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- 저자 : zhiyi zuo (검색결과 6 건)
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Cloning and Distribution of Facilitative Glucose Transporter 2 (SLC2A2) in Pigs
Zuo, Jianjun,Huang, Zhiyi,Zhi, Aimin,Zou, Shigeng,Zhou, Xiangyan,Dai, Fawen,Ye, Hui,Feng, Dingyuan Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9
Glucose is the main energy source for mammalian cells and its absorption is co-mediated by two different families of glucose transporters, sodium/glucose co-transporters (SGLTs) and facilitative glucose transporters (GLUTs). Here, we report the cloning and tissue distribution of porcine GLUT2. The GLUT2 was cloned by RACE and its cDNA was 2,051 bp long (GenBank accession no. EF140874). An AAATAA consensus sequence at nucleotide positions 1936-1941 was located upstream of the poly $(A)^+$ tail. Open reading frame analysis suggested that porcine GLUT2 contained 524 amino acids, with molecular weight of 57 kDa. The amino acid sequence of porcine GLUT2 was 87% and 79.4% identical with human and mouse GLUT2, respectively. GLUT2 mRNA was detected at highest level in porcine liver, at moderate levels in the small intestine and kidney, and at low levels in the brain, lung, muscle and heart. In the small intestine, the highest level was in the jejunum. In conclusion, the mRNA expression of GLUT2 was not only differentially regulated by age, but also differentially distributed along the small intestine of piglets, which may be related to availability of different intestinal luminal substrate concentrations resulting from different food sources and digestibility.
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Yun, Jungyeon,Li, Jun,Zuo, Zhiyi Elsevier 2014 Brain research bulletin Vol.103 No.-
<P>Ischemic preconditioning-induced neuroprotection is a well-known phenomenon. We hypothesize that this form of neuroprotection is transferable among the same type of cells. To test this hypothesis, human neuroblastoma SH-SY5Y cells were induced to become neuron-like cells. Primary rat cortical neuronal cultures were also used. These cells were subjected to various lengths of short oxygen-glucose deprivation (OGD, an in vitro simulation of ischemia) and then 1-h OGD. Some cells that were not exposed to a short episode of ischemia were incubated with culture medium from the cells that had 3- or 5-min OGD. Those cells were subjected to OGD for 1h at 1 or 24h after they were exposed to the medium. Cell injury was evaluated at 24h after the 1-h OGD by lactate dehydrogenase release assay. In another experiment, cells subjected to a 3-min OGD or exposed to the medium from cells that had a 3-min OGD were harvested at 30min after the OGD or the medium exposure for Western blotting of Akt, a prosurvival protein. Our study showed that a prior episode of ischemia lasting from 3 to 10min significantly reduced the 1-h OGD-induced cell injury. Medium from cells subjected to a 3-min OGD also induced acute and delayed phases of neuroprotection in OGD-na?ve human neuron-like cells and primary rat cortical neuronal cultures. Cells subjected to a 3-min OGD or incubated with the medium from cells exposed to a 3-min OGD had increased phosphorylated/activated Akt. The increased phosphorylated Akt and neuroprotection induced by medium transferring were inhibited by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), an adenosine A1 receptor inhibitor. The 3-min OGD-induced neuroprotection was inhibited by LY294002, an Akt activation inhibitor. These results suggest that ischemic preconditioning-induced neuroprotection is transferable among the cells. Small molecules, such as adenosine, may mediate this effect.</P>
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Volatile Anesthetics Attenuate Oxidative Stress-Reduced Activity of Glutamate Transporter Type 3 :
Lee, Soon-Ae,Choi, Jun-Gwon,Zuo, Zhiyi Ovid Technologies (Wolters Kluwer) - AnesthesiaAna 2009 Anesthesia and analgesia Vol.109 No.5
<P>BACKGROUND: Volatile anesthetics enhance the activity of glutamate transporter Type 3 (also called excitatory amino acid transporter Type 3, EAAT3), the major neuronal EAAT. In addition to glutamate, EAAT3 can also uptake L-cysteine, the rate-limiting substrate for the synthesis of glutathione. Our previous study showed that oxidative stress inhibited glutamate-induced EAAT3 activity. We determined whether oxidative stress would reduce L-cysteine-induced EAAT3 activity and whether this reduction would be attenuated by volatile anesthetics. METHODS: Rat EAAT3 was expressed in Xenopus oocytes. L-glutamate- and L-cysteine-induced membrane currents were recorded using the 2-electrode voltage clamp technique. The peak current was quantified to reflect the amount of transported substrates because transport of substrates via EAATs is electrogenic. RESULTS: Exposure of oocytes to 5 mM tert-butyl hydroperoxide, an organic oxidant, for 10 min reduced the V(max), but did not affect the K(m), of EAAT3 for L-cysteine. The volatile anesthetics isoflurane, sevoflurane, and desflurane at concentrations from 1% to 3% attenuated the tert-butyl hydroperoxide-reduced EAAT3 activity for L-glutamate and L-cysteine. CONCLUSIONS: Our results suggest that volatile anesthetics preserve EAAT3 function to transport L-glutamate and L-cysteine under oxidative stress, which may be a mechanism for the neuroprotective effects of volatile anesthetics.</P>
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Effects of Tianeptine on The Activity of Glutamate Transporter EAAT3 Expressed in Xenopus Oocytes
Bon-Wook Koo(구본욱),Hyo-Seok Na(나효석),Jung-Hee Ryu(유정희),Jung-Seok Choi(최정석),Sang-Hwan Do(도상환),Zhiyi Zuo(주오지이) 대한생물치료정신의학회 2020 생물치료정신의학 Vol.26 No.3
배경:티아넵틴(tianeptine)은 최근 주목받고 있는 항우울제로 기존의 모노아민 항우울제(monoaminergic antidepressants)와 달리 케타민(ketamine)과 같이 글루타메이트(glutamate)의 신경전달에 영향을 미치는 것으로 알려져 있다. 글루탐산염 전달체, 특히 흥분성 아미노산 전달체 3형(Excitatory Amino Acid Transporter 3, EAAT3)에 대한 연구가 계속되어 왔지만 티아넵틴이 EAAT3에 미치는 영향에 대한 연구는 부족하다. 방법:개구리(Xenopus laevies) 난모세포에 EAAT3의 mRNA를 세포질내 주입하여 발현시킨 후, 2개의 전극을 이용한 전압클램프를 사용하여 L-glutamate 투여 전, 중, 후로 막전위를 측정하였다. 반응값은 막전위의 변화를 적분하여 구하였으며, microcoulomb 단위로 제시하였다. 데이터는 평균 ± 표준오차로 표기하였다. 결과:티아넵틴은 EAAT3의 활성도를 농도의존적으로(0.1~100μM) 감소시켰으며 1mM 티아넵틴을 72시간 동안 노출 시켰을 때 Km(27.0±7.6 vs. 23.3±4.9mM, n=84, p=0.72)의 변화없이 Vmax(6.9±0.6 vs. 4.8±0.3mC, n=84, p<0.05)를 유의미하게 줄였다. 결론:개구리 난모세포에 티아넵틴을 72시간 동안 노출시켰을 때 농도의존적으로(1~100mM) EAAT3의 활성도를 감소시켰다. 이번 연구의 결과는 티아넵틴이 EAAT3의 이용 가능한 수나 회전률을 감소시킴으로써 활성도를 감소시키는 것을 시사한다. Objectives : Tianeptine is an antidepressant that has drawn attention recently. Unlike traditional monoaminergic antidepressants, tianeptine is known to affect glutamate neurotransmission like ketamine. However, there has been paucity of studies investigating the role of tianeptine on glutamate transporters, especially excitatory amino acid transporter type 3 (EAAT3). Methods : After expression of EAAT3 by intracellular injection of EAAT3 mRNA, we investigated the effect of tianeptine on the activity of EAAT3, by measuring membrane current in response to L-glutamate administration using Xenopus oocyte expression system and two-electrode voltage clamps. Results : Tianeptine (1mM for 72h) significantly reduced Vmax (6.9±0.6 vs. 4.8±0.3mC, n=14-22, p<0.05) without changing Km (27.0±7.6 vs. 23.3±4.9mM, n=14-22, p=0.72). Conclusion : When tianeptine was exposed for 72h, it decreased the activity of EAAT3 in a concentration-dependent manner (1-100mM). Our results suggest that tianeptine decreases EAAT3 activity by reducing the available number or turnover rate of EAAT3.
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