Clone and Identification of Bifunctional Δ12/Δ15 Fatty Acid Desaturase LKFAD15 from Lipomyces kononenkoae

Zhang Yan,Liang Zhuo,Jiang Mulan,Wan Xia,Gon Yangmin,Zhang Yinbo,Huang Fenghong 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2

In modern diet of human being, the ω-6/ω-3ratio of dietary fatty acid was shifted much towards ω-6series. In this work, a fatty acid desaturase (FAD) gene lkfad15 was cloned and identified from oleaginous yeast Lipomyces kononenkoae. Function analysis results shown that LKFAD15 is a novel Δ12/Δ15 bifunctional FAD which could not only produce linoleic acid and α-linolenic acid use just oleic acid as substrate but also adjust the ω-6/ω-3 fatty acids ratio to the WHO recommended ratio. Phylogenetic analysis of LKFAD15 suggested it is a specific intermediate product of gene evolution derives from independent gene duplication events.

Plasmonic and passivation effects of Au decorated RGO@CdSe nanofilm uplifted by CdSe@ZnO nanorods with photoelectrochemical enhancement

Zhang, Zhuo,Choi, Mingi,Baek, Minki,Deng, Zexiang,Yong, Kijung Elsevier 2016 Nano energy Vol.21 No.-

<P><B>Abstract</B></P> <P>Here, we demonstrate that the photoactivity of gold (Au)-decorated two-story hetero-nanostructures for photoelectrochemical (PEC) efficiency can be effectively enhanced by the plasmonic effect between Au and semiconductors in the visible region and by the passivation effect in the UV region. An Au-decorated two-story hetero-nanostructure was prepared as follows. The upper story is a novel hetero-nanofilm consisting of a reduced graphene oxide (RGO) nanofilm covered by a large area of crystalline CdSe nanolayer with a (111) plane outside and a thickness less than 8nm; the bottom story consists of CdSe-coated ZnO hetero-nanorods. In the visible region, the plasmonic effects between Au and other materials, such as ZnO, CdSe and RGO, were analyzed separately by reassembling the component ZnO nanorods, the CdSe layer and the RGO nanofilm. The photoconversion of the two-story hetero-nanostructure can be improved via the plasmonic effects between Au and n-type semiconductors, such as ZnO and CdSe, due to the stronger Schottky rectifier effects and hot-electron injection. In the UV light region, the photoconversion can be dramatically enhanced via the passivation of surface trap states. Moreover, the electron lifetime of the two-story hetero-nanostructure can also be improved by the suppression of electron–hole recombination by the Au nanoparticles and RGO nanofilms. Overall, CdSe-covered RGO hetero-nanofilms can increase the PEC efficiency 22.42 times over that of bare ZnO, while combination of the plasmonic and passivation effects can further improve this value by 20.7%, illustrating the potential of the two-story hetero-nanostructure in future photoelectrodes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The upper story is a hetero-nanofilm consisting of RGO covered by CdSe nanolayer less than 8 nm. </LI> <LI> The bottom story consists of CdSe-coated ZnO hetero-nanorods. </LI> <LI> In the visible region, photoconversion can be improved via plasmonic effects and Schottky contact. </LI> <LI> In the UV region, photoconversion can be enhanced via the passivation of surface trap states. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Diversity and geographic variation of endosymbiotic bacteria in natural populations of the pea aphid (Acyrthosiphon pisum) in China

Zhang Li,Cao Ya,Zhang Li-Jun,Wang Meng-Yao,Wang Xiao-Tong,Yang Xing-Zhuo,Duan Ting-Yu,Yuan Ming-Long 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Bacterial symbionts in aphids are known to benefit the insect host and associated with aphid’s ecological adaptation. The pea aphid (Acyrthosiphon pisum), an important legume pest worldwide, carries at least eight endosymbionts, providing a model system to study insect–bacteria interactions. However, species diversity and geographic variations of endosymbionts are unknown in Chinese populations; therefore, we characterized symbiont communities and diversity of 27 pea aphid samples from 13 geographic populations of China. Via amplicon high-throughput sequencing and diagnostic PCR, we found that bacterial communities of Chinese populations were dominated by Proteobacteria and Firmicutes. Among eight known endosymbionts, five (Buchnera, Serratia, Hamiltonella, Regiella, and Rickettsia) were detected by both methods, with a specific geographical distribution. The obligate symbiont, Buchnera, was present in all aphid samples, while the four facultative symbionts showed a significant geographic variation. Each population was randomly infected with distinct endosymbionts, ranging from three to five species. Serratia and Rickettsia showed relatively higher abundance in central regions of China, Regiella was predominant in eastern and western China, whereas Ham iltonella showed an extremely low abundance and was absent in four populations. Samples grouped by altitudes showed a significant diversity difference, whereas there was no significant difference between red and green body colors. Bacterial community structures of the Chinese pea aphid populations were mainly influenced by environmental factors, other than body colors. These data can guide the development of potential biocontrol techniques against this aphid.

Formation of Magnetic Graphene Nanosheets for Rapid Enrichment and Separation of Methyl Orange from Water

Zhang, Feng-Jun,Zhang, Zhuo,Xie, Fa-Zhi,Xuan, Han,Xia, Hong-Chen,Zhu, Lei,Oh, Won-Chun The Korean Ceramic Society 2014 한국세라믹학회지 Vol.51 No.6

Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heat treatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The results demonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attached nanoparticles were mainly $Fe_3O_4$. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10 mintues and would be practically usable for methyl orange separation from water.

A Rapidly New-typed Detection of Norovirus Based on F0F1-ATPase Molecular Motor Biosensor

Zhuo Zhao,Jie Zhang,Mei-Ling Xu,Zhi-Peng Liu,Hua Wang,Ming Liu,Yan-Yan Yu,Li Sun,Hui Zhang,Hai-Yan Wu 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1

In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycinbiotin- probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

Further-inspection for Doubly-Fed Converter: Theory, Method and Experimental Platform

Zhuo Chen,Zhengping Zhang,Zhenghang Hao,Shaohua Li,Ailing Zhang 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.6

Thermal replacement reaction: a novel route for synthesizing eco-friendly ZnO@γ-In₂Se₃hetero-nanostructures by replacing cadmium with indium and their photoelectrochemical and photocatalytic performances

Zhang, Zhuo,Choi, Mingi,Baek, Minki,Yong, Kijung The Royal Society of Chemistry 2015 Nanoscale Vol.7 No.19

<P>A novel route called thermal replacement reaction was demonstrated for synthesizing eco-friendly ZnO@γ-In2Se3 hetero-structural nanowires on FTO glass by replacing the element cadmium with indium for the first time. The indium layer was coated on the surface of the ZnO nanowires beforehand, then CdSe quantum dots were deposited onto the coated indium layer, and finally the CdSe quantum dots were converted to γ-In2Se3 quantum dots by annealing under vacuum at 350 °C for one hour. The prepared ZnO@γ-In2Se3 hetero-nanostructures exhibit stable photoelectrochemical properties that can be ascribed to the protection of the In2O3 layer between the ZnO nanowire and γ-In2Se3 quantum dots and better photocatalytic performance in the wide wavelength region from 400 nm to nearly 750 nm. This strategy for preparing the ZnO@γ-In2Se3 hetero-nanostructures not only enriches our understanding of the single replacement reaction where the active element cadmium can be replaced with indium, but also opens a new way for the in situ conversion of cadmium-based to eco-friendly indium-based nano-devices.</P>

Effect of Xeroderma Pigmentosum Complementation Group F Polymorphisms on Gastric Cancer Risk and Associations with H.pylori Infection

Zhang, Ji-Shun,Zhang, Chuan,Yan, Xue-Yan,Yuan, Zhi-Fang,Duan, Zhuo-Yang,Gao, Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

We conducted a hospital case-control study by genotyping four potential functional single nucleotide polymorphisms (SNPs) to assess the association of Xeroderma pigmentosum complementation group F (XPF) with gastric cancer susceptibility, and role of XPF polymorphisms in combination with H.pylori infection in risk definition. A total of 331 patients with gastric cancer and 355 controls were collected. Four SNPs of XPF, rs180067, rs1799801, rs2276466 and rs744154, were genotyped by Taqman real-time PCR method with a 7900 HT sequence detector system. The gastric cancer patients were more likely to have smoking habit, a family history of cancer and H.pylori infection. We did not find any significant difference in the genotype distributions of XPF rs180067, rs1799801, rs2276466 and rs744154 between cases and controls. However, multivariate logistic analysis showed a non-significant decreased risk in patients carrying rs180067 G allele, rs1799801 T allele or rs2276466 T allele genotypes. A non-significant increased risk of gastric cancer was found in individuals carrying the rs744154 GG genotype. Stratification by H.pylori infection and smoking was not significantly different in polymorphisms of XPF rs180067, rs1799801, rs2276466 and rs744154. The four XPF SNPs did not show significant interaction with H.pylori infection and smoking status (P for interaction was 0.35 and 0.18, respectively). Our study indicated that polymorphisms in rs180067, rs1799801, rs2276466 and rs744154 may affect the risk of gastric cancer but further large sample size studies are needed to validate any association.

An unconventional outer-to-inner synthesis strategy for core (Au)-shell nanostructures with photo-electrochemical enhancement

Zhang, Zhuo,Baek, Minki,Song, Hongseon,Yong, Kijung The Royal Society of Chemistry 2017 Nanoscale Vol.9 No.16

<P>In this work, an outer-to-inner strategy is demonstrated to simultaneously fabricate core-shell NPs and assemble them onto a scaffold. Specifically, the shell material is deposited onto the scaffold first, and then a layer of the core material (Au) is covered on the shell surface. Finally, the core (Au)-shell nanoparticles (NPs) are formed on the scaffold after annealing. As examples, Au-Bi2S3, Au-CdS and Au-CdSe coreshell NPs are grown on the surface of ZnO nanorods (NRs) via this strategy and exhibit enhanced photoelectrochemical (PEC) efficiency. The enhanced PEC performance is ascribed to improved light absorption induced by the plasmonic effect, trapped electrons of Au NPs, and cascade band alignment of the shell material and ZnO. The synthetic method gives a universal route to the development of nanodevices with assembled core-shell NPs. The core-shell NPs in the current study possess significant potential as building blocks for future PEC anodes or other solar conversion systems.</P>

Clinical Efficacy and Prognosis Factors for Advanced Hepatoblastoma in Children: A 6-year Retrospective Study

Zhang, Yi,Zhang, Wei-Ling,Huang, Dong-Sheng,Hong, Liang,Wang, Yi-Zhuo,Zhu, Xia,Hu, Hui-Min,Zhang, Pin-Wei,Yi, You,Han, Tao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: This study aimed to investigate the effect of multimodality treatment of advanced paediatric hepatoblastoma (HB) and the factors affecting prognosis. Methods: A total of 35 children underwent multimodality treatments consisting of chemotherapy, surgery, interventional therapy, and autologous peripheral blood stem cell transplantation. The patients were followed up every month. Results: Serum AFP levels in 33 out of 35 patients in this study were significantly increased (P = 0.0002). According to the statistical scatter plot, the values of serum AFP on the 25th, 50th, and 75th percentages were 1,210, 1,210 and 28,318 ng/dl, respectively. Of the 35 cases, 21 were stage IV. 18 cases were treated with systemic chemotherapy before surgery, and 3 cases with locally interventional chemotherapy before surgery. Statistical analysis showed that the preferred interventional treatment affected prognosis, and that there was a statistically significant difference (P = 0.024). Some 33 patients completed the follow-up, of which 17 were in complete remission (CR), 5 were in partial remission (PR), 1 became disease progressive (DP), and 10 died. The remission and overall survival rates were 66.7% (22/33) and 69.7% (23/33), respectively. Patients with the mixed HB phenotypes had worse prognoses than the epithelial phenotype (P < 0.001), and patients in stage IV had a lower survival rate than those in stage III (P < 0.001). Conclusion: Multimodality treatment can effectively improve remission rate and prolong the survival of children with advanced HB. In addition, alpha-fetoprotein (AFP), a tumor marker of liver malignant tumors, HB pathological classification, and staging are highly useful in predicting prognosis.