A putative pathogen-resistant regulatory pathway between MicroRNAs and candidate target genes in maize

Jian Gao,Mao Luo,Chun Zhang,Hua Peng,Haijian Lin,Yaou shen,Maojun Zhao,Guangtang Pan,Zhiming Zhang 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4

MicroRNAs (miRNAs) are a family of small non-coding RNAs that, in most cases, negatively regulate gene expression at the post-transcriptional level. Plant miRNAs have been implicated in developmental processes and adaptation to environmental stress including biotic and abiotic stresses. Here, we report a comprehensive analysis of miRNAs and associated target genes under banded leaf and sheath blight (BLSB) stress caused by R. solani in maize. Eight differentially expressed miRNAs were randomly selected from deep sequencing results and validated by qRT-PCR together with their putative target genes, most of which are transcription factors as well as metabolic genes involved in auxin signaling. The results revealed that majorities of the analyzed miRNAs show an inverse correlation with their corresponding predicted target genes. In addition, a putative regulatory network of miRNAs-mRNAs responsive to R. solani was constructed. This study provides insight into the regulatory functions of miRNAs, thereby expanding our knowledge of the molecular mechanisms of pathogen resistance.

A comparison of the fracture resistances of endodontically treated mandibular premolars restored with endocrowns and glass fiber postcore retained conventional crowns

Jing Guo,Zhiming Wang,Xuesheng Li,Chaoyang Sun,Erdong Gao,Hongbo Li 대한치과보철학회 2016 The Journal of Advanced Prosthodontics Vol.8 No.6

PURPOSE This in-vitro study aimed to evaluate the fracture resistances and failure modes of endodontically treated mandibular premolars restored with endocrowns and conventional post-core retained crowns. MATERIALS AND METHODS Thirty mandibular premolars were assigned into three groups (n=10): GI, intact teeth; GE, teeth with endocrowns; GC, teeth with conventional post-core supported crowns. Except for the teeth in group GI, all specimens were cut to 1.5 mm above the cementoenamel junction and endodontically treated. Both endocrowns and conventional crowns were fabricated from lithium-disilicate blocks using a CEREC 3D CAD/CAM unit. All specimens were subjected to thermocycling and then to 45° oblique compressive load until fracture occurred. The fracture resistance and failure mode of each specimen were recorded. Data were analyzed with one-way ANOVA and LSD Post Hoc Test (α=.05). RESULTS The fracture resistances of GE and GC were significantly lower than that of GI (P<.01), while no significant difference was found between GE and GC (P=.702). As of the failure mode, most of the specimens in GE and GC were unfavorable while a higher occurrence of favorable failure mode was presented in GI. CONCLUSION For the restoration of mandibular premolar, endocrown shows no advantage in fracture resistance when compared with the conventional method. Both of the two methods cannot rehabilitate endodontically treated teeth with the same fracture resistances that intact mandibular premolars have.

The Single Center Experience of One Thousand Cases of Robotic Pancreatectomy

( Rong Liu ),( Sai Chou ),( Zhiming Zhao ),( Guodong Zhao ),( Yuanxing Gao ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Operation plan for pancreatic tumor used to be divided in four main type,pancreaticoduodenectomy (PD),central pancreatectomy,distal pancreatectomy (DP) and pancreatic enucleation. To evaluate the value of da Vinci robotic surgery system in pancreatectomy. Methods: The clinical data of a consecutive of 1000patients who underwent robotic-assisted pancreatectomy from Novem-ber, 2011 to September 2017 were retrospectively reviewed. All the operations were performed by the same team, which mainly approach in Da Vinci robotic system Si. The demographic characteristics, intraoperative blood loss, operative duration, post-operative hospital stay, blood transfusion details, and conversion rate were all analyzed. Results: A total of 1000 patients were included in this study, with an average age of 54.0, and 486 cases of them were male. The average operative time was 3.5 hours (2-7 h), the estimated blood loss was 100ml (20-1200 ml), and the postoperative hospital stay was 9.3 days in average. Overall complication morbidity was 7.9% (79/1000) and 6.7%(67/1000) cases suffered from grade B-C Pancreatic fistula, with the conversion rate of 3.2% and 30-Day mortality rate of 0.42%. Conclusions: Robotic pancreatectomy could be successfully performed with the assistance of da Vinci surgery system, the operative duration, post-operative hospital stay and intraoperative blood loss were satisfying, with very patients died in 30 days and very operations converted.

Malus toringoides (Rehd.) Hughes Ameliorates Nonalcoholic Fatty Liver Disease with Diabetes via Downregulation of SREBP-1c and the NF-κB Pathway In Vivo and In Vitro

Mi Xie,Liying Gao,Zhiming Liu,RuiYing Yuan,Dongzhi Zhuoma,Dikye Tsering,Yuefei Wang,Shan Huang,Bin Li 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.12

Diabetic patients are more prone to developing nonalcoholic fatty liver disease (NAFLD) compared with healthy people. As a plant homologous to both medicine and food, Malus toringoides (Rehd.) Hughes has been used as an intervention for both NAFLD and diabetes. However, the effect and mechanism of M. toringoides on NAFLD on type 2 diabetes mellitus (T2DM) is unclear. The current investigation was designed to evaluate the ameliorative effects and mechanism of M. toringoides ethanol extract (CBTM-E375) on T2DM, and to identify the compounds in these extracts. The effects of CBTM-E375 on T2DM were verified using a high-fat diet-/streptozotocin-induced diabetic rat and free fatty acid (0.5 mM)-induced human hepatocellular carcinoma cell (HepG2) models. The components of CBTM-E375 were identified by high performance liquid chromatography-mass spectrometry/mass spectrometry. Our results demonstrate that CBTM-E375 ameliorated lipid accumulation (total cholesterol, triglyceride), oxidative stress (superoxide dismutase, catalase, malondialdehyde, glutathione peroxidase), and inflammation (tumor necrosis factor-α [TNF-α], interleukin [IL]-1β, IL-6, C-reactive protein [CRP]) in vivo and in vitro, these effects were associated with a CBTM-E375-mediated downregulation of SREBP-1c (sterol regulatory element binding protein 1c) and the NF-κB (nuclear factor κB) signaling pathway. A total of 20 chemical compounds were identified in CBTM-E375, including phlorizin, isoquercitrin, chlorogenic acid, quercetin, naringenin, and trigonelline, which have been reported to have positive effects on diabetes or on NAFLD.

A comparison of the fracture resistances of endodontically treated mandibular premolars restored with endocrowns and glass fiber post-core retained conventional crowns

Guo, Jing,Wang, Zhiming,Li, Xuesheng,Sun, Chaoyang,Gao, Erdong,Li, Hongbo The Korean Academy of Prosthodonitics 2016 The Journal of Advanced Prosthodontics Vol.8 No.6

PURPOSE. This in-vitro study aimed to evaluate the fracture resistances and failure modes of endodontically treated mandibular premolars restored with endocrowns and conventional post-core retained crowns. MATERIALS AND METHODS. Thirty mandibular premolars were assigned into three groups (n=10): GI, intact teeth; GE, teeth with endocrowns; GC, teeth with conventional post-core supported crowns. Except for the teeth in group GI, all specimens were cut to 1.5 mm above the cementoenamel junction and endodontically treated. Both endocrowns and conventional crowns were fabricated from lithium-disilicate blocks using a CEREC 3D CAD/CAM unit. All specimens were subjected to thermocycling and then to $45^{\circ}$ oblique compressive load until fracture occurred. The fracture resistance and failure mode of each specimen were recorded. Data were analyzed with one-way ANOVA and LSD Post Hoc Test (${\alpha}=.05$). RESULTS. The fracture resistances of GE and GC were significantly lower than that of GI (P<.01), while no significant difference was found between GE and GC (P=.702). As of the failure mode, most of the specimens in GE and GC were unfavorable while a higher occurrence of favorable failure mode was presented in GI. CONCLUSION. For the restoration of mandibular premolar, endocrown shows no advantage in fracture resistance when compared with the conventional method. Both of the two methods cannot rehabilitate endodontically treated teeth with the same fracture resistances that intact mandibular premolars have.

A Three-Dimensional ZnO/CdS/NiFe Layered Double Hydroxide Photoanode Coupled with a Cu2O Photocathode in a Tandem Cell for Overall Solar Water Splitting

Jia Liu,Yinghua Zhang,Zhiming Bai,Zhian Huang,Yukun Gao,Yuan Yao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11

An integrated tandem photoelectrochemical (PEC) cell, composed of a three-dimensional (3D) ZnO/CdS/NiFe layered double hydroxide (LDH) core/shell/hierarchical nanowire arrays (NWAs) photoanode and a p-Cu2O photocathode, was designed for unassisted overall solar water splitting in this study. The optical and photoelectrochemical characteristics of ZnO-based photoanodes and Cu2O photocathode were investigated. The results show that ZnO/CdS/NiFe LDH nanostructures offer significantly enhanced performances with a photocurrent density reaching 5.8 mA · cm -2 at 0.9 V and an onset potential as early as 0.1 V (versus RHE). The enhancement can be attributed to the existence of CdS nanoparticles (NPs) which boosts the light absorption in visible region and enhances charge separation. Moreover, the introduction of NiFe LDH nanoplates, with unique hierarchical mesoporous architecture, promotes electrochemical reactions by providing more active sites as co-catalyst. On the above basis, the ZnO/CdS/NiFe LDH–Cu2O two-electrode tandem cell system was established. At zero bias, the device shows a photocurrent density of 0.4 mA · cm -2 along with the corresponding solar-to-hydrogen (STH) conversion efficiency reaching 0.50%. Our results indicate that the tandem PEC cells consisting of metal–oxide–semiconductor photoelectrodes based on Earth-abundant and low-cost materials hold promising application potential for overall solar water splitting.

In situ evaluation of corrosion damage using non-destructive electrochemical measurements—A case study

Sina S. Jamali,Yue Zhao,Zhiming Gao,Huijun Li,Ay Ching Hee 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-

Field application of electrochemical measurements with a non-invasive electrode arrangement has beenused for investigating corrosion damage and protectiveness of a paint coating on an artwork sculpture. For thisfield application solid state probes were designed and used in electrochemical impedancespectroscopy (EIS) and electrochemical noise measurement (ENM) to evaluate the level of corrosionprotection afforded by the paint coating. Samples were also analyzed in laboratory using analyticaltechniques. Results of electrochemical measurements indicated poor protective properties of the paintcoating while analytical examinations revealed an inhomogeneous structure of the coating and a highlyporous structure of the oxide scale.

The Single Center Experience of Two Hundred and Thirty One Cases of robotic pancreaticoduodenectomy

Liu Rong,Tang Wenbo,Zhao Zhiming,Zhao Guodong,Tan Xianglong,Gao Yuanxing 한국간담췌외과학회 2017 한국간담췌외과학회 학술대회지 Vol.2017 No.3

Synthesis of Vinyl Sulfides and Vinylamines through Catalytic Intramolecular Hydroarylation in the Presence of FeCl₃ and AgOTf

Eom, Dahan,Mo, Juntae,Lee, Phil Ho,Gao, Zhiming,Kim, Sunggak WILEY‐VCH Verlag 2013 European Journal of Organic Chemistry Vol.2013 No.3

<P><B>Abstract</B></P><P>A synthetic method was developed for the preparation of vinyl sulfides and vinylamines from arylalkynyl phenyl sulfides and sulfonamides. Under mild conditions, a catalytic intramolecular hydroarylation reaction was carried out in the presence of FeCl<SUB>3</SUB> and AgOTf (OTf = trifluoromethanesulfonate) in 1,2‐dichloroethane. A variety of 1,2‐dihydronaphthalenes, 2<I>H</I>‐chromenes, and 1,2‐dihydroquinolines containing a phenylsulfenyl or <I>N</I>‐phenyl‐<I>N</I>‐tosyl group on the sp<SUP>2</SUP>‐hybridized benzylic carbon were prepared in good to excellent yields. The present method could be extended to the preparation of dihydropyrano[2,3‐<I>g</I>]chromenes through a twofold Fe‐catalyzed hydroarylation by a selective 6‐<I>endo</I> mode.</P>

Photoelectrocatalytic Oxidation of Methane into Methanol Over ZnO Nanowire Arrays Decorated with Plasmonic Au Nanoparticles

Jia Liu,Yinghua Zhang,Zhian Huang,Zhiming Bai,Yukun Gao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2

The pursuit for efficient conversion of methane under ambient conditions remains a challenge. Here, we reported photoelectrocatalytic oxidation of methane into methanol over ZnO nanowire arrays (NWAs) decorated with Au nanoparticles (NPs) under simulated sunlight illumination with ambient conditions. The photoelectrochemical (PEC) performances of the ZnO and ZnO/Au photoanodes were investigated to analyze the behavior and intensity of the reaction process of methane oxidation. The Faradaic efficiency of ZnO/Au was calculated to be 32.11%, nearly three times of 11.69% for ZnO. The above results show that ZnO NWAs exhibited exceptional activity as photoanode for photoelelctrocatalytic methane oxidation, and the decoration of Au NPs further enhanced the photo-activity via the surface plasmon resonance expanding its absorption spectra to visible region. On the other hand, as a co-catalyst, Au can promote the oxidation of methane by providing the trapping sites and active sites to facilitate the separation and also suppress the recombination of photogenerated charges and the existence of Au can boost the reaction by lowering the activation energy. This research demonstrates that ZnO NWAs decorated with Au NPs hold great promise for photoelectrocatalytic methane oxidation.