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Radiation Hybrid Mapping and mRNA Expression of Chicken N- myc downregulated gene 4
Yong Tian,Li Zhi Lu,Yan Fu,Ai Ping Yuan,Guo Qin Li,Qing Yan Yuan,Zheng Rong Tao,Jin Zhao 한국유전학회 2007 Genes & Genomics Vol.29 No.4
N-myc downregulated gene 4 (NDRG4) is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscle cells. To map NDRG4 gene in chicken chromosome, a 6,000 rads chicken- hamster radiation hybrid panel was used. Primers were designed according to the published human sequence for amplification of chicken NDRG4. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken NDRG4, and found that the assembled contig shared a high percentage of similarity with that of human gene. PCR of samples from ChickRH6 revealed the locations of NDRG4 to be linked to the maker PARD6G (5 cR away) with a LOD score 20.46. In addition, we detected the mRNA expression and distribution of chicken NDRG4 in various tissues by RT-PCR, and found that NDRG4 was highly expressed in chicken brain and heart, whereas lowly but detectable in thymus. The mRNA expression of this gene in chicken liver, spleen, lung and muscle was rarely detectable under present experimental conditions.
Two New Phenolic Glycosides from Curculigo orchioides
Ai-Xue Zuo,Yong Shen,Zhi-Yong Jiang,Xue-Mei Zhang,Jun Zhou,Jun Lü,Ji-Jun Chen 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.3
Two new phenolic glycosides were isolated from the rhizomes of Curculigo orchioides Gaertn.. Based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (COSY, HSQC, and HMBC), their structures were elucidated as 3-hydroxyl-5-methyphenol-1-O-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside (1) and 1',3'-dimethoxyl-4-hydroxyalangifolioside (2).
Two New Diterpenoid Alkaloids from Aconitum brachypodum
Yong Shen,Ai-Xue Zuo,Zhi-Yong Jiang,Xue-Mei Zhang,Hong-Ling Wang,Ji-Jun Chen 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11
Two new diterpenoid alkaloids, N(19)-en-denudatine (1) and N(4)-butanone-flavaconitine (2), were isolated from Aconitum brachypodum Diels.. Their structures were elucidated by comprehensive spectroscopic analyses including UV, IR, MS, 1D- and 2D-NMR.
Two New Diterpenoid Alkaloids from Aconitum brachypodum
Shen, Yong,Zuo, Ai-Xue,Jiang, Zhi-Yong,Zhang, Xue-Mei,Wang, Hong-Ling,Chen, Ji-Jun Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.11
Two new diterpenoid alkaloids, N(19)-en-denudatine (1) and N(4)-butanone-flavaconitine (2), were isolated from Aconitum brachypodum Diels.. Their structures were elucidated by comprehensive spectroscopic analyses including UV, IR, MS, 1D- and 2D-NMR.
Two New Phenolic Glycosides from Curculigo orchioides
Zuo, Ai-Xue,Shen, Yong,Jiang, Zhi-Yong,Zhang, Xue-Mei,Zhou, Jun,Lu, Jun,Chen, Ji-Jun Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.3
Two new phenolic glycosides were isolated from the rhizomes of Curculigo orchioides Gaertn.. Based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (COSY, HSQC, and HMBC), their structures were elucidated as 3-hydroxyl-5-methyphenol-1-O-[${\beta}$-D-glucopyranosyl-($1{\rightarrow}3$)-${\beta}$-D-glucopyranoside (1) and 1',3'-dimethoxyl-4-hydroxyalangifolioside (2).
Fused Polypeptide with DEF Induces Apoptosis of Lung Adenocarcinoma Cells
Liang, Ai-Ling,Zhang, Ting-Ting,Zhou, Ning,Huang, Di-Nan,Liu, Xin-Guang,Liu, Yong-Jun,Tu, Zhi-Guang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12
To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.
Long, Zhi,Gao, Liqin,Li, Yankai,Kang, Baotao,Lee, Jin Yong,Ge, Junjie,Liu, Changpeng,Ma, Shuhua,Jin, Zhao,Ai, Hongqi American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.44
<P>The self-assembly powder (SAP) with varying Nafion content was synthesized and characterized by XRD, XPS, HRTEM, and mapping. It is observed that the oxygen from oxygen functional groups transfers to the surface of Pt and generate PtO during the process of self-assembly with the mechanism of micro galvanic cell, where Pt, carbon black, and Nafion act as the anode, cathode and electrolyte, respectively. The appearance of PtO on the surface of Pt leads to a turnover of Nafion structure, and therefore more hydrophilic sulfonic groups directly contact with Pt, and thus the triple-phase boundary (TPB) has been expanded.</P>
The Domain Ontology and Domain Rules Based Requirements Model Checking
LI Zong-yong,WANG Zhi-xu,ZHANG Ai-hui,Xu Yong 보안공학연구지원센터 2007 International Journal of Software Engineering and Vol.1 No.1
Many ontology-based methods have been proposed and applied in order to elicit system requirements correctly and unambiguously. However, most of ontologies in these methods are purely conceptual models. Furthermore, the domain knowledge base only captures domain concepts and neglects domain-restricted rules. If the requirements model violate these rules or contradict the usual business behavior, they become unreasonable. This paper suggests a formal approach to precisely describe ontology using description logic at first, and then model the integrity rules and derivation rules which restrict the business behavior. All the rules are represented in three aspects: syntax, semantics and visualization. Finally, the requirements model checking framework is provided combining domain ontology and domain rules, which makes the requirements elicitation process both guided by domain ontology and restricted by domain rules. Therefore, the acquired requirements would comply with both business needs and domain knowledge.
Wei Li,Yong Liu,Jiang-Wei Zhang,Chun-Zhi Ai,Nan Xiang,Hui-Xin Liu,Ling Yang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.1
Treatment of androgen-independent prostate cancer (AIPC) remains unsatisfactory. In our present experiment, natural occurring ginsenosides (NOGs) and intestinal bacterial metabolites (IBMs) were employed to investigate their anti-AIPC cell growth activity using PC-3 cells. Our results showed that the IBMs exerted more portent anti-AIPC activity than NOGs, by decreasing survival rate, inhibiting proliferation, inducing apoptosis, and leading to cell cycle arrest in AIPC PC-3 cells. The increase of LogP and decrease of C-6 steric hindrance, which were caused by deglycosylation by intestinal bacteria, may be the reason for the higher anti-AIPC activity of IBMs.
Transient Thermal Response of a Multilayered Geomaterial Subjected to a Heat Source
Lu Jun Wang,Zhi Yong Ai 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.9
The analysis of layered geomaterials thermo-mechanical coupling response is important in design and analysis of a nuclear waste repository. Conventional analytical approaches usually have some intrinsic faults of ill-conditioned matrices for thick layers or accumulative numerical errors for a great quantity of layers for containing positive exponential functions. This paper presents a stable computational approach with no positive exponential functions to study the transient thermal responses of a layered geomaterial subjected to a heat source. Based on the governing equations of thermo-mechanical coupling problems, the analytical layer elements for a finite layer and a half–space are derived by utilizing Laplace and Hankel transforms. The analytical layer elements are then assembled into the total stiffness matrix and solved in the transformed domain. Finally, the Laplace–Hankel transform inversion is employed to obtain an actual solution in the physical domain. Numerical computations indicate that the difference of geomaterial mass properties between layers shows obvious effects on thermal response of the layered system, and these results can be served as a benchmark solution for future analyses of stratified material.