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      • 마우스 및 鷄 胎齡에 따른 骨·軟骨의 二重染色法

        전용혁,박선화,서영석 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.2

        Owing mainly to differences in race and fetal age, the procedures for dual staining of cartilage and bone in rodents, which have been used, cause a little trouble in applying then to the developing skeleton, Here, we present a simple and rapid procedure, a modification of previous ones, for staining of fetal skeletal from its formation until birth in mice and chick that are frequently used in teratology research. The procedure and results are summarized as follows; 1. Complete skinning and evisceration contributed to the whole procedure which yields high quality results. 2. Skinned and eviscerated specimen was stained and fixed in a mixture of 0.15% alizarin red S and 0.14% Alcian blue in ethanol and glacial acetic acid for 10 to 12 hours for cartilaginous skeleton but 15 to 20 hours for ossified one in both animals. 3. In time for tissue maceration; the longer, the better but it might separate the skeleton from the joint. So, time for it was prolonged stepwise from one hour to 2, 3, 4, 6 and 8 hours in 2% KOH solution for the fetus at 14th, 15th, 16th, 17th, 18th and 19th day of gestation respectively in mice; and in chick, 30 minutes, 1, 2 and 3 hours in 1% KOH solution for the fetus aged 6, 7, 8 and 9 day and 2, 3, 4, 5, 6, 8 and 10 hours in 2% solution for 10, 11, 12, 13, 14, 15 and over 16 day respectively. 4. Subsequently, specimens in both animals were placed in 1 : 1 distilled water and glycerin for 12 to 14 hours then in 1 : 3 solution for 24 hours for clearing and hardening prior to final storage in pure glycerin. High quality fetal specimens can be prepared for examination by this procedure within 30 hours and preserved for a long time.

      • KCI등재

        Development of educational software for beam loading analysis using pen-based user interfaces

        Suh, Yong S. Society for Computational Design and Engineering 2014 Journal of computational design and engineering Vol.1 No.1

        Most engineering software tools use typical menu-based user interfaces, and they may not be suitable for learning tools because the solution processes are hidden and students can only see the results. An educational tool for simple beam analyses is developed using a pen-based user interface with a computer so students can write and sketch by hand. The geometry of beam sections is sketched, and a shape matching technique is used to recognize the sketch. Various beam loads are added by sketching gestures or writing singularity functions. Students sketch the distributions of the loadings by sketching the graphs, and they are automatically checked and the system provides aids in grading the graphs. Students receive interactive graphical feedback for better learning experiences while they are working on solving the problems.

      • HEAT TRANSFER CHARACTERISTICS OF THREE PHASE FLUIDIZED BEDS

        KANG, YONG,SUH, IL SOON,KIM, S. D. 충남대학교 공업교육연구소 1986 論文集 Vol.8 No.3

        Heat transfer characteristics of two(liquid-gas, liquid-solid)and three(liquid-gas-solid)phase fluidized beds have been studied in a 15.2cm-ID column fitted with an axially mounted cylindrical heater. Effects of gas velocity (0-12 cm/s), liquid velocity (0-16 cm/s), particle size (1.7-8.0 mm) and liquid viscosity (0.001-0.039 Pa·s)on heat transfer coefficient were determined. The heat transfer coefficient increased with fluid velocities and particle size and it decreased with liquid viscosity in two and three phase fluidized beds. The bed porosity at which the maximum heat transfer occurred decreased with particle size but increased with liquid viscosity. The coefficient were correlated in terms of experimental variables. Modified Nusselt number from the present and previous studies has been correlated with modified Prandtl and Reynolds numbers.

      • Impact of Interlayer Processing Conditions on the Performance of GaN Light-Emitting Diode with Specific NiO<sub><i>x</i></sub>/Graphene Electrode

        Chandramohan, S.,Kang, Ji Hye,Ryu, Beo Deul,Yang, Jong Han,Kim, Seongjun,Kim, Hynsoo,Park, Jong Bae,Kim, Taek Yong,Cho, Byung Jin,Suh, Eun-Kyung,Hong, Chang-Hee American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.3

        <P>This paper reports on the evaluation of the impact of introducing interlayers and postmetallization annealing on the graphene/p-GaN ohmic contact formation and performance of associated devices. Current–voltage characteristics of the graphene/p-GaN contacts with ultrathin Au, Ni, and NiO<SUB><I>x</I></SUB> interlayers were studied using transmission line model with circular contact geometry. Direct graphene/p-GaN interface was identified to be highly rectifying and postmetallization annealing improved the contact characteristics as a result of improved adhesion between the graphene and the p-GaN. Ohmic contact formation was realized when interlayer is introduced between the graphene and p-GaN followed by postmetallization annealing. Temperature-dependent <I>I</I>–<I>V</I> measurements revealed that the current transport was modified from thermionic field emission for the direct graphene/p-GaN contact to tunneling for the graphene/metal/p-GaN contacts. The tunneling mechanism results from the interfacial reactions that occur between the metal and p-GaN during the postmetallization annealing. InGaN/GaN light-emitting diodes with NiO<SUB><I>x</I></SUB>/graphene current spreading electrode offered a forward voltage of 3.16 V comparable to that of its Ni/Au counterpart, but ended up with relatively low light output power. X-ray photoelectron spectroscopy provided evidence for the occurrence of phase transformation in the graphene-encased NiO<SUB><I>x</I></SUB> during the postmetallization annealing. The observed low light output is therefore correlated to the phase change induced transmittance loss in the NiO<SUB><I>x</I></SUB>/graphene electrode. These findings provide new insights into the behavior of different interlayers under processing conditions that will be useful for the future development of opto-electronic devices with graphene-based electrodes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-3/am3026079/production/images/medium/am-2012-026079_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am3026079'>ACS Electronic Supporting Info</A></P>

      • KCI등재

        용융탄산염형 연료전지 스택의 균질 물성치 추정에 관한 연구

        이상욱(Lee S.W.),서용석(Suh, Yong-S.) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.7

        용융탄산염형 연료전지 시스템의 성능 향상을 위해 핵심 부분인 스택을 수치적으로 모사할 필요성이 대두되 고 있다. 스택은 다양한 재질, 즉 금속, 세라믹, 폴리머 등으로 이루어져 있고 또한 단위전지 수백 장이 적층되어 제 작되기 때문에 이를 전부 세세히 모델링하여 고려하는 것은 거의 불가능하다. 이에 따라 스택 전체를 하나의 균질한 물질로 대체할 수 있는 균질 물성치 도출이 요구되고 있다. 본 논문에서는 단위전지 압축률을 도입하고 모든 압축 변 형이 분리판과 매트릭스에서만 일어난다는 등의 몇 가지 기본 가정 하에서 스택의 두 가지 영역, 즉 활성 영역과 매 니폴드 영역에서 각각 균질 물성치를 추정하였다. 추정된 물성치를 실제 문제에 적용하여 성공적인 결과를 얻을 수 있었다. The performance and efficiency of a Molten Carbonate Fuel Cell system will improve with the aids of numerical simulations such as finite element analysis. For best simulation results, the virtual model must accurately reflect the actual model including the material properties. It is very difficult, however, to make a detailed numerical model of the stack that consists of hundreds of layers of unit cells composed of various materials like metal, ceramics, polymer, etc. Instead, a practical approach is to find a homogenized material property of the stack as a whole as an approximate replacement. In this paper, the compression ratio of a unit cell is introduced, and a new method is proposed to estimate the homogeneous material properties for both the active and the manifold regions of the stack under the assumption that the compressive deformation occurs only at the separators and matrices in the unit cells. The estimated properties are applied successfully to simulating an actual stack.

      • SCISCIESCOPUS

        Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.

        Lee, Hyun Yong,Abeyrathne, E D N S,Choi, Inwook,Suh, Joo Won,Ahn, Dong Uk Poultry Science Association, etc 2014 Poultry science Vol.93 No.10

        <P>A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was adjusted to 4.0 and the precipitate was removed by centrifugation. The supernatant was collected and then heat-treated at 70C for 30 min and centrifuged to remove impurities. The supernatant containing phosvitin was collected, had salts removed, and was concentrated and then freeze-dried. The supernatant from the centrifugation of diluted egg yolk was diluted again with 3 volumes of DW, and the precipitate was removed by centrifugation. The resulting supernatant was concentrated using ultrafiltration and then IgY was precipitated using 20% saturated (NH4)2SO4+ 15% NaCl (wt/vol). The precipitant was collected after centrifugation at 3,400 g for 30 min at 4C and dissolved with DW, had salts removed, and then was freeze-dried. The purity of separated phosvitin and IgY was checked using SDS-PAGE and the proteins were verified using Western blotting. The purity of phosvitin and IgY was 97.2 and 98.7%, and the yield was 98.7 and 80.9%, respectively. The ELISA results indicated that the activities of separated IgY and phosvitin were 96.3 and 98.3%, respectively. This study proved that both phosvitin and IgY can be separated in sequence from egg yolk without using an organic solvent. Also, the method is very simple and has a high potential for scale-up processing.</P>

      • SCISCIESCOPUS

        Reperfusion Injury on Magnetic Resonance Imaging After Carotid Revascularization

        Cho, A-Hyun,Cho, Yong-Pil,Lee, Deok Hee,Kwon, Tae-Won,Kwon, Sun U.,Suh, Dae-Chul,Kim, Jong S.,Kang, Dong-Wha American Heart Association, Inc. 2014 Stroke Vol.45 No.2

        <P><B>Background and Purpose—</B></P><P>Reperfusion injury can be revealed as delayed gadolinium enhancement in the subarachnoid space on a fluid-attenuated inversion recovery image, which is designated as a hyperintense acute reperfusion marker (HARM). We sought to investigate the occurrence and predictors of HARM and its association with new ischemic infarcts after carotid revascularization.</P><P><B>Methods—</B></P><P>Forty-five patients who underwent carotid endarterectomy or stenting were prospectively enrolled. Diffusion-weighted imaging and fluid-attenuated inversion recovery were performed before and 24 hours after the procedures. Postprocedural fluid-attenuated inversion recovery was done after intravenous gadolinium injection.</P><P><B>Results—</B></P><P>HARM was observed in 8 (17.8%) patients. Of these, 4 patients showed neurological deterioration associated with HARM. These symptomatic HARMs disappeared on follow-up magnetic resonance imaging. Patients with symptomatic HARM were older (<I>P</I>=0.010) and had more frequent leukoaraiosis (<I>P</I>=0.012) and higher postprocedural systolic blood pressure (<I>P</I>=0.025) than those without. New brain infarcts on postprocedural diffusion-weighted imaging were identified in 13 (28.9%) patients. HARM was not associated with new infarcts.</P><P><B>Conclusions—</B></P><P>HARM after carotid revascularization is not uncommon. Symptomatic HARM was associated with old age, underlying leukoaraiosis, and postprocedural high blood pressure.</P>

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