Use of Compressive Sensing Technique for Spatial Interpolation of Gauge Rainfall Rate

Soorok Ryu,Yongku Kim,Sung-Hwa Jung,Yonghae Do,GyuWon Lee 한국기상학회 2015 한국기상학회 학술대회 논문집 Vol.2015 No.10

Colorectal Cancer Therapy Using a Pediococcus pentosaceus SL4 Drug Delivery System Secreting Lactic Acid Bacteria-Derived Protein p8

정명준,안병철,Yongku Ryu,Yeo-Sang Yoon,Oksik Choi,박호진,김태엽,김송인,김봉규 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Despite decades of research into colorectal cancer (CRC), there is an ongoing need for treatments that are more effective and safer than those currently available. Lactic acid bacteria (LAB) show beneficial effects in the context of several diseases, including CRC, and are generally regarded as safe. Here, we isolated a Lactobacillus rhamnosus (LR)-derived therapeutic protein, p8, which suppressed CRC proliferation. We found that p8 translocated specifically to the cytosol of DLD-1 cells. Moreover, p8 down-regulated expression of Cyclin B1 and Cdk1, both of which are required for cell cycle progression. We confirmed that p8 exerted strong anti-proliferative activity in a mouse CRC xenograft model. Intraperitoneal injection of recombinant p8 (r-p8) led to a significant reduction (up to 59%) in tumor mass when compared with controls. In recent years, bacterial drug delivery systems (DDSs) have proven to be effective therapeutic agents for acute colitis. Therefore, we aimed to use such systems, particularly LAB, to generate the valuable therapeutic proteins to treat CRC. To this end, we developed a gene expression cassette capable of inducing secretion of large amounts of p8 protein from Pediococcus pentosaceus SL4 (PP). We then confirmed that this protein (PP-p8) exerted anti-proliferative activity in a mouse CRC xenograft model. Oral administration of PP-p8 DDS led to a marked reduction in tumor mass (up to 64%) compared with controls. The PP-p8 DDS using LAB described herein has advantages over other therapeutics; these advantages include improved safety (the protein is a probiotic), cost-free purification, and specific targeting of CRC cells.

Nonparametric Bayesian modeling for monotonicity in catch ratio

Kim, Dal Ho,Ryu, Hyunnam,Kim, Yongku Informa UK (TaylorFrancis) 2018 Communications in statistics. Simulation and compu Vol.47 No.4

<P>This article proposes nonparametric Bayesian approaches to monotone function estimation. This approach uses a hierarchical Bayes framework and a characterization of stick-breaking process that allows unconstrained estimation of the monotone function. In order to avoid the limitation of parametric modeling, a general class of prior distributions, called stick-breaking priors, is considered. It accommodates much more flexible forms and can easily deal with skewness, multimodality, etc., of the dependent variable response. The proposed approach is incorporated to model the catch ratio based on automatic weather station (AWS) data.</P>

Colorectal Cancer Therapy Using a Pediococcus pentosaceus SL4 Drug Delivery System Secreting Lactic Acid Bacteria-Derived Protein p8

An, Byung Chull,Ryu, Yongku,Yoon, Yeo-Sang,Choi, Oksik,Park, Ho Jin,Kim, Tai Yeub,Kim, Song-In,Kim, Bong-Kyu,Chung, Myung Jun Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.11

Despite decades of research into colorectal cancer (CRC), there is an ongoing need for treatments that are more effective and safer than those currently available. Lactic acid bacteria (LAB) show beneficial effects in the context of several diseases, including CRC, and are generally regarded as safe. Here, we isolated a Lactobacillus rhamnosus (LR)-derived therapeutic protein, p8, which suppressed CRC proliferation. We found that p8 translocated specifically to the cytosol of DLD-1 cells. Moreover, p8 down-regulated expression of Cyclin B1 and Cdk1, both of which are required for cell cycle progression. We confirmed that p8 exerted strong anti-proliferative activity in a mouse CRC xenograft model. Intraperitoneal injection of recombinant p8 (r-p8) led to a significant reduction (up to 59%) in tumor mass when compared with controls. In recent years, bacterial drug delivery systems (DDSs) have proven to be effective therapeutic agents for acute colitis. Therefore, we aimed to use such systems, particularly LAB, to generate the valuable therapeutic proteins to treat CRC. To this end, we developed a gene expression cassette capable of inducing secretion of large amounts of p8 protein from Pediococcus pentosaceus SL4 (PP). We then confirmed that this protein (PP-p8) exerted anti-proliferative activity in a mouse CRC xenograft model. Oral administration of PP-p8 DDS led to a marked reduction in tumor mass (up to 64%) compared with controls. The PP-p8 DDS using LAB described herein has advantages over other therapeutics; these advantages include improved safety (the protein is a probiotic), cost-free purification, and specific targeting of CRC cells.

Electrochemical performance of all-solid lithium ion batteries with a polyaniline film cathode

Oh, Ji-Woo,Oh, Rye-Gyeong,Kang, Yongku,Ryu, Kwang-Sun Elsevier 2016 Journal of Energy Chemistry Vol.25 No.1

<P>We have prepared a high-density polyaniline (PANI) paste (50 mg/mL), with similar physical properties to those of paints or pigments. The synthesis of PANI is confirmed by Fourier transform infrared (FT-IR) spectroscopy. The morphologies of PANI, doped PANI, and doped PANI paste are confirmed by scanning electron microscopy (SEM). Particles of doped PANI paste are approximately 40-50 nm in diameter, with a uniform and cubic shape. The electrochemical performances of doped PANI paste using both liquid and solid polymer electrolytes have been measured by galvanostatic charge and discharge process. The cell fabricated with doped PANI paste and the solid polymer electrolyte exhibits a discharge capacity of similar to 87 mu Ah/cm(2) (64.0 mAh/g) at the second cycle and 67 mu Ah/cm(2) (50.1 mAh/g) at the 100th cycle. (C) 2015 Science Press and Dalian Institute of Chemical Physics. All rights reserved.</P>

Lactobacillus rhamnosus CBT-LR5 Improves Lipid Metabolism by Enhancing Vitamin Absorption

Kim Dong-Jin,Kim Tai Yeub,Yoon Yeo-Sang,Ryu Yongku,Chung Myung Jun 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.4

Probiotics provide a symbiotic relationship and beneficial effects by balancing the human intestinal microbiota. The relationships between microbiota changes and various diseases may predict health abnormalities and diseases. Treatment with vitamins and probiotics is one therapeutic approach. To evaluate the effect of probiotics on vitamin absorption, we chose Lactobacillus rhamnosus CBT-LR5 treatment, which has resistance to vitamin C-inducible toxicity, with vitamins in high-fat diet (HFD)-induced obesity models. CBT-LR5 affected the absorption of micronutrients, such as ionic minerals and water-soluble vitamins. An increase in vitamin C absorption by CBT-LR5 enhanced the antioxidant response in HFD-induced obesity models. Increased vitamin B absorption by CBT-LR5 regulated lipid metabolism in HFD-induced obesity models. These favorable effects of CBT-LR5 on the absorption of vitamins should be investigated as candidate therapeutic target treatments for metabolic diseases.

Electrochemical properties of poly(4,4′-diaminodiphenyl sulfone) as a cathode material of lithium secondary batteries

Yun, Su-Ryeon,Kim, Kwang Man,Ko, Jang Myoun,Kang, Yongku,Ryu, Kwang Sun Springer-Verlag 2013 Polymer bulletin Vol.70 No.11

Synthesis and Control of the Shell Thickness of Polyaniline and Polypyrrole Half Hollow Spheres Using the Polystyrene Cores

Yun, Su-Ryeon,Kim, Gyeong-Ok,Lee, Chan Woo,Jo, Nam-Ju,Kang, Yongku,Ryu, Kwang-Sun Hindawi Limited 2012 Journal of nanomaterials Vol.2012 No.-

<P>Polyaniline (Pani) and polypyrrole (Ppy) half hollow spheres with different shell thicknesses were successfully synthesized by three steps process using polystyrene (PS) as the core. The PS core was synthesized by emulsion polymerization. Aniline and pyrrole monomers were polymerized on the surface of the PS core. The shells of Pani and Ppy were fabricated by adding different amounts of aniline and pyrrole monomers. PS cores were dissolved and removed from the core shell structure by solvent extraction. The thicknesses of the Pani and Ppy half hollow spheres were observed by FE-SEM and FE-TEM. The chemical structures of the Pani and Ppy half hollow spheres were characterized by FT-IR spectroscopy and UV-Vis spectroscopy. The shell thicknesses of the Pani half hollow spheres were 30.2, 38.0, 42.2, 48.2, and 52.4 nm, while the shell thicknesses of the Ppy half hollow spheres were 16.0, 22.0, 27.0, and 34.0 nm. The shell thicknesses of Pani and Ppy half hollow spheres linearly increased as the amount of the monomer increased. Therefore, the shell thickness of the Pani and Ppy half hollow spheres can be controlled in these ranges.</P>

Two-Dimensional Phosphorene-Derived Protective Layers on a Lithium Metal Anode for Lithium-Oxygen Batteries

Kim, Youngjin,Koo, Dongho,Ha, Seongmin,Jung, Sung Chul,Yim, Taeeun,Kim, Hanseul,Oh, Seung Kyo,Kim, Dong-Min,Choi, Aram,Kang, Yongku,Ryu, Kyoung Han,Jang, Minchul,Han, Young-Kyu,Oh, Seung M.,Lee, Kyu T American Chemical Society 2018 ACS NANO Vol.12 No.5

<P>Lithium-oxygen (Li-O<SUB>2</SUB>) batteries are desirable for electric vehicles because of their high energy density. Li dendrite growth and severe electrolyte decomposition on Li metal are, however, challenging issues for the practical application of these batteries. In this connection, an electrochemically active two-dimensional phosphorene-derived lithium phosphide is introduced as a Li metal protective layer, where the nanosized protective layer on Li metal suppresses electrolyte decomposition and Li dendrite growth. This suppression is attributed to thermodynamic properties of the electrochemically active lithium phosphide protective layer. The electrolyte decomposition is suppressed on the protective layer because the redox potential of lithium phosphide layer is higher than that of electrolyte decomposition. Li plating is thermodynamically unfavorable on lithium phosphide layers, which hinders Li dendrite growth during cycling. As a result, the nanosized lithium phosphide protective layer improves the cycle performance of Li symmetric cells and Li-O<SUB>2</SUB> batteries with various electrolytes including lithium bis(trifluoromethanesulfonyl)imide in <I>N,N</I>-dimethylacetamide. A variety of <I>ex situ</I> analyses and theoretical calculations support these behaviors of the phosphorene-derived lithium phosphide protective layer.</P> [FIG OMISSION]</BR>

유산균을 이용한 여주 열매 발효체의 항비만 및 항당뇨 증진 효과

곽상희(Sang Hee Kwak),안병철(Byung Chull An),안준영(Jun Young Ahn),이주성(Jusung Lee),정명준(Myung Jun Chung),류용구(Yongku Ryu) 한국미생물학회 2024 미생물학회지 Vol.60 No.1

본 연구에서는 다양한 생리적, 약리적 효능을 가지고 있는 것으로 알려진 여주의 효능을 더욱 증가시켜 고부가가치를 지닌 기능성 원료로써 활용하고자, 자사 특허 유산균 Lactobacillus plantarum CBT-LP3를 이용하여 발효시킨 후 그 추출물의 항당뇨 활성 및 항비만 활성을 평가하였다. 항당뇨 활성에 있어 여주 유산균 발효체는 3T3-L1 지방 세포의 2-deoxyglucose 흡수를 1 μM의 인슐린 보다 1.8 배 높게 증가시켰다. 반면 동일 농도의 비발효체 여주침지물은 대조군과의 유의적인 차이가 없었다. 뿐만 아니라 여주 유산균 발효체는 고농도 당독성에 대한 INS-1 췌장베타세포의 인슐린 분비 기능 부전효과를 완화시켰으며, 산화적 스트레스로부터 췌장베타세포의 생존율을 효과적으로 보호하는 것을 확인할 수 있었다. 항비만 측면에 있어 여주 유산균 발효체는 3T3-L1세포의 지방구 크기 감소와 갈색지방으로의 유전자 변화를 통해 항비만 효과를 나타내는 것으로 확인되며, 이는 본 논문에서 처음 밝혀졌다. 항당뇨, 항산화 활성물질로 알려진 γ-Aminobutyric acid(GABA)의 발효 전/후 함량을 분석한 결과, 유산균 발효를 통해 약 15%의 함량 증가가 확인되었다. 결론적으로 여주 열매는 CBT-LP3 유산균 발효에 의해 GABA의 함량과 항당뇨 및 항비만 효과가 증가되었으며, 이를 활용한 기능성 식품 또는 천연물 등으로 활용 가치가 있다 판단된다. As the prevalence of diabetes in South Korea continues to rise, there is a rapid increase in the prescription rate of oral hypoglycemic agents. Given that these medications need to be taken for a lifetime, there is a necessity to develop treatments that ensure long term safety and devoid of any side effects. Although much research has been conducted on the development of alternative drugs using plant-derived extracts, the active ingredients obtained through general extraction methods have limited effectiveness. Therefore, it is necessary to develop materials that maximize efficacy. Traditionally, Momordicae charantiae (MC) has been known for its effective anti-diabetic properties, so we utilized our own lactic acid bacteria, L. plantarum CBT-LP3, to enhance the anti-diabetic efficacy of MC. In the present study, we assessed the anti-diabetic and anti-obesity activities of bio-fermented M. charantiae fructus (BMC) using in vitro exams. The results demonstrated that the BMC increased glucose uptake through Glut4 induction in 3T3-L1 adipocytes. The BMC also effectively decreased lipid accumulation compared to untreated adipocytes. Furthermore, we found that the BMC substantially induced fat browning compared to untreated adipocytes. BMC, as another property, significantly increased reviving and recovering partially destroyed β cells by streptozotocin (STZ) treatment. This result suggests that BMC can protect and help to recover β cells against damage. In addition, BMC improved β cell dysfunction in high glucose conditions and protected against oxidative stress-induced diabetes. In conclusion, this study demonstrated that the fermentation of M. charantiae fructus using CBT-LP3 enhanced its anti-diabetic and anti-obesity effects.