MiR-182-5p Knockdown Targeting PTEN Inhibits Cell Proliferation and Invasion of Breast Cancer Cells

Yue-Sheng Zhao,Wei-Chao Yang,Hong-Wei Xin,Ji-Xia Han,Su-Gang Ma 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.2

Purpose: Breast cancer (BC) is one of the most common malignant tumors, affecting a significant number of women worldwide. MicroRNAs (miRNAs) have been reported to play important roles in tumorigenesis. The aim of this study was to determine theroles of miR-182-5p in BC progression. Materials and Methods: The expressions of miR-182-5p and phosphatase and tensin homolog deleted on chromosome 10(PTEN) were measured in BC tissues and cells by quantitative real-time polymerase chain reaction or Western blot. Cell proliferationand invasion were detected by cell counting kit-8 assay and trans-well assay, respectively. The interaction between miR-182-5p and PTEN was probed by bioinformatics analysis, luciferase activity, and RNA immunoprecipitation. A murine xenograftmodel was established to investigate the role of miR-182-5p in BC progression in vivo. Results: An abundance of miR-182-5p was noted in BC tissues and cells. High expression of miR-182-5p was associated with poorsurvival. Abrogation of miR-182-5p inhibited cell proliferation and invasion in BC cells. Interestingly, PTEN was indicated as atarget of miR-182-5p, and its restoration reversed miR-182-5p-mediated promotion of proliferation and invasion of BC cells. Moreover, depletion of miR-182-5p suppressed tumor growth via up-regulating PTEN expression in the murine xenograft model. Conclusion: MiR-182-5p exhaustion blocked cell proliferation and invasion by regulating PTEN expression, providing a noveltherapeutic avenue for treatment of BC.

Luminescence and photoconductivity properties of porous polycrystalline silicon

Yue Zhao,Dong-sheng Li,Jie Zhao,Wen-bin Sang,De-ren Yang,Min-hua Jiang 한국물리학회 2008 Current Applied Physics Vol.8 No.2

In recent years, the photoluminescence and the photoconductivity of porous silicon were comprehensively studied. But the photoluminescence and the photoconductivity of porous polycrystalline silicon have not been wholely studied. In this paper, the results showed that luminescent property of the samples prepared by poly-crystal silicon wafers may be related to the defects on Si complexes surface, which can be proved by microwave-detected photoconductivity decay measurements. Furthermore, the luminescence of samples was disappeared under the external illumination, which may be related to the elimination of luminescent-centers. In addition, the conductivities of the samples were dependent on etched time and current density, and the large porosity of samples led to isotropic photoconductivity, which may be related to the change of energy band structure of the devices. In recent years, the photoluminescence and the photoconductivity of porous silicon were comprehensively studied. But the photoluminescence and the photoconductivity of porous polycrystalline silicon have not been wholely studied. In this paper, the results showed that luminescent property of the samples prepared by poly-crystal silicon wafers may be related to the defects on Si complexes surface, which can be proved by microwave-detected photoconductivity decay measurements. Furthermore, the luminescence of samples was disappeared under the external illumination, which may be related to the elimination of luminescent-centers. In addition, the conductivities of the samples were dependent on etched time and current density, and the large porosity of samples led to isotropic photoconductivity, which may be related to the change of energy band structure of the devices.

Synthesis and Cytotoxicity of Novel 3-Amino-4-indolylmaleimide Derivatives

Sheng-Yin Zhao,Yan-Wu Yang,Hai-Quan Zhang,Yun Yue,Mei Fan 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4

In an attempt to develop potent and selective antitumor agents, a series of novel 3-amino-4-indolylmaleimides were designed and synthesized. The reaction showed high regioselectivity. The structure of compound 7a was determined by an X-ray single crystal diffraction method. The cytotoxicities of the title compounds were evaluated against HeLa, SMMC 7721 and HL 60 cancer cell lines by a standard MTT assay in vitro. The pharmacological results showed that some of the title compounds displayed moderate or high cytotoxic activity against the tested cell lines. Compound 7d was the most promising compound against the tested cancer cell lines. Structure-activity relationships are discussed based on the experimental data obtained. A hydroxyethylamino group at the 3-position in the side chain of indolylmaleimide is associated with an increase in cytotoxicity.

Carboxymethyl Flavonoids and A Monoterpene Glucoside from Selaginella moellendorffii

Hong-Sheng Wang,Ling Sun,Yue-Hu Wang,Ya-Na Shi,Gui-Hua Tang,Fu-Wei Zhao,Hong-Mei Niu,Chun-Lin Long,Ling Li 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.8

A new dihydroflavone, 5-carboxymethyl-7,4'-dihydroxyflavonone (1), and its glucoside 5-carboxymethyl-7,4'-dihydroxyflavonone-7-O-β-D-glucopyranoside (2), and one new monoterpene glucoside, (4Z,6E)-2,7-dimethyl-8-hydroxyocta-4,6-dienoic acid 8-O-β-D-glucopyranoside (3), were isolated from the whole plants of Selaginella moellendorffii. Their structures were determined by spectroscopic methods and chemical transformation. Compound 2 was evaluated for the ability to enhance glucose consumption in normal and insulin-resistant L6 muscle cells induced by high concentrations of insulin and glucose. Glucose consumption in insulin-resistant cells (but not in normal cells) was increased 15.2 ± 3.3% (p < 0.01) by compound 2 at a concentration of 0.1 μM in the presence of insulin (1 nM).

Study of the Optimal Film Structure by Using Conventional Elipsometry

Liang-Yao Chen,Ming-Yu Sheng,Yun-Hua Wu,Yuan Zhao,Yue-Rui Chen,Yu-Xiang Zheng 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2

The optimal film structure affected by the space effect has been studied for SiO2 film samples deposited onto Si wafers by using the e-beam evaporation method. The ellipsometric data were measured at different incidence angles and in the 300-800-nm wavelength range. In the range where the phase delay δ is close to π, the film thickness deduced from the conventional ellipsometry model varied with the phase delay. The optimal SiO2 film-thickness was achieved with a modified ellipsometric model, including the space effect. The results given in this work can generally be applied to other optical measurement methods used to study film structures.

Morphology control of mesoporous Cu₂O by reductants and its photocatalytic activity

Chu, Xiao-Zhong,Cheng, Zhi-Peng,Zhao, Yi-Jiang,Xu, Ji-Ming,Li, Mei-Sheng,Hu, Lei,Zhou, Shou-Yong,Wu, Fei-Yue,Lee, Chang-Ha Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.11

<P><B>Abstract</B></P> <P>Mesoporous Cu<SUB>2</SUB>O particles with different morphologies were synthesized via simple one-pot reactions. Effects of reducing agents, dispersant, template, and temperature on the <B>structure</B> of the prepared materials were investigated. Mesoporous flower-shaped Cu<SUB>2</SUB>O materials were obtained at 70℃ using glucose <B>as a reductant</B>. Different hollow microsphere shapes of Cu<SUB>2</SUB>O could be prepared at 40℃ using <B>another type of reductant, ascorbic acid</B>. The results indicated that the uniform morphology of synthesized Cu<SUB>2</SUB>O with mesopores presented a maximum specific surface area of 45.3m<SUP>2</SUP>/g. Furthermore, the as-prepared Cu<SUB>2</SUB>O particles showed good photodegradation efficiencies of methyl orange in the range of 86.0–93.7%, depending on their morphologies.</P>

Metal Adsorbate-Induced Plasmon Damping in Gold Nanorods: The Difference Between Metals

Pengyu Xu,Xuxing Lu,Junwei Zhao,Yue Li,Sheng Chen,Junfei Xue,Weihui Ou,Song Han,YAPING DING,Weihai Ni 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.9

We presented a single particle study on the metal adsorbate-induced plasmon damping in Au nanorods (AuNRs) through adsorbing clusters of different metals including Pt, Au and Ag. AuNRs with different longitudinal surface plasmon resonance (LSPR) wavelength were measured and investigated individually. Linewidth broadening, plasmon shift and reduction of plasmonic resonance of single AuNRs were studied and compared between Pt, Au and Ag adsorbates. The measured linewidths perfectly match the theoretical predictions of the billiard model with increased scattering coefficients resulted from the metal adsorbates. The results indicate that the plasmon damping in case of Ag is significantly weaker than Pt and Au, which can be attributed to longer relaxation time of free electrons in Ag and therefore less loss of the oscillating plasmon electrons. In contrast to the red shift observed from Au and Pt, blue shift of the LSPR is observed in case of Ag. It suggests that plasmonic properties brought by the metal adsorbates can exert dramatic influence on the nanoparticle that is adsorbed with. We believe that our study not only provides important understanding on plasmon damping but pave the road for the fabrication of complex nanostructures with two or more metal elements.