Preparation and Drug Release Behavior of Tussah Silk Fibroin Composite Membrane

Zhuang Hu,Kaixia An,Jia Wang,Xiaoxu Xu,Zhangpei Chen,Jianshe Hu,Liqun Yang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2

Silk fibroin (SF) aqueous solution was obtained by the degumming and dissolution of tussah silk, and then the obtained solution was chemically cross-linked with sodium alginate (SA) to form composite membrane, which could be used as a carrier for sustained drug release. The mechanical properties and dissolution rate of such composite membrane togetherwith the drug release behavior under different conditions were investigated. A series of structure-tunable composite membranes were prepared through altering the ingredients proportion, the pH value during fabrication, the concentration of Ca2+, and cross-linking time. The drug release behavior of the corresponding drug-loaded composite membrane was elaborate studied using artemisinin as the model drug. It was found that the drug release rate exhibited a decreasing trend with increasing the SF content, the Ca2+ concentration and the cross-linking time of the corresponding composite membrane. Moreover, the drug release behavior exhibited pH sensitivity, and the drug release rate could be promoted under weak alkaline conditions.

Energy-saving Methods in the Operation of Virtual Networks Based on Divided Time Slices

HU Ying,ZHUANG Lei,HU Yu-xiang,MA Ding 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.7

Reducing energy consumption has also become an important way to reduce expenditures and increase revenue for ISP. For solving the problem of dynamically changing network-flow of virtual network in runtime, we first analyze relations and differences between virtual network and IP network on energy-saving issues. We secondly model the makeup of energy consumption in virtual network, and determine the object of energy-saving in virtual network at. Finally we propose the solutions to this energy-saving problem. The solutions can be described as follows: (1) based on the historical data of daily network-flow, we divide one day into two time slices, and one is called idle time slice, the other is called busy time slice; (2) we design the dynamic switch-off algorithm and run it at the beginning of idle time slice, and we use former algorithms to be started at the beginning of busy time slice; (3) in the dynamic switch-off algorithm we determine the method to select virtual networks and the method to remap; (4) we apply roulette wheel to select virtual networks, in the selection method we consider both current influences on energy-saving and influences in the future. Simulation results show that the dynamic switch-off algorithm can effectively select virtual networks, and the whole solution performs well in energy aware virtual network in runtime.

A Reflective Heterogeneous Service Coordination Middleware Based On Mobile Agents in Wireless Environments

Haiyang Hu,Hua Hu,Yi Zhuang,Lianghuai Yang 보안공학연구지원센터 2008 International Journal of Software Engineering and Vol.2 No.4

Software services distributed in open wireless environments are implemented using different middleware types and advertised using different discovery protocols. Client application needs to adopt a flexible approach to discover and bind these services. Compared with other related work, this paper presents a new reflective coordination middleware based on mobile agent. It dynamically adapts both its binding and discovery protocol to allow interoperation with heterogeneous services by uploading different functional components. By dynamically creating subagents at run time, it can interoperate with multiple services in parallel. As a result, the client burden is released, and the efficiency of the coordination process is enhanced.

Proteomic Profiles of Mouse Neuro N2a Cells Infected with Variant Virulence of Rabies Viruses

( Wang Xiao Hu ),( Shou Feng Zhang ),( Cheng Long Sun ),( Zi Guo Yuan ),( Xian Fu Wu ),( Dong Xia Wang ),( Zhuang Ding ),( Rong Liang Hu ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.4

We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using twodimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 θ/δ, and downregulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ≥2 times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS- 11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus`` adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.

Fixed-accuracy confidence interval estimation of P(X > c) for a two-parameter gamma population

Zhuang, Yan,Hu, Jun,Zou, Yixuan The Korean Statistical Society 2020 Communications for statistical applications and me Vol.27 No.6

The gamma distribution is a flexible right-skewed distribution widely used in many areas, and it is of great interest to estimate the probability of a random variable exceeding a specified value in survival and reliability analysis. Therefore, the study develops a fixed-accuracy confidence interval for P(X > c) when X follows a gamma distribution, Γ(α, β), and c is a preassigned positive constant through: 1) a purely sequential procedure with known shape parameter α and unknown rate parameter β; and 2) a nonparametric purely sequential procedure with both shape and rate parameters unknown. Both procedures enjoy appealing asymptotic first-order efficiency and asymptotic consistency properties. Extensive simulations validate the theoretical findings. Three real-life data examples from health studies and steel manufacturing study are discussed to illustrate the practical applicability of both procedures.

An Effective WSSENet-Based Similarity Retrieval Method of Large Lung CT Image Databases

Yi Zhuang,Shuai Chen,Nan Jiang,Hua Hu 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.7

With the exponential growth of medical image big data represented by high-resolution CT images(CTI), the high-resolution CTI data is of great importance for clinical research and diagnosis. The paper takes lung CTI as an example to study. Retrieving answer CTIs similar to the input one from the large-scale lung CTI database can effectively assist physicians to diagnose. Compared with the conventional content-based image retrieval(CBIR) methods, the CBIR for lung CTIs demands higher retrieval accuracy in both the contour shape and the internal details of the organ. In traditional supervised deep learning networks, the learning of the network relies on the labeling of CTIs which is a very time-consuming task. To address this issue, the paper proposes a Weakly Supervised Similarity Evaluation Network (WSSENet) for efficiently support similarity analysis of lung CTIs. We conducted extensive experiments to verify the effectiveness of the WSSENet based on which the CBIR is performed.

An Alkaline pH Control Strategy for Methionine Adenosyltransferase Production in Pichia pastoris Fermentation

Xiaoqing Hu,Ju Chu,Si-Liang Zhang,Ying-ping Zhuang,Xin Wu,Huaxin Chen,Zhongyuan Lv,Zhongyi Yuan 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5

Pichia pastoris is a successful system forexpressing heterologous proteins and its fermentation pH isalways maintained below 7.0. However, particular proteinsare unstable under acidic conditions, such as methionineadenosyltransferase (MAT), and thus fermentation underacidic pH conditions is unsuitable because protein activityis lost owing to denaturation. Here, a strategy employingalkaline pH in the late fermentation period was developedto improve MAT production. Initially, P. pastoris KM71was transformed with the mat gene to overexpress MAT. After 72 h of in vitro incubation at different pH values, theexpressed MAT displayed highest stability at pH 8.0;however, pH 8.0 inhibited cell growth and induced cellrupture, thus affecting protein production. To balance MATstability and Pichia cell viability, different pH controlstrategies were compared. In strategy A (reference), theinduction pH was maintained at 6.0, whereas in strategy B,it was gradually elevated to 8.0 through a 25 h transitionperiod (80 ~ 105 h). MAT activity was 0.86 U/mg (twofoldhigher than the control). However, MAT content wasreduced by 50% when compared with strategy A, becauseof proteases released upon cell lysis. To improve cellviability under alkaline conditions, glycerol was added inaddition to methanol (strategy C). When compared withstrategy B, the MAT-specific activity remained nearlyconstant, whereas the expression level increased to 1.27 g/L. The alkaline pH control strategy presented herein for MATproduction represents an excellent alternative for expressingproteins that are stable only under alkaline conditions.

Preparation of Ionic Liquid-modified SBA-15 Doped with Molybdovanadophosphoric Acid for Oxidative Desulfurization

Jiangzhou Zhuang,Xiaoyin Jin,Xianlin Shen,Junjun Tan,Longhui Nie,Jian Xiong,Bing Hu 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.7

A series of catalysts, ionic liquid-modified SBA-15 (denoted ILSBA) doped with H5PMo10V2O40 (HPMoV2) have been synthesized and characterized by XRD, FT-IR, 1H NMR, TG-DTA, and TEM. The catalyst was used for the removal of dibenzothiophene (DBT) in model oil combined with hydrogen peroxide (the oxidant) and acetonitrile (the phase-transfer agent). It was observed that the sulfur content of DBT can be reduced from 500 to 2 ppm by adjusting the amount of catalyst, the reaction temperatures and the reaction time. Besides, the catalyst activity for different sulfur compounds showed a huge difference which may be mainly affected by the electron densities of sulfur atom. Moreover, the catalyst can be recycled seven times without a significant loss in activity, which could be ascribed to the strong electrostatic interaction between ILSBA and HPMoV2. In addition, a postulated mechanism was proposed to reveal the oxidative desulfurization process.

Static and fatigue behavior of through-bolt shear connectors with prefabricated HFRC slabs

Yuliang He,Jie Zhuang,Lipu Hu,Fuyou Li,Ying Yang,Yi-qiang Xiang 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.1

Twelve push-out test specimens were conducted with various parameters to study the static and fatigue performance of a new through-bolt shear connector transferring the shear forces of interface between prefabricated hybrid fiber reinforced concrete (HFRC) slabs and steel girders. It was found that the fibers could improve the fatigue life, capacity and initial stiffness of through-bolt shear connector. While the bolt-hole clearance reduced, the initial stiffness, capacity and slippage of through-bolt shear connector increased. After the steel-concrete interface properties were improved, the initial stiffness increased, and the capacity and slippage reduced. Base on the test results, the equation of the load-slip curve and capacity of through-bolt shear connector with prefabricated HFRC slab were obtained by the regression of test results, and the allowable range of shear force under fatigue load was recommended, which could provide the reference in the design of through-bolt shear connector with prefabricated HFRC slabs.

A comparative study of the dendritic avalanche in MgB2 thin films synthesized by pulsed laser deposition and hybrid physical chemical vapor deposition methods

Lee, Jae-Yeap,Lee, Hu-Jong,Lee, Sung-Ik,Zhuang, C. G.,Wang, Y. Z.,Feng, Q. R.,Gan, Z. Z.,Xi, X. X.,Choi, Eun-Mi,Cho, Jae-Hun,Jo, Young-Hun American Institute of Physics 2009 JOURNAL OF APPLIED PHYSICS - Vol.105 No.8