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Yoo, J,Lee, S,Jung, Y,Lee, J,Youm, D,Ha, H,Kim, H,Ko, R-K,Oh, S Institute of Physics 2008 Journal of physics. Conference series Vol.97 No.1
<P>We measured the field profiles, <I>H</I>(<I>x,H</I><SUB>a</SUB>) s, near the surface of coated conductors (CCs) by using the scanning Hall probe method. The samples were SmBCO-CC tape fabricated by co-evaporation method and YBCO-CC tape fabricated by PLD method. The applied fields, <I>H</I><SUB>a</SUB>s, were decreased from <I>H</I><SUB>peak</SUB>to -<I>H</I><SUB>peak</SUB> stepwise. From the values of <I>H</I>(<I>x,H</I><SUB>a</SUB>), we calculated the current profiles, <I>J</I>(<I>x,H</I><SUB>a</SUB>) s, by the inversion method. From the values of <I>J</I>(<I>x,H</I><SUB>a</SUB>) and the corresponding flux densities, we calculated the hysteretic energy losses per cycle, <I>Q</I><SUB>M</SUB>s, for various <I>H</I><SUB>peak</SUB>s. From the values of <I>Q</I><SUB>M</SUB>, we calculated the characteristic functions, <I>g</I>s, by using the relation, <I>g</I>= π<I>Q</I><SUB>M</SUB>/μ<SUB>0</SUB><I>I</I><SUP>2</SUP><SUB>c</SUB>. Here, <I>I</I><SUB>c</SUB> is the critical current. For the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB>≤ 3, the <I>g</I>-values of SmBCO CC tape were larger than those of YBCO CC tape. However, for the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> ≥ 3, the <I>g</I>-values of SmBCO CC tape were smaller than those of YBCO CC tape. When <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> = 3, both sample show almost same value of <I>g.</I>However we found qualitatively different <I>J–B</I> hysteretic curves for both samples. We also compared our <I>g</I>-values with other <I>g</I>-values, which were directly measured by energy loss experiments. Our <I>g</I>-values of YBCO CC tapes were basically similar to the Brandt's theoretical values of <I>g</I> in the most range of <I>I</I><SUB>peak</SUB> in our measurements.</P>
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과산화수소가 함유된 저산도 질산용액에서 DEHPA 추출제에 의한 Np 의 추출거동
이일희,양한범,김광욱,임재관,유재형 ( E . H . Lee,H . B . Yang,K . K . Kim,J . K . Lim,J . H . Yoo ) 한국공업화학회 1996 공업화학 Vol.7 No.4
본 연구는 Np의 환원제로 H₂O₂가 함유된 IM 이하의 저산도 질산용액으로부터 DEHPA(di-(2-ethyhexyl)phosphoric acid) 추출제에 의한 Np의 추출 및 역추출 조건 설정과 추출속도 향상에 주안점을 두어, 회분식으로 실험을 수행하였다. 저산도 질산용액에서 Np의 산화상태는 주로 Np(V)로 존재하고 있음을 확인하였으며, NP의 추출율은 H₂0₂농도 및 DEHPA의 농도 증가에 따라 증가하고, 질산농도 증가에 따라 급격히 감소하였다. 제3의 산화/환원제가 첨가되지 않는 경우 추출율은 약 70% 정도로 다소 낮지만, DEHPA에 의해 추출이 가능함을 보았다. 또한 추출속도는 H₂O₂농도의 0.516 승에 비례하며, 질산농도의 0.483 승에 반비례하고 있는 다음과 같은 식을 얻었다. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] 그리고 과산화수소의 첨가 유무에 관계없이, 유기상으로 추출된 Np은 옥살산(oxalic acid)에 의해 효과적으로 역추출되었으며, 0.5M 옥살산으로 약 92% 이상을 역추출하였다. Extraction behaviour of Np with DEHPA(di-(2-ethyhexyl) phosphoric acid) from the low nitric acid solution(below 1M HNO₃) containing H₂O₂as a reducing agent was studied at a batch system in order to establish the conditions of extraction and stripping and to enhance the extraction rate. As results, it was confirmed that the Np was mainly the pentavalent oxidation state in the low nitric acid solution. The extraction yield of Np was increased with increasing the concentration of DEHPA and H₂O₂and decreased more rapidly with the increase of HNO₃ concentration. It was also found that the Np could be extracted into DEHPA even without the addition any redox agents, although the extraction yield is rather low as about 70%. The extraction rate was proportional to the 0.516 power of H₂O₂concentration and inversely proportional to 0.483 power of HNO, concentration as follows. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] Regardless of the H₂O₂, the Np extracted in the organic phase was effectively stripped to the aqueous phase with H₂C₂O₄. The Np could be stripped more than 92% with 0.5M H₂C₂O₄.
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Chae, H.S.,Singh, N.K.,Ahn, C.N.,Yoo, Y.M.,Jeong, S.G.,Ham, J.S.,Kim, D.W. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11
The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce anti-E. coli O157:H7 antibodies. The immune response and the egg yolk antibodies of laying hens against the whole cell, outer membrane protein and LPS antigens were monitored by ELISA. The level of antibodies against whole cell antigen monitored through ELISA sharply increased after the initial immunization, and it was found to be maximum on day 49 however, the level was maintained up to day 70. Antibodies (5 mg/ml) directed against the whole cell inhibited E. coli proliferation 10-13 times more than outer membrane protein or LPS. The antibody response against the whole cell antigens appeared to have higher activity in restraining the proliferation of E. coli O157:H7 than antibody against outer membrane protein or LPS. Results reflected that increasing the IgY's in the egg yolk could prevent greater economic losses due to human and animal health from pathogenic bacteria i.e. E. coli O157:H7.
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Kim, H.,Yoo, J.E.,Cho, J.Y.,Oh, B.K.,Yoon, Y.S.,Han, H.S.,Lee, H.S.,Jang, J.J.,Jeong, S.H.,Kim, J.W.,Park, Y.N. Elsevier Science Publishers 2013 Journal of hepatology Vol.59 No.4
Background & Aims: Hepatocellular carcinomas (HCCs) expressing ''stemness''-related markers have been associated with aggressive biological behavior and poor prognosis. We examined the relationship between ''stemness''-related protein expression and telomere length, hTERT and shelterin complex protein expression and chromosomal instability. Methods: Quantitative fluorescent in situ hybridization for telomere length, immunohistochemistry for K19, EpCAM, CD133, c-kit, HepPar1, hTERT, TRF1, TRF2, POT1, RAP1 and TPP1, and TUNEL assay were performed in 137 HCCs, and array comparative genomic hybridization was performed with 24 HCCs. Results: Telomeres were significantly longer in HCCs expressing ''stemness''-related proteins (K19: p<0.001, EpCAM: p=0.002, CD133: p=0.002). On analyzing different tumor cells within EpCAM-expressing HCCs, EpCAM-positive tumor cells showed longer telomeres (1.329+/-0.246) compared to EpCAM-negative tumor cells (0.996+/-0.381) within the same HCCs (p=0.031). Telomeres were significantly longer in HCCs expressing hTERT (p=0.048) and RAP1 proteins (p=0.031). K19-expressing HCCs expressed hTERT (p=0.002), TRF2 (p=0.001) and TPP1 (p=0.013) more frequently compared to K19-negative HCCs. EpCAM-positivity was associated with more frequent hTERT (p=0.028), TPP1 (p=0.017), TRF2 (p=0.027) and POT1 (p=0.004) expression. Copy number alterations were more frequent in K19 and EpCAM-expressing HCCs compared to HCCs without these markers (K19: p=0.038, EpCAM: p=0.009). HCCs with longer telomeres were associated with a shorter overall (p=0.019) and disease-free survivals (p=0.049), and decreased disease-free survivals were seen in TRF2-positive HCCs (p=0.018). Conclusions: HCCs expressing ''stemness''-related proteins are characterized by increased telomere length, increased expression of hTERT and shelterin complex proteins, and increased chromosomal instability compared to conventional HCCs. Longer telomeres and TRF2 expression in HCCs are associated with poor patient outcomes.
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L H Hwang,J H Yoo,H S Song,S K Na,H S Kim,H S Oh,S H Lee,K H Choi,M T Cho 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-
Recently, 35W class Fluorescent lamps with 32㎜ tube diameter are replaced with 32W one with 26 ㎜ in diameter to conserve lamp materials and to increase luminance efficiency. Moreover, 35, 28 and 14 W fluorescent lamps with 16㎜ (T5) in diameter, which are nowadays developed, also may replace 32 W lamps again. Application of slim lamps, however, requires small sized electronic ballast to full fill the design philosophy of miniaturizing. However, the traditional magnetic ballasts operated at 50-60㎐ have been suffered from noticeable flicker, high loss, large crest factor and heavy weight. In this study, in order to solve these problems, a new type of electronic ballast, which is composed of rectifier, active power factor corrector, series resonant half bridge inverter and piezoelectric transformer, was proposed for driving T5 fluorescent lamp. Driving of piezoelectric transformer was carried out with input region for the ring electrode and output region for the dot electrode. A 35W (T5) fluorescent lamp is successfully driven by the fabricated ballast with piezoelectric transformer. After driving the lamp using the proposed electronic ballast for 20 min, the input power factor and efficiency of ballast shown 0.95 and 86%, respectively, at operating frequency of 81 k㎐. And also, the output power and temperature rise of piezoelectric transformer showed 35.07W and 20.5 , respectively.
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In vivo imaging of tumor apoptosis using histone H1-targeting peptide
Wang, K.,Purushotham, S.,Lee, J.Y.,Na, M.H.,Park, H.,Oh, S.J.,Park, R.W.,Park, J.Y.,Lee, E.,Cho, B.C.,Song, M.N.,Baek, M.C.,Kwak, W.,Yoo, J.,Hoffman, A.S.,Oh, Y.K.,Kim, I.S.,Lee, B.H. Elsevier Science Publishers 2010 Journal of controlled release Vol.148 No.3
In vivo imaging of apoptosis could allow monitoring of tumor response to cancer treatments such as chemotherapy. Using phage display, we identified the CQRPPR peptide, named ApoPep-1(Apoptosis-targeting Peptide-1), that was able to home to apoptotic and necrotic cells in tumor tissue. ApoPep-1 also bound to apoptotic and necrotic cells in culture, while only little binding to live cells was observed. Its binding to apoptotic cells was not dependent on calcium ion and not competed by annexin V. The receptor for ApoPep-1 was identified to be histone H1 that was exposed on the surface of apoptotic cells. In necrotic cells, ApoPep-1 entered the cells and bound to histone H1 in the nucleus. The imaging signals produced during monitoring of tumor apoptosis in response to chemotherapy was enhanced by the homing of a fluorescent dye- or radioisotope-labeled ApoPep-1 to tumor treated with anti-cancer drugs, whereas its uptake of the liver and lung was minimal. These results suggest that ApoPep-1 holds great promise as a probe for in vivo imaging of apoptosis, while histone H1 is a unique molecular signature for this purpose.
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Overview of KSTAR research progress and future plans toward ITER and K-DEMO
Park, H.K.,Choi, M.J.,Hong, S.H.,In, Y.,Jeon, Y.M.,Ko, J.S.,Ko, W.H.,Kwak, J.G.,Kwon, J.M.,Lee, J.,Lee, J.H.,Lee, W.,Nam, Y.B.,Oh, Y.K.,Park, B.H.,Park, J.K.,Park, Y.S.,Wang, S.J.,Yoo, M.,Yoon, S.W.,B IOP 2019 Nuclear fusion Vol.59 No.11
<P>A decade-long operation of the Korean Superconducting Tokamak Advanced Research (KSTAR) has contributed significantly to the operation of superconducting tokamak devices and the advancement of tokamak physics which will be beneficial for the ITER and K-DEMO programs. Even with limited heating capability, various conventional as well as new operating regimes have been explored and have achieved improved performance. As examples, a long pulse high-confinement mode operation with and without an edge-localized mode (ELM) crash was well over 70 and 30 s, respectively. The unique capabilities of KSTAR allowed it to improve the capability of controlling harmful instabilities, and they have been instrumental in uncovering much new physics. The highlights are that the L/H transition threshold power is sensitive to the resonant magnetic perturbation (RMP) and insensitive to non-resonant magnetic perturbation. Co-<I>I</I> <SUB>p</SUB> offset rotation dominated by an electron channel predicted by general neoclassical toroidal viscosity theory was confirmed. Improved heat dispersal in a divertor system using three rows of rotating RMP was demonstrated and predictive control of the ELM-crash with <I>a priori</I> modeling was successfully tested. In magnetohydrodynamic physics, validation of the full reconnection model (i.e. <I>q</I> <SUB>0</SUB> > 1 right after the sawtooth crash) and self-consistent validation of the anisotropic distribution of turbulence amplitude and flow in the presence of the 2/1 island with theoretical models were achieved. The turbulence amplitude induced by RMP was linearly increased with the slow RMP coil current ramp-up time (i.e. the magnetic diffusion time scale). The <I>D</I> <SUB> <I>α</I> </SUB> spikes (i.e. ELM-crash amplitude) was linearly decreased with the turbulence amplitude and not correlated with the perpendicular electron flow. In the turbulence area, a non-diffusive ‘avalanche’ transport event and the role of a quiescent coherent mode in confinement were studied. To accommodate the anticipation of a higher performance of the KSTAR plasmas with the increased heating powers, a new divertor/internal interface with a full active cooling system will be implemented after a full test of the new heating (neutral beam injection II and electron cyclotron heating) and current drive (CD) (Helicon and lower hybrid CD) systems. An upgrade plan for the internal hardware, heating systems and efficient CD system may allow for a long pulse operation of higher performance plasmas at <I>β</I> <SUB>N</SUB> > 3.0 with <I>f</I> <SUB>bs</SUB> ~ 0.5 and <I>T</I> <SUB>i</SUB> > 10 keV.</P>
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Novel dentin phosphoprotein frameshift mutations in dentinogenesis imperfecta type II
Lee, K‐,E,Kang, H‐,Y,Lee, S‐,K,Yoo, S‐,H,Lee, J‐,C,Hwang, Y‐,H,Nam, KH,Kim, J‐,S,Park, J‐,C,Kim, J‐,W Blackwell Publishing Ltd 2011 Clinical genetics Vol.79 No.4
<P>Lee K‐E, Kang H‐Y, Lee S‐K, Yoo S‐H, Lee J‐C, Hwang Y‐H, Nam KH, Kim J‐S, Park J‐C, Kim J‐W. Novel dentin phosphoprotein frameshift mutations in dentinogenesis imperfecta type II.</P><P>The dentin sialophosphoprotein (<I>DSPP</I>) gene encodes the most abundant non‐collagenous protein in tooth dentin and DSPP protein is cleaved into several segments including the highly phosphorylated dentin phosphoprotein (DPP). Mutations in the <I>DSPP</I> gene have been solely related to non‐syndromic form of hereditary dentin defects. We recruited three Korean families with dentinogenesis imperfecta (DGI) type II and sequenced the exons and exon–intron boundaries of the <I>DSPP</I> gene based on the candidate gene approach. Direct sequencing of PCR products and allele‐specific cloning of the highly repetitive exon 5 revealed novel single base pair (bp) deletional mutations (c.2688delT and c.3560delG) introducing hydrophobic amino acids in the hydrophilic repeat domain of the DPP coding region. All affected members of the three families showed exceptionally rapid pulp chambers obliteration, even before tooth eruption. Individuals with the c.3560delG mutation showed only mild, yellowish tooth discoloration, in contrast to the affected individuals from two families with c.2688delT mutation. We believe that these results will help us to understand the molecular pathogenesis of DGI type II as well as the normal process of dentin biomineralization.</P>
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