http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Isolation and Characterization of Collagen from the Muscle of Amur Sturgeon (Acipenser schrenckii)
Zhenbin Wang,Lin Wang,Shiman Lin,Qiufang Liang,Zhujun Shi,Junmin Xu,Haile Ma 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
The collagen in Amur sturgeon muscle wasisolated using sodium chloride (salt-solubilized collagen,SSC, 3.02%), followed by acetic acid (acid-solubilizedcollagen, ASC, 31.56%) and then pepsin (pepsin-solubilizedcollagen, PSC, 58.49%). The collagens appeared to bedense sheet-like film linked by random-coiled filamentsunder SEM. There was no obvious difference in denaturationtemperature (about 33°C) assessed by CD, while themelting temperature of SSC (115.82°C) was significantlylower than ASC and PSC (120.23 and 118.80°C, respectively)determined by DSC. SDS-PAGE showed that the collagenswere mainly type I with similar amino acid profiles. FTIRconfirmed the triple helical structure of the collagens,and indicated more hydrogen bond in PSC and moreintermolecular crosslinks in ASC. These results providesome basis for elucidating the function of collagen in thedevelopment of meat texture during postmortem aging andprocessing.
Yuanyuan Chen,Zhujun Wang,Yuping Cheng,Yongmin Tang 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.4
Hemophagocytic lymphohistiocytosis (HLH) in different ethnicities has been described in the literature, but few cases in patients of Chinese descent have been reported. Here, we describe the case of a Chinese neonate presenting with HLH carrying novel, compound heterozygous mutations of the UNC13D gene, including [c.2295_2298delGCAG, p.Glu765Aspfs*27] in exon 23, c.-250C>T, c.1+30G>A, c.279C>T, c.888G>C, c.18+36A>G, c.20-48T>C, c.1977C>T, c.2296C>T, c.24-46C>T, c.26-9_26-8insC, c.2599A>G, c.28+48C>T and c.3198A>G, some of which have not been reported in the literature. Cytokine profile analyses were performed in this patient, and the results were consistent with our previous findings in HLH patients. Cytokine profile monitoring may be helpful in differentiating among various clinical phases of HLH.
Yunxiang Zang,Hao Zhang,Linghui Huang,Fang Wang,Fei Gao,Xishan Lv,Jing Yang,Biao Zhu,홍승범,Zhujun Zhu 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.6
Glucosinolates have attracted considerable attention in the past several decades because of their involvement in pathogen and insect resistance of Brassicaceae as well as anti-inflammatory and anti-oxidation activity in humans. Methyl jasmonate (MeJA) treatment generally increases the levels of indole and aromatic glucosinolates; however, the time course of MeJA effects on the levels of individual glucosinolates in different pak choi cultivars has not been studied. Here, we found that foliar MeJA application increased accumulation of indole glucosinolates in both leaves and roots of four cultivars. However, roots accumulated much higher levels of indole glucosinolates and, compared to leaves, showed a delayed response to MeJA in terms of indole glucosinolate accumulation in all four cultivars. Individual indole glucosinolate levels in roots differed depending on the sampling time in different cultivars. The level of neoglucobrassicin steadily increased over 72 h. Glucobrassicin was induced in leaves of SHQ and HZYDE, and in roots of three cultivars, whereas 4-methoxyglucobrassicin was induced only in roots. Aromatic glucosinolate enrichment occurred only in roots. Aliphatic glucosinolates were detected only in the leaves but not in roots, accumulating to comparable levels as in untreated controls. These results suggest that exogenous MeJA-mediated signal transduction did not immediately lead to biosynthesis of aliphatic and aromatic glucosinolates but did induce increased accumulation of indole glucosinolates in pak choi. Individual glucosinolate accumulation profiles were different depending oncultivars.
Tian Guozhong,Zhang Li,Li Machao,Wang Xiaolei,Zheng Yuhong,Li Xiaojing,Huang Cheng,Li Xuechun,Xie Yongqiong,Xu Li,Ren Hongyu,Shao Zhujun 한국미생물학회 2009 The journal of microbiology Vol.47 No.4
Two hundred and seventy-three Haemophilus influenzae strains isolated from pediatric pneumonia patients in China were studied. We used Multilocus Sequence Typing (MLST) to analyze genotypic characteristics. All strains were biotyped and serotyped. Relatedness and patterns of genes among isolates were determined by the analysis of MLST and eBURST. H. influenzae primarily causes acute pneumonia in children under 1 year old. Nontypeable H. influenzae was responsible for most cases of pediatric pneumonia. All 273 strains were classified into eight biotypes. They mostly belonged to the I, II, and III biotypes (17.6%, 43.6%, and 22.7%, respectively). 62 strains (22.7%) produced β-lactamase. We found 28 novel alleles. Fifty different STs were found by MLST, of which 39 were novel. These were ST477 through ST508 and ST521 through ST527. Group 17 and predicted founders 503 were new groups in this study. No STs correlated with strains from Korea, which is adjacent to China. The H. influenzae strains from China appeared to have heterogeneous ST types patterns which may be the reason no outbreaks or epidemics of H. influenzae infections have occurred in Chengdu city, Sichuan, China.
Lejun Zhang,Bo Zhang,Ran Guo,Zhujun Wang,Guopeng Wang,Jing Qiu,Shen Su,Yuan Liu,Guangxia Xu,Zhihong Tian,Sergey Gataullin 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.4
With the development of covert communication technologies, the number of covert communication technologies using blockchain as a carrier is increasing. However, using the transaction amount of digital currency as a carrier for covert communication has problems such as low embedding rate, large consumption of transaction amount, and easy detection. In this paper, firstly, by experimentally analyzing the distribution of bitcoin transaction amounts, we determine the most suitable range of amounts for matrix decomposition. Secondly, we design a novel matrix decomposition method that can successfully decompose a large amount matrix into two small amount matrices and utilize the elements in the small amount matrices for covert communication. Finally, we analyze the feasibility of the novel matrix decomposition method in this scheme in detail from four aspects, and verify it by experimental comparison, which proves that our scheme not only improves the embedding rate and reduces the consumption of transaction amount, but also has a certain degree of resistance to detection.
Graphene Oxide Nanosheet-Composited Poly(N-isopropylacrylamide) Hydrogel for Cell Sheet Recovery
Yongqing Xia,Han Wu,Dachao Tang,Shuai Gao,Binghe Chen,Zhujun Zeng,Shengjie Wang,Meiwen Cao,Dongxiang Li 한국고분자학회 2019 Macromolecular Research Vol.27 No.7
Cell sheet engineering technique has been applied to treat various tissues without the use of a traditional scaffold. To date, methods for the cell sheet harvesting depend mostly on grafted poly(N-isopropylacrylamide) (pNIPAAm) thin layers, while the native pNIPAAm hydrogel, which possibly presents the easiest way to prepare thermo-responsive materials, is not suitable for the cell sheet harvesting due to its low cell attachment. In this study, the graphene oxide (GO) nanosheet was utilized as an additive to enhance the bio-compatibility of the pNIPAAm hydrogel. Different concentrations of GO nanosheets were added to prepare GO/pNIPAAm composite hydrogels through the in-situ free radical polymerization with polyethylene glycol dimethacrylate (PEGDA) as a cross-linker. The results indicated that the physical properties of the composite hydrogels had little difference with that of the native pNIPAAm hydrogel. However, the cell attachment, proliferation and detachment behaviors on the composite hydrogel surface were greatly enhanced. Monkey fibroblast COS7 cells attached and proliferated better on the GO/pNIPAAm composite hydrogel, while intact COS7 cell sheets could be harvested from the composite hydrogels by simply lowering the temperature. In contrast, the cells appeared as clusters on the native pNIPAAm hydrogel. Furthermore, when HeLa and COS7 cells were seeded successively onto the micropatterned GO/pNIPAAm hydrogel, there could be the formation of a patterned HeLa/COS7 cell layer. The geometrically patterned GO/pNIPAAm hydrogel may provide an easy-to-prepare material for releasing patterned cell sheets compared to the specific cell-adhesive proteins reported to make patterned cell layers.