Effects of DMSO/LiCl Dissolution on the Structural Characteristics of Bamboo Lignin

Wenjuan Wu,Jia Sun,Zhiguo Wang,Yongcan Jin 한국펄프·종이공학회 2016 한국펄프·종이공학회 학술발표논문집 Vol.2016 No.10

Severe choline deficiency induces alternative splicing aberrance in optimized duck primary hepatocyte cultures

Zhao Lulu,Cai Hongying,Wu Yongbao,Tian Changfu,Wen Zhiguo,Yang Peilong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline. Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity <i>in vitro</i> and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model.Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes.Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of <i>alb</i> and <i>afp</i> and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism.Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.

A Linear Iteration Image Restoration Method Based on Homology Continuity

Yonghua Zhu,Shunyi Mao,Pin Wu,Honghao Gao,Zhiguo Wu 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

A novel image restoration method based on homology continuity is proposed in this paper. We view images as a collection of gray scale points, taking advantage of the homology continuity principle to combine each point and its fuzzy point derived by drop mass function to constitute the path direction and obtain distinct restore points. The gather of all the restore points is the sharply focused image of the original image. At last, we found every picture by iteration method is clearer than the last by experiment with the method proposed in the paper. The result verifies its feasibility.

Kinetic Evaluation of Products Inhibition to Succinic Acid Producers Escherichia coli NZN111, AFP111, BL21, and Actinobacillus succinogenes 130ZT

Qiang Li,Dan Wang,Yong Wu,Maohua Yang,Wangliang Li,Jianmin Xing,Zhiguo Su 한국미생물학회 2010 The journal of microbiology Vol.48 No.3

Succinic acid is one of the platform compounds and its production via natural feedstocks has drawn worldwide concerns. To evaluate the inhibitory effects of fermentation products on the growth of Actinobacillus succinogenes 130ZT and Escherichia coli NZN111, AFP111, BL21, fermentations with addition of individual products in medium were carried out. The cell growth was inhibited when the concentrations of formate, acetate, lactate, and succinate were at range of 8.8-17.6 g/L, 10-40 g/L, 9-18 g/L, and 10-80 g/L, respectively. For these two species of bacteria, E. coli was more resistant to acid products than A. succinogenes, while both endured succinate rather than by-products. As a result of end product inhibition, succinate production yield by A. succinogenes decreased from 1.11 to 0.49 g/g glucose. Logistic and Monod mathematical models were presented to simulate the inhibition kinetics. The Logistic model was found more suitable for describing the overall synergistic inhibitory effects.

Design, synthesis, and evaluation of bitopic arylpiperazine-phthalimides as selective dopamine D₃ receptor agonists

Cao, Yongkai,Sun, Ningning,Zhang, Jiumei,Liu, Zhiguo,Tang, Yi-zhe,Wu, Zhengzhi,Kim, Kyeong-Man,Cheon, Seung Hoon The Royal Society of Chemistry 2018 MedChemComm Vol.9 No.9

<P>The dopamine D3 receptor (D3R) is a proven therapeutic target for the treatment of neurological and neuropsychiatric disorders. In particular, D3R-selective ligands that can eliminate side effects associated with dopamine D2 receptor (D2R) therapeutics have been validated. However, the high homology in signaling pathways and the sequence similarity between D2R and D3R have rendered the development of D3R-selective ligands challenging. Herein, we designed and synthesized a series of piperazine-phthalimide bitopic ligands based on a fragment-based and molecular docking inspired design. Compound 9i was identified as the most selective D3R ligand among these bitopic ligands. Its selectivity was improved compared to reference compounds 1 and 2 by 9- and 2-fold, respectively, and it was 21-fold more potent than compound 2. Molecular docking demonstrated that the orientation of Leu<SUP>2.64</SUP> and Phe<SUP>7.39</SUP> and the packing at the junction of helices may affect the specificity for D3R over D2R. Functional evaluation revealed that D3R-selective ligand 9i displayed a subpicomolar agonist activity at D3R with a 199-fold increase in potency compared to quinpirole. These results may be useful for the fragment-based design of bitopic compounds as selective D3R ligands.</P>

A Hybrid Link Protection Scheme for Ensuring Network Service Availability in Link-state Routing Networks

Haijun Geng,Han Zhang,Xingang Shi,Zhiliang Wang,Xia Yin,Ju Zhang,Zhiguo Hu,Yong Wu 한국통신학회 2020 Journal of communications and networks Vol.22 No.1

The internet is playing an increasingly crucial role inboth personal and business activities. In addition, with the emergenceof real-time, delay sensitive and mission-critical applications,stringent network availability requirement is put forward for internetservice providers (ISPs). However, commonly deployed intradomainlink-state routing protocols react to link failures by globallyexchanging link state advertisements and recalculating routingtable, inevitably causing significant forwarding discontinuityafter a failure. Therefore, the loop-free criterion (LFC) approachhas been widely deployed by many ISPs for coping with the singlenetwork component failure scenario in large internet backbones. The success of LFC lies in its inherent simplicity, but this comesat the expense of letting certain failure scenarios go unprotected. To achieve full failure coverage with LFC without incurring significantextra overhead, we propose a novel link protection scheme,hybrid link protection (HLP), to achieve failure resilient routing. Compared to previous schemes, HLP ensures high network availabilityin a more efficient way. HLP is implemented in two stages. Stage one provides an efficient LFC based method (MNP-e). Thecomplexity of the algorithm is less than that of Dijkstra’s algorithmand can provide the similar network availability with LFC. Stage two provides backup path protection (BPP) based on MNP-e,where only a minimum number of links need to be protected, usingspecial paths and packet headers, to meet the network availabilityrequirement. We evaluate these algorithms in a wide spread ofrelevant topologies, both real and synthetic, and the results revealthat HLP can achieve high network availability without introducingconspicuous overhead. HLP not only needs around 10% time ofthat of full protection, but also provides full protection capabilitiesthat full protection provide.

Increased Cognition Connectivity Network in Major Depression Disorder: A fMRI Study

Ting Shen,Cao Li,Biao Wang,Wei-min Yang,Chen Zhang,Zhiguo Wu,Mei-hui Qiu,Jun Liu,Yi-feng Xu,Dai-hui Peng 대한신경정신의학회 2015 PSYCHIATRY INVESTIGATION Vol.12 No.2

ObjectiveaaEvidence of the brain network involved in cognitive dysfunction has been inconsistent for major depressive disorder (MDD), especially during early stage of MDD. This study seeks to examine abnormal cognition connectivity network (CCN) in MDD within the whole brain. MethodsaaSixteen patients with MDD and 16 health controls were scanned during resting-state using 3.0 T functional magnetic resonance imaging (fMRI). All patients were first episode without any history of antidepressant treatment. Both the left and right dorsolateral prefrontal cortex (DLPFC) were used as individual seeds to identify CCN by the seed-target correlation analysis. Two sample t test was used to calculate between-group differences in CCN using fisher z-transformed correlation maps. ResultsaaThe CCN was constructed by bilateral seed DLPFC in two groups separately. Depressed subjects exhibited significantly increased functional connectivity (FC) by left DLPFC in one cluster, overlapping middle frontal gyrus, BA7, BA43, precuneus, BA6, BA40, superior temporal gyrus, BA22, inferior parietal lobule, precentral gyrus, BA4 and cingulate gyrus in left cerebrum. Health controls did not show any cluster with significantly greater FC compared to depressed subjects in left DLPFC network. There was no significant difference of FC in right DLPFC network between depressed subjects and the health controls. ConclusionaaThere are differences in CCN during early stage of MDD, as identified by increased FCs among part of frontal gyrus, parietal cortex, cingulate cortex, and BA43, BA22, BA4 with left DLPFC. These brain areas might be involved in the underlying mechanisms of cognitive dysfunction in MDD.