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Yue Huo,Jong Pyo Kang,Jong Chan Ahn,Yeon Ju Kim,Chun Hong Piao,Dong Uk Yang,Deok Chun Yang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2
Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119<SUP>T</SUP> had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10<SUP>8</SUP> CFU/mL DCY119<SUP>T</SUP> for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119<SUP>T</SUP> can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119<SUP>T</SUP> can potentially be used for bioremediation of HM contamination.
Huo, Yue,Kang, Jong-Pyo,Kim, Yeon-Ju,Yang, Deok-Chun Springer-Verlag 2018 Archives of microbiology Vol.200 No.8
<P>The novel species DCY115(T) was isolated from ginseng-cultivated soil in Gochang province, Republic of Korea. The isolated strain was assigned to the genus Paraburkholderia due to its 16S rRNA gene sequence proximity to Paraburkholderia xenovorans LB400(T) (98.8%), Paraburkholderia terricola LMG 20594(T) (98.4%), Paraburkholderia graminis C4D1M(T) (98.2%), Paraburkholderia rhynchosiae WSM3937(T) (98.1%), and Paraburkholderia phytofirmans PsJN(T) (98.1%). Strain DCY115(T) is gram-negative, facultative aerobic, rod-shaped, non-motile, non-flagellated, and oxidase and catalase positive. The predominant isoprenoid quinone of DCY115(T) is ubiquinone Q-8. The major cellular fatty acids are C-16(:0), cyclo-C-17(:0), cyclo-C-19:0 omega 8c, summed feature 3 (C-16(:1) omega 7 c and/or C-16(:1) omega 6c) and summed feature 8 (C-18(:1) omega 7c and/or C-18(:1) omega 6c). The major polar lipids include diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1). The genomic DNA G+ C content is 61.3 mol%. Phenotypic tests and chemotaxonomic analysis place strain DCY115(T) in the genus Paraburkholderia. DNA-DNA hybridization values between strain DCY115(T) and closely related reference strains were lower than 51%. The low DNA relatedness data in combination with phylogenetic and biochemical tests showed that strain DCY115(T) could not be assigned to any recognized species. Finally, strain DCY115 T showed antagonistic activity against Fusarium solani (KACC 44891(T)) and Cylindrocarpon destructans (KACC 44660(T)), which are two root rot fungal pathogens of ginseng. In conclusion, the results in this study support strain DCY115(T) as a novel species within the genus Paraburkholderia for which the name Paraburkholderia panacihumi is proposed. The type strain is DCY115(T) (=KCTC 52952(T)=JCM 32099(T)).</P>
Huo, Yue,Kang, Jong-Pyo,Park, Jin-Kyu,Li, Jinfeng,Chen, Ling,Yang, Deok-Chun Springer-Verlag 2018 Archives of microbiology Vol.200 No.10
<P>A novel bacterium, designated DCY112(T), was isolated from the rhizospheric soil of a ginseng-cultivated field in Gochang-gun, Republic of Korea. Based on 16S rRNA gene sequence analysis, this isolate was assigned to the genus Rhodanobacter and is closely related to Rhodanobacter soli DCY45(T) (98.0%) and R. umsongensis GR24-2(T) (98.0%). Strain DCY112(T) is Gram-negative, catalase- and oxidase-positive, aerobic, non-motile, rod-shaped, and produces yellow-pigmented colonies on R2A medium. Q-8 was the predominant respiratory quinone. The major cellular fatty acids were iso-C-15:0, iso-C-17:0, and summed feature 9 (iso-C(17:1)9c and/or 10-methyl-C-16:0). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown amino lipid (AL1), and an unidentified polar lipid (L3). The genomic DNA G+C content was 65.2 mol%. DNA-DNA homology values between strain DCY112(T) and related strains were lower than 55%. The low DNA relatedness data in combination with phenotypic and genotypic tests indicated that strain DCY112(T) could not be assigned to a recognized species. Strain DCY112(T) showed antagonistic activity against the fungal pathogen Fusarium solani (KACC 44891(T)), which causes ginseng root rot. The results of this study support that strain DCY112(T) is a novel species belonging to the genus Rhodanobacter, for which the name Rhodanobacter ginsengiterrae is proposed. The type strain is DCY112(T) (=KCTC 62018(T)=JCM 32167(T)).</P>
Lysobacter panacihumi sp. nov., isolated from ginseng cultivated soil
HUO YUE,강종표,Joon Hurh,Yaxi Han,안종찬,MATHIYALAGANRAMYA,Chunhong Piao,양덕춘 한국미생물학회 2018 The journal of microbiology Vol.56 No.10
A Gram-negative, non-motile, aerobic, catalase-, and oxidasepositive bacterial strain, designated DCY117T, was isolated from ginseng cultivated soil in Gochang-gun, Republic of Korea, and was characterized taxonomically using a multifaceted approach. 16S rRNA gene sequence analysis revealed that strain DCY117T showed highest similarity to Lysobacter ruishenii CTN-1T (95.3%). Phylogenetic analysis revealed that closely related relatives of strain DCY117T were L. aestuarii S2-CT (95.1%), L. daejeonensis GH1-9T (95.0%), and L. caeni BUT-8T (94.9%). Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) were the major polar lipids of strain DCY117T. The major isoprenoid quinone was Q-8. The major cellular fatty acids of strain DCY117T were iso-C15:0, iso-C16:0, and summed feature 9 (comprising iso-C17:1 ω9c and/or 10-methyl-C16:0). Genomic DNA G + C content was 61.8 mol%. On the basis of our findings, strain DCY117T is a novel species in the genus Lysobacter. We propose the name Lysobacter panacihumi sp. nov., and the type strain is DCY117T (= KCTC 62019T = JCM 32168T).
근부병원균에 길항적인 인삼재배 흙으로부터 분리된 Paraburkholderia panacihumi sp. nov.
Yue Huo,강종표,안종찬,박진규,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Plants cultivation is hindered by root rot, a major disease caused by the soil-born fungi. The ginseng-cultivated soil is one of the nutritious habitats for soil-borne microorganisms. Bacteria from ginseng-cultivated soil can increase plant growth by supplying nutrients and hormones as well as protecting against pathogenic fungal infections and induced systematic resistance. Methods and Results : The novel species DCY115T was isolated from ginseng-cultivated soil in Gochang province, Republic of Korea. The isolate was assigned to the genus Paraburkholderia due to its 16S rRNA gene sequence closely proximity to P. xenovorans LB400T (98.8%). Strain DCY115T is gram-negative, facultative aerobic, rod-shaped, non-flagellated, oxidase and catalase positive. The predominant isoprenoid quinone is ubiquinone Q-8. The genomic DNA G + C content is 61.3 mol%. Phenotypic tests and chemotaxonomic analysis place strain DCY115T in the genus Paraburkholderia. DNA-DNA hybridization values between strain DCY115T and closely related reference strains were lower than 51%. The DNA relatedness data in combination with phylogenetic and biochemical tests showed that strain DCY115T could not be assigned to any recognized species. Finally, strain DCY115T showed the plant growth promoting activities of siderophores production, phosphate solubilization, and antagonistic activity against root rot fungal pathogen Fusarium solani (KACC 44891T) and Cylindrocarpon destructans (KACC 44660T). Conclusion : The results support the novel strain DCY115T as a potential biocontrol agent against root rot fungal pathogen within the genus Paraburkholderia for which the name Paraburkholderia panacihumi is proposed. The type strain is DCY115T (= KCTC52952T = JCM32099T).
Xing Yue Xu,Eun Seob Yi,Chang Ho Kang,Ying Liu,Yeong-Geun Lee,Han Sol Choi,Hyun Bin Jang,Yue Huo,Nam In Baek,Deok Chun Yang,Yeon Ju Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6
Background: Main bioactive constituents and pharmacological functions of ripened red ginseng berry(Panax ginseng Meyer) have been frequently reported. Yet, the research gap targeting the beneficial activitiesof transformed green ginseng berries has not reported elsewhere. Methods: Ginsenosides of new green berry cultivar K-1 (GK-1) were identified by HPLC-QTOF/MS. Ginsenosidesbioconversion in GK-1 by bgp1 enzyme was confirmed with HPLC and TLC. Then, mechanismsof GK-1 and b-glucosidase (bgp1) biotransformed GK-1 (BGK-1) were determined by QuantitativeReverse Transcription-Polymerase Chain Reaction and Western blot. Results: GK-1 possesses highest ginsenosides especially ginsenoside-Re amongst seven ginseng cultivarsincluding (Chunpoong, Huangsuk, Kumpoong, K-1, Honkaejong, Gopoong, and Yunpoong). Ginseng root’sbiomass is not affected with the harvest of GK-1 at 3 weeks after flowering period. Then, Re is bioconvertedinto a promising pharmaceutical effect of Rg2 via bgp1. According to the results of cell assays,BGK-1 shows decrease of tyrosinase and melanin content in a-melanocyte-stimulating hormonechallenged-murine melanoma B16 cells. BGK-1 which is comparatively more effective than GK-1 extractshows significant suppression of the nuclear factor (NF)-kB activation and inflammatory target genes, inLPS-stimulated RAW 264.7 cells. Conclusion: These results reported effective whitening and anti-inflammatory of BGK-1 as compared toGK-1.
A Ratiometric Fluorescent Assay for Fluazinam Based on FRET Between CdTe Quantum Dots and Porphyrin
Yue Wang,DANQUN HUO,Huixiang Wu,Hui Liu,Junjie Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.10
A fluorescent detection system for fluazinam was reported using fluorescence resonance energy transfer (FRET) method based on CdTe quantum dots (CdTe QDs) and 5, 10, 15, 20-tetrakis(4-methacryloyloxy) phenyl porphyrin (TMaPP). TMaPP and water-soluble CdTe QDs were synthesized successfully and characterized using FT-IR, 1H NMR, XPS and TEM, respectively. FRET mechanism between CdTe QDs and TMaPP was confirmed by detailed studies on their fluorescent spectra. After a co-culture of TMaPP and CdTe QDs, fluorescent intensity of CdTe QDs decreased significantly while that of TMaPP increased concomitantly due to altered FRET. Addition of fluazinam led to impaired energy transfer from CdTe QDs to TMaPP and therefore fluorescence recovery of CdTe QDs with fluorescence quenching of TMaPP. The correlation of fluazinam concentration with the fluorescence intensity ratio FQDs / FTMaPP provided the basis for quantitative analysis, and a broad linear range varying from 0.01 μM to 5 μM with a low detection limit of 2.3 nM was obtained. As-reported sensor system demonstrated excellent reproducibility, selectivity and sensitivity in real sample detection.