20(S)-Ginsenoside Rg3-induced apoptosis in HT-29 colon cancer cells is associated with AMPK signaling pathway.

Yuan, Hai-Dan,Quan, Hai-Yan,Zhang, Ya,Kim, Sung Hoon,Chung, Sung-Hyun D. A. Spandidos 2010 MOLECULAR MEDICINE REPORTS Vol.3 No.5

<P>20(S)-ginsenoside Rg3 [20(S)-Rg3)], one of the main constituents isolated from Panax ginseng, has been shown to have an anti-cancer effect and to induce apoptosis by interfering with several signaling pathways. However, the molecular mechanisms of AMP-activated protein kinase (AMPK) associated with apoptosis in HT-29 colon cancer cells remain unclear. In the present study, we investigated whether 20(S)-Rg3 exerts an anti-proliferative effect and induces apoptosis by modulating the AMPK signaling pathway in HT-29 cells. 20(S)-Rg3-treated cells displayed several apoptotic features, including DNA fragmentation, proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) and morphological changes. 20(S)-Rg3 down-regulated the expression of anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl2), up-regulated the expression of pro-apoptotic protein of p53 and Bcl-2-associated X protein (Bax), and caused the release of mitochondrial cytochrome c, PARP, caspase-9 and caspase-3. However, 20(S)-Rg3-induced apoptosis was completely abolished in the presence of compound C (AMPK inhibitor) or small interfering RNA for AMPK (siAMPK). In addition, STO-609 (CaMKKβ inhibitor) attenuated 20(S)-Rg3-induced AMPK activation and apoptosis. These results suggest that 20(S)-Rg3-induced apoptosis in HT-29 cells is mediated via the AMPK signaling pathway, and that 20(S)-Rg3 is capable of inducing apoptosis in colon cancer.</P>

MiR-193b enhanced proliferation and migration and inhibits apoptosis through targeting RAB7A in osteosarcoma cell

Zhang Yuan-yuan,Xu Hai-yan,Dai Jing-jing 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.1

Background Accumulating study indicated that microRNA (miRNA) played critical role in the osteosarcoma (OS). The role and mechanisms of miR-193b in OS cell lines were still unknown. Objective We resolved the miR-193b expression in OS cell line and normal cell by RT-PCR assay. The effects of upregulated miR-193b on OS cell proliferation, migration, invasion and apoptosis were evaluated using CCK-8 assay, transwell assay, would-healing assay and flow cytometric analysis in vitro, respectively. We investigated the effect of upregulated miR-193b on the mRNA level of cell cycle protein CCND1 and CCNE1 using RT-PCR assay and the protein level of epithelial to mesenchymal transition (EMT)-related protein E-cadherin, vimentin, and N-cadherin by western blotting assay in MG-63 and U2SO cells. Furthermore, luciferase reporter assays were employed to identify the candidate target gene RAB7A of miR-193b. Results The expression of miR-193b was downregulated in OS cells. In MG-63 and U2SO cells, ectopic miR-193b expression inhibited cell proliferation, migration, invasion, and induced apoptosis. We found that miR-193b reduced the mRNA expression of CCND1 and CCNE1, and regulated the associated proteins of EMT including E-cadherin, vimentin and N-cadherin in MG-63and U2SO cell lines. Moreover, the candidate target gene RAB7A was negatively regulated by miR-193b. In addition, upregulated RAB7A rescued the inhibitory effect of miR-193b mimics on the development of OS cell. Conclusion In conclusion, this study suggested that miR-193b overexpression inhibited cell proliferation, migration, invasion, and induced cell apoptosis by down-regulating RAB7A in OS cell lines.

A ppb-Level Formaldehyde Gas Sensor Based on Rose-Like Nickel Oxide Nanoparticles Prepared Using Electrodeposition Process

Yong Zhang,Long-Zhen Xie,Chao-Xin Yuan,Chun-Lin Zhang,Su Liu,Ying-Quan Peng,Hai-Rong Li,Miao Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.1

In this study, rose-like nickel oxide nanoparticles (diameter of 400–500 nm) were prepared on indium tin oxide (ITO) glass substrates by a simple electrodeposition in NiSO4·6H2O solution. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and transmission electron microscope (TEM) were used for analysis of the NiO nanoparticles. The effects of operating temperature on the sensor response and the response versus gas concentration properties of the NiO nanorose-based sensors were investigated. We determined the operating temperature of the gas sensors to be 230℃, considering the proper sensitivity and a rapid response. In addition, gas-sensing characteristics of rose-like NiO nanoparticles to formaldehyde were investigated. It was shown that the sensors exhibited good response (Rg/Ra = 3.43) properties to formaldehyde gas at 230℃, making them to be promising candidates for practical detectors to formaldehyde gas.

고지방 식이로 유도된 비만 쥐에서 HPJ 추출물의 항비만 효과

원해단(Hai-Dan Yuan),임방호(Bang-Ho Lim),김성집(Sung-Jib Kim),권해연(Hai-Yan Quan),장아(Ya Zhang),신대희(Dae-Hee Shin),정성현(Sung-Hyun Chung) 대한약학회 2009 약학회지 Vol.53 No.5

In this study, we investigated the anti-obese activity of HPJ extract in C57BL/6J mice. The C57BL/6J mice were randomly divided into five groups: normal control group (Con), high fat diet control group (HFD), treatment groups with HPJ at 125 mg/kg (HPJ125), 250 mg/kg (HPJ250), or 500 mg/kg (HPJ500). To induce an obesity, mice were fed by a high fat diet for 6 weeks, and mice were administered with HPJ extract once a day for 8 weeks. At the end of treatment, we examined the effect of HPJ extract on body weight, plasma lipid, and lipogenic enzymes. HPJ extract was found to lower whole body and epididymal adipose tissue weights and lowered plasma levels of glucose, insulin, triglyceride (TG), total cholesterol (TC), non-esterified fatty acid (NEFA) and leptin, compared to those in HFD group. Histological analyses of the liver and fat tissues of mice treated with HPJ extract revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the HFD group. In addition, HPJ extract preserved the morphological integrity of pancreatic islets. To elucidate an action mechanism of HPJ extract, Western blot and RT-PCR were performed using epididymal adipose tissues. HPJ extract up-regulated the levels of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and its substrate, acetyl-CoA carboxylasse (ACC). HPJ extract also attenuated lipogenic gene expressions of sterol regulatory element-binding protein 1α (SREBP1α), fatty acid synthase (FAS), sterol-CoA desaturase 1 (SCD1) and glycerol-3-phosphate acyltransferase (GPAT) in dose-dependent manners. In contrast, expressions of lipolytic genes such as peroxisome proliferator-activated receptor-α (PPAR-α) and CD36, and fatty acid β-oxidation gene, carnitine palmitoyltransferase-1 (CPT-1) were increased. These results suggest that HPJ extract ameliorates obesity through inhibiting synthesis of lipogenic enzymes as well as stimulating fatty acid oxidation resulting from activation of AMPK, and HPJ extract could be developed as a potential therapeutic agent for obese patients.

Immortalization of Swine Umbilical Vein Endothelial Cells with Human Telomerase Reverse Transcriptase

Hai Xia Hong,Yan Ming Zhang,Hao Xu,Zheng Yuan Su,Pei Sun 한국분자세포생물학회 2007 Molecules and cells Vol.24 No.3

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

Zhang, Hai,Huang, Chang-Jun,Tian, Yuan,Wang, Yu-Ping,Han, Ze-Guang,Li, Xiang-Cheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Family with sequence similarity 189, member B (FAM189B), alias COTE1, a putative oncogene selected by microarray, for the first time was here found to be significantly up-regulated in hepatocellular carcinoma (HCC) specimens and HCC cell lines. mRNA expression of COTE1 in HCC samples and cell lines was detected by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR, while protein expression of COTE1 in HCC tissues was assessed by immunohistochemistry. In addition, invasion of HCC cells was observed after overexpressing or silencing COTE1. In the total of 48 paired HCC specimens, compared with the adjacent non-cancer tissues, the expression of COTE1 was up-regulated in 31 (p<0.01). In HCC cell lines, COTE1 expression was significantly higher than in normal human adult liver (p<0.01). Overexpression of COTE1 enhanced HCC-derived LM6 and MHCC-L cellular invasion in vitro. In contrast, COTE1 knockdown via RNAi markedly suppressed these phenotypes, as documented in LM3 and MHCC-H HCC cells. Mechanistic analyses indicated that COTE1 could physically associate with WW domain oxidoreductase (WWOX), a tumor suppressor. COTE1 may be closely correlated with invasion of hepatocellular carcinoma (HCC) cells and thus may serve as an effective target for gene therapy.

Impact of Adjuvant Chemotherapy Cycles on Prognosis of Resectable Stomach Cancer: A Retrospective Analysis

Zhang, Wen-Ying,Zhang, Wen-Jun,Bai, Yu,Yuan, Hai-Hua,Liu, Feng,Gao, Jun,Gong, Yan-Fang,Jiang, Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Aims: The aim of this study was to investigate the effects of adjuvant chemotherapy cycles on the prognosis of patients with post-operative stomach cancer through retrospective analysis. Methods: A total of 128 patients with gastric cancer who underwent gastrectomy, followed by adjuvant chemotherapy consisting of epirubicin, cisplatin or oxaliplatin, leucovorin, and 5-fluorouracil, according to a defined schedule, were divided into three groups according to the number of chemotherapy cycles: Group I (<6 cycles); Group II (6 cycles); and Group III (>6 cycles). Results: The 5-year overall survival (OS) was 20.8% in Group I, 45.0% in Group II, and 42.9% in Group III, with a median follow-up of 43 months. The 5-year relapse-free survival (RFS) was 15.1% in Group I, 40% in Group II, and 40% in Group III. The OS and RFS in Groups II and III were significantly better than in Group I (OS, p = 0.002 and p=0.003; RFS, P<0.001 and P=0.002). There was no difference in OS (p = 0.970) or in RFS (p = 0.722) between Groups II and III. Multivariate Cox hazard analysis determined that the number of adjuvant chemotherapy cycles was an independent factor that influenced OS and RFS. Conclusion: Six cycles of adjuvant chemotherapy gave encouraging outcomes in patients with resectable gastric cancer. Further prospective randomized controlled investigations are warranted in a multi-center setting.

Korean Red Ginseng Attenuates Hepatic Lipid Accumulation via AMPK Activation in Human Hepatoma Cells

Hai-Yan Quan,Hai-Dan Yuan,Do Yeon Kim,Ya Zhang,정성현 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.1

In this study, we examined Korean red ginseng (KRG) extract affects on the lipid metabolism in HepG2cells. Increase in AMP-activated protein kinase (AMPK)phosphorylation was observed when the cells were treated with KRG. Activation of AMPK was also demonstrated by measuring the phosphorylation of acetyl-CoA caboxylase (ACC), a substrate of AMPK. KRG down-regulated gene expressions of sterol regulatory element binding protein 1c (SREBP1c) and its target proteins, such as fatty acid synthase (FAS) and stearoyl-CoA desaturase (SCD1) in time- and dose-dependent fashions. In contrast, gene expressions of peroxisome proliferator-activated receptor α(PPARα) and CD36 were increased. These effects were reversed in the presence of compound C, an AMPK inhibitor. However, there were no differences in gene expressions of SREBP2, 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, and low-density-lipoprotein receptor (LDLR). Taken together, KRG induced supression of SREBP1c and activation of PPARα via AMPK and these effects seem to be one of anti-hyperlipidemic mechanism of KRG in HepG2 cells.

Generator Bearing Fault Diagnosis Based on Analytic Hierarchy Process and Binary-tree Support Vector Machine

ZHANG Xing-yuan,CHEN Minye,XU Hai-rong,LI Pei-qi 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.8

Binary-tree support vector machine (SVM) has such advantages as small repeated operation workload, fast classification speed and dead zone inexistence, but the structural design can influence the classification accuracy thereof. In order to rationally design the structure of the binary-tress SVM, a multi-classification algorithm (AHP-BSVM) combining analytic hierarchy process (AHP) and binary-tree SVM is proposed in this paper. Firstly, the analytic hierarchy process is adopted to establish the evaluation system model so as to comprehensively evaluate multiple influencing factors for determining the weight values of various faults; then, the faults are ordered by the weight values and the structure of the binary-tree SVM is determined according to the fault sequence; finally, the proposed algorithm is adopted for fault diagnosis and analysis. The simulation experiment shows: compared with other algorithms, the proposed algorithm has higher recognition accuracy and higher classification accuracy, and is applicable to multi-classification, thus having good promotion prospect.

Pan-cancer Analysis of Tumor Mutational Burden and Homologous Recombination DNA Damage Repair Using Targeted Next-Generation Sequencing

Hai-Yun Wang,Ling Deng,Ying-Qing Li,Xiao Zhang,Ya-Kang Long,Xu Zhang,Yan-Fen Feng,Yuan He,Tao Tang,Xin-Hua Yang,Fang Wang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Current variability in methods for tumor mutational burden (TMB) estimation and reporting demonstrates the urgent need for a homogeneous TMB assessment approach. Here, we compared TMB distributions in different cancer types using two customized targeted panels commonly used in clinical practice. Materials and Methods TMB spectra of 295- and 1021-gene panels in multiple cancer types were compared using targeted next-generation sequencing (NGS). The TMB distributions across a diverse cohort of 2,332 cancer cases were then investigated for their associations with clinical features. Treatment response data were collected for 222 patients who received immune-checkpoint inhibitors (ICIs) and their homologous recombination DNA damage repair (HR-DDR) and programmed death-ligand 1 (PD-L1) expression were additionally assessed and compared with the TMB and response rate. Results The median TMB between gene panels was similar despite a wide range in TMB values. The highest TMB was eight and 10 in patients with squamous cell carcinoma and esophageal carcinoma according to the classification of histopathology and cancer types, respectively. Twenty-three out of 103 patients (22.3%) were HR-DDR–positive and could benefit from ICI therapy; out of those 23 patients, seven patients had high TMB (p=0.004). Additionally, PD-L1 expression was not associated with TMB or treatment response among patients receiving ICIs. Conclusion Targeted NGS assays demonstrated the ability to evaluate TMB in pan-cancer samples as a tool to predict response to ICIs. In addition, TMB integrated with HR-DDR–positive status could be a significant biomarker for predicting ICI response in patients.