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Yue Ma,Qiyao Wang,Xiating Gao,Yuanxing Zhang 한국미생물학회 2017 The journal of microbiology Vol.55 No.1
Fish pathogen Vibrio anguillarum, a mesophile bacterium, is usually found in estuarine and marine coastal ecosystems worldwide that pose a constant stress to local organism by its fluctuation in salinity as well as notable temperature change. Though V. anguillarum is able to proliferate while maintain its pathogenicity under low temperature (5–18°C), so far, coldadaption molecular mechanism of the bacteria is unknown. In this study, V. anguillarum was found possessing a putative glycine betaine synthesis system, which is encoded by betABI and synthesizes glycine betaine from its precursor choline. Furthermore, significant up-regulation of the bet gene at the transcriptional level was noted in log phase in response to cold-stress. Moreover, the accumulation of betaine glycine was only found appearing at low growth temperatures, suggesting that response regulation of both synthesis system and transporter system are cold-dependent. Furthermore, in-frame deletion mutation in the two putative ABC transporters and three putative BCCT family transporters associated with glycine betaine uptake could not block cellular accumulation of betaine glycine in V. anguillarum under coldstress, suggesting the redundant feature in V. anguillarum betaine transporter system. These findings confirmed that glycine betaine serves as an effective cold stress protectant and highlighted an underappreciated facet of the acclimatization of V. anguillarum to cold environments.
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The Single Center Experience of One Thousand Cases of Robotic Pancreatectomy
( Rong Liu ),( Sai Chou ),( Zhiming Zhao ),( Guodong Zhao ),( Yuanxing Gao ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Operation plan for pancreatic tumor used to be divided in four main type,pancreaticoduodenectomy (PD),central pancreatectomy,distal pancreatectomy (DP) and pancreatic enucleation. To evaluate the value of da Vinci robotic surgery system in pancreatectomy. Methods: The clinical data of a consecutive of 1000patients who underwent robotic-assisted pancreatectomy from Novem -ber, 2011 to September 2017 were retrospectively reviewed. All the operations were performed by the same team, which mainly approach in Da Vinci robotic system Si. The demograph ic characteristics, intraoperative blood loss, operative duration, post-operative hospital stay, blood transfusion details, and con version rate were all analyzed. Results: A total of 1000 patients were included in this study, with an average age of 54.0, and 486 cases of them were male. The average operative time was 3.5 hours (2-7 h), the estimated blood loss was 100ml (20-1200 ml), and the postop erative hospital stay was 9.3 days in average. Overall complica tion morbidity was 7.9% (79/1000) and 6.7%(67/1000) cases suffered from grade B-C Pancreatic fistula, with the conversion rate of 3.2% and 30-Day mortality rate of 0.42%. Conclusions: Robotic pancreatectomy could be successfully performed with the assistance of da Vinci surgery system, the operative duration, post-operative hospital stay and intraoper ative blood loss were satisfying, with very patients died in 30 days and very operations converted.
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