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Jeon, Se Jin,Park, Ho Jae,Gao, Qingtao,Lee, Hyung Eun,Park, Se Jin,Hong, Eunyoung,Jang, Dae Sik,Shin, Chan Young,Cheong, Jae Hoon,Ryu, Jong Hoon Elsevier 2015 Behavioural brain research Vol.291 No.-
<P><B>Abstract</B></P> <P>Sleep loss, insomnia, is considered a sign of imbalance of physiological rhythm, which can be used as pre-clinic diagnosis of various neuropsychiatric disorders. The aim of the present study is to understand the pharmacological actions of α- or β-amyrin, natural triterpene compound, on the sleep in mice. To analyze the sleeping behavior, we used the well-known pentobarbital-induced sleeping model after single administration of either α- or β-amyrin. The sleeping onset time was remarkably decreased and duration was prolonged by β-amyrin (1, 3, or 10mg/kg) but not by α-amyrin (1, 3, or 10mg/kg). These effects were significantly blocked by GABA<SUB>A</SUB> receptor antagonist, bicuculline. Moreover, β-amyrin increased brain GABA level compared to the vehicle administration. Overall, the present study suggests that β-amyrin would enhance the total sleeping behavior in pentobarbital-induced sleeping model via the activation of GABAergic neurotransmitter system through GABA content in the brain.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pentobarbital-induced sleeping behavior was prolonged by β-amyrin. </LI> <LI> Increased sleeping behavior was blocked by GABA<SUB>A</SUB> receptor antagonist. </LI> <LI> Brain GABA level was increased by β-amyrin administration. </LI> </UL> </P>
Seo, Dae-Gun,Kim, Sunggun,Lee, Da Kyung,Kim, Na Yeon,Lee, Jae-Sun,Hwang, Kwang Woo,Park, So-Young Elsevier 2019 Phytomedicine Vol.63 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>T helper 17 (Th17) cells, which are differentiated from CD4<SUP>+</SUP> T cells, drive inflammation, leading to autoimmune diseases such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease. Therefore, inhibiting Th17 polarization could be a therapeutic target for inflammatory diseases.</P> <P><B>Purpose</B></P> <P>We investigated the inhibitory effect of <I>Fraxinus rhynchophylla</I> (Oleaceae) on Th17 differentiation and found its active component.</P> <P><B>Study design</B></P> <P>The activity of <I>F. rhynchophylla</I> and its active constituent was verified using CD4<SUP>+</SUP> cells extracted from C57BL/6 mice.</P> <P><B>Methods</B></P> <P>Micro-environment for Th17 polarization was provided to CD4<SUP>+</SUP> cells and the effect of treatment with samples was measured by enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and Western blot.</P> <P><B>Results</B></P> <P>The extract of <I>F. rhynchophylla</I> Hance and its chemical constituent, α-amyrin acetate, which was isolated via bioassay-guided isolation, significantly inhibited Th17 polarization as revealed when interleukin (IL)-17, a characteristic cytokine produced by Th17 cells, was measured. Furthermore, the inhibitory effect of α-amyrin acetate was compared to the amyrin derivatives, α-amyrin and β-amyrin. All displayed a suppressive effect on Th17 polarization and all reduced the expression of single transducer and activator of transcription 3 (STAT3) and retinoic acid receptor-related orphan receptor γt (RORγt), which are crucial transcription factors regulating Th17 differentiation. α-Amyrin acetate, however, exhibited the most prominent effects, which indicates that the functional group, acetate, might strengthen the inhibitory effect on Th17 differentiation.</P> <P><B>Conclusion</B></P> <P>Taken together, these results suggest that the extract of <I>F. rhynchophylla</I> and its active constituent, α-amyrin acetate, could be applied as a potential therapeutic agent for autoimmune disorders such as rheumatoid arthritis.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Chenniappan KUPPUSAMY,Kadarkarai MURUGAN,Narayanasamy ARUL,Perumal YASODHA 한국곤충학회 2009 Entomological Research Vol.39 No.1
Vector control is a serious concern in developing countries. Over the past two decades, phytochemicals have received progressively more attention as insecticide alternatives, and they have recently become the focus in the concept of integrated vector control. α-Amyrin acetate, the n-hexane fraction of acetone extract from the leaves of Catharanthus roseus, was evaluated for its larvicidal, pupicidal and fecundity effects as well as insect growth regulator activity against the malaria vector Anopheles stephensi Liston. The highest concentration of 1 p.p.m. produced 100% mortality in first to second instars and 94% mortality in third and fourth instars. In addition, the duration of larval instars and the total developmental time were prolonged, while female longevity and fecundity were markedly decreased. The suppression of pupation and adult emergence was probably due to its action similar to juvenile hormone analogs in combination with growth regulator activity and toxicity, which reduced the overall performance of the malaria vector An. stephensi.
산복사나무(Prunus davidiana) 열매로부터 Triterpenoid의 분리 및 동정
이민지 ( Min Jee Lee ),김지혜 ( Ji Hye Kim ),차병주 ( Byeong Ju Cha ),서경화 ( Kyeong Hwa Seo ),백남인 ( Nam In Baek ),이윤형 ( Youn Hyung Lee ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.2
산복사나무(Prunus davidiana) 열매를 실온에서 80% MeOH수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, 물 분획으로 나누었다. EtOAc 분획에 대하여 SiO2과 ODScolumn chromatography를 반복 실시하여 3종의 화합물을 분리 및 정제하였다. NMR, MS, 및 IR data 등을 해석하여, ursolicacid (1), corosolic acid (2), 그리고 α-amyrin (3)로 구조동정하였다. The fruits of Prunus davidiana were extracted with 80 % aqueous methanol at room temperature. The concentrated extract was partitioned as ethyl acetate (EtOAc), n-butyl alcohol, and water fractions. From the EtOAc fraction, three triterpenoids were isolated through the repeated silica gel (SiO2) and octadecyl SiO2 column chromatographies. Based on physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared, the compounds were identified to be ursolic acid (1), corosolic acid (2), and α-amyrin (3).
천연물,유기합성 : 식용 식물자원으로부터 활성물질의 탐색 8 -개망초(Erigeron annuus L.) 꽃으로부터 triterpenoid의 분리-
김동현 ( Dong Hyun Kim ),정성제 ( Sung Je Jung ),방면호 ( Myun Ho Bang ),정인식 ( In Sik Chung ),김성훈 ( Sung Hoon Kim ),권병목 ( Byoung Mog Kwon ),김대근 ( Dae Keun Kim ),박미현 ( Mi Hyun Park ),백남인 ( Nam In Baek ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.4