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RISS 인기검색어
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- 저자 : Zhang Yujuan (검색결과 5 건)
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( Qiang Zhang ),( Yujuan Li ),( Fangxue Xu ),( Mengmeng Zheng ),( Xiaozhi Xi ),( Xuelan Zhang ),( Chunchao Han ) 한국균학회 2017 Mycobiology Vol.45 No.2
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NH<sub>4</sub>NO<sub>3</sub> was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at 20.67 μg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NH<sub>4</sub>NO<sub>3</sub> was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 μg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.
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Gong Huihui,You Jun,Zhang Xiurong,Liu Yanzhong,Zhao Fengtao,Cui Xinxiao,Zhang Yujuan 한국식물학회 2021 Journal of Plant Biology Vol.64 No.6
Long non-coding RNAs (lncRNAs) play important roles in various biological regulatory processes in which complicated mechanisms are involved, as well as stress-responsive regulation. However, the number, characteristics, sequences and possible effects of lncRNAs in sesame response to salt stress are poorly understood. In this study, a total of 2482 lncRNAs were identified from two contrasting sesame genotypes under salt stress using high-throughput RNA sequencing, of which 599 were intergenic lncRNAs, 293 were antisense lncRNAs and 786 lncRNAs may encode proteins. Expression pattern analysis showed that most lncRNAs were expressed at a low level and a total of 700 differentially expressed lncRNAs were characterized as salt responsive in sesame. A large number of potential target genes of lncRNAs were predicted, and functional annotation analysis indicated that the differentially expressed lncRNAs in salt stress may regulate protein-coding genes involved in several important pathways related to glycolysis/gluconeogenesis, flavonoid biosynthesis, monoterpenoid biosynthesis, biotin metabolism, galactose metabolism, cyanoamino acid metabolism and carotenoid biosynthesis. Integrated analysis of lncRNAs and mRNAs revealed the regulatory role of lncRNAs associated with salt resistance in sesame, and provided convincing proof of the interplay of specific candidate target genes with lncRNAs. Our results indicated that a comprehensive set of lncRNAs with potential target genes were responsive to salt stress in sesame seedlings. These findings provided important information on salinity responses and adaptation of sesame to salt stress and may constitute useful resources for more comprehensive studies on gene regulation in sesame.
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Wang Zhenjie,Wang Yujuan,He Yuan,Zhang Ning,Chang Wei,Niu Yahui 한국통합생물학회 2020 Animal cells and systems Vol.24 No.5
Gastric cancer, one of the most common malignant tumors of the digestive tract, is devoid of effective treatment owing to its highly invasive ability. Aquaporins (AQPs), transmembrane water channel proteins, has been shown to be involved in the malignancy of gastric cancer. This study aims to investigate the pathophysiological roles of AQP-1 in gastric cancer. We first demonstrated quantitative real-time polymerase chain reaction analysis and found upregulation of AQP-1 in gastric cancer cell lines. Additionally, silence of AQP-1 inhibited cell proliferation via decrease of proliferating cell nuclear antigen (PCNA) and minichromosome maintenance complex component 2 (MCM2). Moreover, migration and invasion of gastric cancer cells were also suppressed by the interference of AQP-1. However, the tumorigenic mechanism of AQP-1 on gastric cancer is yet to be found. We demonstrated western blot analysis and found that knockdown of AQP-1 decreased protein expression of phospho (p)-GRB7 (growth factor receptor-bound protein 7) and led to a remarkable reduction of p-extracellular signalregulated kinase (ERK) via inactivation of RAS. In general, our findings indicated that AQP-1 facilitates proliferation and invasion of gastric cancer cells via GRB7-mediated ERK and Ras activation, illuminating a novel AQP-1-RAS/ERK molecular axis as regulator in gastric cancer progression and suggesting potential implications in the treatment of gastric cancer.
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WenJuan Yan,YingYing Cai,Qun Zhang,YuSi Liu,WenChun Xu,YiBing Yin,YuJuan He,Hong Wang,XueMei Zhang 한국미생물학회 2013 The journal of microbiology Vol.51 No.4
Hsp100/Clp proteins have crucial functions in the protein quality control, stress tolerance, and virulence of many pathogenic bacteria. ClpE is an important virulence factor involved in adherence and invasion in Streptococcus pneumoniae. To explore the underlying mechanism, we screened ClpE interaction proteins using a bacterial two-hybrid system and co-immunoprecipitation. We used ClpE as bait and constructed the pBT-ClpE bait plasmid for two-hybrid screening. Then, we constructed ClpE::GFP fusion for co-immunoprecipitation screening using anti-GFP monoclonal antibody. We obtained eight potential ClpE interaction proteins,including carbamoyl-phosphate synthase, pyruvate oxidase (SpxB), phosphoenolpyruvate-protein phosphotransferase,aminopeptidase N (pepN), L-lactate dehydrogenase, ribosomal protein S4, sensor histidine kinase (SPD_2019), and FtsW (a cell division protein). FtsW, SpxB, pepN, and SPD_2019 were confirmed to interact with ClpE using Bacterial Two-hybrid or Co-immunoprecipitation. Morphologic observations found that ΔclpE strain existed in abnormal division. β-Galactosidase activity assay suggested that ClpE contributed to the degradation of FtsW. Furthermore, FtsW could be induced by heat shock. The results suggested that ClpE might affect cell division by regulating the level of FtsW. These data may provide new insights in studying the role of ClpE in S. pneumoniae.
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