Effects of Addition Level and Chemical Type of Propionate Precursors in Dicarboxylic Acid Pathway on Fermentation Characteristics and Methane Production by Rumen Microbes In vitro

Li, X.Z.,Yan, C.G.,Choi, S.H.,Long, R.J.,Jin, G.L.,Song, Man K. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.1

Two in vitro experiments were conducted to examine the effects of propionate precursors in the dicarboxylic acid pathway on ruminal fermentatation characteristics, $CH_4$ production and degradation of feed by rumen microbes. Fumarate or malate as sodium salts (Exp. 1) or acid type (Exp. 2) were added to the culture solution (150 ml, 50% strained rumen fluid and 50% artificial saliva) to achieve final concentrations of 0, 8, 16 and 24 mM, and incubated anaerobically for 0, 1, 3, 6, 9 and 12 h at $39^{\circ}C$. For both experiments, two grams of feed consisting of 70% concentrate and 30% ground alfalfa (DM basis) were prepared in a nylon bag, and were placed in a bottle containing the culture solution. Addition of fumarate or malate in both sodium salt and acid form increased (p<0.0001) pH of culture solution at 3, 6, 9 and 12 h incubations. The pH (p<0.0001) and total volatile fatty acids (VFA, p<0.05) were enhanced by these precursors as sodium salt at 3, 6 and 9 h incubations, and pH (p<0.001) and total VFA (p<0.01) from fumarate or malate in acid form were enhanced at a late stage of fermentation (9 h and 12 h) as the addition level increased. pH was higher (p<0.001) for fumarate than for malate as sodium salt at 3 h and 6 h incubations. Propionate ($C_3$) proportion was increased (p<0.0001) but those of $C_2$ (p<0.05) and $C_4$ (p<0.01 - p<0.001) were reduced by the addition of sodium salt precursors from 3 h to 12 incubation times while both precursors in acid form enhanced (p<0.011 - p<0.0001) proportion of $C_3$ from 6h but reduced (p<0.018 - p<0.0005) $C_4$ proportion at incubation times of 1, 3, 9 and 12 h. Proportion of $C_3$ was increased (p<0.05 - p<0.0001) at all incubation times by both precursors as sodium salt while that of $C_3$ was increased (p<0.001) from 6h but $C_4$ proportion was decreased by both precursors in acid form as the addition level increased. Proportion of $C_3$ was higher (p<0.01 - p<0.001) for fumarate than malate as sodium salt from 6 h incubation but was higher for malate than fumarate in acid form at 9 h (p<0.05) and 12 h (p<0.01) incubation times. Increased levels (16 and 24 mM) of fumarate or malate as sodium salt (p<0.017) and both precursors in acid form (p<0.028) increased the total gas production, but no differences were found between precursors in both chemical types. Propionate precursors in both chemical types clearly reduced (p<0.0001 - p<0.0002) $CH_4$ production, and the reduction (p<0.001 - p<0.0001) was dose dependent as the addition level of precursors increased. The $CH_4$ generated was smaller (p<0.01 - p<0.0001) for fumarate than for malate in both chemical types. Addition of fumarate or malate as sodium type reduced (p<0.004) dry matter degradation while both precursors in both chemical types slightly increased neutral detergent fiber degradability of feed in the nylon bag.

홀스타인 수소에 있어서 Chromium Picolinate 의 첨가가 성장성적, 혈액성상 및 도체품질에 미치는 영향

홍중산,김명국,김윤학,한승은,이홍구,이현준,최윤재 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.4

본 시험은 성장단계별로 서로 다른 수준의 CrP를 첨가하여 성장성적, 혈액성상 및 도체품질에 미치는 영향을 규명하고자 실시하였다. 시험 Ⅰ에서 일당증체, 일당건물섭취량 및 사료효율에는 유의적인 차이가 없었다(p<0.05). 혈액성상에서 혈장 Glucose와 PUN의 농도에는 영향이 없었지만 혈중 인슐린의 농도는 0.05% CrP첨가구가 대조구의 2배되는 1.47ng/㎖ 인데 반하여 혈중 NEFA의 농도는 59.00 mEq/㎗ 로 유의적으로 낮게 나타났다(P<0.05). 이는 인슐린이 농도의 증가 및 인슐린의 활성의 증가로 인하여 지방합성대사가 활발히 진행된 결과로 사료된다. 이러한 결과를 도체분석결과가 뒷받침 해주고 있는데 비록 통계적인 유의차는 없지만 0.05% CrP 첨가구에서는 첫 60일간, 0.025% CrP 첨가구에서는 첫 120일간 대조구보다 우수한 성적을 나타냈다. 혈장 Glucose와 PUN의 농도에는 영향이 없었다. 통계적인 유의차는 없었지만 혈 중 인슐린의 농도는 0.025% CrP 첨가구는 대조구보다 8.88% 높은 1.35ng/㎖로 나타났고 0.05% CrP 첨가구는 오히려 1.13ng/㎖로 대조구보다 낮게 나타났다. NEFA의 농도는 시험 Ⅰ에서와 같은 차이는 없었지만 CrP 첨가수준이 증가할수록 감소하는 추세를 나타냈고 등지방두께는 일정한 변화추세를 보이지 않았다. We conducted two experiments to evaluate the effects of chromium picolinate(CrP) on growth performance, carcass characteristics and plasma components in Holstein bulls. In trial Ⅰ, eight finishing Holstein bulls(300±6.99㎏) were allocated to 2 treatments(control and 0.05% CrP) with 4 replication for 10-months. In results, growth performance was not affected by CrP addition. The plasma insulin concentration in 0.05% CrP group was about 2 times higher than the control group of Holstein bulls. The levels of plasma NEFA were significantly decreased to 59.00 mEq/㎗ with 0.05% CrP treatment(P<0.05), but the levels of plasma glucose and PUN were not altered by 0.05% CrP treatment. The grade of carcass was not different between control and 0.05% CrP group, but back fat thickness in 0.05%, CrP group was increased in 22.33% compared with control group. In trial 2, fifteen growing-finishing Holstein bulls(160±4.63㎏) were allocated to 3 treatments(control, 0.025% CrP and 0.05% CrP) with 5 replication for 14-months. During the overall experimental period, growth performance was not affected by CrP levels. The levels of hormone and metabolites were not affected by CrP supplementation. The carcass characteristics were not different between control and treatment. These results show that the CrP may have no effects for beef cattle production because of degradation of CrP conjugation in the rumen. However treatment of short term provide a possibility the effects of development for lipogenesis.

양질 조사료의 보충과 황토의 첨가수준이 한우의 성장성적, 육질 등급 및 경제성에 미치는 영향

홍중산,이홍구,이보균,김준식,최윤재,김윤학,김명국 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.1

본 연구는 6개월령의 한우 36두를 이용하여 양질 조사료의 보충(rice straw;RT, rice straw+cubed roughage;RCT)과 황토의 첨가수준(0%, 1%, 2% 황토)이 생산성, 육질 및 경제적 효과에 미치는 영향을 규명하기 위하여 실시하였다. 실험기간동안 볏짚에 양질 조사료의 보충 (cubed roughage, RCT)은 볏짚단독 처리(RT)에 비하여 일당증체, 사료효율 및 출하체중에서 각각 10%, 6.8%, 8.2%로서 유의적으로 증가되었으나(P<0.05), 황토의 첨가는 한우의 성장성적에 뚜렷한 효과는 없는 것으로 나타났고, 2%의 첨가수준은 오히려 감소되는 경향을 보였다. 육질 및 경제성 분석에서는 RCT구는 뚜렷한 개선효과를 보였지만, RT구에서는 황토 1%에서만 첨가 효과가 있는 것으로 나타났다. RCT구에서는 1%, 2% 모두가 0%에 비하여 보다 개선된 결과를 나타내었다. 이와 같은 결과들은 한우 비육에 있어서 황토의 첨가는 성장성적에는 뚜렷한 효과가 나타나지 않았지만 육질과 경제적 효과에서는 1%의 첨가는 개선효과가 있는 것으로 사료되며, 고급육 생산을 위한 황토의 첨가시에는 양질의 조사료 보충이 경제적 효과성을 더욱 향상시킬 것으로 사료된다. This study was conducted to estimate the effects of high quality cubed roughage and red clay (0%, 1%, 2%) on growth performance, meat quality and economical benefits. Thirty-six 6 month-old Korean native cattle, averaging 156㎏ of BW, were assigned to six groups in a 2 (rice straw : RT, rice straw+cubed roughage : RCT)× 3 (0, 1, 2% red clay). During experimental period, the high quality cubed roughage added into rice straw treatment (RCT) was superior to the only rice straw treatment (RT) on daily gain and feed efficiency and final weight 10%, 6.8%, 8.2% (P<0.05) respectively. But the addition of red clay did not affect and even 2% addition decreased those parameters. For meat quality and economical analysis, the addition of high quality cubed roughage made an outstanding result and for RT treatment, only 1% red clay indicated good effects while both 1% and 2% red clay were better than 0% for RCT treatment. From these results, it is assumed that the addition of red clay for fattening Korean native cattle has no effects on growth performance but 1% treatment has good results on improving meat quality and economical benefits. It also suggests that the combination high quality roughage and concentrated red clay is beneficial for producing high quality meat in with economical benefits.

사료첨가제로서 황토와 올리고당이 홀스타인 어린송아지의 성장 및 항병력에 미치는 영향

김준식,최윤재,이보균,김명국,홍중산,김윤학,이홍구 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.4

This experiment was conducted to study effects of loess and a form of oligosaccharide (glucosamine-D) as feed additives for 4 days to 2 month-old Holstein calves on the disease resistance and the growth rate. The total weight gains(WG) of test group given 0.04% glucosamine-D and 3% loess as additives were 43.6㎏ and 37.2㎏, respectively, which were 10.1㎏ and 3.6㎏ higher weight gains compared to control group(33.5㎏). The potential influence of loess and glucosamine-D treatment on the distribution of various leukocyte cell types in the serum of test group was tested using flow cytometic method. CD4 antigen positive cells(i.e., T helper cell) were highest in test group feeding 0.04% glucosamine-D, white class II MHC-positive cell type(antigen presenting cells such as macrophages and dendritic cells) was lowest in 6% loess-feeding group. These results suggest that 3% loess and glucosamin-D were more effective as feed additives for 4 days to 2 month-old Holstein calves to increase the disease resistance and the growth rate than those of any other treatments.

Linolenic Acid in Association with Malate or Fumarate Increased CLA Production and Reduced Methane Generation by Rumen Microbes

Li, X.Z.,Choi, S.H.,Jin, G.L.,Yan, C.G.,Long, R.J.,Liang, C.Y.,Song, Man K. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

An in vitro study was conducted to investigate the effect of malate or fumarate on fermentation characteristics, and production of conjugated linoleic acid (CLA) and methane ($CH_4$) by rumen microbes when incubated with linolenic acid (${\alpha}-C_{18:3}$). Sixty milligrams of ${\alpha}-C_{18:3}$ alone (LNA), or ${\alpha}-C_{18:3}$ with 24 mM malic acid (M-LNA) or ${\alpha}-C_{18:3}$ with 24 mM fumaric acid (F-LNA) were added to the 150 ml culture solution consisting of 75 ml strained rumen fluid and 75ml McDougall's artificial saliva. Culture solution for incubation was also made without malate, fumarate and ${\alpha}-C_{18:3}$ (Control). Two grams of feed consisting of 70% concentrate and 30% ground alfalfa (DM basis) were also added to the culture solution of each treatment. In vitro incubation was made anaerobically in a shaking incubator up to 12 h at $39^{\circ}C$. Supplementation of malate (M-LNA) or fumarate (F-LNA) increased pH at 6 h (p<0.01) and 12 h (p<0.001) incubation times compared to control and linolenic acid (LNA) treatments. Both malate and fumarate did not influence the ammonia-N concentration. Concentration of total VFA in culture solution was higher for M-LNA and F-LNA supplementation than for control and LNA treatments from 6 h (p<0.040) to 12 h (p<0.027) incubation times, but was not different between malate and fumarate for all incubation times. Molar proportion of $C_3$ was increased by F-LNA and M-LNA supplementation from 6 h (p<0.0001) to 12 h (p<0.004) incubation times compared to control and LNA treatments. No differences in $C_{3}$ proportion, however, were observed between M-LNA and F-LNA treatments. Accumulated total gas production for 12h incubation was increased (p<0.0002) by M-LNA or F-LNA compared to control or LNA treatment. Accumulated $CH_4$ production for 12 h incubation, however, was greatly reduced (p<0.0002) by supplementing malate or fumarate compared to the control, and its production from M-LNA or F-LNA treatment was smaller than that from LNA treatment. Methane production from LNA, M-LNA or F-LNA treatment was steadily lower (p<0.01 - p<0.001) from 3 h incubation time than that from the control, and was also lower for M-LNA or F-LNA treatment at incubation times of 6 h (p<0.01) and 9 h (p<0.001) than for LNA treatment. Methane production from LNA, however, was reduced (p<0.01 - p<0.001) from 3 h to 9 h incubation times compared to the control. Both malate and fumarate increased concentration of trans11-$C_{18:1}$ from 3 h to 12 h incubation (p<0.01), cis9,trans11-CLA up to 6 h incubation (p<0.01 - p<0.01), trans10,cis12-CLA at 3 h (p<0.05) and 12 h (p<0.01), and total CLA for all incubation times (p<0.05) compared to corresponding values for the ${\alpha}-C_{18:3}$ supplemented treatment (LNA). In conclusion, malate and fumarate rechanneled the metabolic $H_2 pathway to production of propionate and CLA, and depressed the process of biohydrogenation and methane generation. Linolenic acid alone would also be one of the optimistic alternatives to suppress the $CH_4$ generation.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Effect of Stepped Pattern of Feed Intake Using Rice Straw as Roughage Source on Regulation of Growth, Reproduction and Lactation in Dairy Heifers

Jin, M.G.,Lee, H.G.,Lee, H.J.,Hong, Z.S.,Wang, J.H.,Yin, Y.H.,Jin, R.H.,Cho, K.K.,Choi, Y.J. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

An attempt was made to improve the efficiency of growth and lactation performance of dairy heifers subjected to a stair-step growth scheme using rice straw as the sole roughage source. Twenty-four young Holstein heifers were randomly assigned to either control or test group. The control diet met the National Research Council (NRC) requirement, with heifers calving at 24 to 26 mo of age. The test group was individually fed according to a schedule of 3, 2, 4, 2, 5 and 2 mo in which feed intake was alternately 20% below or 25% above the NRC requirements. Heifers on the stair-step growth pattern gained more body weight and consumed less dry matter (10.80 and 11.22%, respectively), resulting an increasing growth efficiency compared with the control. Body condition, first estrus, first conception, services per conception and calving difficulty (data not shown) were not affected. Milk yield of the test group was 8.5% higher than that of the control group. During the early lactation period, the milk yield was significantly higher in the stair-step group than in that of the control group (p<0.05). Milk composition was not affected by compensatory growth induced by the stair-step scheme. Also, weight at calving and calf growth performance was not affected by stair-step growth. The results indicate that using rice straw as a sole roughage source in a stair-step compensatory growth scheme can contribute to the improvement of growth efficiency and early lactation performance.

Upgraded high time-resolved x-ray imaging crystal spectroscopy system for J-TEXT ohmic plasmas (4 pages)

Jin, W.,Chen, Z.Y.,Huang, D.W.,Li, Q.L.,Yan, W.,Luo, Y.H.,Lee, S.G.,Shi, Y.J.,Huang, Y.H.,Tong, R.H. AMERICAN INSTITUTE OF PHYSICS 2014 Review of scientific instruments Vol.85 No.2

Harzianums A and B produced by a fungal strain, Hypocrea sp. F000527, and their cytotoxicity against tumor cell lines

Jin, H.-Z.,Lee, J.-H.,Zhang, W.-D.,Lee, H.-B.,Hong, Y.-S.,Kim, Y.-H.,Lee, J.-J. Taylor Francis 2007 Journal of Asian natural products research Vol.9 No.3

<P> A new compound, harzianum B (1), was isolated from the culture broth of a fungal strain, Hypocrea sp. F000527, together with a known trichothecene, harzianum A (2). Its structure was determined by spectroscopic analyses including HRFAB-MS and various 1H NMR and 13C NMR spectral data. Harzianum B (1) was characterised as (E,Z,E)-2', 4', 6'-octatriendioic acid esterified on the 4&bgr;-hydroxyl group of trichodermol. Harzianums A (2) and B (1) showed cytotoxicity against several human cancer cell lines.</P>

Monitoring of PCBs at facilities related with PCB-containing products and wastes in South Korea

Jin, G.Z.,Fang, M.L.,Kang, J.H.,Park, H.,Lee, S.H.,Chang, Y.S. Elsevier Scientific Pub. Co 2011 Journal of hazardous materials Vol.196 No.-

Polychlorinated biphenyl (PCB) contents were analyzed in samples collected from facilities related to PCB-containing products or wastes in South Korea. Average concentrations of the atmospheric Σ<SUB>209</SUB> PCBs were 7420 (37.0-104,048)pgm<SUP>-3</SUP> and 16.8 (ND-34.2)fgWHO-TEQm<SUP>-3</SUP> in indoor air samples; and 1670 (106-13,382)pgm<SUP>-3</SUP> and 5.64 (ND-36.0)fgWHO-TEQm<SUP>-3</SUP> in outdoor air samples. The highest levels were observed in indoor air samples from disposal facilities (7336-104,048pgm<SUP>-3</SUP>), followed by production (330-25,057pgm<SUP>-3</SUP>), recycling, and storage facilities, indicating that PCB emissions from PCB-containing products and wastes remains very high and the facilities related with those may be an important source to atmospheric PCBs. Principal component analysis of PCB profiles showed that the homologue patterns of PCBs in outdoor and indoor air samples collected from the facilities were similar to those of boundary air samples and PCB commercial products, e.g. Aroclor 1016, 1221, 1232 and 1242. Evaluation of the PCB mass balance in a facility, dismantling and solvent-washing PCB-contaminated transformers, showed that of the total PCBs treated in this facility, approximately 0.0022% was emitted to the atmosphere, and most was transferred to waste oil for disposal by incineration or chemical methods.