Alpha radionuclide-chelated radioimmunotherapy promoters enable local radiotherapy/chemodynamic therapy to discourage cancer progression

Jiajia Zhang,Feize Li,Yuzhen Yin,Ning Liu,Mengqin Zhu,Han Zhang,Weihao Liu,Mengdie Yang,Shanshan Qin,Xin Fan,Yuanyou Yang,Kun Zhang,Fei Yu 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Astatine-211 is an α-emitter with high-energy α-ray and high cytotoxicity for cancer cells. However, the targeted alpha therapy (TAT) also suffers from insufficient systematic immune activation, resulting in tumor metastasis and relapse. Combined immune checkpoint blockade (ICB) with chemodynamic therapy (CDT) could boost antitumor immunity, which may magnify the immune responses of TAT. This study aims to discourage tumor metastasis and relapse by tri-model TAT-CDT-ICB strategy. Methods: We successfully designed Mn-based radioimmunotherapy promoters (211At-ATE-MnO2-BSA), which are consisting of 211At, MnO2 and bovine serum albumin (BSA). The efficacy of 211At-ATE-MnO2-BSA was studied as monotherapy or in combination with anti-PD-L1 in both metastatic and relapse models. The immune effects of radioimmunotherapy promoters on cytotoxic T lymphocytes and dendritic cells (DCs) were analyzed by flow cytometry. Enzymelinked immunosorbent assay and immunofluorescence were used to explore the underlying mechanism. Results: Such radioimmunotherapy promoters could not only enhance the therapeutic outcomes of TAT and CDT, but also induce robust anti-cancer immune activity by activating dendritic cells. More intriguingly, 211At-ATE-MnO2-BSA could effectively suppress the growths of primary tumors and distant tumors when combined with immune checkpoint inhibitors. Conclusions: The tri-model TAT-CDT-ICB strategy provides a long-term immunological memory, which can protect against tumor rechallenge after eliminating original tumors. Therefore, this work presents a novel approach for TATCDT-ICB tri-modal cancer therapy with repressed metastasis and relapse in clinics.

Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana

Zhang, Liang,Meng, Fei,Li, Yuzhen,Kang, Mingjiang,Guo, Xingqi,Xu, Baohua Korean Society for Biochemistry and Molecular Biol 2012 BMB Reports Vol.45 No.5

The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, $Accp38b$, was isolated from $Apis$ $cerana$ $cerana$ and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that $Accp38b$ was induced by multiple stressors. Notably, the expression of $Accp38b$ was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of $A.$ $cerana$ $cerana$. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.

Overexpression of a LAM Domain Containing RNA-Binding Protein LARP1c Induces Precocious Leaf Senescence in Arabidopsis

Bangyue Zhang,Yuzhen Han,Jianheng Jia,Min Yang,Chunxia Yan 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.4

Leaf senescence is the final stage of leaf life history, and it can be regulated by multiple internal and external cues. La-related proteins (LARPs), which contain a well-con-served La motif (LAM) domain and normally a canonical RNA recognition motif (RRM) or noncanonical RRM-like motif, are widely present in eukaryotes. Six LARP genes (LARP1a-1c and LARP6a-6c) are present in Arabidopsis, but their biological functions have not been studied previously. In this study, we investigated the biological roles of LARP1c from the LARP1 family. Constitutive or inducible overexpression of LARP1c caused premature leaf senescence. Expression levels of several senes-cence-asso-ciated genes and defense-related genes were elevated upon overexpression of LARP1c. The LARP1c null mutant 1c-1 impaired ABA-, SA-, and MeJA-induced leaf senescence in detached leaves. Gene expression profiles of LARP1c showed age-dependent expression in rosette leaves. Taken together, our results suggest LARP1c is involved in regulation of leaf senescence.

MAP3Kθ1 is Involved in Abscisic Acid Signaling in Drought Tolerance and Seed Germination in Arabidopsis

Liguo Jia,Yuzhen Chen,Mingshou Fan,Wenrao Li,Jianhua Zhang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.1

Mitogen-activated protein kinase cascades play pivotal roles in mediating environmental stress responses and plant development. In this study, a loss-of-function mutant of Arabidopsis, map3kθ1, exhibited wider stomatal openings, reduced root elongation, and increased seed germination rate compared with its wild type under exogenous abscisic acid (ABA) treatment. MAP3Kθ1 encodes a MAP kinase kinase kinase (MAP3K) with unknown function. Two overexpression lines of MAP3Kθ1 exhibited inhibited seed germination and narrowed stomata, which were aggravated by ABA treatment. Upregulation of MAP3Kθ1 also resulted in stronger drought tolerance, whereas map3kθ1 was more sensitive to water defciency, partially due to diferences in the water-holding capacity of leaves. The MAP3Kθ1-overexpressing lines also showed a greater ability to maintain root elongation under exogenous ABA. Expression of MAP3Kθ1 was inhibited by ABA, H2O2, and methyl viologen treatments in roots. The MAP3Kθ1-overexpressing lines accumulated more ABA by promoting its biogenesis and inhibiting its catabolism, whereas the map3kθ1 mutant accumulated less ABA, compared with wild-type plants. These fndings indicate that MAP3Kθ1 promotes ABA accumulation to regulate stomatal movement, root elongation, and seed germination, while the ABA–H2O2 signaling module inhibits MAP3Kθ1 expression through feedback regulation.

Fabrication and dielectric properties of CCTO/PF dielectric composites: CCTO with different particle size

Yang Zhao,Yuzhen Zhao,Yuhua Niu,Yongming Zhang,Zongcheng Miao 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.3

Calcium copper titanate (CaCu3Ti4O12, CCTO) particles with three types of particle size (CCTO–i, CCTO–ii, CCTO–iii) areprepared by wet chemical method. And then the dielectric composites based on CCTO particles and Phenol formaldehyderesin (PF) are fabricated by mixing and mould pressing. This paper mainly studies the microstructure and dielectric propertiesof CCTO/PF dielectric composites (CCTO–i, CCTO–ii and CCTO–iii composites). XRD, FTIR and SEM indicate that theCCTO particles are dispersed in the polymer matrix. In addition, the effect of particle size on the dielectric properties ofCCTO/PF composites is also discussed. By comparing the CCTO/PF composites with different particle sizes, it is found thatthe CCTO–i composite has a high maximum dielectric constant and low loss at room temperature of 100 Hz, while the CCTOvolume fraction was 0.5 in the measurement frequency range, which shows a very weak frequency dependence. On this basis,the CCTO–i composite with relatively small particle size has excellent dielectric properties, which may imply that the effectof interface will be changed by smaller particle size.

Catalytic Conversion of Cellulose to Cellulose Acetate Propionate (CAP) Over SO₄^2-/ZrO₂ Solid Acid Catalyst

Leng, Yixin,Zhang, Yun,Huang, Chunxiang,Liu, Xiaocheng,Wu, Yuzhen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

The solid super acid catalyst $SO{_4}^{2-}$/$ZrO_2$ was prepared by impregnation method using $ZrO_2$ as the catalyst support. Catalyst forming was taken into consideration in order to separate catalyst from the mixture of cellulose acetate propionate (CAP). $Al_2O_3$ and sesbania gum powder were selected as binding agent and auxiliary agent respectively. The catalytic properties were evaluated through esterification of cellulose with acetic anhydride, propionic anhydride and characterized by XRD, FTIR and $NH_3$-TPD. In this paper, the effects of concentration of $H_2SO_4$ impregnated, calcination temperature, esterification temperature and esterification time on the yield, acyl content and viscosity of CAP were investigated. The results showed that $SO{_4}^{2-}/ZrO_2$ successfully catalyzed CAP synthesis over catalysts impregnated in 0.75 mol/L $H_2SO_4$ and calcined at $500^{\circ}C$. The yield, acetyl content and propionyl content of CAP reached the maximum value of 105.3%, 29.9% and 25.8% reacted at $50^{\circ}C$ for 8 h.

Robustness Analysis for Switched Multiple Equilibrium Points Systems

Zhi Liu,Xian-Fu Zhang,Yuzhen Wang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.6

This article investigates the robustness of switched multiple equilibrium points systems (SMEPSs) withunstable subsystems. Each subsystem has a unique equilibrium point that is different from all equilibrium pointsof the other subsystems. Unlike the most commonly used Lyapunov function method, the analytical solutions andnumerical solutions are calculated to analyze the boundedness of the solutions for perturbation discrete-time andcontinuons-time SMEPSs, respectively. The given perturbation systems have good robustness if the ratio of totaldwell time of unstable subsystems and the total dwell time of stable subsystems is less than or equal to a constant. A numerical example is employed to validate the correctness of the theoretical results.

Catalytic Conversion of Cellulose to Cellulose Acetate Propionate (CAP) Over SO42−/ZrO2 Solid Acid Catalyst

Yixin Leng,Yun Zhang,Chunxiang Huang,Xiaocheng Liu,Yuzhen Wu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

The solid super acid catalyst SO4 2−/ZrO2 was prepared by impregnation method using ZrO2 as the catalyst support. Catalyst forming was taken into consideration in order to separate catalyst from the mixture of cellulose acetate propionate (CAP). Al2O3 and sesbania gum powder were selected as binding agent and auxiliary agent respectively. The catalytic properties were evaluated through esterification of cellulose with acetic anhydride, propionic anhydride and characterized by XRD, FTIR and NH3-TPD. In this paper, the effects of concentration of H2SO4 impregnated, calcination temperature, esterification temperature and esterification time on the yield, acyl content and viscosity of CAP were investigated. The results showed that SO4 2−/ZrO2 successfully catalyzed CAP synthesis over catalysts impregnated in 0.75 mol/L H2SO4 and calcined at 500 oC. The yield, acetyl content and propionyl content of CAP reached the maximum value of 105.3%, 29.9% and 25.8% reacted at 50 oC for 8 h.

A novel hybrid method for robust infrared target detection

( Xin Wang ),( Lingling Xu ),( Yuzhen Zhang ),( Chen Ning ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.10

Effect and robust detection of targets in infrared images has crucial meaning for many applications, such as infrared guidance, early warning, and video surveillance. However, it is not an easy task due to the special characteristics of the infrared images, in which the background clutters are severe and the targets are weak. The recent literature demonstrates that sparse representation can help handle the detection problem, however, the detection performance should be improved. To this end, in this text, a hybrid method based on local sparse representation and contrast is proposed, which can effectively and robustly detect the infrared targets. First, a residual image is calculated based on local sparse representation for the original image, in which the target can be effectively highlighted. Then, a local contrast based method is adopted to compute the target prediction image, in which the background clutters can be highly suppressed. Subsequently, the residual image and the target prediction image are combined together adaptively so as to accurately and robustly locate the targets. Based on a set of comprehensive experiments, our algorithm has demonstrated better performance than other existing alternatives.

Scene-based Nonuniformity Correction Algorithm Based on Temporal Median Filter

Lixiang Geng,Qian Chen,Weixian Qian,Yuzhen Zhang 한국광학회 2013 Current Optics and Photonics Vol.17 No.3

Scene-based nonuniformity correction techniques for infrared focal-plane arrays have been widely considered as a key technology, and various algorithms have been proposed to compensate for fixed-pattern noise. However, the existed algorithms’ capability is always restricted by the problems of convergence speed and ghosting artifacts. In this paper, an effective scene-based nonuniformity correction method is proposed to solve these problems. The algorithm is an improvement over the constant statistics method and a temporal median is utilized with the Gaussian kernel to estimate the nonuniformity parameters. Also theoretical analysis is conducted to demonstrate that effective ghosting artifacts elimination and superior convergence speed can be obtained with the proposed method. Finally, the performance of the proposed technique is tested with infrared image sequences with simulated nonuniformity and with infrared imagery with real nonuniformity. The results show the proposed method is able to estimate each detector’s gain and to offset reliably and that it performs better in increasing convergence speed and reducing ghosting artifacts compared with the conventional techniques.