pH-Sensitive Polypeptide Conjugated with Carborane Clusters and Cyanine for NIR Bioimaging and Multi-Therapies

Zheng Ruan,Pan Yuan,Titao Jing,Tao Xing,Lifeng Yan 한국고분자학회 2018 Macromolecular Research Vol.26 No.3

A novel pH-sensitive carborane and cyanine dye conjugated polypeptide and its micelles has been designed and synthesized for potential near infrared fluorescence imaging-guided boron neutron capture therapy (BNCT). The amphiphilic polymer we synthesized with tertiary amine as functional group could self-assemble to micelle nanoparticles at neutral pH, and responsive to acidic solution for the disassembly of the micelles for drug release. The tertiary amine sensitive induced process was observed via dynamic light scattering (DLS) and transmission electron microscopy (TEM). The efficiency of cell killing ability is studied by MTT assays and fluorescence microscope measurement, indicating a new intelligent system for potential NIR imaging-guided therapy.

Mechanical Properties and Microstructure of Polypropylene Fiber Reinforced Cement Mortar Soil

Bo Ruan,Hui Ding,Jidong Teng,Wei Deng,Shilong Zheng,Chenxi Ruan 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.6

A series of unconfined compressive strength tests and flexural strength tests are carried out to evaluate the improved effect of polypropylene fiber on the defects of cement mortar soil. The following factors, including the fiber content, cement content, sand content and curing age, are studied to investigate the influences on the mechanical properties and microstructure of the samples. The results show that the unconfined compressive strength (UCS), residual strength and flexural strength of the fiber reinforced cement mortar soil (FRCMS) substantially increase with increasing fiber content. The peak strain and ratio of the flexural-compression strength (Rfcs) of the FRCMS first increase and then decrease with an increase in fiber content,and the optimal fiber content is 3.5%. The brittleness index of the FRCMS is found to be inversely proportional to fiber content. The results suggest that the addition of an appropriate amount of fibers can substantially improve the plasticity and lateral stress capacity of the FRCMS. The strength of the FRCMS improves with the increase in cement content, sand content and curing age within a certain range. The microstructure of the FRCMS are analyzedby scanning electron microscopy (SEM) tests.

Serum Proteomics Analysis of Feline Mammary Carcinoma Based on Label-free and PRM Techniques

Jia-San Zheng,Ren-Yue Wei,Zheng Wang,Ting-Ting Zhu,Hong-Ri Ruan,Xue Wei,Kai-Wen Hou,Rui Wu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: Feline mammary carcinoma is the third most common cancer that affects female cats. Objectives: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. Methods: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. Results: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. Conclusions: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.

Deletion of the Lmna Gene Induces Growth Delay and Serum Biochemical Changes in C57BL/6 Mice

Ruan, J.,Liu, X.G.,Zheng, H.L.,Li, J.B.,Xiong, X.D.,Zhang, C.L.,Luo, C.Y.,Zhou, Z.J.,Shi, Q.,Weng, Y.G. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.1

The A-type lamin deficient mouse line ($Lmna^{-/-}$) has become one of the most frequently used models for providing insights into many different aspects of A-type lamin function. To elucidate the function of Lmna in the growth and metabolism of mice, tissue growth and blood biochemistry were monitored in Lmna-deficient mice, heterozygous ($Lmna^{+/-}$) and wide-type ($Lmna^{+/+}$) backcrossed to C57BL/6 background. At 4 weeks after birth, the weight of various organs of the $Lmna^{-/-}$, $Lmna^{+/-}$ and $Lmna^{+/+}$ mice was measured. A panel of biochemical analyses consisting of 15 serological tests was examined. The results showed that Lmna deficient mice had significantly decreased body weight and increased the ratio of organ to body weight in most of tissues. Compared with $Lmna^{+/+}$ and $Lmna^{+/-}$ mice, $Lmna^{-/-}$ mice exhibited lower levels of ALP (alkaline phosphatase), Chol (cholesterol), CR (creatinine), GLU (glucose), HDL (high-density lipoprotein cholesterol) and higher levels of ALT (alanine aminotransferase) (p<0.05). $Lmna^{-/-}$ mice displayed higher AST (aspartate aminotransferase) values and lower LDL (lowdensity lipoprotein cholesterol), CK-MB (creatine kinase-MB) levels than $Lmna^{+/+}$ mice (p<0.05). There were no significant differences among the three groups of mice with respect to BUN (blood urea nitrogen), CK (creatine kinase), Cyc C (cystatin C), TP (total protein), TG (triacylglycerols) and UA (uric acid) levels (p>0.05). These changes of serological parameters may provide an experimental basis for the elucidation of Lmna gene functions.

NI-HF ALLOYS : A NEW BONDING MATERIAL FRO SINGLE CRYSTAL SUPERALLOYS

Yunrong, Zheng,Zhongci, Ruan 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.1 No.2

Hf is a potential melting point depressant in the interlayer alloys used for brazing. The concentration of Hf in the interdendrites in the later stage of solidifcation for Hf-bearing superalloys is more than 10wt%. It may be considered that dendrites formed in the early solidification are brazed by interdendritic Hf-rich melts, therefore, the composition of the melts may be utilized as essential composition for the interlayer alloy. Eventually, the alloy containing Ni-18.6Co-4.5Cr-4.7W-25.6Hf(wt%) has been developed. The melting range of this alloy is $1195-1232^{\circ}C$. Microstructure of the ribbon prepared by the interlayer alloy consists of ${\gamma}+Ni_7Hf_2$ eutectic. The two phases have orientation relationship of $(001)_{Ni7Hf2}//(111)_{\gamma}, [101]_{Ni7Hf2}//[112]_{\gamma}$. Using the above ribbon material, transient liquid-phase(TLP) bonding of $1270^{\circ}C/48h$ was performed. During isothermal solidification of the bond, the content of Al and Ti increases and concentration of Hf decreases. Large amount of ${\gamma}+{\gamma}'$ eutectic formed which gets large quantity of Hf and restrains the formation of Hf-rich intermentallic compound in the bond. As a result, bonding free of Si and B for single crystal superalloys can be realized.

Expression profiling identified IL-8 as a regulator of homotypic cell-in-cell formation

( Banzhan Ruan ),( Chenxi Wang ),( Ang Chen ),( Jianqing Liang ),( Zubiao Niu ),( You Zheng ),( Jie Fan ),( Lihua Gao ),( Hongyan Huang ),( Xiaoning Wang ),( Qiang Sun ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.8

Homotypic cell-in-cell (CIC) structures forming between cancer cells were proposed to promote tumor evolution via entosis, a nonapoptotic cell death process. However, the mechanisms underlying their formation remained poorly understood. We performed a microarray analysis to identify genes associated with homotypic CIC formation. Cancer cells differing in their ability to form homotypic CIC structures were selected for the study. Association analysis identified 73 probe sets for 62 candidate genes potentially involved in CIC formation. Among them, twenty-one genes were downregulated while 41 genes were upregulated. Pathway analysis identified a gene interaction network centered on IL-8, which was upregulated in high CIC cells. Remarkably, CIC formation was significantly inhibited by IL-8 knockdown and enhanced upon recombinant IL-8 treatment, which correlated with altered cell-cell adhesion and expression of adhesive molecules such as P-cadherin and γ-catenin. Together, our work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion. [BMB Reports 2018; 51(8): 412-417]

The Prebiotic Lactosucrose Modulates Gut Metabolites and Microbiota in Intestinal Inflammatory Rats

Xiaoli Zhou,Zheng Ruan,Xiaoliu Huang,Yan Zhou,Shiqiang Liu,Yulong Yin 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1

The effect of lactosucrose on the short chainfatty acid (SCFA) content, the intestinal flora, the acidity,and the amino nitrogen (NH3-N) content of the intestine ininflammatory bowel disease (IBD) rats was investigated. Dietary lactosucose supplementation increased amounts ofbeneficial bacteria and diminished amounts of pathogenicbacteria. Lactosucrose decreased the NH3-N content in cecaland colonic digesta, compared to inflammatory rats. Lactosucrose increased the acidity in the intestinal lumen,and the acetic acid, propionic acid, and butyric acidcontents in cecal and colonic digesta, compared toinflammatory rats. The butyrate content in the lactosucrosegroup was higher than for the inflammatory, sulfasalazine,and normal groups. Lactosucrose promoted beneficialintestinal health and prevented intestinal inflammation.

Chlorogenic Acid Decreased Intestinal Permeability and Ameliorated Intestinal Injury in Rats via Amelioration of Mitochondrial Respiratory Chain Dysfunction

Yan Zhou,Zheng Ruan,Lili Zhou,Yuhui Yang,Shumei Mi,Ze-Yuan Deng,Yulong Yin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1

Chlorogenic acid (CGA), an abundant polyphenol compound in plants, exhibits anti-oxidant effects. The protective effect of CGA in the rat intestine with endotoxin infusion was evaluated. CGA administration ameliorated endotoxin-induced intestinal injury, and decreased the ratio of lactulose/ mannitol, the ileum pathological grade, the myeloperoxidase activity in the ileum, and the malondialdehyde content in the ileum and in ileum mitochondria. The small intestine weight, activities of alkaline phosphatase and superoxide dismutase in the ileum, and β-nicotinamide adenine dinucleotide reduce form (NADH) dehydrogenase and succinate dehydrogenase activities in ileum mitochondria were increased. Intestinal permeability was positively correlated with intestinal mitochondrial injury indicated as the level of malondialdehyde in ileum mitochondria, and negatively correlated with NADH dehydrogenase activity. Dietary administration of CGA protected against increased intestinal permeability caused by endotoxin infusion. The protective effect of CGA was probably associated with a decrease in mitochondrial lipid peroxidation levels and an increase in NADH dehydrogenase activity.

Genome-wide association study for loin muscle area of commercial crossbred pigs

Luan Menghao,Ruan Donglin,Qiu Yibin,Ye Yong,Zhou Shenping,Yang Jifei,Sun Ying,Ma Fucai,Wu Zhenfang,Yang Jie,Yang Ming,Zheng Enqin,Cai Gengyuan,Huang Sixiu 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

Objective: Loin muscle area (LMA) is an important target trait of pig breeding. This study aimed to identify single nucleotide polymorphisms (SNPs) and genes associated with LMA in the Duroc×(Landrace×Yorkshire) crossbred pigs (DLY). Methods: A genome-wide association study was performed using the Illumina 50K chip to map the genetic marker and genes associated with LMA in 511 DLY pigs (255 boars and 256 sows). Results: After quality control, we detected 35,426 SNPs, including six SNPs significantly associated with LMA in pigs, with MARC0094338 and ASGA0072817 being the two key SNPs responsible for 1.77% and 2.48% of the phenotypic variance of LMA, respectively. Based on previous research, we determined two candidate genes (growth hormone receptor [GHR] and 3-oxoacid Co A-transferase 1 [OXCT1]) that are associated with fat deposition and muscle growth and found further additional genes (MYOCD, ARHGAP44, ELAC2, MAP2K4, FBXO4, FBLL1, RARS1, SLIT3, and RANK3) that are presumed to have an effect on LMA. Conclusion: This study contributes to the identification of the mutation that underlies quantitative trait loci associated with LMA and to future pig breeding programs based on marker-assisted selection. Further studies are needed to elucidate the role of the identified candidate genes in the physiological processes involved in LMA regulation.

Metagenomic and Proteomic Analyses of a Mangrove Microbial Community Following Green Macroalgae Enteromorpha prolifera Degradation

( Yijing Wu ),( Chao Zhao ),( Zheng Xiao ),( Hetong Lin ),( Lingwei Ruan ),( Bin Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Twodimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple “omics” analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.