MiRNA Synergistic Network Construction and Enrichment Analysis for Common Target Genes in Small-cell Lung Cancer

Zhang, Tie-Feng,Cheng, Ke-Wen,Shi, Wei-Yin,Zhang, Jin-Tao,Liu, Ke-Di,Xu, Shu-Guang,Chen, Ji-Quan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background: Small-cell lung cancer (also known as SCLC) is an aggressive form and untreated patients generally die within about 3 months. To obtain further insight into mechanism underlying malignancy with this cancer, an miRNA synergistic regulatory network was constructed and analyzed in the present study. Method: A miRNA microarray dataset was downloaded from the NCBI GEO database (GSE27435). A total of 546 miRNAs were identified to be expressed in SCLC cells. Then a miRNA synergistic network was constructed, and the included miRNAs mapped to the network. Topology analysis was also performed to analyze the properties of the synergistic network. Consequently, we could identified constitutive modules. Further, common target genes of each module were identified with CFinder. Finally, enrichment analysis was performed for target genes. Results: In this study, a miRNA synergistic network with 464 miRNAs and 2981 edges was constructed. According to the topology analysis, the topological properties between the networks constructed by LC related miRNAs and LC unrelated miRNAs were significantly different. Moreover, a module cilque0 could be identified in our network using CFinder. The module included three miRNAs (hsa-let-7c, hsa-let-7b and hsa-let-7d). In addition, several genes were found which were predicted to be common targets of cilque0. The enrichment analysis demonstrated that these target genes were enriched in MAPK signaling pathways. Conclusions: Although limitations exist in the current data, the results uncovered here are important for understanding the key roles of miRNAs in SCLC. However, further validation is required since our results were based on microarray data derived from a small sample size.

Clerodane furanoditerpenoids from the stems of Tinospora sinensis

Jun-Sheng Zhang,De-Feng Xu,Yin-Yin Wang,Ren-Fen Ma,Hua Zhang 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.5

One new clerodane-type furanoditerpenoidtinosinoid A ( 1 ) and nine new nor-clerodane analogs tinosinoids B–J ( 2 − 10 ) have been isolated from the stems ofTinospora sinensis . The structures of the new compoundswith absolute confi gurations have been elucidated by spectroscopicmeans, including MS, NMR and ECD techniques,as well as chemical correlation. Compound 1 is a rare sulfurcontainingclerodane diterpenoid incorporating a 2-mercaptoethanolunit via a thioether bond, while compounds 4 / 5and 9 represent two pairs of unusual equilibrium regioisomersthrough an interesting intramolecular transesterifi cation. Our bioassays established that 1 and 8 displayed moderateantiproliferative eff ects against two human tumor celllines, and 9 and 10 showed signifi cant α -glucosidase inhibitoryactivities. A kinetics study revealed that compound 10was a noncompetitive α -glucosidase inhibitor, and its possiblebinding mode to the enzyme was further probed bymolecular docking experiments.

Protective Effects of Gypenosides against Fatty Liver Disease Induced by High Fat and Cholesterol Diet and Alcohol in Rats

Renan Qin,Hong Nie,Jianyu Zhang,Chuyuan Li,Xiaoqi Zhang,Aihua Xiong,Feng Huang,Zhen Yin,Kongyan Li,Wenyu Qin,Mingzhen Chen,Shubing Zhang,Lingyi Liang,Huiye Zhang,Wencai Ye 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.7

In the present study, the protective effects of gypenosides from Gynostemma pentaphyllum on fatty liver disease (FLD) were examined in rats treated with high fat and cholesterol diet and alcohol. Male SD rats were divided into seven groups: control, model, lovastatin, silymarin, gypenosides high-, medium- and low-treatment groups. The latter 6 groups were fed high-fat and cholesterol diet and administered alcohol intragastricly once a day. Body weight was measured every week for 10 weeks, and the hepatic index was measured after 10 weeks. Compared with model group, levels of serum triglyceride (TG), total cholesterol (TC), free fatty acid (FFA), and low density lipoprotein cholesterol (LDL-C) level, malondialdehyde (MDA), serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, and hepatocyte apoptosis were significantly decreased in gypenosides groups; while serum high density lipoprotein cholesterol (HDL-C), superoxide dismutase (SOD) activity in both serum and hepatic tissue and mRNA and protein level of peroxisome proliferator-activated receptor α (PPAR-α) were significantly increased. Moreover, hepatic steatosis and mitochondrial damage were improved. These results suggested that gypenosides could prevent liver fatty degeneration in fatty liver disease through modulating lipid metabolism, ameliorating liver dysfunction and reducing oxidative stress.

TCA: A Trusted Collaborative Anonymity Construction Scheme for Location Privacy Protection in VANETs

Zhang, Wenbo,Chen, Lin,Su, Hengtao,Wang, Yin,Feng, Jingyu Korean Society for Internet Information 2022 KSII Transactions on Internet and Information Syst Vol.16 No.10

As location-based services (LBS) are widely used in vehicular ad-hoc networks (VANETs), location privacy has become an utmost concern. Spatial cloaking is a popular location privacy protection approach, which uses a cloaking area containing k-1 collaborative vehicles (CVs) to replace the real location of the requested vehicle (RV). However, all CVs are assumed as honest in k-anonymity, and thus giving opportunities for dishonest CVs to submit false location information during the cloaking area construction. Attackers could exploit dishonest CVs' false location information to speculate the real location of RV. To suppress this threat, an edge-assisted Trusted Collaborative Anonymity construction scheme called TCA is proposed with trust mechanism. From the design idea of trusted observations within variable radius r, the trust value is not only utilized to select honest CVs to construct a cloaking area by restricting r's search range but also used to verify false location information from dishonest CVs. In order to obtain the variable radius r of searching CVs, a multiple linear regression model is established based on the privacy level and service quality of RV. By using the above approaches, the trust relationship among vehicles can be predicted, and the most suitable CVs can be selected according to RV's preference, so as to construct the trusted cloaking area. Moreover, to deal with the massive trust value calculation brought by large quantities of LBS requests, edge computing is employed during the trust evaluation. The performance analysis indicates that the malicious response of TCA is only 22% of the collaborative anonymity construction scheme without trust mechanism, and the location privacy leakage is about 32% of the traditional Enhanced Location Privacy Preserving (ELPP) scheme.

The electrocatalysis of Mn-Co3O4/CeO2@C particles with different Ce content modified Ti/PbO2 anode and its application for copper electrodeposition

Yin Zihang,He Ruibo,Nie Fei,Wei Zhen,Jia Bo,Feng Qing,Fu Xiaolong,Zhang Wenyan 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.12

The oxygen evolution kinetics of industrial copper electrodeposition is slow, resulting in low electrocatalytic activity and high energy consumption. In this work, a quaternary composite of carbon coated active particles containing Mn, Co and Ce were prepared (Mn-Co3O4/CeO2@C), and Ti/Sb-SnO2/PbO2 electrode doped with these active particles was prepared by co-electrodeposition. The microstructure and chemical composition of the electrode was characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and X-ray diffractometry (XRD). Linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS) and Tafel polarization curve (Tafel) were used to study the electrochemical properties of anode materials. The results showed that the doping of Mn-Co3O4/CeO2@C active particles promoted the crystal transition of PbO2, decreased the average grain size, and the doping of Ce increases the average valence state of Co. The modified titanium electrode showed excellent catalytic activity of the oxygen evolution reaction (OER) characteristics. The overpotential of the doped Ti/Sb-SnO2/PbO2 anode was only 453 mV when the current density was 20 mA cm−2 in 0.5 M H2SO4 solution, which is 508 mV lower than that of the undoped Ti/Sb-SnO2/PbO2 anode. In simulated copper electro-deposition experiments, the cell voltage was reduced by about 400 mV, compared to the undoped Ti/Sb-SnO2/PbO2 electrode.

Concentration-Controlled and Phytic Acid-Assisted Synthesis of Self-Assembled LiFePO4 as Cathode Materials for Lithium-Ion Battery

Yin Li,Keyu Zhang,Zhengjie Chen,Yunke Wang,Li Wang,Feng Liang,Yaochun Yao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.02

The olivine LiFePO4 with various morphologies and different growth lattice planes was prepared by a controllable hydrothermal method with changing precursor concentration and using phytic acid as phosphorus source. The microstructure, crystal orientation and electrochemical performance of the prepared samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM) and charge–discharge tests. The results show that the morphologies of all samples change from spindle-like to hierarchical plate-like and then to long plate-like shape, and the main exposed facets transform from (100) to (001). This indicates that the precursor concentration and phytic acid play important roles in exposing facets and controlling the morphology of LiFePO4. In order to illustrate these phenomena, a reasonable assembly process is provided and the formation is explained. Li ion diffusion coefficient along [100] and [001] directions was calculated by using electrochemical impedance spectroscopy (EIS). The results show that the diffusion coefficient of (100) facet is higher than that of (001) facet, indicating a good electrochemical performance for (100) facet. In addition, the capacity test is carried out, which also confirms the above results. With the precursor concentration of 0.5 M, the obtained LiFePO4 with self-assembled hierarchical structure, smaller size and (100) facet shows the best electrochemical performance: 162.1 mAh/g at 0.1C and 112.4 mAh/g at 10 C. Using phytic acid as phosphorus source and controlling precursor concentration to prepare high performance LiFePO4 open up a new prospect for the production of cathode materials for lithium ion batteries.

Crude Dietary Polysaccharide Fraction Isolated from Jackfruit Enhances Immune System Activity in Mice

Yin-Feng Tan,Hai-Long Li,Wei-Yong Lai,Jun-Qing Zhang 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.7

Crude polysaccharides (PSs) were isolated from the fruit pulp of jackfruit, and their chemical composition determined and evaluated for an immune regulatory activity in mice. The PSs were isolated from water extracts of jackfruit pulp ( JFP) using the ethanol precipitation method. The resulting precipitates were further purified by dialysis and protein depletion by the Sevage method. The phenol–sulfuric method was used to determine the content of the PSs. The composition of PSs was determined by the Sephadex-G200 column chromatography and high-performance liquid chromatography methods. The thymus index and macrophage phagocytic function methods in mice were used to evaluate the immune regulatory activity of JFP-PSs. The JFP-PSs content in jackfruit was about 21% (w/w) and the yield of crude PSs was 3.91%. The single molecular mass weight PS was the main constituent of JFP-PSs. The major monosaccharide residues were rhamnose, glucose, galactose, and arabinose. The JFP-PSs enhanced the thymus weight index and the phagocytic rate after 30 days of subchronic p.o. administration to mice at 4.5 mg/kg. The JFP contains single molecular PS and JFP-PS has immunestimulating activities in mice. These data suggest that at least some of the traditional uses of JFP can be ascribed to its immunomodulatory effects.

An Enhanced Searching Electromagnetism-like Mechanism Algorithm for Global Optimization Problem

Zhang Ruiqi,Yin Feng 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.8

A global optimal algorithm derived from electromagnetism-like mechanism (EM), called as enhanced searching electromagnetism-like (ESEM) algorithm, was developed in this paper. The original EM is a meta-heuristic algorithm utilizing an attraction-repulsion mechanism (called as force F) to move sample points towards optimality in global optimization problems. Compared to the original algorithm, the best historical visited positions of each point were added into the search process in the improved algorithm. In ESEM, the search direction and step length of points were determined together by its best previously visited position, best point in current swarm and total force F. Preliminary experiments showed that additional best historical positions can help to improve the convergence property. More importantly, the improved searching mechanism can effectively solve the problem of stagnation of the original algorithm caused by too small values of force F.

Distinct effects of Al³⁺ doping on the structure and properties of hexagonal turbostratic birnessite: A comparison with Fe³⁺ doping

Yin, Hui,Kwon, Kideok D.,Lee, Jin-Yong,Shen, Yi,Zhao, Huaiyan,Wang, Xiaoming,Liu, Fan,Zhang, Jing,Feng, Xionghan Elsevier 2017 Geochimica et cosmochimica acta Vol.208 No.-

<P><B>Abstract</B></P> <P>Hexagonal turbostratic birnessite, one of the most reactive Mn oxide minerals, is ubiquitous throughout the ocean floor to the surface environment. During its crystallization, birnessite may coexist with Al<SUP>3+</SUP>, which is the third most abundant crustal element. However, interactions of Al<SUP>3+</SUP> with birnessite compared to the transition metal (TM) ions have rarely been explored thus far. This study examines the structure and properties of Al<SUP>3+</SUP>-doped hexagonal turbostratic birnessite to obtain insights into the interaction of metal cations with birnessite-like minerals in natural environments. For Al<SUP>3+</SUP>-incorporated birnessite, the crystal chemistry of Al<SUP>3+</SUP>, as well as alteration in the mineral structure, physicochemical properties, and reactivity toward the sorption of Pb<SUP>2+</SUP>/Zn<SUP>2+</SUP> is investigated by powder X-ray diffraction, chemical analysis, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray absorption spectroscopy. Electronic structure calculations based on density functional theory (DFT) are further combined to aid in the experimental interpretation of Al<SUP>3+</SUP> incorporation. As a comparative system, Fe<SUP>3+</SUP>-coprecipitated birnessite is also examined. Under the experimental conditions used, only a small amount of Al<SUP>3+</SUP> is incorporated into birnessite, with a final Al/(Al+Mn) molar ratio of ∼0.07, whereas Fe<SUP>3+</SUP> is incorporated into birnessite with a final Fe/(Fe+Mn) molar ratio of up to ∼0.21. Irrespective of metal type, the incorporation of a metal cation significantly alters the physicochemical properties of birnessite, such as decrease in the thickness of crystals along the <B>c</B> <SUP>∗</SUP> axis and coherent scattering domain sizes in the <B>a</B>–<B>b</B> plane and the Mn average oxidation state, increase in the specific surface area and the total amount of hydroxyl groups, in which the contents of hydroxyl groups around vacancies are decreased. The lattice parameters in the <B>a</B>–<B>b</B> plane tend to decrease in Al–incorporated birnessites but first significantly decrease and then increase in Fe-incorporated birnessites. In Fe-incorporated birnessites, ∼32–50% of the total Fe<SUP>3+</SUP> is located inside the Mn octahedral sheets (INC species). In Al–incorporated birnessites, the edge- and corner-sharing Mn–Mn distances gradually decrease. Density function theory (DFT) computation results support that the dominant species in Al–birnessite is a triple-corner-sharing complex on vacancies. The DFT geometry optimization further demonstrates that the in-plane cell size experimentally observed for these birnessites depends on not only the metal type but also its position in the mineral. The Al- or Fe-birnessites exhibit significantly increased adsorption capacities for Pb<SUP>2+</SUP> but reduced capacities for Zn<SUP>2+</SUP>. The metal incorporation effects on the chemical reactivity are discussed with the observed changes in the particle size and available vacancy sites.</P>

N6-Methyladenosine modification (m6 A) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats

Yin Ronghuan,Yin Ronglan,Bai Man,Fan Yixing,Wang Zeying,Zhu Yubo,Zhang Qi,Hui Taiyu,Shen Jincheng,Feng Siyu,Bai Wenlin 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4

Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6Adeficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHFstem cells. We further demonstrated that the internal m6A modification within circRNAZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNAZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis.