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Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation
Huang, Chang-Xin,Zhu, Ying,Duan, Guang-Liang,Yao, Ji-Fen,Li, Zhao-Yang,Li, Da,Wang, Qing-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8
Objective: To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells. Method: After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs. Results: 70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results. Conclusion: Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.
Heating Control Design of a Space Camera
Qing-Lei Zhao,Ke-Hui Long,Feng-Wei Guan,Liang Huang 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.4
When a space camera works on the track, it presents in vacuum environment, under the condition of low temperatures, the camera can only maintain its temperature level by absorption of radiation. If stayed in this environment for a long time, mechanical structure of the space camera may become brittle, lose strength, and an exfoliation phenomenon may appear, in addition, abnormal operation and malfunction of electronic equipments may also occur, these may affect imaging quality of the camera. In this paper, a heating control method is designed for a space flight off-axis camera. Firstly, heating control working platform of the camera is introduced, a design method of heating control is determined. After that, heating control treatment procedure is introduced. Finally, the ground temperature monitor is used to validate correctness of the method designed. The comparison diagram before and after thermal analysis and temperature data obtained from experiments are presented, results show that, the heating control method is simple and effective, it has wide applicability and strong generalization performance.
Qing Huang,Jin-Hua Zhao,Xiu-Hong Liu,Peng Liu,Xue-Lin Wang 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.41
A photonic crystal slab that was constructed as a 2D hexagonal photonic crystal with a finite depth and was etched into an ion-implanted LiNbO_3 waveguide was simulated by using the finitedifference time-domain (FDTD) method. Both the ΓK-oriented and ΓM-oriented propagations were simulated with the fundamental TE mode of the LiNbO_3 waveguide as the incident beam. Despite the weak vertical confinement, the transmittance spectra show that they both have quite deep stop gaps. These gaps show a good overlap. At wavelengths within the overlap region, both the line defects that we created in the ΓK and the ΓM directions worked very well as channel waveguides.
Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats
Huang Ao,Xiao Gefang,Chen Yiliu,Hu Zuying,Lee Pin-Hsuan,Huang Yusen,Zhuang Zifeng,Zhang Yuling,Qing Peng,Zhao Canghuan 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3
Background: In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern. Objectives: In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia. Methods: Pathological tissue from the hypothalamus was analyzed using hematoxylin– eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR. Results: In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (p < 0.05). The concentration of melatonin was significantly increased (p < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (p > 0.05). Conclusion: Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of
Dynamic and Damage Characteristics of Mortar Composite under Impact Load
Qing Li,Tong-de Zhao,Chen Huang,Bing-bing Yu,Zheng-Hua Gao,Kai Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.3
In the process of underground construction, it is common to spray concrete on the working surface, which will form a concrete-rock composite structure, to achieve the expected reinforcement effect. Compared with pure concrete or rock structures, composite structures have different mechanical properties, energy evolution and damage characteristics. Understanding the dynamic mechanics and damage characteristics of composite structures under impact loads is important to the safety of underground engineering construction. First, dynamic compression tests on different grades of mortar-sandstone composite specimens were carried out by using a Hopkinson pressure bar system to discuss the effect of impact velocities on the mechanical properties and failure modes of composite specimens. The results show that the dynamic peak stress, strain energy density and fragmentation block of the mortar-sandstone composite have an obvious strain rate effect. With an increase in impact velocity, the internal cracks of the sample do not have sufficient time to expand, and the energy absorbed by the sample leads to the continuous initiation of new cracks in the sample, resulting in an increasing damage degree of composite specimens. The increase in mortar strength can significantly increase the peak stress, improve the load-bearing capacity of the sample, and reduce the degree of sample damage. At the same impact velocity, the fragmentation degree of the mortar sample is more severe than that of sandstone, and the mortar sample is broken into powder debris, while the sandstone is broken into fragments. In addition, according to the failure properties of the composite sample, the mortar and sandstone samples are described by different damage models, and the dynamic damage constitutive model of the mortar-sandstone composite is established based on the overlay principle. The dynamic test results were fitted. The research shows that the fitting curves of the model are well fitted with the test curves and that the model accurately reflects the changes in the mechanical parameters of the composite specimens.
Electrode properties of porous La2NiO4+δ layers screen-printed on a Ce0.8Sm0.2O2-δ electrolyte
Qing Xu,Bok-Hee Kim,Kai Zhao,Duan-Ping Huang,Min Chen,Hong Wang 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.2
A La2NiO4+δ/Ce0.8Sm0.2O2-δ/La2NiO4+δ symmetrical cell was prepared using a screen-printing method by sintering at 1100℃. The electrochemical properties of the cell were examined by the impedance spectroscopy technique under zero dc bias in air between 500-700℃. The La2NiO4+δ electrodes displayed satisfactory chemical and thermodynamic compatibilities with the Ce0.8Sm0.2O2-δ electrolyte. A comparatively low area specific resistance of 0.18 Ω cm2 at 700℃ was determined for the La2NiO4+δ electrodes. Scanning electron microscope (SEM) observation revealed that the La2NiO4+δ electrodes showed a reasonable porosity, a well-necked connection among fine grains and a tight adhesion to the electrolyte support. The desired microstructure is regarded to be responsible for the superior electrode properties of the La2NiO4+δ layers. A La2NiO4+δ/Ce0.8Sm0.2O2-δ/La2NiO4+δ symmetrical cell was prepared using a screen-printing method by sintering at 1100℃. The electrochemical properties of the cell were examined by the impedance spectroscopy technique under zero dc bias in air between 500-700℃. The La2NiO4+δ electrodes displayed satisfactory chemical and thermodynamic compatibilities with the Ce0.8Sm0.2O2-δ electrolyte. A comparatively low area specific resistance of 0.18 Ω cm2 at 700℃ was determined for the La2NiO4+δ electrodes. Scanning electron microscope (SEM) observation revealed that the La2NiO4+δ electrodes showed a reasonable porosity, a well-necked connection among fine grains and a tight adhesion to the electrolyte support. The desired microstructure is regarded to be responsible for the superior electrode properties of the La2NiO4+δ layers.