Influence of the Lysine to Protein Ratio in Practical Diets on the Efficiency of Nitrogen Use in Growing Pigs

Lee, K.U.,Boyd, R.D.,Austic, R.E.,Ross, D.A.,Han, In K. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.6

Twelve gilts were used to investigate the effect of lysine to protein ratio (5.2 g lysine/100 g CP vs. 6.7 g lysine/100 g CP) in practical diets on nitrogen retention and the efficiency of utilization in growing pigs. Treatments involved 2 levels of dietary lysine (5.2 or 6.7 g/100 g CP) and 3 levels of dietary crude protein (11, 14 and 17% in diet). Nitrogen retention was greatest when pigs were fed the control diet containing 17% protein. Nitrogen retention progressively increased as dietary protein increased (p < 0.01), but it was not affected by lysine concentration (g/100 g CP). Apparent biological value (ABV, nitrogen retained/apparently digestible nitrogen) was estimated to be ~50% at the maximum nitrogen retention. ABV was not affected by lysine concentration, but declined (p < 0.05) as the dietary protein level increased. The efficiency of intake N used for maximum nitrogen retention was approximately 44%. One gram of lysine supported approximately 9 to 10 g apparent protein accretion (nitrogen retention ${\times}$ 6.25/lysine intake) in pigs fed control diets. The efficiency of lysine utilization for protein accretion was lower in pigs fed high-lysine diets (6.7 g lysine/l00 g CP) so that 1 g of lysine accounted for 7 to 8 g of protein accretion in these pigs (p < 0.01). The lysine required to support maximum nitrogen retention in pigs fed high-lysine diets was higher than that in pigs fed control diets, which suggests that lysine was over-fortified relative to crude protein, since practical diets can not be formulated without excess of some amino acids. In summary the concentration of 5.2 g total lysine/100 g CP in diet is more appropriate for corn-soybean diets than the commonly suggested the content of 6.7 g total lysine/100 g CP.

Comparison of the Efficiency of Absorbed Nitrogen Use from Different Protein Sources in Diets Having Similar Amino Acid Balance

Lee, K.U.,Boyd, R.D.,Austic, R.E.,Ross, D.A.,Han, In K. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.6

Nine crossbred female pigs fitted with the bladder catheters were used to investigate the effects of dietary protein form on the efficiency of absorbed nitrogen for nitrogen retention in growing pigs. Combinations of the main protein sources were corn-soybean meal (CSM; slow + slow absorption rate form), corn-hydrolyzed casein (CAS; slow + rapid absorption rate form) and corn-porcine plasma (CPL; slow + intermediate absorption rate form). All experimental diets were formulated to be isonitrogenous (CP 11%) and isocaloric (3.5 Mcal/kg) and synthetic amino acids were added to the diet as required to maintain an equivalent amino acid profile among diets. Fecal digestibility of nitrogen was not different among treatments (p > 0.10). Ingested nitrogen was absorbed with an apparent efficiency of 82% to 84%. Mean nitrogen retention in pigs fed the CSM diet was as high as for pigs fed the CPL diet (0.74 g N/kg $BW^{0.75}$ per d), which was higher than the N retention rate in pigs fed CAS diet (0.68 g/kg $BW^{0.75}$ per d; P < 0.05). Apparent biological values (ABV = 100 ${\times}$ N retention/absorbed nitrogen) were 63.3%, 58.0% and 61.6% for CSM, CAS, and CPL groups, respectively (p < 0.05). There was no difference in mean energy digestibility among treatments. The efficiency of absorbed lysine utilization was significantly different among treatments (p < 0.05). Pigs fed the CAS diet were inferior to counterparts on the other diets in utilizing absorbed lysine. The ratios of free (and small peptide-bound) to protein-bound amino acids in CSM diet differed considerably from the CAS diet. This may affect the efficiency of amino acids utilization for nitrogen retention if hydrolyzed and intact amino acid pools reach the blood at different times.

The Optimum Methionine to Methionine Plus Cystine Ratio for Growing Pigs Determined Using Plasma Urea Nitrogen and Nitrogen Balance

Qiao, Shiyan,Piao, Xiangshu,Feng, Zhanyu,Ding, Yuhua,Yue, Longyao,Thacker, P.A. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.3

The objective of this study was to determine the optimum ratio of methionine to methionine plus cystine for growing pigs. A nitrogen balance trial was conducted using a total of 21 barrows (Large WhiteLandrace) over two replicates. The initial body weight was $20.36{\pm}1.22kg$ (mean${\pm}$SD) in the first replicate and $23.54{\pm}1.02kg$ (mean${\pm}$SD) in the second. For each replicate, the 21 pigs were randomly assigned to one of seven dietary treatments with three observations per treatment. The diets included a methionine and cystine-deficient basal diet with all other essential nutrients meeting nutrient requirements and six diets formulated with graded levels of DL-methionine (0.00, 0.03, 0.06, 0.10, 0.13, 0.16%) and $L-Cystine{\cdot}HCl{\cdot}H_2O$ (0.19, 0.15, 0.11, 0.07, 0.04, 0.00%). This resulted in ratios of methionine to methionine plus cystine of 41.3, 29.6, 35.3, 41.2, 46.0, 51.6 and 57.5%. Each experimental period lasted 12 days consisting of a seven-day adaptation period followed by a five-day total collection of urine and feces. During the collection period, pigs were fed 900 g/day for the first replicate and 1,200 g/day for the second replicate. The feed was provided in three equal portions at 0800, 1500, and 2200 h daily. Pigs had ad libitum access to water after feeding. There was a linear (p<0.01) and quadratic (p<0.01) effect on daily gain and feed conversion as the ratio of methionine to methionine plus cystine increased. Pigs receiving the diets providing a methionine to methionine plus cystine ratio of 51.6% had the best daily gain and feed conversion. Plasma urea nitrogen was also lowest for this treatment. Nitrogen retention increased (p<0.01) as the relative proportion of methionine increased up to 51.6% and then a downward trend occurred at 57.5%. The quadratic regression model, as well as one- and two- slope regression line models, were used to determine the optimum ratio of methionine to methionine plus cystine. Eliminating the 35.3% methionine to methionine plus cystine treatment resulted in $R^2$ values in excess of 0.92. The optimal ratio of methionine to methionine plus cystine was estimated to be 54.15% for nitrogen retention and 56.72% for plasma urea nitrogen.

The Effect of Lysine to Protein Ratio on Growth Performance and Efficiency of Nitrogen Utilization in Pigs

Li, Defa,Xi, Pengbin,Wang, Junxun,Wang, Jitan,Ren, Jiping,Kang, Yufan,Thacker, P. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.9

One feeding trial and two metabolic trials were conducted to investigate the effects of lysine to protein ratio in practical swine diets on growth performance and efficiency of nitrogen retention and utilization in different growing phases. In Trial one (the feeding trial), 90 mixed sex pigs weighing $9.1{\pm}1.4kg$ (Duroc ${\times}$ Landrance ${\times}$ Beijing Black) were used to study the effects of concentrations of 5.2, 5.3, 5.8, 6.4 and 7.2 g lysine/100 g CP in diets containing 1.2% lysine on growth performance and serum urea nitrogen. The results showed that feed conversion efficiency and economic efficiency were best for pigs fed the diet containing the lysine concentration of 5.8 g /100 g crude protein. Serum urea nitrogen concentration decreased linearly (p=0.0009) and serum free lysine content increased linearly (p=0.0017) as the lysine to protein ratio in diets increased from 5.2 to 7.2 g/100 g. In Trials two and three (the metabolic trials), five growing barrows (Duroc ${\times}$ Landrance ${\times}$ Beijing black), with initial body weights of approximately $26{\pm}2.4kg$ and $56.3{\pm}3.5kg$, respectively, were allotted to five dietary treatments according to a $5{\times}5$ Latin square design. Trial two contained 5.2, 5.7, 6.1, 6.7 and 6.8 g lysine/100 g CP treatments. Trial three contained 4.6, 5.0, 5.6, 6.1 and 6.6 g lysine/100 g CP treatments. The results showed that nitrogen retention in growing pigs decreased linearly (p=0.0011 in Trial two; p=0.0099 in Trial three) as the lysine to protein ratio in diets increased. The ratio of lysine to protein in diets resulting in maximum nitrogen retention was 5.2 g/100 g and 5.0 g/100 g in Trial two and Trial three, respectively. In Trial two, apparent biological value and gross nitrogen efficiency increased linearly (p=0.0135 and p=0.0192, respectively) as the lysine to protein ratio increased from 5.2 to 6.8 g lysine/100 g CP. In summary, we concluded that the optimal Lysine to Protein Ratios for 8-20 kg and 20-80 kg pigs were 5.8 g/100 g and 5.0 to 5.2 g/100 g, respectively.

QSPR Models for Chromatographic Retention of Some Azoles with Physicochemical Properties

Polyakova, Yulia,Jin, Long Mei,Row, Kyung-Ho Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.2

This work deals with 24 substances composed of nitrogen-containing heterocycles. The relationships between the chromatographic retention factor (k) and those physicochemical properties which are relevant in quantitative structure-properties relationship (QSPR) studies, such as the polarizability $(\alpha)$, molar refractivity (MR), lipophilicity (logP), dipole moment $(\mu)$, total energy $(E_{tot})$, heat of formation $(\Delta H_f)$, molecular surface area $(S_M)$, and binding energy $(E_b)$, were investigated. The accuracy of the simple linear regressions between the chromatographic retention and the descriptors for all of the compounds was satisfactory (correlation coefficient, $0.8 \leq r \leq 1.0$). The QSPR models of these nitrogen-containing heterocyclic compounds could be predicted with a multiple linear regression equation having the statistical index, r = 1.000. This work demonstrated the successful application of the multiple linear approaches through the development of accurate predictive equations for retention factors in liquid chromatography.

사료 내 조농비율에 따른 생균제의 첨가가 흑염소의 사료섭취량, 영양소 소화율 및 질소축적에 미치는 영향

황보순,조익환,송기준,이성훈 한국유기농업학회 2007 韓國有機農業學會誌 Vol.15 No.2

This study was conducted to determine effects of probiotics on performance, nutrient digestibility and nitrogen retention in Korean black goats fed two diets differing in forage to concentrate (F:C) ratio (30:70 and 70:30), for the establishment of their more efficient feeding management system. The probiotics employed in this trial were mixtures of different microbial species, which consisted of Lactobacillus casei, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae and Streptomyces griseus. Additional levels of probiotics to each F:C ratio (70:30 or 30:70) were 0 and 0.2%, respectively. Thus, twelve Korean black male goats were allotted to treatments in four groups of three goats per treatment and then they were housed in individual metabolism cages with a randomized complete block design for 21 days. Dry matter (DM) intakes were not affected by dietary F:C ratio and probiotics. Digestible DM amounts were significantly (p<0.05) decreased with increasing levels of dietary forage, but they were not affected by probiotics addition. Dry matter intakes per metabolic body weight and their ratio per body weight had a similar trend to DM intakes with no significant difference by F:C ratio and probiotics addition. The nutrient digestibility was significantly (p<0.05) increased with decreasing levels of forage in the diet, but it was not affected by probiotics addition. Urinary nitrogen loss was significantly (p<0.05) decreased with decreasing levels of dietary forage, but there was no significant difference between probiotics-supplemented and unsupplemented groups within the same F:C ratio. On the contrary, nitrogen retention was decreased with increasing levels of dietary forage, and probiotics supplementation to two diets differing in F:C ratio showed slightly increasing trends in the nitrogen retention. From the above results, probiotics supplementation to two diets differing in F:C ratio did not have the significant influence on feed intakes, nutrient digestibility and nitrogen retention. Consequently, these parameters of Korean black goats were dominated rather by F:C ratio than by dietary probiotics.

Effect of Potato By-products Based Silage on Rumen Fermentation, Methane Production and Nitrogen Utilization in Holstein Steers

Pen, B.,Iwama, T.,Ooi, M.,Saitoh, T.,Kida, K.,Iketaki, T.,Takahashi, J.,Hidari, H. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9

The effect of substituting potato by-products based silage (PBS) for concentrates on ruminal fermentation, methane production and nitrogen utilization in Holstein steers was evaluated. Three growing Holstein steers ($490{\pm}19kg$, initial body weight) were used in a $3{\times}3$ Latin square experiment with three experimental diets in which PBS was included at (1) 0, (2) 19 and (3) 27%, on a dry matter basis, replacing concentrates and wheat bran. Increasing replacement levels of PBS slightly increased dry matter (DM), organic matter (OM), NDF and ADF intakes by the steers (p<0.05). Inclusion of PBS at 19% increased crude protein (CP) digestibility of the experimental diets compared with the control (p<0.05). Substitution with PBS increased ADF digestibility and nitrogen retention (p<0.05), but did not affect energy retention. Energy loss as methane ranged between 5.0 and 6.1% of the total gross energy intake. There were no significant differences in carbon dioxide and methane production among all PBS levels, while daily methane production numerically increased with PBS inclusion. Substituting PBS for concentrates did not significantly affect ruminal pH and ammonia N concentration. Total VFA concentration, VFA molar proportions and blood metabolites were also unaffected by PBS replacement. These results suggest that substitution of PBS up to 27% of diet dry matter did not significantly increase methane production and was equal or superior to concentrates in ADF digestibility and nitrogen retention for growing steers.

Bioefficacy of Lysine from L-lysine Sulfate and L-lysine⋅HCl for 10 to 20 kg Pigs

Liu, M.,Qiao, S.Y.,Wang, X.,You, J.M.,Piao, X.S. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.10

The objective of this study was to compare the bioefficacy of L-lysine sulfate relative to L-lysine${\cdot}$HCl for 10 to 20 kg pigs. Two experiments were conducted to determine the bioefficacy of the two sources of lysine using daily gain, feed conversion, plasma urea nitrogen and nitrogen retention as the response criteria. In experiment 1, 168 crossbred barrows ($Landrace{\times}Large$ White), weaned at $28{\pm}3$ d ($9.07{\pm}0.78$kg body weight), were allotted to one of seven dietary treatments in a $2{\times}3$ (two lysine $sources{\times}three $ lysine levels) factorial arrangement of treatments with an added negative control treatment group. The basal diet was based on corn, peanut meal and soybean meal and provided 0.67% lysine. The basal diet was supplemented with 0.1, 0.2 or 0.3% lysine equivalents supplied from either L-lysine sulfate or L-lysine${\cdot}$HCl. Each treatment was fed to six pens of pigs with four pigs per pen. The trial lasted 21 days. The relative bioefficacy value of lysine in L-lysine sulfate using daily gain, feed conversion and plasma urea nitrogen as response criteria was 1.01, 1.05 and 1.04 of the lysine in L-lysine${\cdot}$HCl, respectively. In experiment 2, 42 crossbred ($Landrace{\times}Large$ White) pigs ($16.03{\pm}1.58$ kg body weight) were housed in stainless steel metabolism cages for 10 d and fed the seven diets used in the nitrogen-balance trial. The relative bioefficacy value of L-lysine sulfate was estimated to be 0.95 as effective as L-lysine${\cdot}$HCl for nitrogen retention on an equimolar basis. The t-test analysis revealed that bioefficacy of lysine in L-lysine sulfate was not significantly different from lysine in L-lysine${\cdot}$HCl, which was set at 1.00. In conclusion, L-lysine sulfate can be used instead of L-lysine${\cdot}$HCl to fortify lysine-deficient diets fed to 10 to 20 kg pigs.

RO 농축수 처리를 위한 SBR 공정 적용에 관한 연구

김일회(Il Whee Kim),주현종(Hyun Jong Joo) 大韓環境工學會 2012 대한환경공학회지 Vol.34 No.2

본 연구는 역삼투공정에서 발생하는 농축수의 생물학적 처리에 관한 것으로, SBR공정의 처리성능 및 부하변동과 온 도변화에 따른 효율을 평가하였다. SBR공정의 cycle당 HRT가 각각 8시간과 12시간으로 2가지 형태로 공정운전이 이루어졌으 며, RO농축수의 낮은 C/N비 때문에 효과적인 탈질을 위해 메탄올을 주입하였다. 유량과 온도의 변화에도 SBR공정의 질소제 거 효율은 비교적 안정적인 것으로 나타났다. SBR공정의 최적 time cycle은 2 cycle/day이지만, 3 cycle/day 조건에서도 방류 수 TN 농도가 수질기준 이하로 나타났다. 평가결과 RO 농축수 처리를 위한 SBR공정의 적용은 효과적이었으며, 폐수처리장 설계에 활용이 가능할 것이다. SNR과 SDNR은 각각 0.043~0.066 kg NH₃-N/kg MLVSS·day와 0.096~0.287 kg NO₃ --N/kg MLVSS·day로 나타났다. 도출된 동역학적인자는 RO농축수 처리에서 포기조와 무산소조 설계에 적용이 가능할 것이다. In this study, Application of sequencing batch reactor (SBR) process for RO retentate treatment was performed. Efficiency of treatment by load and temperature variation was tested. The SBR process was operated two types as HRT per one cycle was 8 and 12 hours, respectively. Methanol was injected for an effective denitrificaion owing to low C/N ratio of the RO retentate. TN removal efficiency of the SBR process was relatively stable at the change of flow-rate and temperature. The optimum time cycle of SBR process was 2 cycle/day for TN removal, and in the case of 3 cycle/day, the effluent TN concentration was found under the effluent quality standard. In the result of assessment, the application of SBR process for RO retentate treatment was effective and could be utilized to design for the wastewater treatment plant. The specific nitrification rate (SNR) and specific denitrification rate (SDNR) were 0.043~0.066 kg NH₃-N/kg MLVSS·day and 0.096-0.287 kg NO₃ --N/kg MLVSS·day, respectively. The derived kinetic could be applied for design to the aerobic and anoxic tank in the RO retentate treatment.

RO 농축수 처리를 위한 SBR과 MLE 공정의 비교 평가

이상일,김일회 대한상하수도학회 2011 상하수도학회지 Vol.25 No.6

In this study, the SBR and MLE process was performed for a removal of the RO retentate and the nitrogen removal efficiency was evaluated. The inflow-rate of two processes was set a 10 L/day. The SBR process was operated a two cycle as HRT per one cycle was 12hr and the HRT of the anoxic and aerobic tank was respectively 7.5 hr and 16.5 hr. The methanol was injected for an effective denitrificaion owing to a low C/N ratio of the RO retentate. The two processes were effectively performed for nitrogen removal, but the average removal efficiency of the SBR process was about 94.93% better performance than the MLE process. Therefore, the SBR process demonstrated a good performance more than the MLE process for nitrogen removal of the RO retentate. The kinetic of SNR and SDNR was observed respectively 0.051 kg NH3-N/kg MLVSS·day and 0.287 kg NO3--N/kg MLVSS·day, which will be useful to design for the wastewater treatment system with a RO retentate.