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두경부 편평상피세포암 세포주의 염색체 이상 분석:비교유전체보합법과 Array 비교유전체보합법
신유리(You Ree Shin),박수연(Soo-Yeun Park),이동욱(Dong Wook Lee),김한수(Han Su Kim),고영민(Young Min Go),박현주(Hyun Joo Park),정성민(Sung Min Choung) 대한두경부종양학회 2008 대한두경부 종양학회지 Vol.24 No.1
Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15-p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more de-tailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.
You Ree Jung,Hyun Yong Shin,송윤석,Sung Bong Kim,김승욱 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2
In this study, b-glucosidase from Aspergillus niger was pretreated with cellobiose and glucose to prevent loss of enzyme activity, and pretreated b-glucosidase was immobilized on silica gel as a carrier by covalent binding. To enhance the activity of immobilized b-glucosidase, the effects of substrate concentration and reaction conditions, including temperature, time, and agitation speed, were investigated. The optimal concentrations of cellobiose and glucose, temperature, time, and agitation speed were determined to be 0.02 M, 40 8C, 20 min, and 130 rpm, respectively. The activity of immobilized b-glucosidase after pretreatment was increased to about 176% of that of non-pretreated bglucosidase. In addition, the optimal pH and temperature of the non-pretreated and pretreated immobilized b-glucosidases were both pH 5.5 and 65 8C, respectively. Moreover, the immobilized bglucosidases were used repeatedly 20 times, and the enzyme activities were maintained at levels higher than 80% of their initial activities.
Production of cellulases and β-glucosidase in Trichoderma reesei mutated by proton beam irradiation
You Ree Jung,김승욱,Hyun Yong Shin,Hah Young Yoo,엄영순 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.7
To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and β-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T.reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel’s fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of β-glucosidase, compared with the wild type strain. Additionally, the properties of high level β-glucosidase produced by MT-2 were the same as those of the wild type strain, e.g., an optimum pH of 4.8, and an optimum temperature of 65 oC. Moreover, the protein concentrations of β-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then β-glucosidase activities were detected by the MUG-zymogram assay.
Jung, You Ree,Shin, Hyun Yong,Song, Yoon Seok,Kim, Sung Bong,Kim, Seung Wook Elsevier 2012 Journal of industrial and engineering chemistry Vol.18 No.2
<P><B>Abstract</B></P><P>In this study, <I>β</I>-glucosidase from <I>Aspergillus niger</I> was pretreated with cellobiose and glucose to prevent loss of enzyme activity, and pretreated <I>β</I>-glucosidase was immobilized on silica gel as a carrier by covalent binding. To enhance the activity of immobilized <I>β</I>-glucosidase, the effects of substrate concentration and reaction conditions, including temperature, time, and agitation speed, were investigated. The optimal concentrations of cellobiose and glucose, temperature, time, and agitation speed were determined to be 0.02M, 40°C, 20min, and 130rpm, respectively. The activity of immobilized <I>β</I>-glucosidase after pretreatment was increased to about 176% of that of non-pretreated <I>β</I>-glucosidase. In addition, the optimal pH and temperature of the non-pretreated and pretreated immobilized <I>β</I>-glucosidases were both pH 5.5 and 65°C, respectively. Moreover, the immobilized <I>β</I>-glucosidases were used repeatedly 20 times, and the enzyme activities were maintained at levels higher than 80% of their initial activities.</P>
Son A Chang,Sujin Shin,Sungkeong Kim,Yeabitna Lee,Eun Young Lee,Hanee Kim,You-Ree Shin,Young-Myoung Chun 한국음성학회 2021 말소리와 음성과학 Vol.13 No.2
In this study, we investigated longitudinal music perception of adult cochlear implant (CI) users and how acoustic stimulation with CI affects their music performance. A total of 163 participants" data were analyzed retrospectively. 96 participants were using acoustic stimulation with CI and 67 participants were using electrical stimulation only via CI. The music performance (melody identification, appreciation, and satisfaction) data were collected pre-implantation, 1-year, and 2-year post-implantation. Mixed repeated measures of ANOVA and pairwise analysis adjusted by Tukey were used for the statistics. As result, in both groups, there were significant improvements in melody identification, music appreciation, and music satisfaction at 1-year, and 2-year post-implantation than a pre-implantation, but there was no significant difference between 1 and 2 years in any of the variables. Also, the group of acoustic stimulation with CI showed better perception skill of melody identification than the CI-only group. However, no differences found in music appreciation and satisfaction between the two groups, and possible explanations were discussed. In conclusion, acoustic and/or electrical hearing devices benefit the recipients in music performance over time. Although acoustic stimulation accompanied with electrical stimulation could benefit the recipients in terms of listening skills, those benefits may not extend to the subjective acceptance of music. These results suggest the need for improved sound processing mechanisms and music rehabilitation.
Conductive deafness with normal eardrum: absence of the long process of the incus
Park, Keehyun,Choung, Yun-Hoon,Shin, You Ree,Hong, Sung Pyo Scandinavian University Press 2007 Acta oto-laryngologica Vol.127 No.8
<P> Conclusions. The most likely cause of lesions with an absence of the long process of the incus was congenital origin because many cases were associated with the stapedial anomaly. However, the possibility of a potential inflammation of this lesion could not be completely excluded, especially in cases with only an absence of the long process of the incus. Objective. To clarify whether the absence of the long process of the incus is caused by congenital anomaly or a potential inflammation. Subjects and methods. A total of 21 patients (5 bilateral cases, 16 unilateral cases) with absence of the long process of the incus were reviewed. Operative and histopathological findings of the removed incus were analyzed. Results. The 21 cases with absence of the long process of the incus were classified into 6 types. Thirteen cases showed the absence of the long process associated with the stapedial anomaly, which were all unilateral. Eight cases showed no association with the stapedial anomaly, among which five cases were bilateral. Three of eight cases not associated with the stapedial anomaly showed evidence of resorption in the incus body such as fibrosis and Haversian canal widening.</P>
Visualization of the melanosome transfer-inhibition in a mouse epidermal cell co-culture model.
Kim, Hae Jong,Kazi, Julhash U,Lee, You-Ree,Nguyen, Dung H,Lee, Hyang-Bok,Shin, Jeong-Hyun,Soh, Jae-Won,Kim, Eun-Ki D.A. Spandidos 2010 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.25 No.2
<P>Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the melanosome, by using green fluorescent protein and red fluorescent protein. The intracellular localization of tyrosinase was then examined by fluorescence and confocal microscopy. Co-culture of murine melanocytes and keratinocytes was optimized and melanosome transfer was either stimulated with alphaMSH or partially inhibited by niacinamide. To the best of our knowledge, this is the first study showing that a murine co-culture model, in addition to human primary cell co-culture, can be a good tool for depigmenting agent screening by monitoring melanosome transfer.</P>
Yang-soo Yoon,Aiguo Liu,You-Ree Shin,Jae-sook Gho,Candace Hicks,Qian-Jie Fu,Allison Coltisor 한국언어재활사협회 2016 Clinical Archives of Communication Disorders Vol.1 No.1
Purpose: The present study used the acoustic simulation of bilateral cochlear implants to investigate the effect of presentation level (EXP 1) and channel interaction (EXP 2) on binaural summation benefit in speech recognition. Methods: The acoustic 6-channel processors were constructed, and the envelope of each band was used to modulate a sinusoid (EXP 1) or band-pass filtered white noise (EXP 2) with a frequency matching the center frequency of the carrier band. Presentation level was fixed for one ear and varied for the other. Channel interaction was simulated by altering filter slopes while keeping interaural spectral peaks matched in the carrier bands. Two separate groups of ten adult subjects with normal hearing participated in EXP 1 and EXP 2. Sentence recognition was measured with left ear alone, right ear alone, and both ears in quiet and in noise at +5 dB signal-to-noise ratio. Results: A significant binaural summation benefit occurs only in noise, regardless of interaural mismatch in channel interactions and presentation levels. Conclusions: Results suggest that factors other than channel interaction are important and that matching interaural loudness is an unimportant factor in binaural summation benefit in noise. For both EXP 1 and EXP 2, the data trend is indicative of speech information perceived in quiet being fully coded by the better performing ear alone, which leads to no binaural summation benefit. The results of current and future studies could have implications in programming of bilateral cochlear implant users.