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Seong, Yunseo,Yoo, Young Sook,Akter, Hafeza,Kang, Min-Jung Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1060 No.-
<P><B>Abstract</B></P> <P>Proteomics is a valuable approach to discover biomarkers in human plasma for early diagnosis. However, detection of biomarkers in the plasma is still challenging because of its large protein content. In our study, we established albumin/IgG depletion methods for identification of low abundance proteins using two commercial kits with additional buffer conditions and various concentrations of cold acetone. Trypsin digestion, desalting, and data-dependent acquisition were also optimized. More than 80% depletion of albumin/IgG was achieved with two commercial kits and 98% depletion of albumin was obtained with 70% cold acetone. Recovery of four reference proteins, BNP (47–76), insulin, cytochrome c, and ubiquitin was obtained in all optimized methods. The best recovery of reference proteins was obtained using the ProteoExtract albumin/IgG removal kit with buffer A (61%–106%). After cold acetone precipitation, three reference proteins were recovered more than 48% except ubiquitin (12%). The number of identified proteins by Mascot was 28, 35, 17, and 34 for ProteoExtract, ProteoPrep, 70%, and 50% cold acetone, respectively. Furthermore, optimized methods detected MS/MS fragmentation patterns of elevated BNP in patient samples with cardiac disease. Our study provides the conditions for efficient biomarker discovery by minimal removing of high abundant proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Low abundance proteins as biomarker candidates were detected after simple sample preparation. </LI> <LI> UHPLC-MS/MS conditions and Mascot parameters were optimized for low abundance biomarker discovery. </LI> <LI> The established method was practically applied to discover BNP fragments from patient plasma samples with AMI. </LI> </UL> </P>
Ultrasonic MQL System for Generating Size-controlled Droplets
Axel Lefebure,Seeun Kim,Yunseo Kang,Hajin Park,Jumi Lee,Seohee Jang,Inho Lee,Juhye Shin,Chang Soo Lee,Deuk Kwi Lee,Young Chang Park,Jin Hur,Hyung Jin Kwon,Dongha Shim 한국생산제조학회 2021 한국생산제조학회지 Vol.30 No.5
We present an ultrasonic MQL system for generating size-controlled droplets, engaging the requirement of variations in MQL droplet size depending on the used machining process and material. The proposed system architecture features four series chambers with a different vibrating mesh actuator; it demonstrates that a wide range of material- and machining process-specific lubrication requirements can be achieved by varying the mist atomization rate and droplet diameter. The vibrating-mesh outlet pore sizes were differentiated between 2 to 20 μm and driven by a 100 V peak-to-peak bipolar sinusoidal voltage. The oil with a kinematic viscosity of 8.9 cSt contacted the atomizing surface using an FLDC-configuration. Four-size mists were generated with mean volumetric droplet diameters of 19.1, 21.7, 39.0 and 48.3 μm; their atomization rates vary between 1.3 to 39.4 ml/h. The droplet size control must provide more flexibility for emerging ultrasonic MQL systems with improved lubrication and optimized machine-working performance.
강민정 ( Min-jung Kang ),성윤서 ( Yunseo Seong ),김문주 ( Moon-ju Kim ),김명수 ( Myung Soo Kim ),변재철 ( Jae-chul Pyun ) 한국공업화학회 2017 공업화학 Vol.28 No.3
최근에, 질량분석기술의 폴리머 분석에의 응용은 MALDI-TOF MS 개발 이후 급속도로 발전하였다. 이 리뷰 논문은 현재까지 연구된 MALDI-TOF MS의 폴리머 특성분석에의 응용에 관한 최신 논문을 정리하였다. MALDI-TOF MS는 바이오 폴리머와, 합성 폴리머의 평균분자량 분석, 폴리머의 시퀀스 분석을 통한 구조의 해석, 모노머의 조성분석에까지 이용되고 있다. 엔드그룹의 특성과 농도를 분석하는 연구도 많이 진행되었고, 복잡한 폴리머의 분자량의 분석에는 SEC와 MALDI-TOF MS를 연결한 분석법을 추천한다. MALDI에 tandem MS를 결합한 분석기술이나, 이온 모빌리티를 응용한 질량분석기, TOF-SIMS, MALDI-TOF-Imaging 기술도 급격히 발전하고 있으며, 이의 폴리머 특성분석에의 응용은 별도의 분리기술이 필요 없어 앞으로 더 많이 이용될 것으로 생각된다. 분자량, 시퀀스, 그리고 모노머의 조성을 정확하게 계산해주는 소프트웨어와 고분자량(> 100 kDa)의 분석을 가능하게 해주는 기술이 개발된다면, 폴리머를 연구하는 과학자들에게 MALDI-TOF MS의 이용은 문제점을 해결하고, 목적하는 폴리머를 합성하는 데 중요한 수단이 될 것이다. The application of mass spectrometry to polymer science has rapidly increased since the development of MALDI-TOF MS. This review summarizes current polymer analysis methods using MALDI-TOF MS, which has been extensively applied to analyze the average molecular weight of biopolymers and synthetic polymers. Polymer sequences have also been analyzed to reveal the structures and composition of monomers. In addition, the analysis of unknown end-groups and the determination of polymer concentrations are very important applications. Hyphenated techniques using MALDI-tandem MS have been used for the analysis of fragmentation patterns and end-groups, and also the combination of SEC and MALDI-TOF MS techniques is recommended for the analysis of complex polymers. Moreover, MALDI-TOF MS has been utilized for the observation of polymer degradation. Ion mobility MS, TOF-SIMS, and MALDI-TOF-imaging are also emerging technologies for polymer characterization because of their ability to automatically fractionate and localize polymer samples. The determination of polymer characteristics and their relation to the material properties is one of the most important demands for polymer scientists; the development of software and instrument for higher molecular mass range (> 100 kD) will increase the applications of MALDI-TOF MS for polymer scientists.