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de Boa Patrick Wesley Marques,Santos Kaiza de Sousa,de Oliveira Francisca Jennifer Duarte,Borges Boniek Castillo Dutra 대한치과보존학회 2024 Restorative Dentistry & Endodontics Vol.49 No.2
This study aimed to answer the question through a systematic review: Can carbamide peroxide be as effective as hydrogen peroxide and cause less in-office bleaching sensitivity? A literature survey was performed in PubMed/MEDLINE, Embase, Scopus, ISI Web of Science, and gray literature. Primary clinical trials that compared the efficacy or the in-office bleaching sensitivity between carbamide and hydrogen peroxides were included. The risk of bias was evaluated using the RoB2. The certainty of the evidence was assessed using the GRADE approach. DPI training significantly improved the mean scores of the dental undergraduates from 7.53 in the pre-DPI-training test to 9.01 in the post-DPI-training test (p < 0.001). After 6 weeks, the mean scores decreased marginally to 8.87 in the retention test (p = 0.563). DPI training increased their confidence level from 5.68 pre-DPI training to 7.09 post-DPI training. The limited evidence suggests that the 37% carbamide peroxide may be similarly effective to the 35% hydrogen peroxide for bleaching teeth in-office and causes less bleaching sensitivity. However, more well-designed split-mouth clinical trials are necessary to strengthen the evidence.
de Oliveira, Marcus Vinicius Linhares,Santos, Antonio Carvalho,Paulo, Graciano,Campos, Paulo Sergio Flores,Santos, Joana Korean Academy of Oral and Maxillofacial Radiology 2017 Imaging Science in Dentistry Vol.47 No.2
Purpose: The purpose of this study was to apply a newly developed free software program, at low cost and with minimal time, to evaluate the quality of dental and maxillofacial cone-beam computed tomography (CBCT) images. Materials and Methods: A polymethyl methacrylate (PMMA) phantom, CQP-IFBA, was scanned in 3 CBCT units with 7 protocols. A macro program was developed, using the free software ImageJ, to automatically evaluate the image quality parameters. The image quality evaluation was based on 8 parameters: uniformity, the signal-to-noise ratio (SNR), noise, the contrast-to-noise ratio (CNR), spatial resolution, the artifact index, geometric accuracy, and low-contrast resolution. Results: The image uniformity and noise depended on the protocol that was applied. Regarding the CNR, high-density structures were more sensitive to the effect of scanning parameters. There were no significant differences between SNR and CNR in centered and peripheral objects. The geometric accuracy assessment showed that all the distance measurements were lower than the real values. Low-contrast resolution was influenced by the scanning parameters, and the 1-mm rod present in the phantom was not depicted in any of the 3 CBCT units. Smaller voxel sizes presented higher spatial resolution. There were no significant differences among the protocols regarding artifact presence. Conclusion: This software package provided a fast, low-cost, and feasible method for the evaluation of image quality parameters in CBCT.
Panoramic radiographs underestimate extensions of the anterior loop and mandibular incisive canal
de Brito, Ana Caroline Ramos,Nejaim, Yuri,de Freitas, Deborah Queiroz,Santos, Christiano de Oliveira Korean Academy of Oral and Maxillofacial Radiology 2016 Imaging Science in Dentistry Vol.46 No.3
Purpose: The purpose of this study was to detect the anterior loop of the mental nerve and the mandibular incisive canal in panoramic radiographs (PAN) and cone-beam computed tomography (CBCT) images, as well as to determine the anterior/mesial extension of these structures in panoramic and cross-sectional reconstructions using PAN and CBCT images. Materials and Methods: Images (both PAN and CBCT) from 90 patients were evaluated by 2 independent observers. Detection of the anterior loop and the incisive canal were compared between PAN and CBCT. The anterior/mesial extension of these structures was compared between PAN and both cross-sectional and panoramic CBCT reconstructions. Results: In CBCT, the anterior loop and the incisive canal were observed in 7.7% and 24.4% of the hemimandibles, respectively. In PAN, the anterior loop and the incisive canal were detected in 15% and 5.5% of cases, respectively. PAN presented more difficulties in the visualization of structures. The anterior/mesial extensions ranged from 0.0 mm to 19.0 mm on CBCT. PAN underestimated the measurements by approximately 2.0 mm. Conclusion: CBCT appears to be a more reliable imaging modality than PAN for preoperative workups of the anterior mandible. Individual variations in the anterior/mesial extensions of the anterior loop of the mental nerve and the mandibular incisive canal mean that is not prudent to rely on a general safe zone for implant placement or bone surgery in the interforaminal region.
Lilian de Siqueira,Verônica Ribeiro dos Santos,Juliani Caroline Ribeiro de Araújo,Hugo Gutemberg Patiño de Oliveira Filho,Luana Marotta Reis de Vasconcellos,Eliandra de Sousa Trichês,Alexandre Luiz So 한국섬유공학회 2023 Fibers and polymers Vol.24 No.9
The development of new biomaterials with improved properties is a trend in tissue regeneration. In this way, an innovative approach is employed in this work for obtaining polymer fibers coated with nanoparticles resulting from the simultaneous application of poly (lactic acid) (PLA)/polycaprolactone (PCL) electrospinning and bioactive particles from an amorphous multicomponent silica–calcium–phosphorus system (AMS) electrospraying. The osteogenesis was evaluated in vitro and in vivo using male rats, in which total protein, alkaline phosphatase, and biological performance through histological and histomorphometric analysis were discussed. The morphological results assessed by scanning electron microscopy showed a mesh of PLA/PCL fibers associated with AMS. The spraying of 17.44% of AMS particles in the PLA/PCL electrospun fibers decreased the Young’s modulus and tensile strength. However, the amount of AMS particles sprayed was enough to promote a reduction of 17.8% in the measured contact angle values. Phosphatase alkaline higher mean value was also observed in the fibers when in contact with the AMS, but nonstatistical difference was observed (p > 0.05). It was possible to observe the presence of mineralized nodules deposited on the bottom of the plate and between the fibers. The newly formed bone into defect filled with PLA/PCL-AMS fibers was higher than that observed in the control group. These findings suggest PLA/PCL-AMS fibers as a multifunctional composite system that may be attractive for both bone and dental tissue engineering applications.
Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings
Jacqueline Oliveira dos Santos,Cinthia Aparecida Andrade,Kamila Rezende Dázio de Souza,Meline de Oliveira Santos,Isabel Rodrigues Brandão,Jose Donizeti Alves,Iasminy Silva Santos 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.3
Zinc is an essential micronutrient for the healthy development of plants, since its insufficient and supraoptimal doses can disrupt the metabolism and biomass production. We aimed to investigate the physiological responses of coffee seedlings to Zn deficiency and excess. Six-month-old seedlings were transferred to plastic pots containing a nutrient solution. The treatments were control (0.03 ppm), zinc deficiency (0.00 ppm), and zinc excess (0.12 ppm). The evaluations were performed in leaves and roots at the beginning of the treatments and after 30 and 60 d of treatments. Zn deficiency and excess increased the production of hydrogen peroxide, antioxidant enzymes activity, ascorbate, and lipid peroxidation contents. The imbalance in zinc nutrition reduced total chlorophyll content and increased carotenoids content throughout the experimental period. Lower biomass and proline accumulation were observed only for deficient seedlings at the end of the experiment. The characteristics analyzed showed that zinc deficiency caused greater damage to the Coffea arabica plants of (Catuai cultivar) than zinc excess.
Epizootic Infection by Trypanosoma vivax in Cattle from the State of Minas Gerais, Brazil
Matheus de Oliveira Reis,Fernanda Rezende Souza,Adriana Silva Albuquerque,Fernanda Monteiro,Luan Francisco dos Santos Oliveira,Djeison Lutier Raymundo,Flademir Wouters,Angélica Terezinha Barth Wouter 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2
Trypanosomiasis is caused by a pathogenic protozoan of the genus Trypanosoma, being Trypanosoma vivax the most important agent for cattle. The aim of the present study was to demonstrate the expansion of T. vivax infection in different mesoregions of Minas Gerais, Brazil, and describe the clinicopathological findings of trypanosomiasis in cattle. The diagnosis was based on visualization of the parasite in blood smears and DNA detection of T. vivax in the blood of live cows and tissues of necropsied animals by the polymerase chain reaction (PCR). Thirty suspected herds were tested, of which 11 were positive for T. vivax. The most frequent clinical signs were anemia, apathy, drop in milk production, weight loss, reproductive disorders, and nervous signs. Concomitant diseases, such as malignant edema, pneumonia and increased cases of mastitis were associated with T. vivax infection. Three cows were necropsied and the most significant findings were low body condition score, pale mucous and spleen with white pulp hyperplasia. The results demonstrated the expansion of T. vivax infection in Minas Gerais, that PCR-associated blood smears are promising for diagnosis, and that other diseases often occur concomitantly to T. vivax infection in regions with trypanosomiasis in cattle.
Marcelo de Oliveira, Santos,Albuquerque de Barros, Erika Valeria Saliba,Penha Tinoco, Maria Laine,Miranda Brasileiro, Ana Cristina,Lima Aragao, Francisco Jose The Korean Society of Plant Biotechnology 2002 Plant molecular biology and biotechnology research Vol.4 No.2
In order to achieve repetitive somatic embryogenesis in cacao (Theobroma cacao L.), callus derived from floral tissues were continuously cultured in a medium containing 2,4-D. In 5% of the explants, repetitive somatic embryogenesis was observed after 8 weeks and maintained in a globular stage for several weeks. This is the first report showing repetitive somatic embryogenesis in cacao. The calli were bombarded with a plasmid containing $\beta$-glucuronidase (gus) as reporter gene. Two week old calli showed the high average number of cells expressing the us gene. The effect of osmotic agents (mannitol, sorbitol and sucrose) on gene expression was evaluated. Pre-treatment during 16 h with 0.25 M mannitol revealed an improvement in gene expression. The potential utilization of the repetitive embryogenesis, combined with osmotic treatment, is discussed as an alternative to achieve stable transgenic cacao plants.
Acupuncture as Adjuvant Therapy for Sleep Disorders in Parkinson’s Disease
Fábio Henrique de Amorim Aroxa,Ihana Thaís Guerra de Oliveira Gondim,Elba Lúcia Wanderley Santos,Maria das Graças Wanderley de Sales Coriolano,Amdore Guescel C. Asano,Nadja Maria Jorge Asano 사단법인약침학회 2017 Journal of Acupuncture & Meridian Studies Vol.10 No.1
There are few studies which attest the efficacy of acupuncture on treatment of sleep disturbs in Parkinson disease. The aimed of this randomized clinical trial was to evaluate the effects of acupuncture on sleep disturbs of 22 patients with diagnosis of idiopathic Parkinson disease (Hoehn–Yahr 1 to 3) who have assistance on the Pro-Parkinson Program of Clinical Hospital at Federal University of Pernambuco in Brazil. All participants were evaluated by Parkinson Disease Sleep Scale (PDSS) before and after 8 weeks. The experimental group was submitted to 8 sections (once a week) which had duration of 30 minutes. The control group had no intervention. The intervention was executed using the acupuncture points LR3 (Taichong), SP6 (Sanyinjiao), LI4 (Hegu), TE5 (Wai-Guan), HT7 (Shenmen), PC6 (Neiguan), LI11 (Quchi), GB20 (Fengchi). Paired analyses were obtained by Wilcoxon test and independent analyses were made according to Mann–Whitney test. This study presented a potential therapeutic benefit of acupuncture on sleep disturbs of Parkinson's disease patients. This study showed a possible therapeutic benefit through acupuncture in sleep disorders in patients with PD. However, we propose new studies related to the effects of acupuncture on the clinical symptoms and evolution of the disease.
Antidiabetic Effect of the Chrysobalanus icaco L. Aqueous Extract in Rats
Ana Paula de Oliveira Barbosa,Gabriela de Oliveira Silveira,Igor Alexandre Cortes de Menezes,Jose´ Melquiades Rezende Neto,Jose´ Leal C. Bitencurt,Charles dos Santos Estavam,Amanda do Carmo Bion de Li 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.6
Chrysobalanus icaco L. is a medicinal plant popularly known in Brazil as ‘‘Grageru’’ or ‘‘Abageru.’’ It is used in African and American continents as medicinal food in the treatment of several diseases, including diabetes. This study used phytochemical screening to determine the antioxidant and α-amylase inhibitor activities of the aqueous extract (AECI) of C. icaco, and evaluated its antidiabetic potential in rodents. Phytochemical screening was performed using colorimetric tests with specific reagents. The in vitro antioxidant activity was evaluated by the scavenging activity of 2,2-diphenyl-1-picril-hydrazyl. The lethality test and behavioral screening was performed using an oral administration of 5 g/kg of AECI. The antidiabetic potential of AECI was evaluated through the oral glucose tolerance test (OGTT) and chronic hypoglycemic test at the doses of 100, 200, and 400 mg/kg (orally). Metformin was used as a reference drug in all tests. Diabetes was induced by injection of alloxan (40 mg/kg; intravenously). Phytochemical screening showed the presence of various compounds, including tannins, flavones, triterpenoids, steroids, saponins, and alkaloids. The in vitro antioxidant test demonstrated that AECI presented potent antioxidant activity. The lethality test and behavioral screening did not show lethality signs. In the OGTT test, AECI administration was not able to inhibit the elevation of glycemia. However, chronically administrated, it was able to cause a significant (P < .05) reduction of glycemia from 335 ± 27 up to 197 ± 15 mg/dL. These results demonstrate that the AECI presents a potential beneficial effect for diabetes.