<i>Haloactinopolyspora alkaliphila</i> sp. nov., and emended description of the genus <i>Haloactinopolyspora</i>

Zhang, Yong-Guang,Liu, Qing,Wang, Hong-Fei,Zhang, Dao-Feng,Zhang, Yuan-Ming,Park, Dong-Jin,Kim, Chang-Jin,Li, Wen-Jun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.6

<P>A facultatively alkaliphilic actinomycete strain, designated EGI 80088<SUP>T</SUP>, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088<SUP>T</SUP> formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained <SMALL>ll</SMALL>-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, summed feature 4 (iso-C<SUB>17 : 1</SUB>I/anteiso-C<SUB>17 : 1</SUB>B), iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The predominant menaquinone was MK-9(H<SUB>4</SUB>). The G+C content of the genomic DNA of strain EGI 80088<SUP>T</SUP> was 70.6 mol%. EGI 80088<SUP>T</SUP> showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour <I>Haloactinopolyspora alba</I> YIM 93246<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088<SUP>T</SUP> and <I>H. alba</I> YIM 93246<SUP>T</SUP> was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088<SUP>T</SUP> represents a novel species of the genus <I>Haloactinopolyspora</I>, for which the name <I>Haloactinopolyspora alkaliphila</I> sp. nov. (type strain EGI 80088<SUP>T</SUP> = BCRC 16946<SUP>T</SUP> = JCM 19128<SUP>T</SUP>) is proposed. The description of the genus <I>Haloactinopolyspora</I> has also been emended.</P>

Intravenous Tenecteplase for Acute Ischemic Stroke Within 4.5–24 Hours of Onset (ROSE-TNK): A Phase 2, Randomized, Multicenter Study

Wang Lu,Dai Ying-Jie,Cui Yu,Zhang Hong,Jiang Chang-Hao,Duan Ying-Jie,Zhao Yong,Feng Ye-Fang,Geng Shi-Mei,Zhang Zai-Hui,Lu Jiang,Zhang Ping,Zhao Li-Wei,Zhao Hang,Ma Yu-Tong,Song Cheng-Guang,Zhang Yi,Ch 대한뇌졸중학회 2023 Journal of stroke Vol.25 No.3

Background and Purpose Intravenous tenecteplase (TNK) efficacy has not been well demonstrated in acute ischemic stroke (AIS) beyond 4.5 hours after onset. This study aimed to determine the effect of intravenous TNK for AIS within 4.5 to 24 hours of onset. Methods In this pilot trial, eligible AIS patients with diffusion-weighted imaging (DWI)-fluid attenuated inversion recovery (FLAIR) mismatch were randomly allocated to intravenous TNK (0.25 mg/kg) or standard care within 4.5–24 hours of onset. The primary endpoint was excellent functional outcome at 90 days (modified Rankin Scale [mRS] score of 0–1). The primary safety endpoint was symptomatic intracranial hemorrhage (sICH). Results Of the randomly assigned 80 patients, the primary endpoint occurred in 52.5% (21/40) of TNK group and 50.0% (20/40) of control group, with no significant difference (unadjusted odds ratio, 1.11; 95% confidence interval 0.46–2.66; <i>P</i>=0.82). More early neurological improvement occurred in TNK group than in control group (11 vs. 3, <i>P</i>=0.03), but no significant differences were found in other secondary endpoints, such as mRS 0–2 at 90 days, shift analysis of mRS at 90 days, and change in National Institutes of Health Stroke Scale score at 24 hours and 7 days. There were no cases of sICH in this trial; however, asymptomatic intracranial hemorrhage occurred in 3 of the 40 patients (7.5%) in the TNK group. Conclusion This phase 2, randomized, multicenter study suggests that intravenous TNK within 4.5–24 hours of onset may be safe and feasible in AIS patients with a DWI-FLAIR mismatch.

rs10505474 and rs7837328 at 8q24 Cumulatively Confer Risk of Prostate Cancer in Northern Han Chinese

Zhang, Lin-Lin,Sun, Liang,Zhu, Xiao-Quan,Xu, Yong,Yang, Kuo,Yang, Fan,Yang, Yi-Ge,Chen, Guo-Qiang,Fu, Ji-Cheng,Zheng, Chen-Guang,Li, Ying,Mu, Xiao-Qiu,Shi, Xiao-Hong,Zhao, Fan,Wang, Fei,Yang, Ze,Wang, Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Aims: Genome-wide association studies (GWAS) have identified several risk variants for prostate cancer (pCa) mainly in Europeans, which need to be further verified in other racial groups. We selected six previously identified variants as candidates and to define the association with PCa in Northern Han Chinese. Methods: 749 subjects from Beijing and Tianjin in Northern China were included. Six variants (rs10505474, rs7837328, rs4242384, rs7813, rs486907 and rs1058205) were genotyped by high resolution melting (HRM) assays. The individual and cumulative contribution for of the risk of PCa and clinical covariates were analyzed. Results: Among the six candidate variants, onlyrs10505474, and rs7837328, both locating at 8q24 region, were associated with PCa in our population.rs10505474 (A) was associated with PCa ($OR_{recessive}=1.56$, p=0.006); and rs7837328 (A) was associated with PCa ($OR_{dominant}=1.38$, p=0.042/$OR_{recessive}=1.99$, p=0.003). Moreover, we observed a cumulative effects between them ($p_{trend}=2.58{\times}10^{-5}$). The joint population attributable risk showed the two variants might account for 71.85% of PCa risk. In addition, we found the homozygotes of rs10505474 (A) and rs7837328 (A) were associated with PCa clinical covariants (age at onset, tumor stage, respectively) ($p_{age}=0.046$, $P_{tumorstage}=0.048$). Conclusion: rs10505474 (A) and rs7387328 (A) at 8q24 are associated with PCa and cumulatively confer risk, suggesting the two variations could determine susceptibility to PCa in the Northern Chinese Han population.

Efficacy and Safety of OnabotulinumtoxinA in Patients With Neurogenic Detrusor Overactivity Caused by Spinal Cord Injury: A Systematic Review and Meta-Analysis

Guang-Ping Li,Xiao-Yan Wang,Yong Zhang 대한배뇨장애요실금학회 2018 International Neurourology Journal Vol.22 No.4

Purpose: OnabotulinumtoxinA (BoNT-A) is a promising therapy for treating neurogenic detrusor overactivity (NDO) in individuals with spinal cord injury (SCI). This systematic review and meta-analysis aimed to carry out an in-depth review and to make an objective estimation of the efficacy and safety of BoNT-A on NDO after SCI. Methods: The PubMed, Embase, and Cochrane databases were searched for all relevant articles published from 2001 to 2016 that referred to NDO, SCI, and BoNT-A or botulinum toxin A. All data were recorded in an Excel spreadsheet by 2 individual reviewers. Review Manager version 5.3 was used to carry out the meta-analysis. Results: This analysis included 17 studies involving 1,455 patients. Compared with placebo and baseline, BoNT-A was effective in increasing maximum cystometric capacity, volume at first involuntary detrusor contraction, cystometric bladder capacity (all P<0.00001), compliance (P=0.001), and the number of patients with complete dryness (P=0.0003), and decreasing detrusor pressure, the number of patients with no involuntary detrusor contractions, the maximum flow rate, the incidence of detrusor overactivity (all P<0.00001), and the number of urinary incontinence episodes (P=0.001). There were no statistically significant differences between doses of 200 U and 300 U or between injections into the detrusor and submucosa. There were no life-threatening adverse events. Conclusions: BoNT-A is effective and safe in treating NDO after SCI. There were no statistically significant differences between doses of 200 U and 300 U or between injecting into the detrusor and submucosa. However, more high-quality randomized controlled trials are still needed.

Evaluation of Carbonation Depth of High Volume Fly Ash Blended Concrete

Guang-Zhu Zhang(장광수),Xiao-Yong Wang(왕소용) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1

Kidney bean defense response induced by western flower thrips, Frankliniella occidentalis feeding and the attenuated effect of calcium ion chelator

Zhang Tao,Zhi Junrui,Liu Yong,Yue Wenbo,Zeng Guang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Calcium ion (Ca 2+ ) plays an important role in plant–insect interactions. Ethylene glycol-bis(2-aminoethylether)-N, N, N ′ , N ′ -tetraacetic acid (EGTA), a Ca 2+ chelator, can abolish Ca 2+ in plant cells following the disruption of plant physiological processes and the plant’s defense responses against biotic stress. To clarify whether EGTA pretreatment plants have an inhibitory effect on insect feeding induction, we determined variations in kidney bean (Phaseolus vulgaris (L., Fabales: Fabaceae)) plant defense responses induced by western flower thrips (WFT) (Frankliniella occidentalis (Pergande)) feeding and the reversal effects of EGTA. The results showed that WFT feeding can significantly activate the expression level of the jasmonic acid (JA) pathway marker genes allene oxide synthase (AOS) and lipoxygenase (LOX) and salicylic acid (SA) pathway marker genes phenylalanine ammonia lyase (PAL) and β-1,3 glucanase (PR-2), and induce the biosynthesis of JA and SA. However, pre treatment with EGTA showed varying degrees of reversal effects on JA and SA content and related gene expression levels with WFT feeding. These results indicate that WFT feeding can induce the biosynthesis of JA and SA and the expression of related genes to enhance the defense responses in kidney beans plants, while pretreatment with EGTA can attenuate the WFT-induced defense responses.

Design and testing of CFRP sleeve for a high-speed permanent magnet synchronous motor with surface-mounted rotor

Yong Zhou,Lei Tian,Sheng-Hua Gao,Jing-Wei Zhang,Lin-Ke Yang,Rui-Guang Xie 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.1

A high-speed (HS) permanent magnet (PM) synchronous motor (HSPMSM) with a carbon fiber-reinforced plastic (CFRP) protective sleeve in the surface-mounted rotor was explored in this study. In view of retaining the PMs at HS operations, the high-strength CFRP sleeve was designed on the basis of a process that could be summarized as follows. First, a multi-physics analysis of the rotor was conducted. The requirements to the CFRP sleeve were obtained from electromagnetic analysis, loss analysis, heat flow coupling computational fluid dynamics analysis, structural analysis, and rotor dynamics analysis. Second, the CFRP sleeve was designed by theoretical analysis. From the results, the thickness values of the carbon fibers in the circumferential and helical directions were chosen to be 4 and 1 mm, respectively, while the helical angle of carbon fibers was chosen to be 70°. Then, a plateshaped CFRP product and a cylindrical CFRP product were fabricated to verify the strength and stiffness of the CFRP sleeve. The performance test results show that the hoop strength and elastic modulus and the axial strength and elastic modulus of the plate-shaped CFRP products at 20 °C are 1963 MPa @ 156 GPa and 550 MPa @ 36 GPa, respectively. The hoop strain and axial strain of the cylindrical CFRP product under 35 MPa are about 3000 and 1900 με, which meet the design requirements of the HSPMSM.

Association of Six Susceptibility Loci with Prostate Cancer in Northern Chinese Men

Zhang, Yu-Rong,Xu, Yong,Yang, Kuo,Liu, Ming,Wei, Dong,Zhang, Yao-Guang,Shi, Xiao-Hong,Wang, Jian-Ye,Yang, Fan,Wang, Xin,Liang, Si-Ying,Zhao, Cheng-Xiao,Wang, Fei,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Zh Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background/Aim: Six prostate cancer (PCa) susceptibility loci were identified in a genome-wide association study (GWAS) in populations of European decent. However, the associations of these 6 single-nucleotide polymorphisms (SNPs) with PCa has remained tobe clarified in men in Northern China. This study aimed to explore the loci associated with PCa risk in a Northern Chinese population. Methods: Blood samples and clinical information of 289 PCa patients and 288 controls from Beijing and Tianjin were collected. All risk SNPs were genotyped using polymerase chain reaction (PCR)-high resolution melting curve technology and gene sequencing. Associations between PCa and clinical covariates (age at diagnosis, prostate-specific antigen [PSA], Gleason score, tumor stage, and level of aggressiveness) and frequencies of alleles and genotypes of these SNPs were analyzed using genetic statistics. Results: Among the candidate SNPs, 11p15 (rs7127900, A) was associated with PCa risk (P = 0.02, odds ratio [OR] = 1.64, 95% confidence interval [CI] = 1.09-2.46). Genotypes showed differences between cases and controls on 11p15 (rs7127900, A), 11q13 (rs7931342, T), and HNF1B (rs4430796, A) (P = 0.03, P = 0.01, and P = 0.04, respectively). The genotype TG on 11q13 (rs7931342, T) was positively associated with an increased Gleason score (P = 0.04, OR = 2.15, 95% CI = 1.02-4.55). Patients carrying TG on 17q24 (rs1859962, G) were negatively associated with an increased body mass index (BMI) (P = 0.03, OR = 0.44, 95% CI = 0.21-0.92) while those with AG on HNF1B (rs4430796, A) were more likely to have PSA increase (P = 0.002). Conclusion: Our study suggests that 11p15 (rs7127900, A) could be a susceptibility locus associated with PCa in Northern Chinese. Genotype TG on 11q13 (rs7931342, T) could be related to an increased Gleason score, AG on HNF1B (rs4430796, A) could be associated with PSA increase, and TG on 17q24 (rs1859962, G) could be negatively associated with an increased BMI in Chinese men with PCa.

Limited Unilateral Decompression and Pedicle Screw Fixation with Fusion for Lumbar Spinal Stenosis with Unilateral Radiculopathy : A Retrospective Analysis of 25 Cases

Zhang, Li,Miao, Hai-xiong,Wang, Yong,Chen, An-fu,Zhang, Tao,Liu, Xiao-guang The Korean Neurosurgical Society 2015 Journal of Korean neurosurgical society Vol.46 No.4

Objective : Lumbar spinal stenosis is conventionally treated with surgical decompression. However, bilateral decompression and laminectomy is more invasive and may not be necessary for lumbar stenosis patients with unilateral radiculopathy. We aimed to report the outcomes of unilateral laminectomy and bilateral pedicle screw fixation with fusion for patients with lumbar spinal stenosis and unilateral radiculopathy. Methods : Patients with lumbar spinal stenosis with unilateral lower extremity radiculopathy who received limited unilateral decompression and bilateral pedicle screw fixation were included and evaluated using visual analog scale (VAS) pain and the Oswestry Disability Index (ODI) scores preoperatively and at follow-up visits. Ligamentum flavum thickness of the involved segments was measured on axial magnetic resonance images. Results : Twenty-five patients were included. The mean preoperative VAS score was $6.6{\pm}1.6$ and $4.6{\pm}3.1$ for leg and back pain, respectively. Ligamentum flavum thickness was comparable between the symptomatic and asymptomatic side (p=0.554). The mean follow-up duration was 29.2 months. The pain in the symptomatic side lower extremity (VAS score, $1.32{\pm}1.2$) and the back (VAS score, $1.75{\pm}1.73$) significantly improved (p=0.000 vs. baseline for both). The ODI improved significantly postoperatively ($6.60{\pm}6.5$; p=0.000 vs. baseline). Significant improvement in VAS pain and ODI scores were observed in patients receiving single or multi-segment decompression fusion with fixation (p<0.01). Conclusion : Limited laminectomy and unilateral spinal decompression followed by bilateral pedicle screw fixation with fusion achieves satisfactory outcomes in patients with spinal stenosis and unilateral radiculopathy. This procedure is less damaging to structures that are important for maintaining posterior stability of the spine.

Effect of the opening of a butterfly valve on the dynamic evolution of cavitation

Guang Zhang,Ze Yong Wu,Ke Xin Wu,Yu Qiong Ou,Heuy Dong Kim,Zhe Lin 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

As the key control equipment for the transmission of fluid medium, processing valves are widely used in the transmission systems of fluid medium in energy, chemical industry, metallurgy and other fields, which play important roles in the stability and reliability of the system operation. When the flow cross-section is operated in a sudden change, the pressure decreases rapidly at the downstream, which leads to the cavitation in the processing valves. Cavitation makes serious erosion and damage on the valve core and pipeline surface, which makes the leakage and noise problems in processing valves. This seriously affects the regulation performance and lifetime of processing valves. In this article, numerical simulations were carried out to investigate the transient cavitation in a model butterfly valve. By considering effects of local pressure on formation of cavitation, a modified model for calculating the diameter of cavitation bubbles was derived. Effects of valve opening degree were investigated on the dynamic evolution of cavitation by analyzing formation, development and collapse of cavitation. The generation, development and collapse of single cavitation bubble was obtained and discussed in details to state the interaction between vortices and cavitation. Attached and quasiperiodic cavitation were observed and analyzed at different valve opening degrees in detail as well.