Adaptation of murine anaplastic thyroid cancer cell line from hybrid to inbred C57BL/6 mice

Zhen Xu,Seong Yun Ha,Hyo Shik Shin,Minjun Kim,Su-jin Kim,Jae-Kyung Won,Seong-Hyeon Kim,Sun Wook Cho,Kyu Eun Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Background: Immuno-competent mouse model is indispensable for the study of tumor immune microenvironment. TBP-3743 cell line is derived from anaplastic thyroid cancer arising in immune-competent B6129SF1 mouse with thyroid-specific BrafV600E/WT and deletion of Trp53. Aim of this study is to adapt TBP-3743 cell line to C57BL/6 stain, widely used mouse strain which has same MHC haplotype with that of B6129SF1. Methods: TBP-3743 cell line was subcutaneously injected into C57BL/6 mice. The mouse with the rapidest tumor growth was sacrificed for primary culture and the cultured cells were injected into naive C57BL/6 mice. This serial of processes, called adaptation, has been performed three times. The concentration-dependent growth analysis is performed by comparing the tumor growth of 1x107-injected and 5x106-injected group. Furthermore, Original TBP-3743 cells and adapted TBP-3743 cells were treated with BRAFV600E inhibitor(PLX-4032, 1uM) in vitro and cell proliferation was measured by luminometric detection. For Immunotherapy, mice were treated 10mg/kg of PLX-4032 on daily basis, 15mg/kg of anti-CD47 every other day, 10mg/kg of anti-PD-L1 every other day Results: In the course of adaptation, the lag phage of tumor growth shortened and tumor growth rate had dramatically increased. In vitro growth of Both TBP-3743 and adapted TBP-3743(TBP-3743B6) were effectively BRAFV600E-selective inhibitor (PLX-4032). In vivo treatment of PLX-4032 with anti-CD47 and/or anti-PD-L1 reduced tumor volume in mice injected with TBP-3743B6 and prolonged their survival compared to control Conclusions: We established a novel immunocompetent murine anaplastic thyroid cancer model available in C57BL/6 inbred mice. TBP-3743B6 grows rapidly and stably in immunocompetent C57BL/6 mice. This mouse model can save unnecessary time, cost and labor for the research of tumor immune microenvironment. Also, this newly established cell line will be a useful resource to expand the feasibility of basic research as well as drug screening for potential therapeutic effects on anaplastic thyroid cancer.

Isolation and Characterization of a cDNA Encoding Two Novel Heat-shock Factor OsHSF6 and OsHSF12 in Oryza Sativa L.

Jin Ge Liu,Quan Hong Yao,Zhen Zhang,Ri He Peng,Ai Sheng Xiong,Fang Xu,Hong Zhu 한국생활환경학회 2005 BMB Reports Vol.38 No.5

원격탐사자료를 이용한 인공구조물 건설에 의한 군산 유부도 조간대의 지형변화 및 표면특성에 관한 연구

( Zhen Xu ),김덕진 ( Duk Jin Kim ),김승희 ( Seung Hee Kim ) 대한원격탐사학회 2013 大韓遠隔探査學會誌 Vol.29 No.1

20세기 후반부터 유부도 주위에서 하구언 축조 등 비교적 큰 규모의 간척사업들이 진행되었다. 이러한 간척사업의 시작으로 인공구조물들이 연안에 축조됨에 따라 주변 해역의 해류, 조위 및 조류의 변화를 야기하여 퇴적 및 침식으로 인한 연안지형의 변화가 예상된다. 따라서 이 연구에서는 유부도 조간대를 포함하는 Landsat TM/ETM+자료를 1998년부터 2012년까지 획득한 후 waterline 기법을 적용하여 인공구조물 축조에 의한 지형변화를 탐지하였다. 그 결과 유부도의 동쪽, 북동쪽, 서쪽 조간대에서 주로 퇴적이 일어났으며 그 면적이 약 4.5 km2 이상 되었다. 한편, RADARSAT-2의 완전편파 SAR자료에 Freeman-Durden decomposition을 적용하여 퇴적이 뚜렷이 일어난 지역에 대한 산란특성을 분석하였다. 그 결과 동쪽 퇴적지역은 서쪽 및 북동쪽의 퇴적지역과는 다른 산란특징이 관찰되었으며, 이는 퇴적환경의 차이로 인해 다른 종류의 퇴적물이 퇴적되어 발생한 것으로 여겨진다. Large-scale coastal construction projects, such as land reclamation and dykes, were constructed from the late twentieth century in Yoobu-Do region. Land reclamation combined with the dynamics of tidal currents may have accelerated local sedimentation and erosion resulting in rapid reformation of coastal topography. This study presents the results of the topography changes around Yoobu-Do by large-scale coastal constructions using time-series waterline extraction technique of Landsat TM/ETM+data acquired from 1998 to 2012. Furthermore, the Freeman-Durden decomposition was applied to fully polarimetric RADARSAT-2 SAR data in order to analyze the scattering mechanisms of the deposited surface. According to the case study, the deposition areas were over 4.5 km2 and distributed in the east, northeast, and west of Yoobu-Do. In the eastern deposition area, it was found that the scattering mechanism was difference from other deposition areas possibly indicating that different types of soil were deposited.

Characterization of a Novel cry1-Type Gene from Bacillus thuringiensis subsp. alesti Strain LY-99

Xu Feng Qi,Ming Shun Li,Jae Young Choi,Jong Yul Roh,Ji Zhen Song,Yong Wang,Byung Rae Jin,Yeon Ho Je,Jian Hong Li 한국잠사학회 2009 International Journal of Industrial Entomology Vol.18 No.1

B. thuringiensis strain LY-99 belonging to subsp. Alesti (H3a3c), was isolated from Chinese tobacco warehouse and showed significantly high toxicity to Plutella xylostella. For the identification of the cry1-type genes from B. thuringiensis LY-99, an extended multiplex PCR-restriction fragment length polymorphism (PCR-RFLP) method was established by using two pairs of universal primers based on the conserved regions of the cry1-type genes to amplify around 2.4 kb cry1-type gene fragments. Then the DNA fragment was cloned into pGEM-T Easy vector and digested with EcoRI and EcoRV enzymes. Through this method, a known cry1-type gene was successfully identified from the reference strain, B. thuringiensis subsp. alesti. In addition, the RFLP patterns revealed that B. thuringiensis LY-99 included a novel cry1A-type gene in addition to cry1Aa, cry1Ac, cry1Be and cry1Ea genes. The novel cry1A-type gene was designated cry1Ah2 (Genbank accession No DQ269474). An inverse PCR method was used to amplify the flank regions of cry1Ah2 gene. Finally, 3143 bp HindIII fragment from B. thuringiensis LY-99 plasmid DNA including 5` region and partial ORF was amplified, and sequence analysis revealed that cry1Ah2 gene from LY-99 showed 89.31% of maximum sequence similarity with cry1Ac1 crystal protein gene. In addition, the deduced amino acid sequence of Cry1Ah2 protein shared 87.80% of maximum identity with that of Cry1Ac2. This protein therefore belongs to a new class of B. thuringiensis crystal proteins.

Simple synthesis of Biphosphonates with Excellent Flameproofing Properties

( Hui Zhen Zhi ),( Yang Cao ),( Wei Wei Zhang ),( Xu Feng Yang ),( Jin Fei Yang ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.2

Four excellent halogen-free biphosphonate flame retardants (EBDMP, EBDEP, MBDMP, MBDEP) were synthesized through poly condensation and Arbuzov rearrangement reactions without using any solvents. The products were characterized by ESI-MS, FT-IR and 1H NMR. The application of EBDEP in polyurethane soft foam was studied and the results illustrated its excellent flame-proofing properties.

Polyethersulfone membrane modified by zwitterionic groups for improving anti-fouling and antibacterial properties

Wei Jin,Yu-Fei Lin,Zhen-Liang Xu,Ping-Ping Li,Jia-Yue Dai,Yi-Hao Tong,Xin Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

Ultrafiltration (UF) membranes are commonly confronted with threats from pollutants during long-termoperation. The zwitterions with high hydrophilicity are expected to be the critical material to improve theanti-fouling and antibacterial abilities of membranes. Herein, a new zwitterionic polyethersulfone (PES)(zwitterionic PES - ZPES) was synthesized. The as-prepared ZPES was used to fabricate a zwitterionic UFmembrane via the non-solvent induced phase inversion (NIPS) method. Zwitterionic polymer skeletonenhanced the hydrophilicity, permeability and anti-fouling properties of the material. As a result, theZPES membrane exhibited doubled water flux growth (269.6 Lm2h1) compared with that of the PES(125.3 Lm2h1) and high protein rejection (98.6%). Besides, the ZPES membrane could maintain anexcellent flux recovery rate (94.1%) and antibacterial effect (more than 95%). Therefore, the excellentanti-fouling and antibacterial abilities of the ZPES membrane broaden its application scope further.

SYNTHESIS OF CALCIUM OXALATE ASSEMBLY STRUCTURE AND CONVERSION

ZHI-ZHEN XU,JIN-KU LIU,QING-SHENG WU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2007 NANO Vol.2 No.2

The conversion from one assembly structure to other composite assembly structures is valuable to both theoretical research and actual application in the nano/micromaterials science. In this paper, firstly, the flower-like calcium oxalate assembly structure was synthesized using a supramolecule template; then, through a facile process, the calcium oxalate was converted to a sphere-cluster-like core/shell CaC2O4/CaWO4 nanocomposite assembly structure. The converted product remained the basic structure of original product, and possessed some new optical properties such as fluorescence, etc.

Expanded Waste Ground Rubber Tire Powder/Polypropylene Composites: Processing-Structure Relationships

Xin, Zhen Xiang,Zhang, Zhen Xiu,Zhang, Bao Sheng,Pal, Kaushik,Deng, Xu,Lee, Sung Hyo,Kim, Jin Kuk SAGE Publications 2009 Journal of composite materials Vol.43 No.24

<P>The usage of waste tire powder as dispersed phase in polypropylene matrix offers an interesting opportunity for recycling of the waste tire. In order to obtain ‘value added products’ from polypropylene (PP)/waste ground rubber tire powder (WGRT) composites, in this study, the processing of foamedPP/WGRT composites was investigated using a single-screw foam extrusion setup and chemical blowing agent. The regression models were constructed to study the relationships between the foam structure (i.e., void fraction, average cell size, and cell density) of foamed PP/WGRT composites, the processing conditions (extruder’s die temperature and screw speed), and the formulation compositions (WGRT content and blowing agent concentration) by applying a four-factor central composite design (CCD) statistical approach. The response surface plots generated using the regression models allow the rapid selection of the proper process parameters to obtain PP/WGRT composite foams with the desired density and morphology.</P>

Effects of formulation and processing parameters on the morphology of extruded polypropylene-(waste ground rubber tire powder) foams

Xin, Zhen Xiang,Zhang, Zhen Xiu,Pal, Kaushik,Lu, Bing Xue,Deng, Xu,Lee, Sung Hyo,Kim, Jin Kuk Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of vinyl & additive technology Vol.15 No.4

<P>This paper presents an experimental study of the foaming behavior of polypropylene (PP)/(waste ground rubber tire powder) (WGRT) blends when using a chemical blowing agent in an extrusion foaming process. The effects of formulations (i.e., WGRT content, blowing agent content, compatibilizer) and the processing parameters (i.e., die temperature, screw speed) on the void fraction, average cell size, cell density, and cell morphology of the PP/WGRT foams were investigated. The blowing agent loading affected the cell structure of the foams and the average cell size, and the void fraction increased with increasing blowing agent loading. Both increasing the screw speed and decreasing the die temperature could establish a high pressure drop in the extruder die, and these were beneficial to the foaming extrusion. J. VINYL ADDIT. TECHNOL., 2009. © 2009 Society of Plastics Engineers</P>

Isolation and Characterization of a cDNA Encoding Two Novel Heat-shock Factor OsHSF6 and OsHSF12 in Oryza Sativa L.

Liu, Jin-Ge,Yao, Quan-Hong,Zhang, Zhen,Peng, Ri-He,Xiong, Ai-Sheng,Xu, Fang,Zhu, Hong Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5

As a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and Arabidopsis. In this study, we isolated two putative heat shock factors OsHSF6 and OsHSF12 that interact specifically with heat-shock element (HSE) from Oryza sativa L by yeast one-hybrid method. The full-length cDNA of OsHSF6 and OsHSF12 have 1074bp and 920bp open reading frame (ORF), respectively. Analysis of the deduced amino acid sequences revealed that OsHSF6 was a class A heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor, while OsHSF12 was a class B HSF with C-terminal domain (CTD) lacking of AHA motif. Bioinformatic analysis showed that the sequences and structures of two HSFs' DNA binding domain (DBD) had a high similarity with LpHSF24. The results of RT-PCR indicated OsHSF6 gene was expressed immediately after rice plants exposure to heat stress, and the transcription of OsHSF6 gene accumulated primarily in immature seeds, roots and leaves. However, we did not find the transcription of OsHSF12 gene in different organs and growth periods. Our results implied that OsHSF6 might be function as a HSF regulating early expression of stress genes in response to heat shock, and OsHSF12 might be act as a synergistic factor to regulate the expression of down-stream genes.