Identification of QTLs associated with the anaerobic germination potential using a set of Oryza nivara introgression lines

Licheng Liu,Xiaoxiang Li,Sanxiong Liu,Jun Min,Wenqiang Liu,Xiaowu Pan,Baohua Fang,Min Hu,Zhongqi Liu,Yongchao Li,Haiqing Zhang 한국유전학회 2021 Genes & Genomics Vol.43 No.4

Background Rice (Oryza sativa L.) is an important crop and a staple food for half of the population around the world. The recent water and labor shortages are encouraging farmers to shift from traditional transplanting to direct-seeding. However, poor germination and slow elongation of the coleoptile constrains large-scale application of direct-seeding. Objective Thisstudy was aimed to investigate the genetic basis of the anaerobic germination(AG) potential using a set of Oryza nivara (O. nivara) introgressionlines (ILs). Methods Inthis study, a total of 131 ILs were developed by introducing O. nivara chromosomesegments into the elite indica rice variety 93-11 through advanced backcrossingand repeated selfng. A high-density genetic map has been previouslyconstructed with 1,070 bin-markers. The seeds of ILs were germinated and usedto measure coleoptile length under normal and anaerobic conditions. QTLsassociated with AG potential were determined in rice. Results Basedon the high-density genetic map of the IL population, two QTLs, qAGP1 and qAGP3 associated with AG tolerance were characterized and locatedon chromosomes 1 and 3, respectively. Each QTL explained 15% of the phenotypic variance.Specifcally, the O. nivara-derived chromosomesegments of the two QTLs were positively tolerance to anaerobic condition byincreasing coleoptile length. In a further analysis of public transcriptomedata, a total of 26 and 36 genes within qAGP1 and qAGP3 were transcriptionallyinduced by anaerobic stress, respectively. Conclusions Utilizationof O. nivara-derived alleles at qAGP1 and qAGP3 can potentially enhance tolerance to anaerobic stress at thegermination stage in rice, thereby accelerating breeding of rice varieties tobe more adaptative for direct-seeding.

Extraction separation of toluene/cyclohexane with hollow fiber supported ionic liquid membrane

Fan Zhang,Zhongqi Ren,Wei Sun,Junteng Liu,Weidong Zhang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.6

A supported liquid membrane with ionic liquid was used for the separation of toluene/cyclohexane. Theinteractions of ionic liquid with toluene and cyclohexane were calculated and experimentally studied by quantum chemicalcalculation and liquid-liquid extraction process. The results showed [BPy][BF4] have stronger interaction with toluenethan that with cyclohexane. The selectivity of SILM processes was larger than 10 at the temperature of 323 K and theflow rate of 13.5 mL·min-1 on both shell side and lumen side. Due to the higher viscosity of IL, SILM process hadgood long-term stability. As the effects of mass transfer driving force of SILM process, the flux and removal efficiencyincreased with increase of initial toluene concentration, while the selectivity decreased because of the competitive transport. Base on the resistance in-series model and experimental results, the mass transfer resistance was mainly lay liquidmembrane phase. The influence of flow rates on both sides was slight. The higher temperature could enhance the masstransfer performance significantly. The removal efficiency increased from 28.2% to 45.1% with the increasing of operationtemperature from 298 K to 323 K.

LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.

Atractylodin induces apoptosis through downregulation of PI3Kγ-mediated PI3K/Akt/mTOR/p70S6K signalling in colon cancer cells and suppresses the tumour formation in xenograft mice model

Wenyi Lu,Jianxia Liu,Bin Wu,Shungen Huang,Jian Wang,Runda Wu,Zhongqi Mao 한국고분자학회 2024 Macromolecular Research Vol.32 No.2

This study used both in vitro and in vivo models to evaluate the efficacy of atractylodin as an anticancer treatment for colorectal cancer. The cytotoxicity of atractylodin on colon cancer cells was assessed using the MTT assay, and atractylodininduced apoptosis was determined using flow cytometry. The expression of cleaved caspase 3 and other apoptotic proteins was examined using Western blotting to determine the mechanism underlying atractylodin's anticancer activity. In addition, the role of PI3K/Akt/mTOR/p70S6K signalling in atractylodin-induced apoptosis in colon cancer cells was analyzed. The study found that atractylodin caused dose-dependent ROS-mediated apoptosis and DNA damage in colon cancer cells and activated caspase 3. Furthermore, atractylodin inhibited the PI3K/Akt/mTOR/p70S6K signalling pathway by targeting PI3Kγ in colon cancer cells. Molecular docking analysis indicated that atractylodin binds to the Akt binding pocket of PI3Kγ. The study also evaluated the antitumour effects of atractylodin on a colon cancer tumour xenograft model and found that it significantly reduced tumour growth and volume by inducing apoptosis. These results suggest that atractylodin has potential as a candidate for the treatment of colorectal cancer, although further research is necessary.

Immobilization of Copper in Brown Soil Using Different Amendments

Shiwei Zhou,Zhihong Yu,Fei Lian,Zhongqi Liu,Hua Zhang,Zhengguo Song 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Filtration and regeneration behavior of polytetrafluoroethylene membrane for dusty gas treatment

Weidong Zhang,Deqiang Jiang,Junteng Liu,Zhongqi Ren,Wang Geng 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.4

With micron talcum particles and nano-CaCO3 powder as test dust, a series of experiments have been carried out to systematically study the gas filtration and regeneration behavior of polytetrafluoroethylene membrane, and some comparisons were made with common filter media. The experimental results showed that the PTFE membrane had a filtration efficiency of above 99.99% for micron particles, and excellent regeneration behavior was obtained, though a much higher initial pressure drop existed. Based on the results, it was concluded that the PTFE membrane is an excellent surface-filtration media for micron particles. Effects of operation parameters, including airflow velocity, particle concentration and particle characteristics were also investigated. To better understand the evolution of pressure drop during the filtration process, a mathematical model with operation parameters and characteristics of particles was derived from the gas-solid two-phase flow theories. A novel method on the determination of regeneration period of the filter media was put forward based on the analysis of the pressure drop according to this model