Analgesic and anti-inflammatory effect of theaqueous extract of Angelica dahurica

최인호,임형호,송윤경 대한한의학회 2008 대한한의학회지 Vol.29 No.2

Background: Angelica dahurica has been used in various clinical cases. Its taste is hot and its property is warm, dry and nonpoisonous. Its efficacy is to remove wind-damp, cure swelling and edema, exhaust pus, stop itching, rhinitis and leukorrhea. Object: To test through experiment Angelica dahurica's analgesic and anti-inflammatory efficacy. Method: Inject acetic acid as a pain-inducing substance to the mice and measure visceral pain bywrithing reflex. Inject carrageenan that is an edema-inducing substance to the rat's paw and measure volume of edema. Take thermal pain to mice with plantar test and measure paw withdrawal latency. Normal group is non Angelica dahurica-treated group and treated group is Angelica dahurica-treated group. Results: In acetic acid-induced visceral model, treatment with Angelica dahurica suppressed writhing reflex signifi- cantlyand dose-dependently. In carrageenan-induced paw edema model, treatment with Angelica dahurica suppressed carrageenan-induced paw edema. In plantar test model, no significant effect on the withdrawal latency of thermal stimulation-induced nociception was observed. Conclusion: Angelica dahurica has analgesic and anti-inflammatory efficacy. Background: Angelica dahurica has been used in various clinical cases. Its taste is hot and its property is warm, dry and nonpoisonous. Its efficacy is to remove wind-damp, cure swelling and edema, exhaust pus, stop itching, rhinitis and leukorrhea. Object: To test through experiment Angelica dahurica's analgesic and anti-inflammatory efficacy. Method: Inject acetic acid as a pain-inducing substance to the mice and measure visceral pain bywrithing reflex. Inject carrageenan that is an edema-inducing substance to the rat's paw and measure volume of edema. Take thermal pain to mice with plantar test and measure paw withdrawal latency. Normal group is non Angelica dahurica-treated group and treated group is Angelica dahurica-treated group. Results: In acetic acid-induced visceral model, treatment with Angelica dahurica suppressed writhing reflex signifi- cantlyand dose-dependently. In carrageenan-induced paw edema model, treatment with Angelica dahurica suppressed carrageenan-induced paw edema. In plantar test model, no significant effect on the withdrawal latency of thermal stimulation-induced nociception was observed. Conclusion: Angelica dahurica has analgesic and anti-inflammatory efficacy.

Analgesic and anti-inflammatory effect of the aqueous extract of Angelica dahurica

Choi, In-Ho,Song, Yun-Kyung,Lim, Hyung-Ho The Society of Korean Medicine 2008 대한한의학회지 Vol.29 No.2

Background: Angelica dahurica has been used in various clinical cases. Its taste is hot and its property is warm, dry and nonpoisonous. Its efficacy is to remove wind-damp, cure swelling and edema, exhaust pus, stop itching, rhinitis and leukorrhea. Object: To test through experiment Angelica dahurica's analgesic and anti-inflammatory efficacy. Method: Inject acetic acid as a pain-inducing substance to the mice and measure visceral pain bywrithing reflex. Inject carrageenan that is an edema-inducing substance to the rat's paw and measure volume of edema. Take thermal pain to mice with plantar test and measure paw withdrawal latency. Normal group is non Angelica dahurica-treated group and treated group is Angelica dahurica-treated group. Results: In acetic acid-induced visceral model, treatment with Angelica dahurica suppressed writhing reflex significantlyand dose-dependently. In carrageenan-induced paw edema model, treatment with Angelica dahurica suppressed carrageenan-induced paw edema. In plantar test model, no significant effect on the withdrawal latency of thermal stimulation-induced nociception was observed. Conclusion: Angelica dahurica has analgesic and anti-inflammatory efficacy.

Antioxidant Activity and Irritation Test of Extracts Obtained from Angelica dahurica

Yang-Suk Lee,Nam-Woo Kim 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.1

In this study, we assessed the free radical scavenging and xanthine oxidase inhibitory activities of extracts isolated from the dried roots and stems (including leaves) of Angelica dahurica. The irritation response from these extracts was also assessed to determine potential cosmetic use. Both sources of A. dahurica extracts exhibited radical scavenging properties to different extents. The free radical scavenging potency (EC50) of the stems (including leaves) of A. dahurica was 243.33 ㎍/mL, which is significantly lower (p<0.01) than that observed of the roots (1,161.79 ㎍/mL). The inhibition values (EC50) of xanthine oxidase were as follows: 435.19 ㎍/mL (roots) and 434.66 ㎍/mL (stems). We noted no significant differences between the two plant parts with regard to ability to inhibit xanthine oxidase activity. After the application of A. dahurica extracts to rabbits for skin and eye irritation tests, no negative effects were observed; therefore, the extracts are considered to be non-irritating to the skin and eye.

Antioxidant Activity and Irritation Test of Extracts Obtained from Angelica dahurica

Lee, Yang-Suk,Kim, Nam-Woo The Korean Society of Food Science and Nutrition 2011 Preventive Nutrition and Food Science Vol.16 No.1

In this study, we assessed the free radical scavenging and xanthine oxidase inhibitory activities of extracts isolated from the dried roots and stems (including leaves) of Angelica dahurica. The irritation response from these extracts was also assessed to determine potential cosmetic use. Both sources of A. dahurica extracts exhibited radical scavenging properties to different extents. The free radical scavenging potency ($EC_{50}$) of the stems (including leaves) of A. dahurica was 243.33 ${\mu}g/mL$, which is significantly lower (p<0.01) than that observed of the roots (1,161.79 ${\mu}g/mL$). The inhibition values ($EC_{50}$) of xanthine oxidase were as follows: 435.19 ${\mu}g/mL$ (roots) and 434.66 ${\mu}g/mL$ (stems). We noted no significant differences between the two plant parts with regard to ability to inhibit xanthine oxidase activity. After the application of A. dahurica extracts to rabbits for skin and eye irritation tests, no negative effects were observed; therefore, the extracts are considered to be non-irritating to the skin and eye.

Pharmacological Activities of Water Extracts of Umbelliferae Plants

Kim, Chang-Min,Heo, Moon-Young,Kim, Hyun-Pyo,Sin, Kwan-Seog,Pachaly, Peter The Pharmaceutical Society of Korea 1991 Archives of Pharmacal Research Vol.14 No.1

In order to evaluate the pharmacological activities of Chinese medicine, nine Umbelliferae plants were selected and their restoring activity against dexamethasone-induced disorders, liver protective activity, antimicrobial activity, anti-inflammatory activity and antimutagenic activity were tested and compared. Angelica dahurica. Angelica acutiloba and Ostericum koreanum showed various activities in these tests at the dose used in this study.

The Effects of Isoimperatorin Isolated from Angelicae dahuricae on Cyclooxygenase-2 and 5-Lipoxygenase in Mouse Bone Marrow-derived Mast Cells

Moon, Tae-Chul,Jin, Mei-Hua,Son, Jong-Keun,Chang, Hyeun-Wook 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2

isoimperatorin (4-[(3-Methyl-2-butenyl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one) is a medicinal herbal product that is isolated from the dried roots of Angelicae dahuricae. Isoimperatorin inhibits the cyclooxygenase-2 (COX-2) and COX-1-dependent phases of prostaglandin $D_2\;(PGD_2)$ generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner, with $IC_{50}$ values of 10.7 ${\mu}M$ and 24 ${\mu}M$, respectively. However, this compound was not able to inhibit COX-1 and 2 protein expression in BMMC that were treated with concentrations of up to 50 ${\mu}M$, which indicates that isoimperatorin directly inhibits COX-2 activity. Furthermore, this compound consistently inhibited the production of leukotriene $C_4\;(LTC_4)$, as well as the degranulation reaction in BMMC, with an $IC_{50}$ value of 5.7 ${\mu}M$ and 9 ${\mu}M$, respectively, and these effects occurred in a dose dependent fashion. These results demonstrate that isoimperatorin has a dual cyclooxygenase-2 selective/5-lipoxygenase inhibitory activity, and therefore may provide the basis for novel anti-inflammatory drugs.

구릿대 건전 종묘 생산을 위한 채종 적기 구명과 트레이 육묘 방법

남효훈(Hyo Hoon Nam),김광섭(Kwang Seop Kim),김병성(Beung Sung Kim) 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.3

Background: Angelica dahurica is a perennial plant of the Apiaceae family, and its root is used as a medicine named Baek-Ji. However, there are no registered varieties and seed production systems to date. This study was carried out to investigate the proper seed harvest time and seedling raising method for plug seedling production in A. dahurica. Methods and Results: Seeds were harvested at approximately five-day intervals from 5 to 60 days after the flowering period, and seed yield and 1,000 grain weight were determined. For plug seedling production, seedlings were grown in different plug cell sizes and for different raising periods. Angelica dahurica flowered on August 1, and the seed yield was the highest at 40 days after flowering. Furthermore, 1,013℃ of accumulated temperature from the flowering period was required to obtain the maximum seed yield. Seedling growth by raising method tended to increase as cell size and raising period increased. The change of dry weight according to cell size and days after seeding were well expressed with an exponential function. Root balls were formed 63 days after seeding. However, there was no difference in the rooting rate and root yield based on the seedling raising method used. Conclusions: Seeds of A. dahuica should be harvested 40 days after flowering to obtain the highest yield. On hundred five cells per tray and 63 days of raising were appropriate for optimum seedling raising efficiency and ease of transplanting.

The Effects of Isoimperatorin Isolated from Angelicae dahuricae on Cyclooxygenase-2 and 5-Lipoxygenase in Mouse Bone Marrow-derived Mast Cells

Tae Chul Moon,김미화,손종근,장현욱 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2

Isoimperatorin (4-[(3-Methyl-2-butenyl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one) is a medicinal herbal product that is isolated from the dried roots of Angelicae dahuricae. Isoimperatorin inhibits the cyclooxygenase-2 (COX-2) and COX-1-dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner, with IC50 values of 10.7 μM and 24 μM, respectively. However, this compound was not able to inhibit COX-1 and 2 protein expression in BMMC that were treated with concentrations of up to 50 μM, which indicates that isoimperatorin directly inhibits COX-2 activity. Furthermore, this compound consistently inhibited the production of leukotriene C4 (LTC4), as well as the degranulation reaction in BMMC, with an IC50 value of 5.7 μM and 9 μM, respectively, and these effects occurred in a dose dependent fashion. These results demonstrate that isoimperatorin has a dual cyclooxygenase-2 selective/5-lipoxygenase inhibitory activity, and therefore may provide the basis for novel anti-inflammatory drugs.

Angelica^®의 피부 광노화에 대한 효과

진무현 ( Mu Hyun Jin ),정민환 ( Min-hwan Jung ),임영희 ( Young-hee Lim ),이상화 ( Sang Hwa Lee ),강상진 ( Sangjin Kang ),조완구 ( Wan-goo Cho ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.3

피부노화는 피부의 이상현상이나 질환은 아니며 나이가 들어감에 따라 시간의 진행에 따라 일어나는 퇴행성 변화로 외적형태의 변화로는 피부 건조 및 주름 등이 있다. 주름을 생성하는 원인에는 자외선, 건조 및 물리적, 화학적 자극 등 환경요인에 기인하는 피부 스트레스와 각질층의 수분량 저하, 비후화, 진피의 교원섬유, 탄력섬유의 양적, 질적 변화 등이 유발하는 피부의 탄력성이나 신축성의 저하를 들 수 있다. 본 연구에서는 Angelim dahurica root에서 얻어진 3종의 주름개선 유효성분의 구조분석 및 피부 노화에 대한 효과를 살펴보았다. Skin aging is not a disease nor an abnormal phenomenon but a collection of degenerative changes with age, characterized by skin dryness, wrinkle formation, and loss of skin elasticity. The skin wrinkles are caused by either genetically predisposed factors or environmental factors such as UV irradiation or physical/chemical stimulus. The histological manifestations of wrinkles are changes in both amount and integrity of elastic and collagen fibers. Here we report the isolation and characterization of 3 active compounds, prangenidin, 8-hydroxybergapten, and xanthotoxol from Angelica dahurica root. The anti-wrinkle activities of these compounds were also investigated.

Protective effects of a herbal extract combination of <i>Bupleurum falcatum</i>, <i>Paeonia suffruticosa</i>, and <i>Angelica dahurica</i> against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein

Sim, Yeomoon,Park, Gunhyuk,Eo, Hyeyoon,Huh, Eugene,Gu, Pil Sung,Hong, Seon-Pyo,Pak, Youngmi Kim,Oh, Myung Sook Elsevier 2017 NEUROSCIENCE Vol.340 No.-

<P><B>Abstract</B></P> <P>Parkinson’s disease (PD) is one of the progressive neurodegenerative diseases of whose condition is characterized by dopaminergic neuronal cell loss and dysfunction in the substantia nigra pars compacta (SNpc) and the striatum. Recent studies have demonstrated that the nuclear receptor-related 1 protein (Nurr1) is critical of dopaminergic phenotype induction in mesencephalic dopaminergic neurons. Further, Nurr1 engages in synthesizing and storing dopamine through regulating levels of tyrosine hydroxylase (TH), dopamine transporter (DAT) and vesicular monoamine transporter 2 (VMAT2). The aim of this study was to investigate the protective effects of a herbal extract combination, consisting of <I>Bupleurum falcatum</I>, <I>Paeonia suffruticosa</I>, and <I>Angelica dahurica</I> (MABH), on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD-like symptoms and to elucidate possible mechanisms of action focusing on Nurr1. In a subacute mouse model of MPTP-induced PD, MABH treatment resulted in recovery from movement impairments. MABH prevented dopamine depletion and protected against dopaminergic neuronal degradation induced by MPTP. Additionally, MABH increased Nurr1 expression in the SNpc of mice. To evaluate the effects of MABH on Nurr1 expression, we measured the protein levels of Nurr1 and its regulating factors using Western blot analysis in PC12 cells. MABH treatment induced the phosphorylation of extracellular signal-regulated kinase protein via increasing the protein expression levels of Nurr1 and ultimately the levels of TH, VMAT2, and DAT. These results indicate that MABH has protective effects on dopaminergic neurons in a mouse model of PD by regulating Nurr1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MABH improved MPTP-induced movement impairments in mice. </LI> <LI> MABH prevented dopamine depletion and protected against MPTP-induced dopaminergic neuron damage in mice. </LI> <LI> MABH increased Nurr1 expression in the SNpc of mice. </LI> <LI> MABH increased TH, DAT, and VMAT2 expressions by upregulating Nurr1 via phosphorylation of ERK in PC12 cells. </LI> </UL> </P>