A transcription factor TaTCP20 regulates the expression of Ppd-D1b in common wheat

Wei Fan,Song Tianqi,Zhou Jianfei,Cheng Jie,Li Ruibo,Yu Ming,Zhang Yunrui,Yu-Yang Song,Zhang Bo,Zhang Xiaoke 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Photoperiod (Ppd) genes play an important role in the adaptation of wheat to the ecological environment. However, the transcriptional regulation mechanism of photoperiodic genes has remained elusive. This study isolated a full-length promoter of Ppd-D1b (2518 bp) from the common wheat genome. Several essential core cis-acting elements and numerous light-responsive cis-acting regulatory elements were identifed in Ppd-D1b promoter by the in-silico analysis. Ten 5’-deleted length fragments of the Ppd-D1b promoter fused with GUS were constructed and named D0 ~D9, then transferred them into Arabidopsis thaliana. GUS gene driven by full-length (D0) in transgenic Arabidopsis thaliana showed the same rhythm with Ppd-D1b in wheat under short-day conditions (SDs, 8-h light/16-h dark). The expression of GUS gene in D0 reached its peak at 3 h after dawn, then decreased to the lowest and remained stable. Analysis of the series of 5’-deleted fragments showed that at 3 h after dawn, GUS gene expression activity decreased signifcantly in D7a due to removal of CHEBS (CCA1 HIKING EXPEDITION binding site). Moreover, yeast one-hybrid (Y1H) and dual-luciferase (dual-LUC) assays revealed that TaTCP20 could bind to the Ppd-D1b promoter to increase its transcriptional activity. This study revealed a transcription factor, TaTCP20, which activated Ppd-D1b by binding to CHEBS, provided a foundation for the theoretical research on wheat’s photoperiodic response mechanism.

Enhanced Online Strip Crown Prediction Model Based on KCGAN-ELM for Imbalanced Dataset

Xiaoke Hu,Xiaomin Zhou,Hongfei Liu,Hechuan Song,Shuaikun Wang,Hongjia Zhang 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.8

The strip crown directly affects the quality of strip steel. To enhance online crown control and product quality, an accurate and efficient strip crown prediction model is crucial. The accuracy of the prediction model is determined by the algorithm and dataset used in the modeling process. The accuracy can be enhanced by increasing algorithm complexity but it does not meet the requirements of online applications. Besides, the datasets collected for strip crown prediction are usually imbalanced, which impacts the modeling accuracy. In this paper, an enhanced strip crown prediction model based on KCGAN-ELM is established. A new hybrid algorithm KCGAN is proposed to deal with the imbalanced datasets. ELM is used to establish the strip crown prediction model. To meet the requirements of online applications, incremental learning is introduced, enabling the prediction model to update in real-time based on new production data. On this basis, an update strategy is devised to ensure the prediction model can maintain qualified prediction ability during the updating process. The experiment results indicate that the model trained with the dataset processed by KCGAN demonstrates a significantly enhanced prediction accuracy, achieving an RMSE of 2.86 μm and an R2 value of 0.97. The proposed update strategy enhances the stability of prediction capability during the model updating process.

Development of SSR molecular markers for Allium mongolicum

Jing Hu,Xiaoke Hu,Qian Zhang,Jinhu Zhang,Baoli Fan,Qiushi Yu 한국유전학회 2017 Genes & Genomics Vol.39 No.12

Allium mongolicum is high palatability, nutrient, medicinal value and drought resistance wild plant. However, a lack of molecular markers of this plant results in a series of genetic questions remain largely unknown, including molecular marker data, population genetic structure and accuracy of genetic breeding. In this study, a total of 1,409,706 quality- filtered and trimmed reads were obtained from the normalized genomic DNA of pooled A. mongolicum individuals. These sequences were assembled into 2,093,593 high quality contigs, and a total of 219 simple sequence repeats loci were screened. Sixty of them were selected to validate amplification and to determine the degree of polymorphism in the genomic DNA pools. Fifteen primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism. The number of alleles in ten geographically diverse A. mongolicum populations ranged from 3 to 8 per locus. The observed and expected heterozygosities ranged from 0.350 to 0.860 and 0.516 to 0.931, respectively. Our results provide a valuable resource for A. mongolicum research.

Performance improvement of amorphous indium-gallium-zinc oxide ReRAM with SiO2 inserting layer

Yanli Pei,Biaoren Mai,Xiaoke Zhang,Ruiqin Hu,Ya Li,Zimin Chen,Bingfeng Fan,Jun Liang,Gang Wang 한국물리학회 2015 Current Applied Physics Vol.15 No.4

In this study, the resistive switching performance of amorphous indiumegalliumezinc oxide (a-IGZO) resistive switching random-access memory (ReRAM) was improved by inserting a thin silicon oxide layer between silver (Ag) top electrode and a-IGZO resistive switching layer. Compared with the single a-IGZO layer structure, the SiO2/a-IGZO bi-layer structure exhibits the higher On/Off resistance ratio larger than 103, and the lower operation power using a smaller SET compliance current. In addition, good endurance and excellent retention characteristics were achieved. Furthermore, multilevel resistance states are obtained through adjusting SET compliance current and RESET stop voltage, which shows a promise for high-performance nonvolatile multilevel memory application.

Identification of a 193 bp promoter region of TaNRX1‑D gene from common wheat that contributes to osmotic or ABA stress inducibility in transgenic Arabidopsis

Cheng Jie,Wei Fan,Zhang Mingfei,Li Nan,Song Tianqi,Wang Yong,Chen Dongsheng,Xiang Jishan,Zhang Xiaoke 한국유전학회 2021 Genes & Genomics Vol.43 No.9

Background Cloning and characterizing the drought-inducible promoters is essential for their use in crop resistance’s genetic improvement. Previous studies have shown that the TaNRX1-D gene participates in regulating the response of wheat to drought stress. However, its promoter has not yet been identifed. Objective In this study, we aimed to characterize the promoter of the TaNRX1-D gene. Methods The promoter of TaNRX1-D (named P0, 2081 bp) was isolated from common wheat with several cis-acting elements that regulate in response to abiotic stresses and some core cis-acting elements. Functional verifcation of the promoter, eight 5′-deletion fragments of TaNRX1-D promoter, was fused to the β-glucuronidase (GUS) gene P0::GUS~P7::GUS and transformed into Arabidopsis, respectively. Agrobacterium-mediated GUS transient assay the P6a and P6b promoter regions in tobacco leaves under normal, osmotic or ABA stress. Results Activity analysis of the full-length promoter (P0) showed that the intensity of stronger β-glucuronidase (GUS) staining in the roots and leaves was obtained during the growth of transgenic Arabidopsis. P0::GUS displayed the GUS activity was much higher in the roots and leaves than in other parts of the transgenic plant under normal conditions, which was similarly within wheat. Analysis of the 5′-deletion fragments revealed that P0::GUS~P6::GUS responded well upon exposure to osmotic (polyethylene glycol-6000, PEG6000) and abscisic acid (ABA) stress treatments and expressed signifcantly higher GUS activity than the CaMV35S promoter (35S::GUS), while P7::GUS did not. GUS transient assay in tobacco leaves showed that the GUS activities of P6a and P6b were lower than P6 in the PEG6000 and ABA stresses. Conclusion The 193 bp (P6) segment was considered the core region of TaNRX1-D responding to PEG6000 or ABA treatment. GUS activity assay in transgenic Arabidopsis showed that this segment was sufcient for the PEG6000 or ABA stress response. The identifed 193 bp promoter of TaNRX1-D in this study will help breed osmotic or ABA tolerant crops. The 36 bp segment between P6 and P6b (−193 to −157 bp) was considered the critical sequence for the TaNRX1-D gene responding to PEG6000 or ABA treatment.

A high-resolution timescale for the Miocene Shanwang diatomaceous shale lagerstätte (China): development of Wavelet Scale Series Analysis for cyclostratigraphy

Jifeng Yu,Xinlong Pang,Wenzhao Fu,Jason Hilton,Mingmei Liang,Zongkai Jiang,Xiuli Zhao,Wenyan Qiao,Suo Shi,Diandong Zhang,Huitao Cao,Haibo Jia,Yadong Wang,Xiaoke Hu,Rui Zhang 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.5

The Miocene aged Shanwang Formation from the Shanwang National Geopark in China represents a succession of lacustrine diatomaceous shales containing an abundant and diverse biota with lagerstätte fossilization of soft tissues. To date, the Shanwang Formation has not been investigated for cyclostratigraphy nor has it been dated with high precision methods. Now we use thorium data as a paleoenvironmental and paleoclimatic proxy to conduct a detailed cyclostratigraphic analysis. A new and simple cyclostratigraphic method, Wavelet Scale Series Analysis (WSSA) is developed to recognize Milankovitch cycles. A total of three short eccentricity and fifteen precession cycles are identified; obliquity cycles are not apparent. In the sedimentary succession, the corresponding precession and short eccentricity cycles are 1.17 m and 4.98 m thick respectively, with this verified by Correlation Coefficient (COCO) analysis and Multitaper-Method (MTM) spectral analysis. We estimate the studied interval was deposited over a duration of 0.3 Myr with a depositional rate of c. 5.7 cm/kyr. Paleomagnetic and radio isotope dating data shows that the diatomaceous shale was deposited during Chron C5En, which places it at approximately 18.5 Ma during the Burdigalian stage of the Early Miocene, rather than in the Middle Miocene as previously thought. The Shanwang lagerstätte biota therefore predates the Middle Miocene Climate Optimum (MMCO) and did not form within it. The geological time scale with a high resolution of 20 kyr was set accordingly.