從影響層面着中國小說的歷史定位 : 以靑心才人 <<金雲翹傳>>爲例

陳益源 中國語文硏究會 1998 中國語文論叢 Vol.15 No.-

Liposome-mediated Induction of Apoptosis of Human Hepatoma Cells by C-Myc Antisense Phosphorothioate Oligodeoxynucleotide and 5-Fluorouracil

Yuan, Yuan,Cai, Hui,Yang, Xiao-Jun,Li, Wei,He, Jin,Guo, Tian-Kang,Chen, Yi-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: The aim of this study was to investigate the effect of a c-myc antisense oligodeoxynucleotide and 5-fluorouracil on the expression of c-myc, invasion and proliferation of HEPG-2 liver cancer cells. Materials and Methods: HEPG-2 cells were treated with lipiosome-mediated c-myc ADSON and 5-fluorouracil. The proliferation inhibition rate and invasion were measured by MTT and invasion assay, respectively. Cell apoptosis was detected by flow cytometry and expression of c-myc by RT-PCR and immunohistochemistry. Results: The proliferation inhibition rate was significantly higher in the antisense oligodeoxynucleotide added-5-fluorouracil group than single antisense oligodeoxynucleotide or 5-fluorouracil group (p<0.05). G0/G1 cells in the antisense oligodeoxynucleotide group and S cells in the 5-fluorouracil groups were significantly increased than that in the control group, respectively (P<0.01). The amplification strips of PCR products in 5-FU, ASODN and combination groups were significantly weaker than that in the control group (P<0.01). The percentage of c-myc-protein-positive cells were significantly lower in antisense oligodeoxynucleotide, 5-fluorouracil and combination groups than that in the control group (P<0.01). Conclusions: A liposome-mediated c-myc antisense oligodeoxynucleotide and 5-fluorouracil can inhibit the proliferation and invasion of liver cancer cells by reducing the expression of c-myc. A c-myc antisense oligodeoxynucleotide can increase the sensitivity of liver cancer cells to 5-fluorouracil and decrease the dosage of the agent necessary for efficacy, providing an experimental basis for the clinical therapy of liver cancer.

Efficient slope reliability analysis using a surrogate-assisted normal search particle swarm optimization algorithm

Yuan Yi-li,Hu Chang-ming,LI LIANG,Hou Xu-hui 한국CDE학회 2024 Journal of computational design and engineering Vol.11 No.1

Sampling of training data is the most important step in active learning slope reliability analysis, which controls the analysis accuracy. In this study, a novel surrogate-assisted normal search particle swarm optimization (SANSPSO) was proposed to enhance the accuracy and robustness of existing methodologies. In SANSPSO, the sampling process was considered a minimum problem with an objective function defined as the absolute value of the performance function. Initiated with a normal search paradigm and supplemented by three algorithm strategies, this approach seeks to preserve the continuity of the solution while refining the algorithm’s efficacy and efficiency. To reduce computation cost, surrogate-assistance was used, in which a surrogate model substitutes the objective function in most iterations. This surrogate model evolves during the iteration process and ultimately replaces the actual performance function within Monte Carlo simulation. Finally, this study presents a comparative study with five state-of-the-art methods across four explicit problems and three engineering cases, where test data suggest that the SANSPSO methodology yields a 20% improvement in accuracy and a 30% rise in stability under different dimensional problems relative to the most efficacious of the alternate methods assessed because of the improved and more consistent prediction of limit state function. These findings substantiate the validity and robustness of the SANSPSO approach.

Effect of Workpiece Atom Diffusion into CBN Tool on its Mechanical Properties in Cutting Ni–Fe–Cr Alloy Based on Molecular Dynamics Simulation

Yi Hang Fan,Wen Yuan Wang,Zhao Peng Hao,Hengyi Yuan 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.4

CBN (Cubic Boron Nitride) tool is suitable for precision machining of nickel-based alloy due to its high hardness and chemical inert. Nickel-based alloys are difficult-to-cut material. When high-speed cutting nickel-based alloys with CBN tool, the temperature at tool-workpiece interface is very high. The atoms in workpiece material diff use into the tool surface and change the performance of cutting tool, resulting in diffusion wear. Inconel 718, which is one kind of widely used nickel-based alloy, is mainly composed of nickel (Ni), iron (Fe) and chromium (Cr). In this paper, the cutting model which describes the single crystal CBN tool cutting Ni–Fe–Cr three-component alloy, and the compression and impact model of CBN tool crystal are established by using molecular dynamics (MD) method to study the diffusion of Ni, Fe and Cr atoms into CBN tool and the effects of diffusion on the properties of CBN crystal tool. The results show that the diffusion of Ni, Fe and Cr atoms into CBN tool decreases the compressive strength and impact toughness of CBN crystals and the effects degree of atom diffusion on the properties of CBN crystals are different.

Complementary transcriptomic and proteomic analysis of Bombyx mori middle silk glands reveals a predominant ribosome-biogenesis regulating network during silkworm yellow-cocoon color formation

Yuan Yi,Xiao Rui,Ge Qi,Taha Rehab Hosny,Chen Keping 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The human macular carotenoid transporter was firstly discovered using silkworm yellow-cocoon strains, known as Bombyx mori carotenoid selective transport system (BmCSTS). However, molecular mechanisms underlying the gene/protein expression and regulations in vivo remain elusive. Here, 620 differentially expressed genes (DEGs), 1965 differentially expressed proteins (DEPs), and 67 co-genes (namely Co-DEGs-DEPs) from yellowcocoon silkworm middle silk glands (MSGs) were identified by RNA-Seq based transcriptome and SWATHbased proteomic analysis. Notably, previously identified CBP-alike key factors were further demonstrated significantly up-regulated in all six B. mori yellow-cocoon strains (NB, NB × 306, 306 × NB, NB × 798, 798 × NB, and XH × NB) used in this study. The GO and KEGG analysis results clustered most DEGs and DEPs into multiple biological processes including both cellular components and molecular functions, especially protein synthesis and energy-metabolic pathways. The protein–protein interaction (PPI) network analysis results further revealed a predominant ribosome-biogenesis regulation working system during silkworm yellow-cocoon color formation. Additionally, high-efficient energy production and metabolism pathways (i.e. oxidative phosphorylation and citrate cycle) were also discovered, which might facilitate key gene/protein expressions and functions in BmCSTS, such as CBP and Cameo2.

Association of GSTP1 and RRM1 Polymorphisms with the Response and Toxicity of Gemcitabine-cisplatin Combination Chemotherapy in Chinese Patients with Non-small Cell Lung Cancer

Yuan, Zhi-Jun,Zhou, Wen-Wu,Liu, Wei,Wu, Bai-Ping,Zhao, Jin,Wu, Wei,He, Yi,Yang, Shuo,Su, Jing,Luo, Yi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Previous studies showed that genetic polymorphisms of glutathione S-transferase P1 (GSTP1) were involved in glutathione metabolism and genetic polymorphisms of ribonucleotide reductase (RRM1) were correlated with DNA synthesis. Here we explored the effects of these polymorphisms on the chemosensitivity and clinical outcome in Chinese non-small cell lung cancer (NSCLC) patients treated with gemcitabine-cisplatin regimens. Materials and Methods: DNA sequencing was used to evaluate genetic polymorphisms of GSTP1 Ile105Val and RRM1 C37A-T524C in 47 NSCLC patients treated with gemcitabine-cisplatin regimens. Clinical response was evaluated according to RECIST criteria after 2 cycles of chemotherapy and toxicity was assessed by 1979 WHO criteria (acute and subacute toxicity graduation criteria in chemotherapeutic agents). Results: There was no statistical significance between sensitive and non-sensitive groups regarding the genotype frequency distribution of GSTP1 Ile105Val polymorphism (p>0.05). But for RRM1 C37A-T524C genotype, sensitive group had higher proportion of high effective genotype than non-sensitive group (p=0.009). And according to the joint detection of GSTP1 Ile105Val and RRM1 C37A-T524C polymorphisms, the proportion of type A (A/A + high effective genotype) was significantly higher in sensitive group than in non-sensitive group (p=0.009). Toxicity showed no correlation with the genotypes between two groups (p>0.05). Conclusions: Compared with single detection of genetic polymorphisms of GSTP1 Ile105Val or RRM1 C37A-T524C, joint detection of both may be more helpful for patients with NSCLC to receive gemcitabine-cisplatin regimens as the first-line chemotherapy. Especially, genetic polymorphism of RRM1 is more likely to be used as an important biomarker to predict the response and toxicity of gemcitabine-cisplatin combination chemotherapy in NSCLC.

Fabrication of silica/PVA‑co‑PE nanofiber membrane for oil/water separation

Yuanli Chen,Hui Fan,Xinlin Zha,Wenwen Wang,Yi Wu,Yi Xiong,Kun Yan,Yuedan Wang,Dong Wang 한국의류학회 2021 Fashion and Textiles Vol.8 No.1

High efficiency and anti-pollution oil/water separation membrane has been widely explored and researched. There are a large number of hydroxyl groups on the surface of silica, which has good wettability and can be used for oil-water separation membranes. Hydrophilic silica nanostructures with different morphologies were synthesized by changing templates and contents of trimethylbenzene (TMB). Here, silica nanospheres with radical pores, hollow silica nanospheres and worm-like silica nanotubes were separately sprayed on the PVA-co-PE nanofiber membrane (PM). The abundance of hydroxyl groups and porous structures on PM surfaces enabled the absorption of silica nanospheres through hydrogen bonds. Compared with different silica nanostructures, it was found that the silica/PM exhibited excellent super-hydrophilicity in air and underwater “oil-hating” properties. The PM was mass-produced in our lab through meltextrusion- phase-separation technique. Therefore, the obtained membranes not only have excellent underwater superoleophobicity but also have a low-cost production. The prepared silica/PM composites were used to separate n-hexane/water, silicone oil/ water and peanut oil water mixtures via filtration. As a result, they all exhibited efficient separation of oil/water mixture through gravity-driven filtration.

Albumin-to-Fibrinogen Ratio as an Independent Prognostic Parameter in Untreated Chronic Lymphocytic Leukemia: A Retrospective Study of 191 Cases

Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2

Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.

Voltage Clamping Balance for Charging/Discharging Battery Module

Yi-Yuan Chung,Tsorng-Juu Liang,Wei-Jing Tseng,Jwo-Hann Liu 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

This paper proposes a voltage clamping balance (VCB) method applied with battery power module (BPM) system. The VCB method, which indicates that battery balance is activated after the battery voltage reached its rated value, can save the cost of battery state of charge (SOC) estimation and increases system reliability while keeping the battery voltage in safe operating region. Otherwise, a VCB discharging experimental result shows that the proposed method can release more charge from battery than conventional discharging method in this paper. The proposed system composed of several BPMs and a system control circuit can provide energy as a DC voltage source in discharge mode, and can be charged by an external DC voltage source in charge mode. Meanwhile, the characteristics of BPM system such as high flexibility and capability of hot-swapping can be achieved by the proposed system. Finally, a 24-V LiFePO4 BPM system prototype containing 4 BPMs with 200-W power rating for each BPM is built to verify the feasibility of the proposed VCB method and the proposed BPM system.

Lateral stiffness of corner-supported steel modular frame with splice connection

Yi-Fan Lyu,Guo-Qiang Li,Ke Cao,Si-Yuan Zhai,De-Yang Kong,Xuan-Yi Xue,Heng Li 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.48 No.3

This paper proposes a comprehensive investigation on lateral stiffness of corner-supported steel modular frame using splice connection. A full-scale modular frame with two stacked steel modules under lateral load is tested. Ductile pattern in the transfer of lateral load is found in the final failure mode. Two types of lateral stiffness, including tangent stiffness and secant stiffness, are defined from the load-displacement due to the observed nonlinearity. The difference between these two types of stiffness is found around 20%. The comparisons between the experimental lateral stiffness and the predictions of classical methods are also conducted. The D-value method using hypothesis of independent case is a conservative option for predicting lateral stiffness, which is more recommended than method of contraflexural bending moment. Analyses on two classical shortrod models, including fix-rod model and pin-rod model, are further conducted. Results indicate that fix-rod model is more recommended than pin-rod model to simplify splice connection for simulation on lateral stiffness of modular frame in elastic design stage.