The influence of nanofillers on the properties of ethanol-solvated and non-solvated dental adhesives

da Cruz, Leonardo Bairrada Tavares,Oliveira, Marcelo Tavares,Saraceni, Cintia Helena Coury,Lima, Adriano Fonseca The Korean Academy of Conservative Dentistry 2019 Restorative Dentistry & Endodontics Vol.44 No.3

Objectives: The aim of this study was to evaluate the influence of different concentrations of nanofillers on the chemical and physical properties of ethanol-solvated and non-solvated dental adhesives. Materials and Methods: Eight experimental adhesives were prepared with different nanofiller concentrations (0, 1, 2, and 4 wt%) and 2 solvent concentrations (0% and 10% ethanol). Several properties of the experimental adhesives were evaluated, such as water sorption and solubility (n = 5, 20 seconds light activation), real-time degree of conversion (DC; n = 3, 20 and 40 seconds light activation), and stability of cohesive strength at 6 months (CS; n = 20, 20 seconds light activation) using the microtensile test. A light-emitting diode (Bluephase 20i, Ivoclar Vivadent) with an average light emittance of $1,200mW/cm^2$ was used. Results: The presence of solvent reduced the DC after 20 seconds of curing, but increased the final DC, water sorption, and solubility of the adhesives. Storage in water reduced the strength of the adhesives. The addition of 1 wt% and 2 wt% nanofillers increased the polymerization rate of the adhesives. Conclusions: The presence of nanofillers and ethanol improved the final DC, although the DC of the solvated adhesives at 20 seconds was lower than that of the non-solvated adhesives. The presence of ethanol reduced the strength of the adhesives and increased their water sorption and solubility. However, nanofillers did not affect the water sorption and strength of the tested adhesives.

Control effects and sublethal effects of four pesticides on Atheloca bondari (Lepidoptera: Pyralidae) caterpillars

da Cruz Handel Kramel,de Almeida Paz-Neto Antônio,Monteiro Vaneska Barbosa,Gondim-Júnior Manoel Guedes Correa 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1

Atheloca bondari Heinrich is an opportunistic lepidopteran that uses necrosis caused by the mite Aceria guerreronis Keifer, the most important pest of the coconut palm, to access the meristematic region of coconuts. Because of this moth-mite positive association, pesticides used to control A. guerreronis (abamectin, azadirachtin, fenpyr oximate, and pyridaben) may impact A. bondari caterpillar behavior and/or survival. Thus, we used three methodologies (toothpick, triangle, and mesocarp fragment) to evaluate mortality caused by abamectin, the most commonly used pesticide to control A. guerreronis. The toothpick method proved to be suitable for toxicity tests of A. bondari caterpillars. Subsequently, the mortality caused by these four pesticides was evaluated, and abamectin caused 100% mortality in A. bondari caterpillars. Abamectin showed higher toxicity than the other pesticides to A. bondari caterpillars, with LC 50 and LC 95 values of 1.35 and 3.64 mg/L, respectively. The preference test showed that A. bondari caterpillars infested pesticide-treated fruits in the same proportion as untreated fruits. In the behavioral test, A. bondari caterpillars showed lower locomotor speed when exposed to surfaces with aba mectin, azadirachtin, and fenpyroximate residues. Our study presented that pesticides used to control A. guerreronis affect the behavior and survival of A. bondari caterpillars. We discuss how spraying these pesticides can indirectly control the population of A. bondari, reducing the impact of this moth on coconut production, and modifying the importance of this species as a pest of coconut palm.

Specificity of Multiplex PCR in the Detection of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola in Rice (Oryza sativa L.) Seeds

Da-Young Lee,Vera Cruz, C. M. 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.4

종자 등 유전자원의 국가간 교류는 새로운 병원균을 유입시켜 새로운 병을 일으키고 경제적인 손실을 극대화 시킬수 있는 원인을 제공하기 때문에 국가별로 검역이 더욱 강화되고 있는 실정이다. 그러한 연유로 국가별로는 자국에 존재하지 않은 유사한 병원균을 발견할 경우 종자도입을 막고 있어 불필요한 경제적 손실원인이 되기도 한다. 따라서 이러한 손실을 막기위해 각국에 검역소에서는 병원균의 형태적인 감별보다 더 정확한 검정기술이 시급한 실정이다. 본 연구에서는 다중PCR을 통하여 한번의 PCR 증폭으로 벼종자에 존재하는 벼흰잎마름병을 일으키는 Xanthomonas oryzae pv. oryzae (Xoo)과 세균성 줄무늬병을 일으키는 Xanthomonas oryzae pv.oryzicola (Xoc) 를 검출하고 이 두병원균을 판별할수 있는, 국제미작연구소가 최적화한 다중PCR 검정방법의 실질적 적용성 여부를 검증하기 위한 연구였다. 그 결과 51개의 비병원성이나 Xoo 또는 Xoc 와 유사하며 벼종자에서 분리된 균들은 다중PCR검정에서 어떤 밴드도 증폭하지 않은 반면, 대조구(positive control)에서는 알맞은 band size의 amplicon들을 증폭해냈다. 따라서 본 연구에 사용된 국제미작연구소에서 최적화된 다중 PCR방법 (IRRI-optimized multiplex PCR)은 벼종자에서 발견할수 있는 Xoo와 Xoc 의 존재 여부 뿐만 아니라 이들과 형태적으로 유사하나 비병원성균으로부터 구분해 내는데 탁월한 방법이었음을 확인할 수 있었다. One of the major hurdles faced by numerous quarantine centers is to accurately detect and distinguish non-plant pathogenic bacteria from plant pathogenic bacteria based on colony morphology. Yellow colony-forming, non-plant pathogenic bacteria found in rice seeds are often misidentified as Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), causal agents of bacterial leaf blight and bacterial leaf streak, respectively. In this study, 51 non-pathogenic, yellow-colony forming, Xoo and Xoc look-alike bacteria isolated from rice seeds, were used to test the specificity of a multiplex PCR for the detection and differentiation of Xoo and Xoc. Four primer pairs used in this multiplex PCR specific for Xanthomonas oryzae (Xo), Xoo and Xoc were used and all 51 isolates did not amplify any band, indicating that they are not Xo, Xoo or Xoc. These results imply that the multiplex PCR used in this study is robust in detecting and differentiating Xoo and Xoc from non-pathogenic, rice seed-associated, yellow colony-forming bacteria.

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

da Cruz-Filho, Antonio Miranda,de Vito, Angelo Rafael,Souza-Flamini, Luis Eduardo,da Costa Guedes, Debora Fernandes,Saquy, Paulo Cesar,Silva, Ricardo Gariba,Pecora, Jesus Djalma The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (${\alpha}=0.05$). Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Bivariate odd-log-logistic-Weibull regression model for oral health-related quality of life

Cruz, Jose N. da,Ortega, Edwin M.M.,Cordeiro, Gauss M.,Suzuki, Adriano K.,Mialhe, Fabio L. The Korean Statistical Society 2017 Communications for statistical applications and me Vol.24 No.3

We study a bivariate response regression model with arbitrary marginal distributions and joint distributions using Frank and Clayton's families of copulas. The proposed model is used for fitting dependent bivariate data with explanatory variables using the log-odd log-logistic Weibull distribution. We consider likelihood inferential procedures based on constrained parameters. For different parameter settings and sample sizes, various simulation studies are performed and compared to the performance of the bivariate odd-log-logistic-Weibull regression model. Sensitivity analysis methods (such as local and total influence) are investigated under three perturbation schemes. The methodology is illustrated in a study to assess changes on schoolchildren's oral health-related quality of life (OHRQoL) in a follow-up exam after three years and to evaluate the impact of caries incidence on the OHRQoL of adolescents.

Tucumã (Astrocaryum aculeatum) Prevents Oxidative and DNA Damage to Retinal Pigment Epithelium Cells

Beatriz da Silva Rosa Bonadiman,Cláudia Chaves,Charles Elias Assmann,Grazielle Castagna Cezimbra Weis,Audrei de Oliveira Alves,Amanda Leitão Gindri,Cláudio Chaves,Ivana Beatrice Mânica da Cruz,Ariane 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.10

Eye diseases have a negative impact on the eyesight quality of the world population. The age-related macular degeneration (AMD) draws special attention since it is a chronic disorder characterized by oxidative and inflammatory damage to the retinal epithelial pigment, which triggers progressive vision loss. In the Brazilian Amazon, Astrocaryum aculeatum is an Amazonian fruit (Tucumã) used by riverside communities in traditional medicine to treat a number of ailments. These communities have recently shown to have increased longevity and reduced prevalence of age-related morbidity. Thus, the aim of this research was to chemically characterize and analyze the in vitro antioxidant effect and molecular damage prevention of the Tucumã ethanolic extract in retinal pigment epithelium (RPE) cells in a model for AMD. The extract was chemically characterized by ultra-high-performance liquid chromatography (HPLC) coupled with diode-array detection and mass spectrophotometry (HPLC-DAD-MS). In vitro protocols were performed, and the cytopreventive effect of Tucumã on RPE cells exposed to high concentrations of superoxide anion, an oxidant and genotoxic molecule, as well as the effect of Tucumã extract on oxidative and molecular makers were assessed. Biochemical and flow cytometry analyses were conducted in these protocols. The extract presents high concentrations of caffeic acid, gallic acid, catechin, luteolin, quercetin, and rutin. Treatment did not show cytotoxic effects in cells treated only with extract at 50 μg/mL. In fact, it improved cell viability and was able to prevent necrosis and apoptosis, and oxidative and molecular damage was significantly reduced. In summary, Tucumã is an important Amazon fruit, which seems to contribute significantly to improve human health conditions, as our findings suggest that its extract has a relevant chemical matrix rich in antioxidant molecules, and its consumption could improve eye health and contribute to prevention against oxidative stress through cytoprevention, reactive oxygen species reduction, and maintenance of DNA integrity in retinal pigment epithelium (RPE) cells.

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

Antonio Miranda da Cruz-Filho,Angelo Rafael de Vito Bordin,Luis Eduardo Souza-Flamini,Débora Fernandes da Costa Guedes,Paulo César Saquy,Ricardo Gariba Silva,Jesus Djalma Pécora 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05). Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Low back pain, obesity, and inflammatory markers : exercise as potential treatment

Isabela Maia da Cruz Fernandes,Rafael Zambelli Pinto,Paulo Ferreira,Fábio Santos Lira 한국운동재활학회 2018 JER Vol.14 No.2

Portugal and China – Education Exchange and Cooperation

Rossana Alves da Cruz Duran 한국평화연구학회 2014 한국평화연구학회 학술회의 Vol.2014 No.1

Plant species from Brazilian Caatinga: a control alternative for Aedes aegypti

Carvalho Karine da Silva,Cruz Rômulo Carlos Dantas da,Souza Ivone Antônia de 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

The mosquito Aedes aegypti is the main vector for the virus dengue, chikungunya and Zika. For its control, it is essential to search for natural products with insecticidal effects. The climatic singularity of Caatinga, an exclusive Brazilian biome, aids the survival of plants that produce secondary metabolites, which could be toxic to insects. Therefore, this review discusses the insecticidal potential of Caatinga plants on Aedes aegypti mosquitoes. The meta-analysis was performed using Review Manager Software 5.4.1®. Several studies have demonstrated the insecticidal efficacy of Caatinga plants on the egg, larvae, pupae and adult phases of Ae. aegypti, with a pre dominance of the plant activity in the larval stage. The leaves were the most utilized part of the plant. The essential oils from Caatinga plants were significantly active against Ae. aegypti (RR = 0.21, 95 % CI = 0.07 – 0.68, p = 0.009). The most promising botanical genera as an insecticide are: Abarema, Myracrodruon, Croton, Lippia and Syagrus. Among chemical compounds from these insecticidal plants has been identified and isolated fla vonoids and fatty acids. Therefore, the Caatinga plant is a promising plant that contain bioactive compounds that are useful in the control of vector insects. This could contribute to the characterisation and valorisation of flora of this biome, as well as the production of environmentally friendly insecticides with specific action on target insects.