Synergistic Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma in Streptozotocin-Induced Diabetic Rats

( Zhen Zhen Lian ),( Xiao Jing Yin ),( Hua Li ),( Li Li Jia ),( Xiu Zhen He ),( Yong Bo Yan ),( Nai Hua Liu ),( Ka Yiu Wan ),( Xiao Kun Li ),( Shao Qiang Lin ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.1

Background: Diabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear. Objective: We investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model. Methods: Forty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery. Results: Wound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-β1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls. Conclusion: The combination of BMSCs and PRP aids diabetic wound repair and regeneration. (Ann Dermatol 26(1) 1∼10, 2014)

Engineering Mathematics Teaching Strategy Based on Cooperative Learning

Wan Zhen Zhu 한국수학교육학회 2010 수학교육연구 Vol.14 No.1

2-ORDER TURBULENCE CLOSURE PLANKTON ECOSYSTEM DYNAMICS MODEL AND ITS APPLICATION

Zhen Wen Wan,Ye Li Yuan,Fang Li Qiao 한국해안해양공학회 1999 학술강연회 발표논문초록집 Vol.1 No.1

The basic concepts of continuous neurogen dynamics, particle and continuous mass, were cited to describe plankton ecosystems, so a continuous neurogen dynamics model of plankton ecosystem was developed by logically reasoning with less ecological experiential relation. For precise parameterization of diffusion acted on plankton by hydrodynamics, the scheme of 2-order turbulence closure of geophysical fluid dynamics was generalized to enclose the equations of plankton ecosystem dynamics model. This model was applied to simulate a natural ecological process in Jiaozhou Bay. Comparison between simulation and data showed the exciting prognosis ability of this model.

Markers in Morphine- and Cocaine-Addicted Animals

( Ki Wan Oh ),( Eun Jeong Kim ),( Hyoung Chun Kim ),( Sei Kwan Oh ),( Choon Gon Jang ),( Chae Ha Yang ),( Kwang Soon Park ),( Jin Yi Han ),( Zhen Zhen Hu ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.1

These experiments were designed to use typical makers from behaviors and molecular basis in addicted animals of morphine and cocaine. Morphine has been widely abused with a high physical dependence liability. Morphine withdrawal activates the intracellular CAMP signaling pathway and further leads to changes in the expression of the CAMP response element binding protein (CREB), which may be important to the development and expression of morphine dependence. From these experiments, repeated morphine (10 mg/kg, twice per day for 7 days) developed physical dependence. Withdrawal signs were precipitated by naloxone and also increased the expression of the CREB. In addition, repeated exposure of cocaine (15 mg/kg) to mice develops locomotor sensitization and produced lasting behavioral sensitivity. Cocaine- and amphetamine-regulated transcript peptide (CART) peptide was up-regulated by repeated administration of cocaine in the striatum. Therefore, repeated morphine induced the development of physical dependence and increased PCREB. In addition, repeated cocaine induced locomotor sensitization and over-expressed CART peptide. In conclusion, the development of physical dependence and PCREB for morphine, and locomotor sensitization and CART peptide over-expression for cocaine would be useful markers to predict the abuse potential of opioid analgesics and pychostimulant drugs in animals, respectively

17세기 한중 실학자의 교육개혁안 비교연구 -유형원과 황종희를 중심으로

만진초 ( Wan Zhen-chao ) 한국실학학회 2017 한국실학연구 Vol.0 No.33

본 논문은 17세기 한중양국의 유명한 사상가 유형원(柳馨遠)과 황종희(黃宗羲) 각각의 대표작인 『반계수록(磻溪隨錄)』과 『명이대방록(明夷待訪錄)』을 중심으로 두 사람이 구상한 교육개혁방안을 비교하여 17세기 동아시아 국제정세 격동기에 한중양국 사상활동의 특징을 밝히려고 시도한다. 논문은 우선 유형원과 황종희 각각의 생애, 저서활동, 그리고 『반계수록』과 『명이대방록』의 내용을 정리하기를 통해 두 사람은 살았던 역사적인 환경은 두사람의 개혁사상에 미치는 영향과 그들이 구상하는 이상 사회의 모습을 설명하려고 한다. 다음 필자가 교육제도개혁과 인재선발제도 두 측면에 초점을 맞춰『반계수록』과 『명이대방록』에서 각각 그리는 교육개혁방안을 분류하고 정리한 다음에 두 사람의 교육개혁방안에 대한 비교를 통해 그들의 교육개혁의 비슷함과 차이점을 논술한다. 유형원의 교육개혁의 핵심은 인재의 덕행을 중요시하는것은 중심으로 두어 체계화된 교육체제와 인재선발제도를 구축하는 것과 달리, 황종희의 교육개혁의 핵심은 학교의 정치에 참여하여 정무를 논의하는 기능을 강화시키고, 관진엄출(寬進嚴出)의 인재선발제도를 채택하여 군주전제를 제어하려는 것이다. 마지막으로 상술한 정리와 분석을 통해 결론을 내린다. 유형원과 황종희는 같은 시기(조선왕조의 중후기와 명청교체기)에 다른 나라에서 살았지만, 두 사람은 다 유가경전에 직접 회귀하는 것으로 당시 형이상학적인 주자학의 속박에서 벗어나려고 시도하였다. 그들은 경세치용(經世致用)의 시각으로 각각의 나라가 품고 있는 문제점들을 살피며 각자의 개혁방안을 제기하였다. Based on the Bangye-Surok(磻溪隨錄) and Ming-yi Dai-fang Lu(明夷待訪錄), written by Yu Hyeong-won(柳馨遠, 1622~1673) and Huang tsung-hsi(黃宗羲, 1610~1695) the two prominent thinkers in South Korea and China in the 17^th century, This study tries to reveal the characteristics of South Korea and China`s ideological activities in a period of the historical structure`s upheaval in East Asia, by comparing the education reform plans conceived by the two people. This study, first, tries to explain the influence of the historical environment they lived in on the two people`s reform thoughts, and the features of the ideal society they envisaged, by arranging their career, writing activities and the content of the Bangye-Surok and Ming-yi Dai-fang Lu, severally. And then this study sorts out the education reform plans delineated in the Bangye- Surok and Ming-yi Dai-fang Lu from the fellow two aspects, which is the reform of the educational system and the selection system of talented people. By comparing their education reform plans, this study discusses the similarities and differences of the two people`s education reform, and points out that the core of Yu Hyeong-won`s education reform is to constructing systematic education system and selection system by emphasizing the importance of talents` moral conduct, but Huang tsung-hsi wanted to achieve the purpose of limiting the autocratic monarchy through strengthening the function of the schools` political participation and adopting the “easy in and strict out” selection system. Finally, the conclusion is drawn through the above analysis. Although Yu Hyeong-won and Huang tsung-hsi were the prominent thinkers who lived in different countries in the same period, but they both tried to directly return to Confucian classics(儒敎經典), and slip the leash of the metaphysical Zhuzi`s Theory(朱子學). With the statecraft viewpoint to re-analyze the problems which their countries suffered, they brought forward their own reform plans.

The BRAF^T1799A Mutation is not Associated with Occult Contralateral Carcinoma in Patients with Unilateral Papillary Thyroid Microcarcinoma

Wan, Han-Feng,Zhang, Bin,Yan, Dan-Gui,Xu, Zhen-Gang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Background: The phenomenon of occult carcinoma maybe observed in patients with clinically unilateral papillary thyroid microcarcinoma (PTMC). Although many studies have reported that the $BRAF^{T1799A}$ mutation is associated with aggressive PTMC, the relationship between $BRAF^{T1799A}$ mutation and occult carcinoma is unclear. The aim of this study was to investigate the risk factors, including $BRAF^{T1799A}$ mutation, for occult contralateral carcinoma in clinically unilateral PTMC accompanied by benign nodules in the contralateral lobe. Materials and Methods: From January 2011 to December 2013, we prospectively enrolled 89 consecutive PTMC patients with clinically unilateral carcinoma accompanied by benign nodules in the contralateral lobe who received a total thyroidectomy and cervical lymph node dissection. $BRAF^{T1799A}$ mutation was tested by pyrosequencing on postoperative paraffin specimens. The frequency and predictive factors for occult contralateral carcinoma were analyzed with respect to the following variables: age, gender, family history, tumor size, presence of Hashimoto thyroiditis, extrathyroidal extension, central lymph node metastasis, multifocality of primary tumor, or $BRAF^{T1799A}$ mutation. Results: A total of 36 patients (40.4%) had occult PTMC in the contralateral lobe. The median diameter of the occult tumors was $0.33{\pm}0.21cm$. The $BRAF^{T1799A}$ mutation was found in 38 cases (42.7%). According to the univariate analysis, there were no significant differences between the presence of occult contralateral carcinoma and age, gender, family history, tumor size, presence of Hashimoto thyroiditis, extrathyroidal extension, central lymph node metastasis, multifocality of primary tumor, or $BRAF^{T1799A}$ mutation. Conclusions: Using current methods, it is difficult to preoperatively identify patients with PTMC, and further research is needed to determine predictive factors for the presence of occult contralateral carcinoma in patients with unilateral PTMC.

Being Adaptive to Pain Enhances Sham Acupuncture Analgesia: A Crossover Healthy Human Study

Zhen Zheng,Dawn Wong Lit Wan,Lars Arendt-Nielsen,Dong Y. Yao,Genevieve Iversen,Charlie C. Xue,Kelun Wang 사단법인약침학회 2017 Journal of Acupuncture & Meridian Studies Vol.10 No.6

We have reported a model that distinguishes pain adaptive individuals (PA) from those who are pain non-adaptive (PNA). The present randomised, cross-over, participant-assessor blinded study aimed to determine the impact of pain adaptability on individuals’ response to real and sham acupuncture. Healthy volunteers (nine PA and 13 PNA) were randomly allocated to receive real and sham acupuncture on the left hand and forearm in two separate acupuncture sessions. Pressure pain thresholds (PPTs) were measured at bilateral forearms and right leg before, immediately after and 20 minutes after the end of acupuncture. Ratings to pinprick and suprathreshold PPT were also recorded. The two groups were comparable in their demographic and baseline data. Analgesia induced by real or sham acupuncture did not differ on any outcome measures. PA responded to acupuncture needling better than PNA, and to sham needling (20% increase in PPT) better than to real acupuncture (7.9%). Those differences were at 20 min after end of acupuncture in the areas distant to the needling sites. PNA reported little changes in PPT. Being adaptive to pain was associated with enhanced distant analgesia in response to sham acupuncture. Our finding might partly explain varied acupuncture analgesia in clinical practice and trials.

Strength criterion of plain recycled aggregate concrete under biaxial compression

Zhen-Jun He,Gan-Wen Liu,Wan-Lin Cao,Chang-Yang Zhou,Zhang Jia-Xing 사단법인 한국계산역학회 2015 Computers and Concrete, An International Journal Vol.16 No.2

This paper presents results of biaxial compressive tests and strength criterion on two replacement percentages of recycled coarse aggregate (RPRCA) by mass for plain structural recycled aggregate concrete (RAC) at all kinds of stress ratios. The failure mode characteristic of specimens and the direction of the cracks were observed and described. The two principally static strengths in the corresponding stress state were measured. The influence of the stress ratios on the biaxial strengths of RAC was also analyzed. The experimental results showed that the ratios of the biaxial compressive strength  3f to the corresponding uniaxial compressive strength c for the two RAC are higher than that of the conventional concrete (CC), and dependent on the replacement percentages of recycled coarse aggregate, stress states and stress ratios; however, the differences of tensile-compressive ratios for the two RAC and CC are smaller. On this basis, a new failure criterion with the stress ratios is proposed for plain RAC under biaxial compressive stress states. It provides the experimental and theoretical foundations for strength analysis of RAC structures subject to complex loads.

Spillover Effects of FDI Inflows on the Banking Industry in China

Jung Wan Lee,Zhen Wang 한국유통과학회 2017 한국유통과학회 학술대회 논문집 Vol.2017 No.-

The study examines the magnitude of economic spillover and the impact of foreign direct investment (FDI) inflow on the efficiency of the bank management in China This study examines direct and indirect spillover in the short-run and their economic spillover in the long-run. Unit root tests, cointegration tests, vector error correction models, and Wald tests are employed in the empirical analysis using monthly time series data from January 2002 to September 2013 retrieved from the Bank of China database. In testing long-run economic spillover, this study finds that FDI inflows have a significant economic spillover, but the spillover is likely to be negative. In testing short-run economic spillover, this research finds that FDI inflows have a significant positive direct effect on the efficiency of the bank management in Chain.

Spillover Effects of Foreign Direct Investment Inflows and Exchange Rates on the Banking Industry in China

Jung Wan Lee,Zhen Wang 한국유통과학회 2018 The Journal of Asian Finance, Economics and Busine Vol.5 No.2

The study examines the magnitude of economic spillover and the impact of foreign direct investment (FDI) inflows on the efficiency of the bank industry in China. This study employs unit root tests, cointegration tests and cointegrating regression analysis, including fully modified ordinary least squares (FMOLS), canonical cointegrating regression (CCR) and dynamic OLS (DOLS) to test the proposed hypotheses. The sample is restricted to the period of time in which monthly data is available and comparable among variables for the period from January 2002 to October 2013 (142 observations). All of the time series data was collected and retrieved from the People’s Bank of China, China Monthly Statistics from the National Bureau of Statistics of China, and International Financial Statistics database from International Monetary Fund. The results of the Johansen cointegration test suggest that there is a long-run equilibrium relationship between FDI inflows, foreign exchange rate and banks performance in China. The results of cointegrating regression analysis using FMOLS, CCR and DOLS suggest that M2 supply and FDI inflows are significant at the 0.01 level. The results confirm that FDI inflows in the banking sector are positively related to the increase of banks productivity and performance and short-term loans in China. However, the results suggest that Chinese Yuan currency exchange rate to U.S. dollar is not significant in the banking and financial industry of China.