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Huang, Wei-Yi,Chen, Dong-Hui,Ning, Li,Wang, Li-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
The gene encoding the Nin one binding (NOB1) protein which plays an essential role in protein degradation has been investigated for possible tumor promoting functions. The present study was focused on NOB1 as a possible therapeutic target for breast cancer treatment. Lentivirus mediated NOB1 siRNA transfection was used to silence the NOB1 gene in two established breast cancer cell lines, MCF-7 and MDA-MB-231, successful transfection being confirmed by fluorescence imaging. NOB1 deletion caused significant decline in cell proliferation was observed in both cell lines as investigated by MTT assay. Furthermore the number and size of the colonies formed were also significantly reduced in the absence of NOB1. Moreover NOB1 gene knockdown arrested the cell cycle and inhibited cell cycle related protein expression. Collectively these results indicate that NOB1 plays an essential role in breast cancer cell proliferation and its gene expression could be a therapeutic target.
Study on Aging Characteristics and Chemical Composition of Hydrogenated Transformer Oil
Yi-Hua Qian,Yi-Bin Huang,Qiang Fu,Zhen-Sheng Zhong 대한전기학회 2013 Journal of Electrical Engineering & Technology Vol.8 No.3
Under the condition of Baader aging, the chemical composition variation and the influence of transformer oil aging on electrical properties such as dielectric loss factor and physicchemical properties such as interfacial tension were studied in the aging precess. Moreover, the correlation between hydrogenated transformer oil electrical and physic-chemical properties and its chemical composition variation were also investigated. The results show that these parameters of physic-chemical and electrical properties of hydrogenated transformer oil relate to each other and have closed correlation with chemical composition.
Bostrycin production by agro-industrial residues and its potential for food processing
Yi-Hsuan Huang,Wen-Jen Yang,Chih-Yu Cheng,Huang-Mo Sung,Shuen-Fuh Lin 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
Bostrycin, a red antibacterial agent produced by Nigrospora sp. no. 407, is considered for meat processing. To optimize production, the culture conditions of submerged fermentation (SmF) and solid-state fermentation (SSF) were investigated. The optimal SmF conditions were a medium containing 1.0% cane molasses and incubation at 30 C and 150 rpm for 6 days. In SSF, other than bostrycin, less pigment was produced and the optimal ratio of bagasse to water was 1:2 for 10 days. The production and recovery rate of bostrycin by SmF were 120 mg/L and 40%, respectively. Bostrycin exhibited thermostable, pHdependent color change and dose-dependent antibacterial activity against Clostridium botulinum. Bostrycin-modified meat turned strong red for at least 24 h and could not be removed by washing; bostrycin maintained its antibacterial activity with a bacteriostasis rate of 91% on Staphylcoccus aureus. This is an easy and inexpensive means of acquiring bostrycin from molasses and sugarcane.
A Versatile Fruit and Vegetable Image Recognition Method based on Deep Convolutional Neural Networks
( Yi-hsuan Huang ),( Ta-te Lin ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Due to the increasing labor costs and shortage of labor in the agricultural industry, automation in agriculture has become ever more important. This paper proposes a versatile and automatic fruit and vegetable recognition method through the use of computer vision and deep neural networks. The proposed method allows for detection, recognition, and localization of selected fruits and vegetables via images or video streams. Therefore, the method can be used in various applications in agriculture such as robotic harvest, greenhouse management, or crop phenotyping. To detect fruits or vegetables in images, traditional image processing algorithms have some limitations due to occlusions and background variations. Different fruits or vegetables may require different algorithms. However, deep convolutional neural networks have brought about a breakthrough in dealing with this problem. The significance of deep neural networks in imaging processing is that features are no longer extracted by image processing algorithms. Instead, the network will learn by itself from the input data and extract the important features, called deep features. Therefore, we apply deep convolutional neural networks with You Only Look Once (YOLO), a real-time object detection algorithm, to build a versatile image recognition model for selected fruits and vegetables. Using YOLO, the models are trained with five kinds of fruits and vegetables: apple, tomato, cucumber, orange and strawberry. There are two kinds of models developed: ‘one vs. all’ and ‘one vs. one’ models. These models are compared to obtain the ensemble model. In addition, the effects of different phenotype between training data sets and testing data sets are also evaluated. Finally, the optimized model is applied in the recognition system and multiple kinds of fruits are recognized. We also tested the method with images and video streams acquired from greenhouses to evaluate the performance of the method.
Taiwan Neurosurgical Spine Society: The New Shining Star
Yi-Hsuan Kuo,Jau-Ching Wu,Wen-Cheng Huang,Ming-Chao Huang,E-Jian Lee,Henrich Cheng 대한척추신경외과학회 2018 Neurospine Vol.15 No.4
As spine surgery flourished in Taiwan and neurosurgeons became more involved in spine surgery towards the end of the 20th century, the Taiwan Neurosurgical Spine Society (TNSS), earlier named the Taiwan Neurospinal Society, was established on March 11, 2001. As its main founder, Dr. Chun-I Huang was elected as the first president of the TNSS. The goals of the TNSS were to promote research, to hold academic seminars, to participate in international conferences, and to exchange clinical experiences. The mission of the TNSS was successful, and the profession of spine surgery in Taiwan advanced during the first decade of the 21st century, culminating in the TNSS joining ASIA SPINE in 2010. Since its establishment, the TNSS has always been supportive of collaboration and communication with the Korean Spinal Neurosurgery Society and the Neurospinal Society of Japan. Through periodical meetings, supported by the TNSS, surgeons worldwide have enjoyed a platform of sharing and mutual learning. To further promote academic research, the TNSS has officially supported the journal Neurospine since 2018. With extensive efforts from local and international surgeons, the TNSS will continue to adhere to its mission and to advance the profession of spine surgery.
Huang Jiang,Yi Quanyong,You Yuhong,Chen Yao,Niu Tongtong,Yi Li,Zhang Ji,Ji Xiaoyan,Xu Guoxu,Zou Weijie,Ji Fangfang,Luo Weifeng 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3
Background The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance. Objective The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry. Results We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin. Conclusion We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR. Background The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance. Objective The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry. Results We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin. Conclusion We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR.
Huang, Chang-Yi,Nixon, Peter F.,Duggleby, Ronald G. Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.1
Pyruvate decarboxylase (PDC) catalyzes the conversion of pyruvate to acetaldehyde as the penultimate step in alcohol fermentation. The enzyme requires two cofactors, thiamin diphosphate (ThDP) and $Mg^{2+}$, for activity. Zymomonas mobilis PDC shows a strong preference for pyruvate although it will use the higher homologues 2-ketobutyrate and 2-ketovalerate to some extent. We have investigated the effect of mutagenesis of valine 111 and leucine 112 on the substrate specificity. V111 was replaced by glycine, alanine, leucine, and isoleucine while L112 was replaced by alanine, valine, and isoleucine. With the exception of L112I, all mutants retain activity towards pyruvate with $k_{cat}$ values ranging from 40% to 139% of wild-type. All mutants show changes from wild-type in the affinity for ThDP, and several (V111A, L112A, and L112V) show decreases in the affinity for $Mg^{2+}$. Two of the mutants, V111G and V111A, show an increase in the $K_m$ for pyruvate. The activity of each mutant towards 2-ketobutyrate and 2-ketovalerate was investigated and some changes from wild-type were found. For the V111 mutants, the most notable of these is a 3.7-fold increase in the ability to use 2-ketovalerate. However, the largest effect is observed for the L112V mutation which increases the ability to use both 2-ketobutyrate (4.3-fold) and 2-ketovalerate (5.7-fold). The results suggest that L112 and, to a lesser extent, V111 are close to the active site and may interact with the alkyl side-chain of the substrate.