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( Yan Zhang ),( Jingjing Wang ),( Chen Yajun ),( Minghui Zhou ),( Wei Wang ),( Ming Geng ),( Decong Xu ),( Zhongdong Xu ) 한국균학회 2020 Mycobiology Vol.48 No.2
The carbohydrate-active enzyme (CAZyme) genes of Trametes contribute to polysaccharide degradation. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters (BGCs) of Trametes remain unclear. Here, we conducted comparative analysis, detected the CAZyme genes, and predicted the BGCs for nine Trametes strains. Among the 82,053 homologous clusters obtained for Trametes, we identified 8518 core genes, 60,441 accessory genes, and 13,094 specific genes. A large proportion of CAZyme genes were cataloged into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes were divided into six strategies, and the nine Trametes strains harbored 47.78 BGCs on average. Our study revealed that Trametes exhibits an open pan-genome structure. These findings provide insights into the genetic diversity and explored the synthetic biology of secondary metabolite production for Trametes.
Bubble formation in a step-emulsification microdevice: hydrodynamic effects in the cavity
Zhiwei Zhang,Zhongdong Wang,Fengrui Bao,Mengyu Fan,Shaokun Jiang,Chunying Zhu,Youguang Ma,Taotao Fu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-
This study focuses on the generation of bubbles in a step-emulsification microdevice via a two-anglephotography method. It's found that the bubble generation mechanism is controlled by the interfacialtension, below a critical capillary number; while controlled by viscous force, inertial force and thedisturbance induced by the bubble swarm, above the critical capillary number. From the two-anglephotography method, a model is established for predicting the bubble size, by taking into account of thedynamic contact angle between gas-liquid interface and wall, and the hydrodynamic feedback of thecavity on bubble formation via the quantification of resistance by the volume fraction of gas in the cavity.
Ontology-Based Cutting Tool Configuration Considering Carbon Emissions
Guanghui Zhou,Qi Lu,Zhongdong Xiao,Ce Zhou,Shengze Yuan,Chao Zhang 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.11
In order to improve the precision and efficiency of cutting tool configuration and reduce carbon emissions during manufacturing process, an ontology-based cutting tool configuration process considering carbon emissions is put forward in the paper. Firstly, the architecture of ontology-based cutting tool configuration is established and key functional modules are described. Secondly, ontology is applied to describe the complex knowledge of cutting tool configuration and the Semantic Web Rule Language (SWRL) is used to build inference rules to reason feasible cutting tool configuration schemes according to machining requirements. Thirdly, taking carbon emissions as the objective, an evaluation method based on the c-PBOM-T (carbon emissions-Process Bill of Material for cutting Tools) table is studied to decide an optimal cutting tool configuration scheme from the feasible ones in the previous step for part machining. Finally, the proposed method is applied to a vortex shell workpiece to demonstrate its feasibility. The results show that the proposed method can improve the cutting tool configuration and reduce carbon emissions effectively for the machining processes. The presented method provides a valuable insight into the intelligent cutting tool configuration to support low-carbon manufacturing.
High performance phosphorus-modified ZSM-5 zeolite for butene catalytic cracking
Xionghou Gao,Zhicheng Tang,Haitao Zhang,Conghua Liu,Zhongdong Zhang,Gongxuan Lu,Dong Ji 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.3
Phosphorus-modified ZSM-5 zeolites were prepared with a novel method, hydrothermal dispersion. XRD showed that the catalysts prepared by the hydrothermal dispersion had better hydrothermal stability than that by impregnation. At the same time, more pronounced cracking activity and higher yield of ethylene plus propylene were obtained on the phosphorus-modified ZSM-5 catalysts prepared by hydrothermal dispersion. Highest yield of ethylene plus propylene was obtained when the loading of phosphorus was 0.68%. The higher amount of phosphorus fixed on the pores of zeolite, higher hydrothermal stability and appropriate acid amount were the possible reasons for obtaining higher yield of ethylene plus propylene.
Zhong, Dong,Ran, Jian-Hua,Tang, Wen-Yuan,Zhang, Xiao-Dong,Tan, Yun,Chen, Gui-Jie,Li, Xiao-Song,Yan, Yi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
Invasion is usually recognized as the main reason for the high recurrence and death rates of glioma and restricts the efficacy of surgery and other therapies. Therefore, we aimed to investigate the mechanism involved in promotion effects of mda-9/syntenin on human glioma cell migration. The wound healing method was used to test the migration ability of human glioma cells CHG-5 and CHG-hS, stably overexpressing mda-9/syntenin. Western blotting was performed to determine the expression and phosphorylation of focal adhesion kinase (FAK) and JNK in CHG-5 and CHG-hS cells. The migration ability of CHG-hS cells was significantly higher than that of CHG-5 cells in fibronectin (FN)-coated culture plates. Phosphorylation of FAK on tyrosine 397, 576, and 925 sites was increased with time elapsed in CHG-hS cells. However, phosphorylated FAK on the tyrosine 861 site was not changed. Phosphorylated Src, JNK and Akt levels in CHG-hS cells were also significantly upregulated. Phosphorylation of JNK and Akt were abolished by the specific inhibitors SP600125 and LY294002, respectively, and the migration ability of CHG-hS cells was decreased, indicating that the JNK and PI3K/Akt pathways play important roles in regulating mda-9/syntenin-induced human brain glioma migration. Our results indicate Mda-9/syntenin overexpression could activate FAK-JNK and FAK-Akt signaling and then enhance the migration capacity of human brain glioma cells.