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Relationships among Behavior, Physiological States and Body Weight Gain in Grazing Holstein Heifers
Hasegawa, N.,Hidari, H. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.6
This study examined the behavior of dairy heifers and the factors affecting the performance of them on pasture. Behavior of 10 Holstein heifers in a herd of 25 animals that rotationally grazed five 8-ha pastures was observed and recorded every 5 minutes during 24 hours; body weights were measured once a month from June to October. Blood and rumen fluid samples were collected from 5 of them bimonthly. Chemical composition was analyzed for the forage samples collected each month. CP content (DM basis) of herbage ranged from 12.2 (June) to 17.2% (October) and ADF from 31.1 (October) to 39.1% (July). Standing (posture) time was different significantly among months (p<0.001) ranging from 48.3 to 61.3% of 24 hours and was longer in July and August (61.3% and 58.3%, respectively) when ADF content of herbage was higher than in the other months. Grazing time which significantly differed among months (p<0.001) ranged from 29.1 to 41.6% of 24 hours and was shorter in June and September (29.1% and 33.0%, respectively) when ADF content was lower than in the other months. Average DG through the experiment period was 0.74 kg/day. August was the lowest in DG (0.41 kg/day) and the longest in rumination time and standing-rumination time among months. Animals of higher DG had a shorter standing time (r=-0.36, p<0.01) and a longer lying-rumination time (r=0.55, p<0.001) throughout the experiment. Total protein concentration in blood ranged from 9.04 to 9.64 g/dl and was negatively correlated with DG (r=-0.65, p<0.05). Phospholipid concentration of blood ranged from 119.66 to 156.40 mg/dl and was negatively correlated with DG (r=-0.57, p<0.05). VFA in rumen fluid, acetic acid proportion (ranging from 69.35 to 74.76%) and butyric acid proportion (ranging from 7.18 to 12.05%) showed significant differences among months (p<0.05, p<0.001, respectively). Butyric acid proportion was significantly related with DG (r=0.60, p<0.05).
Vega, R.A.,Hidari, H.,Kuwayama, H.,Suzuki, M.,Manalo, D.D. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3
Six 16 months old Holstein steers were offered ad libitum feed for 7 months, to determine the (1) relationships of backfat thickness (BFT) to plasma leptin, and insulin; and (2) associations of TDN intake/kg body weight (BW) to plasma leptin, BFT and insulin. Feed intake, body weight and BFT were measured on selected monthly ages from day 1 to 8, day 1 and 8, and day 8, respectively. Blood was sampled on day 8 and the plasma was analyzed for leptin, insulin, glucose, NEFA, total cholesterol and triglyceride. Body weight and BFT increased, while TDN intake per kg BW decreased from 16 to 23 months old. Plasma leptin increased and mimicked the level of insulin, resulting to significant correlation (r=0.54; p<0.002). TDN intake was negatively related to plasma leptin (r=0.49; p<0.004), insulin (r=0.41; p<0.02) and BFT at 12 to 13th rib (r=0.48; p<0.005). Backfat thickness at 12 to 13th rib was positively related to plasma leptin (r=0.45; p<0.01). Negative associations of TDN intake with plasma leptin and BFT during finishing period suggest long-term involvement of adipose tissues in the feed intake regulation of steers fed high concentrate diet.
Vega, R.A.,Hidari, H.,Matsunaga, N.,Kuwayama, H.,Manalo, D.D.,Lee, H.G.,Hata, H. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7
In a herd of 24 spring-born steers, plasma leptin and performance of selected purebred (n=5) and backcrossed Hereford (n=5) were compared in a year-round summer grazing and winter feedlot fattening. Bimonthly blood collection and body weight measurement were accomplished. The plasma samples were analyzed for leptin, insulin, total cholesterol, triglyceride, NEFA and glucose. The experimental design utilized one-way ANOVA with breed as the treatment. The purebred obtained higher plasma NEFA (p<0.001) compared to backcross, regardless of seasonal feeding systems (SFS). The backcross showed gradual increase and nonresponsiveness of plasma leptin to SFS. During summer grazing, attenuation of plasma leptin and sudden elevation when shifted to winter feedlot fattening were observed in purebred. Plasma leptin obtained linear relationship with body weight of purebred (r=0.53;p<0.001) and backcrossed Hereford (r=0.49; p<0.01). The purebred and backcrossed Hereford, when shifted to summer grazing, resulted to sustained and restricted daily gain, respectively. Therefore, cattle breeds of higher growth potential exhibit significant elevation of plasma leptin after 400 kg BW, when animal starts to deposit significant body fat.
Lee, H.G.,Hidari, H.,Kang, S.K.,Hong, Z.S.,Xu, C.X.,Kim, S.H.,Seo, K.S.,Yoon, D.H.,Choi, Y.J. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11
This study was conducted to determine the characteristics of IGFBPs in plasma of steers, and to profile the relationship between growth and plasma IGF-1 and IGFBPs with aging in Holstein steers. Four blots of IGFBP at molecular weights of 38-43, 34, 29-32 and 24 kDa bands were detected by western ligand blot assay using $^{125}I-IGF-1$. On the basis of immunoblotting with anti-bovine IGFBP-2 and -3 antiserums, we observed the band for IGFBP-2 at approximately 34 kDa, and the IGFBP-3 band was detected at 38-43 kDa and 34 kDa in adult steers and calves. The IGFBP-3 antiserum used on the blots exhibited significant cross-reactivity with 34 kDa IGFBP-2. Furthermore, the 38-43 kDa IGFBP-3 bands were reduced to a 36 kDa band after deglycosylation, whereas the 34 kDa IGFBP-2 was intact. The plasma IGF-1, IGFBP-3 and other IGFBPs showed stability throughout a whole day. The change in live weight was found to be positively correlated to the plasma IGF-1 concentration (r = 0.6801, n = 64, p<0.05) and plasma IGFBP-3 (r = 0.6321, n = 64, p<0.05), while inversely correlated to plasma IGFBP-2 (r = -0.2919, n = 64, p<0.05). Furthermore, plasma IGF-1 was positively correlated to plasma IGFBP-3 (r = 0.6191, p<0.001), but was not correlated to plasma IGFBP-2. The portion of IGFBP-2 for total IGFBPs in calves was higher than in adult steers (p<0.05) and was decreased with growth, whereas that of IGFBP-3 was increased with increased live weight (p<0.05). The ratio IGFBP-3 for IGFBP-2 (BP-3/BP-2) was increased with growing of liveweight. Therefore, the changes in plasma IGF-1 level with increased liveweight may be related to the changes in plasma IGFBP-3 level and IGFBP-2 may give an important role in anabolic action of IGF-1 with the growth of body during calfhood in Holstein steers.
Pen, B.,Oyabu, T.,Hidaka, S.,Hidari, H. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.4
Ten 18.5-month old Holstein steers were allocated into two diet groups of five and fed either concentrates as control group or potato by-products based silage (PBS) as experimental group for six months to investigate the effect of PBS on growth performance, carcass characteristics and fatty acid composition of carcass fats. The PBS diet consisted, in a DM basis, of 74.5% PBS, 16% hay, and 9.5% soybean milk residue (SMR). The control diet consisted, in a DM basis, of 82.5% concentrates and 17.5% hay. There were no significant differences in the overall average daily gain (ADG), dry matter intake (DMI), feed efficiency, yield score and meat quality score of dressed carcass between the two diet groups. There were no statistical differences in DM, crude protein (CP), and ether extract (EE) of beef and melting point of all adipose tissues from steers fed both diets. The PBS-fed steers tended to have lower Warner Bratzler shear (WBS) values than in the concentrate-fed steers (13.0 vs. 17.7 lb, p<0.1). At both sampling times 3 and 6 months of feeding period, PBS-fed steers had higher proportions of polyunsaturated fatty acids (PUFA), especially linoleic (C18:2) acids in subcutaneous fat than in control group (p<0.05). PBS-fed steers had significantly higher proportions of PUFA, especially C18:2 fatty acids than in concentrate-fed steers in carcass fats (p<0.05). In conclusion, feeding PBS to fattening steers has shown to have the same potential as concentrate feeding in terms of effect on the growth performance, feed efficiency, and carcass characteristics. Interestingly, PBS also seems preferable to concentrates because it increased the proportion of C18:2 fatty acid composition of carcass fats which is valuable for beef consumers.