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식용 해조류 톳(Hizikia fusiformis) 유래 항산화 활성분획물의 산화적 손상이 유도된 인간피부섬유아세포 보호 효과
Yong Ri Cui,김현수,제준건,Lei Wang,오재영,Liu Jia,전유진 한국수산과학회 2019 한국수산과학회지 Vol.52 No.1
We investigated the protective effects of antioxidant fractions from a 70% ethanolic extract of Hizikia fusiformis in human dermal fibroblasts (HDFs). Powdered H. fusiformis was extracted with 70% ethanol and then partitioned into three fractions according to polarity using n-hexane (HFH), chloroform (HFC), and ethyl acetate (HFEA). Antioxidant activity was observed in HFEA at 0.66 mg/mL based on the half maximal inhibitory concentration (IC50) of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and at 0.24 mg/mL based on alkyl radical scavenging. The protective effects of the HFEA antioxidant fraction against 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH)-damaged HDFs and the expression of Type I procollagen in HDFs were examined. HFEA caused the proliferation of HDFs with and without AAPH treatment and protected against AAPH damage to HDFs in a dose-dependent manner (50-200 μg/mL). This implies that the antioxidant properties of the fractions depended on their proliferative and protective effects. The HFEA antioxidant fraction had significant effects and caused the dose-dependent expression of Type I procollagen, an important anti-wrinkle protein, in HDFs. In conclusion, antioxidant substances in H. fusiformis were found in the ethyl acetate fraction, and the resulting HFEA may have cosmetic applications.
Wang, Lei,Cui, Yong Ri,Yang, Hye-Won,Lee, Hyo Geun,Ko, Ju-Young,Jeon, You-Jin The Korean Society of Fisheries and Aquatic Scienc 2019 Fisheries and Aquatic Sciences Vol.22 No.5
Background: In the present study, the skin-whitening effects of a marine-sourced mixture that includes a fucoidanrich extract of Undaria pinnatifida (UPEF), a phlorotannin-rich extract of Ecklonia cava (ECE), and glycosaminoglycans (GAGs) from sea squirt skin were investigated. Methods: The whitening effects of the mixture and its components were evaluated by measuring the inhibition of mushroom tyrosinase and melanin synthesis in alpha-melanocyte-stimulating hormone (${\alpha}$-MSH)-stimulated B16F10 melanoma cells. Results: Each component alone markedly inhibited mushroom tyrosinase in a dose-dependent manner, and in ${\alpha}$-MSH-stimulated B16F10 cells, they inhibited melanin synthesis and were cytotoxic. However, the whitening effects of UPEF, ECE, and GAGs in combination were greater than those of each component alone. A mixture in the ratio of 4:5:1 (UEG-451) showed the strongest activity without cytotoxicity. Further study suggested that UEG-451 inhibits ${\alpha}$-MSH-stimulated melanogenesis in B16F10 cells by downregulating tyrosinase and tyrosinase-related proteins, such as TRP-1 and TRP-2, via the inhibition of MITF expression. Conclusions: These results suggest that mixing the different components at optimum ratios might be an effective way to improve their bioactivities and reduce toxicity and that UEG-451 possesses strong whitening effects that could be used in the cosmetic industry.
Lei Wang,Yong Ri Cui,양혜원,이효근,Ju-Young Ko,전유진 한국수산과학회 2019 Fisheries and Aquatic Sciences Vol.22 No.2
Background: In the present study, the skin-whitening effects of a marine-sourced mixture that includes a fucoidanrich extract of Undaria pinnatifida (UPEF), a phlorotannin-rich extract of Ecklonia cava (ECE), and glycosaminoglycans (GAGs) from sea squirt skin were investigated. Methods: The whitening effects of the mixture and its components were evaluated by measuring the inhibition of mushroom tyrosinase and melanin synthesis in alpha-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Results: Each component alone markedly inhibited mushroom tyrosinase in a dose-dependent manner, and in α- MSH-stimulated B16F10 cells, they inhibited melanin synthesis and were cytotoxic. However, the whitening effects of UPEF, ECE, and GAGs in combination were greater than those of each component alone. A mixture in the ratio of 4:5:1 (UEG-451) showed the strongest activity without cytotoxicity. Further study suggested that UEG-451 inhibits α-MSH-stimulated melanogenesis in B16F10 cells by downregulating tyrosinase and tyrosinase-related proteins, such as TRP-1 and TRP-2, via the inhibition of MITF expression. Conclusions: These results suggest that mixing the different components at optimum ratios might be an effective way to improve their bioactivities and reduce toxicity and that UEG-451 possesses strong whitening effects that could be used in the cosmetic industry.
Lei Wang,Won Woo Lee,Hye-Won Yang,Bo Mi Ryu,Yong Ri Cui,Seung-Cheol Lee,Tae-Gee Lee,You-Jin Jeon 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.4
Citrus pomace (CP) is a by-product occurred during juice or other products processing. The enormous amount of CP caused serious environmental issues. However, CP is rich in a variety of bioactive compounds. In the present study, a water extract of CP (CPW) was prepared from the by-product and the in vitro and in vivo antioxidant activities of CPW were investigated. The in vitro antioxidant activities of CPW were evaluated by measuring the free radical scavenging activity and protective effects against 2,2-azobis(2-amidinopropane) hydrochloride (AAPH)-induced oxidative stress in Vero cells. CPW scavenges 1,1-diphenyl-2-picrylhydrazyl, alkyl, and hydroxyl radicals at IC50 of 0.16±0.00, 0.31±0.01, and 0.86 ±0.02 mg/mL, respectively. In addition, CPW improved cell viability and scavenged intracellular reactive oxygen species (ROS) in AAPH-stimulated Vero cells in a dose-dependent manner. The in vivo antioxidant activities of CPW were investigated in a model of AAPH-induced zebrafish embryos. CPW significantly improved the survival rates and reduced heartbeat rates in AAPH-stimulated zebrafish. Furthermore, the intracellular ROS and cell death levels were remarkably decreased in CPW-treated zebrafish. Therefore, the present results indicated that CPW possesses potent in vitro and in vivo antioxidant properties and could be a potential ingredient used in food, pharmaceutical, and cosmetic industries.
전유진,박영진,오재영,I.P. Shanura Fernando,K. K. Asanka Sanjeewa,강민철,Yong Ri Cui,이효근,고재영,이원우,WANGLEI 한국키틴키토산학회 2018 한국키틴키토산학회지 Vol.23 No.2
In the present study, Sargassum fulvellum was extracted by enzyme-assistant extraction and the proximate compositions of these extracts were determined. The extraction yields of enzyme-assistant extraction were improved 4.67 to 9.67% compared to water extraction. The free radical scavenging activity of these extracts was evaluated using Electron Spin Resonance (ESR) spectroscopy and the cytotoxicity of these extracts was evaluated by measuring the viability of Vero cells. The Alcalase extract (SFA-2), Celluclast extract (SFC), Kojizyme extract (SFK), and Neutrase extract (SFN) were shown stronger free radical scavenging activity and non-toxicity in Vero cells compared with other extracts. The protective effects of SFA-2, SFC, SFK, and SFN against AAPH-induced oxidative stress in Vero cells were evaluated. SFC showed the strongest protective effect that indicated in scavenging 35.33, 38.75, and 47.69% intracellular reactive oxygen species (ROS) and improving 5.89, 8.36, and 12.13% viability at the concentration of 25, 50, and 100 μg/mL in AAPH-induced Vero cells. Therefore, this study demonstrated that enzyme-assistant extraction is an effective extraction method to extract antioxidant from algae, and SFC possesses strong antioxidant activity may be used as an ideal ingredient in pharmaceutical and cosmeceutical industries.