korean and 27 accessions from Turkish germplasm and 3 accessions of other related species from USA were investigated for morphological characteristics. The UPOV descriptor list for 56 characters (6 seedlings, 4 plants, 11 leaves, 5 flowers, 23 fruits and 7 seeds) was used in characterization. In addition, eight quantitative characters, hypocotyl length, cotyledon width, cotyledon length, fruit weight, fruit length, fruit width, thickness of outer layer of pericarp and soluble solid content were also measured. The 56 qualitatively scored characters were analyzed by principle coordinate analysis (PCoA) while the eight quantitative ones were subjected to principle component analysis (PCA). Morphological characterization result demonstrated that the accessions displayed high morphological diversity(how much percent?). A high level of phenotypic diversity was observed from the results of morphological characterization. However, plant growth habit and leaf blade flecking showed constant characters for all of the accessions. The Korean and Turkish watermelon genotypes are diverse groups and can be separated by both multivariate analysis of morphological characters although the grouping was more apparent in PCoS results.

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