Rice C2-Domain Proteins Are Induced and Translocated to the Plasma Membrane in Response to a Fungal Elicitor

Kim, Cha-Young,Koo, Yoon-Duck,Jin, Jing-Bo,Moon, Byeong-Cheol,Kang, Chang-Ho,Kim, Sun-Tae,Park, Byung-Ouk,Lee, So-Young,Kim, Man-Lyang,Hwang, In-Hwan,Kang, Kyu-Young,Bahk, Jeong-Dong,Lee, Sang-Yeol,,C Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

ABSTRACT: Hundreds of proteins involved in signaling pathways contain a Ca^(2+)-dependent membrane-binding motif called the C2-domain. However, no small C2-domain proteins consisting of a single C2-domain have been reported in animal cells. We have isolated two cDNA clones, OsERG1a and OsERGIb, that encode two small C2-domain proteins of 156 and 159 amino acids, respectively, from a fungal elicitor-treated rice cDNA library. The clones are believed to have originated from a single gene by alternative splicing. Transcript levels of the OsERG1 gene are dramatically elvated by a fungal elicitor prepared from Magnaporthe grisea or by Ca^(2+) ions. The OsERG1 protein produced in Escherichia coli binds to phospholipid vesicles in a Ca^(2+)-dependent manner and is translocated to the plasma membrane of plant cells by treatment with either a fungal elicitor of a Ca^(2+)-ionophore. These results suggest that OsERG1 proteins containing a single C2-domain are involved in plant defense signaling systems.

오대산 소나무림의 유기탄소 분포 및 수지

장미나 ( Mi Na Jang ),김성용 ( Sung Yong Kim ),( Azyleah C. Abino ),김창현 ( Chang Hyun Kim ),이중효 ( Jung Hyo Lee ),이영진 ( Young Jin Lee ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구는 백두대간에 위치하고 있는 오대산 국립공원 소나무림을 대상으로 2012년 6월부터 2013년 5월까지 지상부와 지하부 현존량, 낙엽생산량, 낙엽층의 낙엽량과 토양의 유기탄소 분포를 조사하였으며, 탄소수지를 파악하기 위하여 토양호흡을 측정하였다. 오대산 소나무림에서 조사된 총 유기탄소량은 230.11 ton C/ha으로, 지상부와 지하부의 유기탄소량은 각각 182.78 ton C/ha, 45.70 tonC/ha이었으며, 낙엽층과 토양의 유기탄소량은 각각 5.98 ton C/ha, 45.40 ton C/ha이었다. 오대산 소 나무림에서 연간 광합성을 통하여 식물체에 고정되는 유기탄소량은 17.76 ton C/ha/yr로 임내 층위에 따라 유기탄소가 고정되어 있다. 또한 조사기간 동안 낙엽을 통하여 임상으로 유입되는 유기탄소 량은 3.233 ton C/ha/yr이었다. 토양호흡을 통하여 방출되는 탄소량은 5.76 ton C/ha/yr으로 이 중미생물호흡을 통해 방출되는 탄소량은 3.11 ton C/ha/yr이고, 뿌리호흡을 통하여 방출되는 탄소량은2.65 ton C/ha/yr이었다. 그 결과, 오대산 소나무림에서 연간 대기로부터 순 흡수하는 유기탄소는 14.65 ton C/ha/yr로 조사되었다. This study was conducted to estimate carbon sequestration and carbon budget of Pinus densiflora stands in the Mt. Odae National Park. Above and below ground standing biomass, litter layer, soil organic carbon, and soil respiration were measured to estimate the amount of carbon and the carbon budget from June 2012 through May 2013. Total amount of carbon was 230.11 ton C/ha attributed to above (182.78 ton C/ha) and below (45.70 ton C/ha) ground biomass, litter layer (5.98 ton C/ha) and soil organic carbon (45.40 ton C/ha). This was equivalent to 17.76 ton C/ha/yr from above and below ground biomass, shrub and herb. In addition, the amount of organic carbon returned to the forest through litterfall was 3.233 ton C/ha/yr. The amount of carbon evolved through soil respiration was 5.76 ton C/ha/yr to which 3.11 ton C/ha/yr was accounted for microbial respiration while the remaining 2.65 ton C/ha/yr was due to root respiration. Based on the estimation of the difference between Net Primary Production and microbial respiration, the amount of atmospheric carbon sequestered by Pinus densiflora stands was 14.65 ton C/ha/yr.

오대산 신갈나무림의 낙엽생산과 유기탄소 동태

장미나 ( Mi Na Jang ),김성용 ( Sung Yong Kim ),( Azyleah C. Abino ),김창현 ( Chang Hyun Kim ),이중효 ( Jung Hyo Lee ),이영진 ( Young Jin Lee ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구는 백두대간에 위치하고 있는 오대산 국립공원 신갈나무림을 대상으로 2012년 6월부터 2013년 5월까지 지상부와 지하부 현존량, 낙엽생산량, 낙엽층의 낙엽량과 토양의 유기탄소 분포를 조사하였으며, 탄소수지를 파악하기 위하여 토양호흡을 측정하였다. 오대산 신갈나무림에서 조사된 총유기탄소량은 93.07 ton C/ha으로, 지상부와 지하부의 유기탄소량은 각각 72.36 ton C/ha, 18.09 tonC/ha이었으며, 낙엽층과 토양의 유기탄소량은 각각 4.39 ton C/ha, 42.28 ton C/ha이었다. 오대산 신갈나무림에서 연간 광합성을 통하여 식물체에 고정되는 유기탄소량은 5.46 ton C/ha/yr로 임내 층위에 따라 교목층 4.26 ton C/ha/yr, 관목층 0.24 ton C/ha/yr, 초본층 0.95 ton C/ha/yr의 유기탄소가 고정되어 있다. 또한 조사기간 동안 낙엽을 통하여 임상으로 유입되는 유기탄소량은 2.840 tonC/ha/yr이었다. 토양호흡을 통하여 방출되는 탄소량은 5.52 ton C/ha/yr으로 이 중 미생물호흡을 통해 방출되는 탄소량은 2.98 ton C/ha/yr이고, 뿌리호흡을 통하여 방출되는 탄소량은 2.54 tonC/ha/yr이었다. 그 결과, 오대산 신갈나무림에서 연간 대기로부터 순 흡수하는 유기탄소는 2.48 tonC/ha/yr로 조사되었다. This study was conducted to estimate Litter Production and carbon budget of Quercus mongolica stands in the Mt. Odae National Park. Above and below ground standing biomass, litter layer, soil organic carbon, and soil respiration were measured to estimate the amount of carbon and the carbon budget from June 2012 through May 2013. Total amount of carbon was 93.07 ton C/ha attributed to above (72.36 ton C/ha) and below (18.09 ton C/ha) ground biomass, litter layer (4.39 ton C/ha) and soil organic carbon (42.28 ton C/ha). This was equivalent to 5.46 ton C/ha/yr where 4.26 ton C/ha/yr is from above and below ground biomass, 0.24 ton C/ha/yr from shrub and 0.95 ton C/ha/yr from herb. In addition, the amount of organic carbon returned to the forest through litterfall was 2.840 ton C/ha/yr. The amount of carbon evolved through soil respiration was 5.52 ton C/ha/yr to which 2.98 ton C/ha/yr was accounted for microbial respiration while the remaining 2.54 ton C/ha/yr was due to root respiration. Based on the estimation of the difference between Net Primary Production and microbial respiration, the amount of atmospheric carbon sequestered by Quercus mongolica stands was 2.48 ton C/ha/yr.

Poster Electrical properties of SrRuO₃ thin films with varying c-axis lattice constant

Young J. Chang,C. U. Jung 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Electrical Properties of SrRuO₃ Thin Films with Varying c-axis Lattice Constant

Young J. Chang,Jin I Kim,C. U. Jung 한국자기학회 2008 Journal of Magnetics Vol.13 No.2

We studied the effect of the variation of the lattice constant on the electrical properties of SrRuO₃thin films. In order to obtain films with different volumes, we varied the substrate temperature and oxygen pressure during the growth of the films on SrTiO₃(001) substrates. The films were grown using a pulsed laser deposition method. The X-ray diffraction patterns of the grown films at low temperature and low oxygen pressure indicated the elongation of the c-axis lattice constant compared to that of the films grown at a higher temperature and higher oxygen pressure. The in-plane strain states are maintained for all of the films, implying the expansion of the unit-cell volume by the oxygen vacancies. The variation of the electrical resistance reflects the temperature dependence of the resistivity of the metal, with a ferromagnetic transition temperature inferred form the cusp of the curve being observed in the range from 110 K to 150 K. As the c-axis lattice constant decreases, the transition temperature linearly increases.

Evaluation of Neuronal Dysfunction in Schizophrenia before and after Neuroleptic Treatment by ??H MRS

C, Bo-Young,Paik, In-Ho,Lee, Chang-Uk,Lee, Hyoung-Koo,Suh, Tae-Suk Korean Magnetic Resonance Society 2001 Journal of the Korean Magnetic Resonance Society Vol.5 No.1

Localized in vivo proton magnetic resonance spectroscopy (MRS) was performed to evaluate metabolic alterations in the right and left frontal lobe before and after neuroleptic treatment of schizophrenic patients (n=24) and a group of healthy normal subjects (n=20). Proton metabolic ratios obtained from the 8㎤ yokels in the right and left frontal lobe were compared with the clinical assessment of PANSS for each subject. There was no significant difference in the metabolic ratios between the right and the left frontal lobes in either the schizophrenic group or the control group, indicating no laterality. Compared with those of the normal control group, NAA/Cr and (GABA+Glu)/Cr ratios of the schizophrenic patients showed significantly lower (p=0.023) and higher (p=0.005) value, respectively. The (GABA+Glu)/Cr ratio of the schizophrenic patients was generally decreased after neuroleptic treatment, while the NAA/Cr ratio was not changed. Significant correlation between the (GABA+Glu)/Cr ratio and the clinical symptom scores assessed by PANSS was established. The present study supports the “hypofrontality” hypothesis of schizophrenia on the basis of the altered metabolic ratios before and after neuroleptic treatment.

Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor

Chang, Young-C.,Park, Chan-Koo,Jung, Kweon,Kikuchi, Shintaro Korean Society of Environmental Health 2010 한국환경보건학회지 Vol.36 No.4

We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE) removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2-dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at $0.006\;mg\;protein^{-1}{\cdot}l{\cdot}h^{-1}$, and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from $30\;{\mu}M$ to $905\;{\mu}M$, the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be $1,100\;{\mu}mol{\cdot}d^{-1}l$ of reactor $volume^{-1}$, which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.

Dechlorination of High Concentrations of Tetrachloroethylene Using a Fixed-bed Reactor

Young C. Chang,Chan-Koo Park,Kweon Jung,Shintaro Kikuchi 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.4

We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE) removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2- dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at 0.006 mg protein−1 · l · h−1, and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from 30 μM to 905 μM, the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be 1,100 μmol · d−1l of reactor volume−1, which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.

영국의 비파괴검사 기술자 자격인정 및 인증 체계

장영권,이종포,하기수,한치현 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

A New Species of the Genus Copidognathus (Halacaridae: Acari) from India

Tapas Chatterjee,C. Annapurna,Cheon Young Chang 한국통합생물학회 2003 Animal cells and systems Vol.7 No.4

Copidognathus bengalensis n. sp. is described from Visakhapatnam coast, Bay of Bengal, India. The present species is characterized by posterodorsal plate (PD) with four costae made up of porose panels, subdivided posterior cornea of ocular plate (OC), and two pairs of basirostral setae in female. This species is related to C. pulcher group, but the nature of porose panels on anterior areolae of anterior dorsal plate (AD) and the setal ornamentation of legs were different between the members of C. pulcher group and the present new species.