Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat

Zhi-Fu Guo,Feng-Zhen Li,Xiao-Gang Ma,Feng Lin,Hui Ma,Li-Jing Chen,Ming Zhong,Li-Ping Bai,Ying Yi 한국유전학회 2011 Genes & Genomics Vol.33 No.5

Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis,Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile,TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.

Optimal Design of Intelligent Material for the Active Vibration Control

Zhi Guo Ma,Yun Hui Yan,Yao Fu,Bang Chun Wen 대한기계학회 1997 Asia-Pacific Vibration Conference Vol.2 No.1

Overexpression of NDRG2 Can Inhibit Neuroblastoma Cell Proliferation through Negative Regulation by CYR61

Zhang, Zhi-Guo,Li, Gang,Feng, Da-Yun,Zhang, Jian,Zhang, Jing,Qin, Huai-Zhou,Ma, Lian-Ting,Gao, Guo-Dong,Wu, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Several recent studies have showed that the n-myc downstream regulated gene 2 (NDRG2) is a new tumor suppressor gene, and that it plays an important role in tumor suppression in several cancers or cancer cell lines. However, few studies focused on its function in neuroblastoma cells. In the present investigation, we demonstrated that NDRG2 overexpression inhibited their proliferation. Using a cDNA microarray, we found that overexpression of NDRG2 inhibited the expression of cysteine-rich protein 61 (CYR61), a proliferation related gene. From our research, CYR61 may partially hinder NDRG2-mediated inhibition of cell proliferation. Overexpression of NDRG2 resulted in accumulation of cells in the G1 phase, which was accompanied by upregulation of p21 and p27 and downregulation of CDK4 and cyclin D1. Taken together, these data indicate that NDRG2 inhibits the proliferation of neuroblastoma cells partially through suppression of CYR61. Our findings offer novel insights into the physiological roles of NDRG2 in neuroblastoma cell proliferation, and NDRG2 may prove to be effective candidate for the treatment of children with neuroblastoma.

Effects of dietary Enteromorpha powder on reproduction-related hormones and genes during the late laying period of Zi geese

Ma Wei Qing,Zhao Dan Hua,Cheng Huang Zuo,Wang Si Bo,Yang Ji,Hong Xia Cui,Lu Ming Yuan,Wu Hong Zhi,Xu Li,Liu Guo Jun 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.3

Objective: The aim of this study was to investigate the effects of Enteromorpha powder supplementation on reproduction-related hormones and genes in the late laying period of Zi geese. Methods: A total of 312 (1-year-old) Zi geese with similar laying rate were randomly divided into 2 groups with 6 replicates each, each with 21 female geese and 5 male geese. The control group was fed with a basal diet and the test group was fed with a diet containing 3% Enteromorpha powder. The trial period lasted for 7 weeks. Results: Our results showed that the laying rate was improved in the test group at each week of trial (p<0.01), and the levels of estradiol in serum and prolactin in ovary were increased compared with the control group (p<0.05). Conclusion: Based on above results, Enteromorpha powder supplementation at 3% could promote reproductive performance during the late laying period of Zi geese. Objective: The aim of this study was to investigate the effects of Enteromorpha powder supplementation on reproduction-related hormones and genes in the late laying period of Zi geese.Methods: A total of 312 (1-year-old) Zi geese with similar laying rate were randomly divided into 2 groups with 6 replicates each, each with 21 female geese and 5 male geese. The control group was fed with a basal diet and the test group was fed with a diet containing 3% <i>Enteromorpha</i> powder. The trial period lasted for 7 weeks.Results: Our results showed that the laying rate was improved in the test group at each week of trial (p<0.01), and the levels of estradiol in serum and prolactin in ovary were increased compared with the control group (p<0.05).Conclusion: Based on above results, <i>Enteromorpha</i> powder supplementation at 3% could promote reproductive performance during the late laying period of Zi geese.

Characterization of two novel γ-gliadin genes encoded by K genome of Crithopsis delileana and evolution analysis with those from Triticeae

Zhi-Fu Guo,Li-Jun Zhang,Ming Zhong,Yu-Ming Wei,Li Zhang,Hui Ma,Hao-Ge Li,Li-Jing Chen,Jing-Wei Lin,You-Liang Zheng 한국유전학회 2010 Genes & Genomics Vol.32 No.3

By acid polyacrylamide gel electrophoresis (A-PAGE) analysis,it was indicated that the electrophoresis mobility of gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14,KK) had obvious difference with those from common wheat in α, γ and ω region. Using homologous primers, two γ-gliadin genes (gli-Kr1 and gli-Kr2) were isolated from C. delileana,which had been deposited in the GenBank under accession numbers EU283818 and EU283821, respectively. Two γ-gliadin genes of C. delileana had the similar primary structures to the corresponding gene sequences from other wheat related species. The differences were mainly resulted from substitutions,insertions and deletions involving single amino acid residues or motifs of γ-gliadins. The repetitive domains of gli-Kr1 and gli-Kr2 from C. delileana are shorter than most of other sequences. By the alignment of γ-gliadin genes from A, B, D, Am, Au, S, Sl, Ssh, Ss and Sb genomes of Triticum and Aegilops, R genome of Secale (γ-secalin), Ee genome of Lophopyrum and K genome of Crithopsis in Triticeae, phylogenetic analysis indicated that two γ-gliadin genes of C. delileana could be clustered together with a γ-gliadin genefrom Ssh genome of Aegilops by an interior paralleled branch. It was the first time that the γ-gliadin genes encoded by K genome of C. delileana were characterized. These could offer precious information for better understanding the qualities associated with gliadins, the response in coeliac disease and studying the evolutionary relationship of gliadins in Triticeae.

CD4⁺, IL17 and Foxp3 Expression in Different pTNM Stages of Operable Non-small Cell Lung Cancer and Effects on Disease Prognosis

Zhang, Guo-Qing,Han, Feng,Fang, Xin-Zhi,Ma, Xiao-Mei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: To investigate the effects of $CD4^+$, IL17 and Foxp3 expression on prognosis of operable non-small cell lung cancer (NSCLC) with different pTNM stages. Methods: Expression of $CD4^+$, IL17 and Foxp3 in 102 cases of NSCLC tissues and adjacent cancer tissues was detected by immunohistochemistry and associations with prognosis with different pTNM stages were analyzed. The Chi-square test was used to compare count data. Survival differences were evaluated by Kaplan-Meier single factor analysis and the COX regression model was used to analyze the relationship between influential factors and the disease prognosis. The significance level was ${\alpha}$=0.05. Results: Expression of CD4, IL-17 and Foxp3 significantly varied in different pTNM stages of NSCLC tissues (P < 0.05). The same was true for CD4 expression (P < 0.05). The median survival time (MST) in the positive CD4 expression group was evidently higher than that in the negative group (25.8/23.9 months). Compared with stage III, the MST difference of stages I and II in the positive CD4 expression group were statistically significant (P < 0.05). The MST in positive IL-17 and Foxp3 expression groups was obviously lower than that in the corresponding negative group (P < 0.05) (25.6/35.1 months and 24/35.3 months, respectively). There was a significant difference of MST between any two of three stages of positive IL-17 expression group (P < 0.05), and it was the same with positive Foxp3 expression group. TNM stage, negative CD4 expression, and positive IL-17 and Foxp3 expression were the main risk factors for the prognosis of NSCLC. Conclusion: Surgical prognosis of NSCLC can be better assessed by the combination of clinical staging and expression of IL17 and Foxp3.

Recent developments in the field of dehydration of bio-renewable glycerol to acrolein over molecular sieve catalysts

Tianlin Ma,Manyun Yin,Chunyang Su,Ningfei Guo,Xiangxiang Huang,Zhen Han,Yujie Wang,Gangling Chen,Zhi Yun 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

The surplus production of glycerol as a byproduct of biodiesel has become an urgent problem for the sustainabilityof the biodiesel industry. The catalytic transformation of glycerol to value-added chemicalshas attracted the attention of researchers worldwide over the last decade. Among them, selective dehydrationof glycerol to acrolein has gained interest from both academic researchers and industrial applications. Molecular sieves have been considered as the most promising catalysts for industrialapplications of glycerol dehydration. However, there is no systematic summary for the research carriedout for this reaction over molecular sieve catalysts. This work provides a comprehensive and criticalreview of glycerol dehydration to acrolein over molecular sieve catalysts, including ZSM-5, ZSM-11,MCM-22, MCM-41, SBA-15, SAPO-34, HY and Hb. The key technical problems that restrict its industrialapplication are the easy coking and poor stability of the molecular sieves. In particular, the pore size, porestructure, acid amount, acid strength, acid type and reaction conditions that influence the dehydrationperformance are discussed in detail. Furthermore, the reaction mechanism, reaction kinetics, reactionthermodynamics and future research directions for catalyst design and engineering are also presented. This review will deepen our understanding of the catalytic dehydration of glycerol to acrolein and provideguidance for researchers on the rational design of molecular sieve catalysts.

Effect of Chemical Admixtures on Setting Time, Fluidity and Mechanical Properties of Phosphorus Gypsum Based Self-Leveling Mortar

Zhenzhen Zhi,Jian Huang,Yanfei Guo,Siwen Lu,Baoguo Ma 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.5

Phosphorus Gypsum (PG) based Self-Leveling mortar (GSL) is a green and efficient material for floor construction. Its hydration kinetics, rheology property and their effect on mechanical strength of hydrated GSL were crucial to its final application. This study analyzed the effect on setting time of three retarders, namely Protein Salt (PS), Citric Acid (CA), and Sodium Tripolyphosphate (STPP). The fluidity of GSL was investigated individually using three different types of superplasticizer, namely naphthalene (FDN), polycarboxylate (PCE) and melamine (MSF). Finally, the effect of these chemical admixtures on mechanical properties of GSL hardened was examined. Results showed that the optimal contents of PS were 0.1%-0.15% by weight. With 0.1% PS, setting time of GSL retarded to 80min. To reach a maximum fluidity of GSL to 350mm, the optimal contents of PCE, MSF and FDN were 0.2%, 0.6% and 1.0% respectively and corresponding compressive strength of GSL were 54.25MPa, 53MPa and 52.25MPa. Suggesting PCE is the most effective dispersant in PG based GSL system.

A Nonlinear Model-based Variable Impedance Parameters Control for Position-based Impedance Control System of Hydraulic Drive Unit

Kaixian Ba,Guo-liang Ma,Bin Yu,Zheng-guo Jin,Zhi-peng Huang,Jun-xiao Zhang,Xiangdong Kong 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.7

In this paper, aimed at the problem of control accuracy when the traditional position-based impedance control is applied in the hydraulic drive unit (HDU) of legged robot, a kind of nonlinear model-based variable impedance parameters controller (MVIPC) is designed. First, the mathematical model of position-based impedance control for HDU is given. Second, the performance of traditional position-based impedance control is tested on the HDU performance test platform under different working conditions, and the experimental results show that the control accuracy of this control method needs to be improved greatly. Thirdly, the control idea of MVIPC is described, and the theoretical derivation is deduced. MVIPC considers the high-order dynamic characteristics of servo valve, pressure-flow nonlinearity of servo valve, oil compressibility and load characteristics. Finally, the control performance of MVIPC is verified on the HDU performance test platform. The experimental results show that MVIPC can significantly improve the performance of traditional position-based impedance control, and have an excellent adaptability under different working conditions. This research can provide an underlying control method of hydraulic systems during the robot locomotion.

Effect of sodium sulfite, tartaric acid, tannin, and glucose on rheological properties, release of aroma compounds, and color characteristics of red wine

Hao Wang,Zhi-Jing Ni,Wen-Ping Ma,Chang-Bing Song,Jian-Guo Zhang,Kiran Thakur,Zhao-Jun Wei 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2

In this study, we evaluated the effect of additionof non-volatile compounds (sodium sulfite, tartaric acid,tannin, and glucose) on the rheological properties, releaseof aroma compounds, and color of the red wine. Whiledetermining the rheological properties of the supplementedsamples, non-Newtonian fluidic and shear-thinningbehavior of samples was noticed. The viscosity of thesesamples was found in negative correlation with the dose ofaddition of various non-volatile substances. The aromaprofile of red wine after additions showed the change in therelease of the nine key aroma compounds. Among themethyl hexanoate, phenylethyl alcohol, octanoic acid, diethylsuccinate, and ethyl octanoate were profoundly increased. Further, the color of red wines was improved in the presenceof tartaric acid and tannin. Overall, supplementationof various substances during storage period of three differentwines could enormously affect the sensory characteristicsin a dose dependent manner.