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Zhao Wu,Jinlong Zhao,Wenye Sun,Jing Liang,Jing Jiang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.9
Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the con-venient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.
Zhao, Jinlong,Sun, Wenye,Liang, Jing,Jiang, Jing,Wu, Zhao Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.9
Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.
Liang-Yao Chen,Su-Xing Pan,Yun-Fei Wu,Lai Wei,Yu-Xiang Zheng,You-Yuan Zhao,De-Wang Ma 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.4
We have investigated the probing beam transmitance due to the photon-induced anisotropy effect in the bacteriorhodopsin/polymer composite film(bR/PC film) by using the probing-pumping beam method under different laser wavelengths. By using a theoretical simulation, we give the characteristics of the birefringence Δn and the dichroism ΔD for various wavelengths of the probing and the pumping beams.
Study of the Optimal Film Structure by Using Conventional Elipsometry
Liang-Yao Chen,Ming-Yu Sheng,Yun-Hua Wu,Yuan Zhao,Yue-Rui Chen,Yu-Xiang Zheng 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
The optimal film structure affected by the space effect has been studied for SiO2 film samples deposited onto Si wafers by using the e-beam evaporation method. The ellipsometric data were measured at different incidence angles and in the 300-800-nm wavelength range. In the range where the phase delay δ is close to π, the film thickness deduced from the conventional ellipsometry model varied with the phase delay. The optimal SiO2 film-thickness was achieved with a modified ellipsometric model, including the space effect. The results given in this work can generally be applied to other optical measurement methods used to study film structures.
Design and Implementation of Energy Saving Controller for Air-Conditioner in Building
Liang Zhao,Jili Zhang,Yue Shen,Wenyan Wu 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.8
This paper has completed the hardware design of STM32F103 system circuit, infrared code learning module, human body infrared sensor module, indoor temperature and humidity sensor module, completes the software design of learning air conditioning remote control code, indoor temperature and humidity sensor module, also completes the energy-saving controller hardware and software debugging. Finally, this paper takes split type air conditioning units in a conference room as the control object (including indoor lighting system) to complete the energy-saving controller in air conditioner electrical system design, installation and debugging, completes the air conditioning start-stop and infrared temperature control code learning test. The test results show that energy-saving controller has realized infrared automatic perception of human activity, machine start-stop code self-learning and temperature settings and other functions.
Wu, Di,Su, Liang-Cheng,Wu, Xiao-Jie,Zhao, Guo-Dong The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.2
An optimized space vector pulse width modulation (SVPWM) method with common mode voltage elimination and neutral point potential balancing is proposed for an open-end winding induction motor. The motor is fed from both of the ends with two neutral point clamped (NPC) three-level inverters. In order to eliminate the common mode voltage of the motor ends and balance the neutral point potential of the DC link, only zero common mode voltage vectors are used and a balancing control factor is gained from calculation in the strategy. In order to improve the harmonic characteristics of the output voltages and currents, the balancing control factor is regulated properly and the theoretical analysis is provided. Simulation and experimental results show that by adopting the proposed method, the common mode voltage can be completely eliminated, the neutral point potential can be accurately balanced and the harmonic performance for the output voltages and currents can be effectively improved.
Aihui Liang,Gui Huang,Zhiping Wang,Wenjin Wu,Yu Zhong,Shan Zhao 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.5
A novel bis(dibenzo-24-crown-8)-functionalized iridium complex with anemission peak at 665 nm was synthesized. Several deep red-emittingsupramolecualr phosphorescent polymers (SPPs) as a class of solutionprocessableelectroluminescent (EL) emitters were formed by utilizing theefficient non-bonding self-assembly between the resulting iridium complexand bis(dibenzylammonium)-tethered monomers. These SPPs show anintrinsic glass transition with a Tg of ca. 90 °C. The photophysical andelectroluminescent properties are strongly dependent on the hosts’ structuresof the supramolecular phosphorescent polymers. The polymer light-emittingdiode based on SPP3 displayed a maximal external quantum efficiency(EQE) of 2.14% ph·el−1 and the Commission Internationale de L’Eclairage(CIE) coordinates of (0.70, 0.29).
Kun Wu,Yuanbo Xu,Na Yang,Liang Zhao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.3
Fault signals measured from a damaged rolling bearing usually exhibit amplitude modulation (AM)-frequency modulation (FM) properties. For this kind of signal, the demodulation technique is an effective and common method. In the demodulation techniques, a variety of energy operator (EO) tools have been available due to high computational efficiency and self-adaptability to transient signals. Hence, an alternative energy measure is proposed in this work. The new demodulation technique is achieved by combining a multiresolution higherorder symmetric difference sequence with the envelope-derivative operator (EDO). The multiresolution higher-order symmetric envelope-derivative operator (MHOS-EDO) can extract the weak bearing fault characteristics in a harsh environment without any pre-filtering. Especially in the presence of vibration interferences, its unique energy transformation can eliminate the influence of vibration interferences, so that the vibration interferences will no longer appear in the energy spectrum. Through simulation and practical experiments, the superiority of MHOS-EDO in dealing with noise and interfering components is demonstrated. Meanwhile, the comparison results reveal that the MHOS-EDO outperforms some previous EO and some other bearing fault identification methods.
Di Wu,Liang-Cheng Su,Xiao-Jie Wu,Guo-Dong Zhao 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.2
An optimized space vector pulse width modulation (SVPWM) method with common mode voltage elimination and neutral point potential balancing is proposed for an open-end winding induction motor. The motor is fed from both of the ends with two neutral point clamped (NPC) three-level inverters. In order to eliminate the common mode voltage of the motor ends and balance the neutral point potential of the DC link, only zero common mode voltage vectors are used and a balancing control factor is gained from calculation in the strategy. In order to improve the harmonic characteristics of the output voltages and currents, the balancing control factor is regulated properly and the theoretical analysis is provided. Simulation and experimental results show that by adopting the proposed method, the common mode voltage can be completely eliminated, the neutral point potential can be accurately balanced and the harmonic performance for the output voltages and currents can be effectively improved.