간손상 유발실험동물에서 Toluene의 피부독성

채순님,이상희,윤종국,이상일,조현국 한국환경보건학회 2001 한국환경보건학회지 Vol.27 No.2

To evaluate the cutaneous injury in liver damaged rats by toluene application to the skin, toluene(35mg/㎤) was sequentially applied for 5 days to the dorsal skin of liver damaged rats with CCl4 (6 times ever other day:0.1ml/100 g body weight-50% CCl4in olive oil). The cutaneous ultrastructural changes were unexoectably not observed in liver from CCl4-treated rats although necrotic liver damage appeared under light microscope. In these animals by the application of toluene to rat skin the cutaneous xanthine oxidase activity was significantly increased(p<0.05), but cytochrome P450 content was not different from that of the control or only CCl4-treated rats. On the other hand, the cutaneous superoxide dismutase and catalase activities in liver damaged animals were significantly respectively(p<0.05, p<0.001), decreased by toluene application to the skin compared with control and especially the former enzyme activity was significantty decreased(p<0.01), compared with that of liver damaged rate rat but glutathione peroxidase, glutathione-S-transferase activities were not significantly different from those of the control or liver damaged rats. Futhermore, the reduced gluathione content of skin was also significantly decreased by toluene application to the liver damaged animals. In conclusion, the great deposits of cerrous peroxide and ultramorphological changes in skin tissue of liver damaged animals by toluene application may be responsible for the oxygen free radical.

Effect of Toluene Application to Skin on the Liver Injury in Rats

Chae, Soon-Nim,Lee, Sang-Hee,Yoon, Chong-Guk 대한의생명과학회 2001 Journal of biomedical laboratory sciences Vol.7 No.1

To investigate an effect of the topical toluene application to rat skin on the liver injury, toluene (35 mg/cm2) was sequentially applied for 3 or 5 days to rat skin and then the animals were sacrificed. 5 day toluene-treated rats showed the slight increase of liver weight per body weight (%) compared with control. Serum levels of xanthine oxidase and alanine aminotransferase activity were significantly increased both in 3 days and 5 days toluene-treated animals compared with control. In the histopathological findings, cytoplasmic degeneration of hepatocytes around the central vein was noted in the liver of rats applied with toluene to the skin. These results indicate toluene application to rat shin lads to somewhat slight liver injury. On the other hand, the hepatic benzylalcohol or aldehyde dehydrogenase acclivities were significantly decreased by toluene application to rat skin. In conclusion the liver injury was induced by toluene application to rat skin and it can be hypothesized that accumulation of benzaldehyde in liver cell may be responsible for liver injury.

Study on the Radiation Dose about Skin Thickness of Rat

Hongmoon Jung(정홍문),Doyeon Won(원도연),Hyeongyun Kim(김형균),Jaeeun Jung(정재은),hyeun-woo Choi(최현우) 한국방사선학회 2016 한국방사선학회 논문지 Vol.10 No.6

방사선장해 극복을 위한 조직공학실험에서는 다양한 실험방법이 존재한다. 그중에서도 방사선 장해모델을 구현함에 있어서 쥐는 가장 많이 사용되는 실험동물이다. 이번 실험에서는 쥐 피부를 벗긴 후 일정한두께로 만든 후에 피부 두께에 따른 방사선량을 측정하였다. 또한 쥐의 피부 두께에 따른 방사선 흡수선량을 유추해 날수 있는 수식을 도식화 하였다. 결론적으로 이러한 수식을 적용한다면 방사선장해극복을 위한 조직공학 연구에서 사용하는 다양한 지지체삽입시에 지지체가 받을 수 있는 방사선량의 정도를 유추해낼 수 있으며 이것을 통하여 쥐 피부두께 피하에 관한 체내 (in-vitro)실험모델 구현 시 방사선량의 정보를 제공하고자 한다. 따라서 이번 연구의 결과를 기반으로 사용한다면 방사선이 노출된 쥐의 피하조직 체내 (in-vitro) 조직공학 실험에서 효과적으로 사용 될 수 있을 것이다. A rat is the most common experimental animal used for the realization of the radiation injury model. The certain thickness of rat skin was prepared by peeling off a rat skin. Radiation level was measured by using this rat skin. Also, The schematic of the formula was made that can predict the radiation absorbed dose (RAD) as a function of the thickness of the rat skin. Consequently, we will provide the RAD information in the realization of in-vitro experimental model regarding the rat’s skin thickness by applying the formulas. Moreover, the results from this study can be effectively used for the in-vitro experiment of the rat subcutaneous tissue which was exposed to radiation.

흰쥐의 피부조직에 있어서 Cyclohexane의 독성

전태원,조현국,윤종국 한국환경보건학회 2002 한국환경보건학회지 Vol.28 No.2

To evaluate the skin toxicity of topical cyclohexane application (25mg/cm2) was sequentially applied to the rat skin for four days. On the histopathological findings in the light micrographs, neutrophils and engulfed neutrophils are seen, and many cytoplasmic processes were appeared in proliferated layer whereas in the dermis area, increased numbers of fibroblast, accumulation of neutrophil and lipid droplets are demonstrated. On the other hand, applying the cyclohexane to the rat skin led to the remarkable rise of cutaneous xanthine oxidase activity and similar activities of superoxide dismutase and glutathione peroxidase and glutathione content and declined activity of glutathione S-transferase compared with control group. Especially the remarkably decreased activity of aniline hydroxylase (AH) was appeared in skin as little as scarcely determined. Furthermore, the applying the cyclohexane to skin led to the significantly increased activity of hepatic AH and alcohol dehydrogenase. These results indicate that oxygen free radical and intermediate metabolite of cyclohexane may be responsible for structural changes in skin by cyclohexane application to rat skin.

Effect of Toluene Application to Skin on the Liver Injury in Rats

Soon-Nim Chae,Sang-Hee Lee,Chong-Guk Yoon 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.1

To investigate an effect of the topical toluene application to rat skin on the liver injury, toluene (35 ㎎/㎠) was sequentially applied for 3 or 5 days to rat skin and then the animals were sacrificed. 5 day toluene-treated rats showed the slight increase of liver weight per body weight (%) compared with control. Serum levels of xanthine oxidase and alanine aminotransferase activity were significantly increased both in 3 days and 5 days toluene-treated animals compared with control. In the histopathological findings, cytoplasmic degeneration of hepatocytes around the central vein was noted in the liver of rats applied with toluene to the skin. These results indicate toluene application to rat skin leds to somewhat slight liver injury. On the other hand, the hepatic benzylalcohol or aldehyde dehydrogenase activities were significantly decreased by toluene application to rat skin. In conclusion, the liver injury was induced by toluene application to rat skin, and it can be hypothesized that accumulation of benzaldehyde in liver cell may be responsible for liver injury.

Grape skin improves antioxidant capacity in rats fed a high fat diet

Su-Jin Lee,Soo-Kyong Choi,Jung-Sook Seo 한국영양학회 2009 Nutrition Research and Practice Vol.3 No.4

This study was conducted to investigate the effect of dietary grape skin on lipid peroxidation and antioxidant defense system in rats fed high fat diet. The Sprague-Dawley rats were fed either control (5% fat) diet or high fat (25% fat) diet which was based on AIN-93 diet for 2 weeks, and then they were grouped as control group (C), control + 5% grape skin group (CS), high-fat group (HF), high fat + 5% grape skin group (HFS) with 10 rats each and fed corresponding diets for 4 weeks. The hepatic thiobarbituric acid reacting substances (TBARS) were increased in high fat group as compared with control group, but reduced by grape skin. The serum total antioxidant status, and activities of hepatic catalase and superoxide dismutase, xanthine oxidase and glucose-6-phosphatase were increased by supplementation of grape skin. Glutathione peroxidase activity was significantly higher in CS group than in C group. Grape skin feeding tended to increase the concentration of total glutathione, especially in control group. The ratio of reduced glutathione to oxidized glutathione was lower in high fat groups than in control groups. The ratio was increased by dietary supplementation of grape skin in control group. These results suggest that dietary supplementation of grape skin would be effective on protection of oxidative damage by lipid peroxidation through improvement of antioxidant defense system in rats fed high fat diet as well as rats with low fat diet.

Grape skin improves antioxidant capacity in rats fed a high fat diet

Lee, Su-Jin,Choi, Soo-Kyong,Seo, Jung-Sook The Korean Nutrition Society 2009 Nutrition Research and Practice Vol.2 No.3

This study was conducted to investigate the effect of dietary grape skin on lipid peroxidation and antioxidant defense system in rats fed high fat diet. The Sprague-Dawley rats were fed either control (5% fat) diet or high fat (25% fat) diet which was based on AIN-93 diet for 2 weeks, and then they were grouped as control group (C), control + 5% grape skin group (CS), high-fat group (HF), high fat + 5% grape skin group (HFS) with 10 rats each and fed corresponding diets for 4 weeks. The hepatic thiobarbituric acid reacting substances (TBARS) were increased in high fat group as compared with control group, but reduced by grape skin. The serum total antioxidant status, and activities of hepatic catalase and superoxide dismutase, xanthine oxidase and glucose-6-phosphatase were increased by supplementation of grape skin. Glutathione peroxidase activity was significantly higher in CS group than in C group. Grape skin feeding tended to increase the concentration of total glutathione, especially in control group. The ratio of reduced glutathione to oxidized glutathione was lower in high fat groups than in control groups. The ratio was increased by dietary supplementation of grape skin in control group. These results suggest that dietary supplementation of grape skin would be effective on protection of oxidative damage by lipid peroxidation through improvement of antioxidant defense system in rats fed high fat diet as well as rats with low fat diet.

스트렙토조토신 유도 당뇨 흰쥐에서 전기자극이 상처치유와 피부 비만세포에 미치는 영향

제갈승주 ( Seung Joo Jekal ),이경선 ( Kyung Sun Lee ),정옥봉 ( Ok Bong Chung ),이재형 ( Jae Hyoung Lee ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2

The aim of this study was to investigate the effect of electrical stimulation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Thirty male Sprague-Dawley rats were divided into three groups : incision (control), diabetes+incision (diabetes) and diabetes + incision + electrical stimulation (D/ES). Diabetes was induced in rats by streptozotocin (STZ) injection (60 mg/kg, one time) and 20 mm length incision wounds were created on the back after shaving hair. The electrical stimulation rats were treated with a current intensity of 30~50 V at 120 pps and 140 μs for 10 days from 3 days after STZ injection. The lesion and adjacent skin tissues were fixed with 10% buffered formalin, embedded with paraffin. For wound healing analysis, hematoxylin-eosin (HE) and picrosirius red staining were performed. Mast cells (MC) were stained with toluidine blue (pH 0.5) and quantified at ×200 using a light microscope. The density of keratinocyte proliferation and microvessels in skin tissues were analyzed using a computerized image analysis system on sections immunostained with proliferative cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA), respectively. The results showed that the wound healing rate, collagen density and neoepidermis thickness, density of PCNA-positive cells and density of α-SMA-positive vessels were significantly higher in D/ES rats than in diabetic rats. The density of MCs and degranulated MCs in D/ES rats were also significantly higher than those in diabetic rats. These findings suggest that the electrical stimulation may promote the tissue repair process by accelerating collagen production, keratinocyte proliferation and angiogenesis in the diabetic rats, and MCs are required for wound healing of skin in rats.

The Activity of Xanthine Oxidase(Type O) in Some Partial Portions of Rat Skin

Lee, Sang-Hee,Yoon, Chong-Guk 대한의생명과학회 2002 Biomedical Science Letters Vol.8 No.3

To evaluate the physiological significance of xanthine oxidase (XO) in rat skin, the activity of XO (type O) in skin was compared with that of small intestine or liver. Concomitantly, XO activities in some partial portions (scalp, leg, dorsal and ventral part) of skin were detemined and then compared with each partial portion. XO activity of skin was lover than that of small intestine and rather higher than that of liver. Furthemore, the activity of XO in skin, after clipping of hairs and then in 5 days, was more increased than that of rat which was clipped before having been sacrificed. As for activities of free radical scavenging system (GPx, GST, SOD), skin is lower than liver and small intestine. Although it is known that the oxygen free radical generated by XO system lead to injurious effect on the cell, the XO activity of ventral part which is to be less exposed to xenobiotics and biological agents was the lowest among those of ventral, dorsal, leg and scalp parts in skin. In conclusion, it may be hypothesized that XO system in skim act on defence mechanism.

The Activity of Xanthine Oxidase (Type O) in Some Partial Portions of Rat Skin

Lee, Sang-Hee,Yoon, Chong-Guk 대한의생명과학회 2002 Journal of biomedical laboratory sciences Vol.8 No.3

To evaluate the physiological significance of xanthine oxidase (XO) in rat skin, the activity of XO (type O) in skin was compared with that of small intestine or liver. Concomitantly, XO activities in some partial portions (scalp, leg, dorsal and ventral part) of skin were determined and then compared with each partial portion. XO activity of skin was lower than that of small intestine and rather higher than that of liver. Furthermore, the activity of XO in skin, after clipping of hairs and then in 5 days, was more increased than that of rat which was clipped before having been sacrificed. As for activities of free radical scavenging system (GPx, GST, SOD), skin is lower than liver and small intestine. Although it is hewn that the oxygen free radical generated by XO system lead to injurious effect on the cell, the XO activity of ventral part which is to be less exposed to xenobiotics and biological agents was the lowest among those of ventral, dorsal, leg and scalp parts in skin. In conclusion, it may be hypothesized that XO system in skin act on defence mechanism.