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Sc₂O₃를 첨가한 SnO₂/Ca,Pt 가스센서의 부탄감응 특성
이윤수,성정훈,강봉휘,서무교,이덕동 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1
A coprecipitation method was used for preparing Ca and Pt doped SnO_(2) powder. Components were investigated by XRD and SEM. Sc_(2)O_(3) added SnO_(2) /Ca,Pt thick film devices were fabricated by screen printing technique for butane gas detecting. By addition of Sc_(2)O_(3), the selectivity of SnO_(2) /Ca,Pt thick film for butane gas was enhanced. The sensitivity of Sc_(2)O_(3) added SnO_(2) /Ca,Pt device to 3,000 ppm butane in air was about 90% at the operating temperature. of 200℃. And the device showed good selectivity for butane gas.
Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata
Lee,Kang,In,Jun-Gyo,Yu,Chang-Yeon,Yun,Song-Joong,Min,Byung-Hoon,Rho,Yeong-Deok,Kim,Moo-Sung,Yang,Deok-Chun 한국자원식물학회 2004 Plant Resources Vol.7 No.3
To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.
송갑득,박영일,이상문,이윤수,최낙진,주병수,서무교,허증수,이덕동 한국센서학회 2002 센서학회지 Vol.11 No.6
센서의 안정도와 감도를 개선시킬 수 있는 단일전극을 가진 열선형 마이크로 가스센서를 제작하였다. 일반적으로, 금속산화물 반도체를 이용한 가스센서는 히터전극과 감지전극의 두 개의 전극을 가지고 있다. 제작된 센서는 히터전극위에 감지물질을 형성하여 단일 전극을 가지는 구조를 가지고 있다. 히터와 감지전극으로 사용되는 Pt는 glass 기판위에 스퍼터링법으로 형성하였으며, SnO_2 감지물질은 제작된 Pt 전극위에 열증착시켜 형성하였다. SnO_2막은 lift-off 공정을 이용해서 패턴을 형성하고 1시간 동안 산소분위기에서 열산화하였다. 제작된 소자의 크기는 1.92x.1 mm^2 이다. CO 가스에 대한 감지특성을 조사한 결과 1,000 ppm에 대해 100 mV의 출력변화를 나타내었으며, 넓은 농도범위(0 ~ 10,000 ppm에서 선형적인 전압출력을 나타내었다. 또한 가스 반응 전과 반응 후의 전압출력을 비교해 볼 때, 1 % 이내의 편차를 나타내는 우수한 회복성을 나타내었다. Micro gas sensor with single electrode was proposed for improving stability and sensitivity, Generally, metal oxide gas sensors have two electrodes for heating and sensing. This fabricated new type sensor have only a single electrode by forming a sensing material onto heating electrode. Pt as a heating and sensing electrode was sputtered on glass and a SnO_2 sensing material was thermally evaporated on Pt electrode. SnO_2 was patterned by lift-off process and then thermally oxidized in O_2 condition for 1 hr., 600 ℃. The size fabricated sensor was 1.9 x 2.1 mm^2. As a result of CO gas sensing characteristics, this sensor showed 100 mV change for 1,000 ppm and linearlity for wide range(0 ~ 10,000 ppm) of gas concentrations. And the sensor shows a good recovery characteristics of 1 % deviation compared to initial resistance.
Lee, Yun Sun,Park, Hyun-Seung,Lee, Dong-Kyu,Jayakodi, Murukarthick,Kim, Nam-Hoon,Lee, Sang-Choon,Kundu, Atreyee,Lee, Dong-Yup,Kim, Young Chang,In, Jun Gyo,Kwon, Sung Won,Yang, Tae-Jin The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.1
Background: Various Panax ginseng cultivars exhibit a range of diversity for morphological and physiological traits. However, there are few studies on diversity of metabolic profiles and genetic background to understand the complex metabolic pathway in ginseng. Methods: To understand the complex metabolic pathway and related genes in ginseng, we tried to conduct integrated analysis of primary metabolite profiles and related gene expression using five ginseng cultivars showing different morphology. We investigated primary metabolite profiles via gas chromatography-mass spectrometry (GC-MS) and analyzed transcriptomes by Illumina sequencing using adventitious roots grown under the same conditions to elucidate the differences in metabolism underlying such genetic diversity. Results: GC-MS analysis revealed that primary metabolite profiling allowed us to classify the five cultivars into three independent groups and the grouping was also explained by eight major primary metabolites as biomarkers. We selected three cultivars (Chunpoong, Cheongsun, and Sunhyang) to represent each group and analyzed their transcriptomes. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Conclusion: Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.
Lee, Jang-Ho,Lee, Joon-Soo,Kwon, Woo-Saeng,Kang, Je-Yong,Lee, Dong-Yun,In, Jun-Gyo,Kim, Yun-Soo,Seo, Jiho,Baeg, In-Ho,Chang, Il-Moo,Grainger, Keith The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.2
Background: Ginseng (Panax ginseng Meyer) is an important medicinal herbs in Asia. However, ginseng varieties are less developed. Method: To developed ginseng varieties, a pure line selection method was applied in this study. Results: Gumpoong was testing of 4-yr-old specimens in 2002, the proportions of the below-ground roots that were rusty colored for Gumpoong was 1.29 in Daejeon and 1.45 in Eumseong, whereas the proportions for its yellow berry variant were 2.60 and 2.45 in the two regions, respectively. Thus the Gumpoong was resistant to root rust. Sunpoong has a high yielding property. Its average root weight is 70.6 g for 6-yr-old roots. Its yield is 2.9 kg/$1.62m^2$ and the rate of heaven- and earth-grade product is 20.9%, which is very high compared to 9.4% for Yunpoong. Sunone is resistance to root rot and the survival rate of 4-yr-old roots was 44.4% in 1997, whereas that of the violet-stem variant landrace was 21.7%. Sunhyang has content of arginyl-fructosyl-glucose (AFG), which produces the unique scent of red ginseng, is $95.1{\mu}mol/g$ and greater than the $30.8{\mu}mol/g$ of Chunpoong in 6-yr-old plants. Sunun and Cheongsun are being nurtured to protect genetic resources. Conclusion: Developed ginsneg varieties will be used as the basis for the protection of genetic resources and breeding.
Phenological growth stages of Korean ginseng (Panax ginseng) according to the extended BBCH scale
Yun-Soo Kim,Chol-Soo Park,Dong-Yun Lee,Joon-Soo Lee,Seung-Hwan Lee,Jun-Gyo In,Tae-Kyun Hong 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.4
Background: Phenological studies are a prerequisite for accomplishing higher productivity and better crop quality in cultivated plants. However, there are no phenological studies on Panax ginseng that improve its production yield. This study aims to redefine the phenological growth stages of P. ginseng based on the existing Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie (BBCH) scale and proposes a disease control reference. Methods: This study was conducted at the Korea Ginseng Corporation Experiment Station in Gyeonggi province, South Korea. Phenological observations were performed once weekly or twice monthly, based on the developmental stages. The existing BBCH scale with a three-digit code was used to redefine and update P. ginseng’s phenological growth codes. Results: The phenological description is divided into eight principal growth stages: three for vegetative growth (perennating bud, aerial shoot, and root development), four for reproductive growth (reproductive organ development, flowering, fruit development, and fruit maturation), and one for senescence according to the extended BBCH scale. A total of 58 secondary growth stages were described within the eight principal growth stages. Under each secondary growth stage, four mesostages are also taken into account, which contains the distinct patterns of the phenological characteristics in ginseng varieties and the process of transplanting seedlings. A practical management program for disease control was also proposed by using the BBCH code and the phenological data proposed in this work. Conclusion: The study introduces an extended BBCH scale for the phenological research of P. ginseng.
GSH-dependent peroxidase activity of the rice (Oryza sativa) glutaredoxin, a thioltransferase
Lee, Kyun-Oh,Lee, Jung-Ro,Yoo, Ji-Young,Jang, Ho-Hee,Moon, Jeong-Chan,Jung, Bae-Gyo,Chi, Yong-Hun,Park, Soo-Kwon,Lee, Seung-Sik,Lim, Chae-Oh,Yun, Dae-Jin,Cho, Moo-Je,Lee, Sang-Yeol Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
Glutaredoxin (Grx) is a 12-kDa thioltransoferase that reduces disulfide bonds of other proteins and maintains the redox potential of cells. In addition to its oxidoreductase activity, we report here that a rice Grx(OsGrx) can also function as a GSH-dependent peroxidase. Because of this antioxidant activity, OsGrx protects glutamine synthetase from oxidative damage. Individually replacing the conserved Cys residues in OsGrx with Ser shows that Cys^(23), but not Cys^(26), is essential for the thioltransferase and GSH-de-pendent peroxidase activities. Kinetic Characterization of OsGrx reveals that the maximal catalytic efficiency (V_(max)/K_(m))is obtained with cumene hydroperoxide rather than H_(2)O_(2) or t-butyl hydroperoxide.
중등증의 혈우병 B 보인자 환자에서의 반복 제왕절개술 시행
이대남 ( Lee Dae Nam ),오유진 ( O Yu Jin ),경영수 ( Gyeong Yeong Su ),이다연 ( Lee Da Yeon ),윤선웅 ( Yun Seon Ung ),최중섭 ( Choe Jung Seob ),김계현 ( Kim Gye Hyeon ),이교원 ( Lee Gyo Won ),김성도 ( Kim Seong Do ),한종설 ( Han Jo 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.11
Hemophilia is a hemorrhagic disease caused by the defect or abnormal activities of the coagulation factor. The proper dosage and interval of factor replacement therapy are not defined. So the dosage and interval can be different in each case. We have expe