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Removing Lipemia in Serum/Plasma Samples: A Multicenter Study
María-José Castro-Castro,Beatriz Candás-Estébanez,Margarita Esteban-Salán,Pilar Calmarza,Teresa Arrobas-Velilla,Carlos Romero-Román,Miguel Pocoví-Mieras,José-Ángel Aguilar-Doreste,Sociedad Española de 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.6
Background: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. Methods: Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods—LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). Results: When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
THE EVOLUTION OF ASTRONOMICAL OBSERVATORY DESIGN
Miguel Ángel Castro Tirado,Alberto J. Castro-Tirado 한국천문학회 2019 Journal of The Korean Astronomical Society Vol.52 No.4
This work addresses the development of the astronomical observatory all through history, from an architectural point of view, as a building in relation to the observing instruments and their functioning as a heterogeneous work center. We focused on 32 observatories (in the period 1259–2007) and carefully analyzed the architectures. Considering the impact of the construction itself or its facilities on the results of the research (thermal or structural stability, poor weather protection, turbulence, etc.), there is little attention paid to theories or studies of the architectural or construction aspects of the observatories. Therefore, this work aims to present a theoretical-critical contribution that, at least, invites the reflection of those involved in the development of astronomical observatories in the future.
Gustavo Batista de Castro Souza,Flávio Henrique Teles Vieira,Cláudio Ribeiro Lima,Getúlio Antero de Deus Júnior,Marcelo Stehling de Castro,Sérgio Granato de Araujo,Thiago Lara Vasques 한국전자통신연구원 2016 ETRI Journal Vol.38 No.1
Smart metering systems have become widespread around the world. RF mesh communication systems have contributed to the creation of smarter and more reliable power systems. This paper presents an algorithm for positioning GPRS concentrators to attain delay constraints for a ZigBee-based mesh network. The proposed algorithm determines the number and placement of concentrators using integer linear programming and a queueing model for the given mesh network. The solutions given by the proposed algorithm are validated by verifying the communication network performance through simulations.
Castro-Perez, B.I.,Garzon-Proano, J.S.,Lopez-Soto, M.A.,Barreras, A.,Gonzalez, V.M.,Plascencia, Alejandro,Estrada-Angulo, A.,Davila-Ramos, H.,Rios-Rincon, F.G.,Zinn, R.A. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8
Four male lambs (Katahdin; average live weight $25.9{\pm}2.9$ kg) with "T" type cannulas in the rumen and proximal duodenum were used in a $4{\times}4$ Latin square experiment to evaluate the influence of supplemental dry distillers grain with solubles (DDGS) levels (0, 10, 20 and 30%, dry matter basis) in substitution for dry-rolled (DR) corn on characteristics of digestive function and digestible energy (DE) of diet. Treatments did not influence ruminal pH. Substitution of DR corn with DDGS increased ruminal neutral detergent fiber (NDF) digestion (quadratic effect, p<0.01), but decreased ruminal organic matter (OM) digestion (linear effect, p<0.01). Replacing corn with DDGS increased (linear, $p{\leq}0.02$) duodenal flow of lipids, NDF and feed N. But there were no treatment effects on flow to the small intestine of microbial nitrogen (MN) or microbial N efficiency. The estimated UIP value of DDGS was 44%. Postruminal digestion of OM, starch, lipids and nitrogen (N) were not affected by treatments. Total tract digestion of N increased (linear, p = 0.04) as the DDGS level increased, but DDGS substitution tended to decrease total tract digestion of OM (p = 0.06) and digestion of gross energy (p = 0.08). However, it did not affect the dietary digestible energy (DE, MJ/kg), reflecting the greater gross energy content of DDGS versus DR corn in the replacements. The comparative DE value of DDGS may be considered similar to the DE value of the DR corn it replaced up to 30% in the finishing diets fed to lambs.
SOME ASPECTS OF ZARISKI TOPOLOGY FOR MULTIPLICATION MODULES AND THEIR ATTACHED FRAMES AND QUANTALES
Castro, Jaime,Rios, Jose,Tapia, Gustavo Korean Mathematical Society 2019 대한수학회지 Vol.56 No.5
For a multiplication R-module M we consider the Zariski topology in the set Spec (M) of prime submodules of M. We investigate the relationship between the algebraic properties of the submodules of M and the topological properties of some subspaces of Spec (M). We also consider some topological aspects of certain frames. We prove that if R is a commutative ring and M is a multiplication R-module, then the lattice Semp (M/N) of semiprime submodules of M/N is a spatial frame for every submodule N of M. When M is a quasi projective module, we obtain that the interval ${\uparrow}(N)^{Semp}(M)=\{P{\in}Semp(M){\mid}N{\subseteq}P\}$ and the lattice Semp (M/N) are isomorphic as frames. Finally, we obtain results about quantales and the classical Krull dimension of M.
Castro, Ruth M.,Moreira, Lisela,Rojas, Maria R.,Gilbertson, Robert L.,Hernandez, Eduardo,Mora, Floribeth,Ramirez, Pilar The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.3
Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ~1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ~580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.
First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon
Castro, Marcela dos Santos,David, Maridelzira Betania Moraes,Goncalves, Evonnildo Costa,Siqueira, Andrei Santos,Virgulino, Rodrigo Rodrigues,Aguiar, Delia Cristina Figueira The Korean Society of Veterinary Science 2022 JOURNAL OF VETERINARY SCIENCE Vol.23 No.1
Background: Canine herpesvirus type 1 (CaHV-1) infects dogs and is associated with neonatal deaths and reproductive, ocular, neurological, and respiratory problems. In Brazil, reports of CaHV-1 have been restricted to the southeast and south regions, particularly in municipalities in the state of Rio Grande do Sul. Objectives: To assess the presence and variability of CaHV-1 in canine populations in the state of Pará, North Brazil. Methods: Biological samples from 159 dogs from 4 municipalities in the State of Pará were evaluated using polymerase chain reaction and phylogenetic analyses, with the target being the viral enzyme, thymidine kinase. Results: CaHV-1 was detected in 13 dogs (8.2%), with 2 animals being from the municipality of Santa Bárbara do Pará, 8 from Algodoal Island, 2 from Salinópolis, and one from Capanema. The study sequences revealed 100% identity among themselves and 64% to 100% identity with the other nucleotide sequences from Australia, Brazil, United Kingdom, and United States, including 100% identity with the 2002 isolate from Australia. The 1996 isolate from France was grouped in a branch that was different from the sequence of this study. Conclusions: This study presents the first molecular detection of CaHV-1 in dogs from the Amazon region in northern Brazil. The nucleotide identity between the strains and cytosine insertion in the sequences isolated in this study suggests at least 2 strains of CaHV-1 circulating in Brazil (Pará and BTU-1).
Castro, Maria,Losch, Pit,Park, Woojin,Haouas, Mohamed,Taulelle, Francis,Loerbroks, Claudia,Brabants, Gert,Breynaert, Eric,Kirschhock, Christine E. A.,Ryoo, Ryong,Schmidt, Wolfgang American Chemical Society 2018 Chemistry of materials Vol.30 No.8
<P>A case study on the understanding of the formation of hierarchical Beta zeolites using gemini-type piperidine based multiammonium surfactant (N<SUB>6</SUB>-diphe) is reported. Complementary techniques were used to investigate N<SUB>6</SUB>-diphe’s structure-directing effect at the molecular level. Combining characterization of the resulting zeolite materials with the toolboxes herein developed for studying clear solutions and dense gels discloses self-assembly processes that govern the growth (and growth inhibition) of nano-Beta zeolite crystals. In clear solution, small-angle X-ray scattering and liquid-state NMR provide insights about the formation of nanoparticles and their degree of order. <SUP>14</SUP>N and <SUP>1</SUP>H-DOSY NMR probe the dynamics and mobility of soluble species. In a dense gel, on the other side, <SUP>27</SUP>Al- and <SUP>29</SUP>Si-(MAS) NMR elucidate the varying local connectivity between initial nano-objects and the final solid products. It has been found that cylindrical micelles control the transformation of solubilized silica and alumina during the formation of zeolite nuclei and guide their crystal growth to nano-Beta rods with bimodal mesoporosity. The predominant smaller mesopores (6 to 8 nm) originate from the template’s hydrophobic alkyl chains, while larger mesopores (10 to 30 nm) are supposed to result from a spinodal decomposition-type segregation of phases consisting of as-formed hydrophobic zeolite rods and an aqueous solution.</P> [FIG OMISSION]</BR>
Castro, Ana Paula Gomes De,Goulart, Alexandre Moura Cintra,Andrade, Ednalva Patricia De,Cares, Juvenil Enrique,Carvalho, Daniel Diego Costa The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.3
Populations of plant-parasitic nematodes were evaluated in soil and root samples from areas planted with passion fruit, or covered with adjacent native cerrado vegetation, in five different cropping regions of the Brazilian Federal District. From July 2007 to January 2008, twenty samples had been collected in five Rural Centers (N$\acute{u}$cleo Rural-NR) planted with passion fruit. In five sites, samples were also collected in native cerrado vegetation. Specimens of nine genera of plant-parasitic nematodes (Meloidogyne, Helicotylenchus, Rotylenchulus, Scutellonema, Pratylenchus, Paratylenchus, Hemicycliophora, Xiphinema and Criconemoides) were found in the present survey. Important nematode pathogens, such as Rotylenchulus reniformis and Meloidogyne spp. were observed in the passion fruit plantations throughout five major producing regions of the Brazilian Federal District.