Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

豪雨時 浮遊砂의 最適 SAMPLING 間隔에 關한 硏究(渭川 流域을 中心으로)

趙廷錫,韓良洙,朴相圭 대구대학교 과학기술연구소 1996 科學技術硏究 Vol.3 No.1

This study calculated the optimal sampling interval of suspended sediment on storm in terms of Kalman filter method after collected data the discharge and suspended sediment of Museong place, the downstream of the Wi stream basin that is one of the IHP representative basin the Nakdong river water systems. We'll have to study more specifically about the various elements closely related to the discharge with the reasonable and correct standard of the error variance in the future and we finds out the suspended sediment optimal sampling intervals in other basins it will be applied by the characteristic of them.

객체 관계형 데이타베이스 관리 시스템을 이용한 디지털 앨범의 설계 및 구현

이준서,장염승,한기준 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

As the storage capacity of a computer system is rapidly increasing and the cost of computer hardware is decreasing continuously, users can easily manage multimedia data on the personal computer. Especially, various image view programs are widely used to manage image files at the present time. However, since most of these programs do not use a database management system(DBMS), it is very difficult for users to use them efficiently. In this paper, we designed and implemented a Digital Album by using an object-relational database management system, called PARODY(Persistent, Almost, Relational Object Database sYstem) that implements a persistent object database with a relatively simple interface to its C++ class library. Since PARODY is used as a data storage manager of the Digital Album, users can manage(i.e., insert, update, and delete) an image database and search for the required image data from the image database easily. In addition, we use OWL(Object Windows Library) of Borland C++ to implement the user interface of the Digital Album efficiently.

Differences in Epidermal Growth Factor Receptor Gene Mutations and Relationship with Clinicopathological Features in NSCLC Between Uygur and Han Ethnic Groups

Zhang, Yan,Wang, Qiang,Han, Zhi-Gang,Shan, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To investigate differences in mutations of epidermal growth factor receptor (EGFR) gene and relationships with clinicopathological features in patients with non-small cell lung cancer (NSCLC) between Uygur and Han ethnic groups. Methods: The Scorpions amplification refractory mutation system (Scorpions ARMS) was used to measure mutations in exons 18, 19, 20 and 21 of the EGFR gene in paraffin-embedded tumor tissue from NSCLC cases, and statistical analysis was performed to investigate links with clinicopathological features in different histological types of NSCLC. Results: Results from ARMS testing showed EGFR mutations in tumor tissues from six (6) of 50 NSCLC patients of Uygur ethnic group, with a positive rate of 12.0%; four of them (4) had exon 19 deletion in EGFR, and two (2) had L858R point mutation in exon 21 of EGFR. Statistically significant difference was noted in EGFR genetic mutation between adenocarcinoma and non-adenocarcinoma (P < 0.05), but no differences with gender, age group, smoking status, or stage (P > 0.05). EGFR mutations were detected in tumor tissues from 27 of 49 NSCLC patients of Han ethnic group, with a positive rate of 55.1%; 19 of them had exon 19 deletions, seven (7) had L858R point mutations in exon 21 of EGFR and one (1) had mutations in both exon 18 G719X and exon 20 T790M of EGFR. Statistically significant differences were noted in EGFR genetic mutations between genders and between adenocarcinoma and non-adenocarcinoma (P<0.05), but not with age group, smoking status, or stage (P > 0.05). Conclusion: Statistically significant differences were noted in the positive rates of EGFR genetic mutations in NSCLC patients between Uygur and Han ethnic groups, with lower positive rates for the Uygur cases.

Genetic Variants at 6p21.1 and 7p15.3 Identified by GWASs of Multiple Cancers and Ovarian Cancer Risk: a Case-control Study in Han Chinese Women

Li, Da-Ke,Han, Jing,Liu, Ji-Bin,Jin, Guang-Fu,Qu, Jun-Wei,Zhu, Meng,Wang, Yan-Ru,Jiang, Jie,Ma, Hong-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

A recent study summarized several published genome-wide association studies (GWASs) of cancer and reported two pleiotropic loci at 6p21.1 and 7p15.3 contributing to multiple cancers including lung cancer, noncardia gastric cancer (NCGC), and esophageal squamous-cell carcinoma (ESCC) in Han Chinese. However, it is not known whether such genetic variants have similar effects on the risk of gynecologic cancers, such as ovarian cancer. Hence, we explored associations between genetic variants in 6p21.1 and 7p15.3 and ovarian cancer risk in Han Chinese women. We performed an independent case-control study by genotyping the two loci (rs2494938 A > G at 6p21.1 and rs2285947 A > G at 7p15.3) in a total of 377 ovarian cancer cases and 1,034 cancer-free controls using TaqMan allelic discrimination assay. We found that rs2285947 at 7p15.3 was significantly associated with risk of ovarian cancer with per allele odds ratio (OR) of 1.33 [95% confidence interval (CI): 1.08-1.64, P=0.008]. However, no significant association was observed between rs2494938 and ovarian cancer risk. Our results showed that rs2285947 at 7p15.3 may also contribute to the development of ovarian cancer in Han Chinese women, further suggesting pleiotropy of 7p15.3 in multiple cancers.

Identification of Bovine Pregnancy-Specific Whey Proteins using Two-Dimensional Gel Electrophoresis

Han, Rong-Xun,Choi, Su-Min,Kim, Myung-Youn,Quan, Yan Shi,Kim, Baek-Chul,Diao, Yun Fei,Koqani, Reza,Park, Chang-Sik,Jin, Dong-Il The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.4

The early diagnosis of bovine pregnancy is an essential component of successful reproductive planning on farms, because lack of bovine pregnancy over the long term results in reproductive failure and low milk yield-the latter of which is a special concern on dairy farms. This study was designed to identify early pregnancy-specific whey proteins in bovine, by comparing milk samples collected from cattle during pregnancy (Days 30 and 50) and from non-pregnant cattle. In this study, differentially expressed proteins in five pregnant and five non-pregnant Holstein dairy cattle were investigated and compared, using proteomics analysis. The first dimension was applied to a pH $3.0{\sim}10.0$ strip, by loading a 2-mg milk protein sample. After the second-dimension separation was performed, the gels were stained with colloidal Coomassie brilliant blue. The stained gels were scanned and the images were analyzed, to detect variations in protein spots between non-pregnant and pregnant cattle milk protein spots, using ImageMaster, this was followed by analysis with MALDI TOF-MS. Analysis of the 2-DE gel image resulted in a total of approximately $500{\sim}600$ protein spots, of which 12 spots were differentially expressed, six spots were up-regulated, and four spots were down-regulated; two spots were identified as pregnancy-specific proteins. These proteins were identified as lactoferrin, NA-DH dehydrogenase subunit 2, albumin, serum albumin precursor and transferrin. Our results via 2-D PAGE analysis revealed composite profiles of several milk proteins related to early bovine pregnancy, implying the possible use of these milk proteins in the early detection of bovine pregnancy.

Experimental and numerical studies of aerodynamic forces on vehicles and bridges

Yan Han,C.S. Cai,Jiexuan Hu,Zhengqing Chen,Chunguang Li 한국풍공학회 2013 한국풍공학회지 Vol.17 No.2

An accurate identification of the aerodynamic characteristics of vehicles and the bridge is the premise for the coupled vibration analysis of a wind-vehicle-bridge system. At present, the interaction of aerodynamic forces between the road vehicles and bridge is ignored in most previous studies. In the present study, an experimental setup was developed to measure the aerodynamic characteristics of vehicles and the bridge for different cases in a wind tunnel considering the aerodynamic interference. The influence of the wind turbulence, the wind speed, the vehicle interference, and the vehicle position on the aerodynamic coefficients of vehicles, and the influence of vehicles on the static coefficients of the bridge were investigated, based on the experimental results. The variations in the aerodynamic characteristics of vehicles and the bridge were studied and the measured results were validated according to the results of surface pressure measurements on the vehicle and the bridge. The measured results were further validated by comparing the measured results with values derived numerically. The measured results showed that the wind turbulence, the vehicle interference, and the vehicle position significantly affected the aerodynamic coefficients of vehicles. However, the influence of the wind speed on the aerodynamic coefficients of the studied vehicle is small. The static coefficients of the bridge were also significantly influenced by the presence of vehicles.

Appraise and analysis of dynamical stability of cable-driven lower limb rehabilitation training robot

Yan-lin Wang,Ke-yi Wang,Wan-li Wang,Peng-cheng Yin,Zhuang Han 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.11

This paper presents a problem that traditional methods cannot evaluate the dynamical stability of the under-constraint cable-driven lower limb rehabilitation training robot (CLLRTR). An analytical method is presented to evaluate the dynamical stability of CLLRTR. Firstly, position performance factor, posture performance factor and cable tension performance factor are defined based on the kinematics and dynamics of CLLRTR. An appraisal index and method of the dynamical stability for CLLRTR with the hybrid force-position-pose approach is proposed by using the weighted average method among three performance factors. Secondly, the stable workspace and robustness workspace with the external forces are defined according to the stability margin. Finally, the simulation analysis and the experimental study are used to illustrate the distribution of the dynamical stability in the whole workspace of CLLRTR. The results show that the experimental results are the same as the theoretical simulation analysis results. So the appraisal index of the dynamical stability can be used to evaluate the dynamical stability of CLLRTR. It will provide a foundation for the trajectory planning and control strategy of CLLRTR training pattern.

Synthesis and Viscoelastic Behavior of Non-Stoichiometric Spinel Ferrite Particle Suspension

Han, Jae Kyeong,Han, Wen Jiao,Gao, Chun Yan,Dong, Yu Zhen,Choi, Hyoung Jin IEEE 2018 IEEE transactions on magnetics Vol.54 No.11

<P>Magnetorheological (MR) fluid with tunable and reversible phase transition is one of the most promising smart materials for engineering applications. In this paper, to improve the MR performance of ferrites, non-stoichiometric Zn-doped spinel ferrite (Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB>) nanoparticles with a high saturation magnetization and a truncated octahedron-like shape were synthesized using a thermal decomposition process. The crystallographic structure was analyzed using X-ray diffraction and field emission transmission electron microscope image. The Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB> had a high saturation magnetization value of 101 <TEX>$\text {A}\cdot \text {m}^{2}$</TEX>/kg at 933 kA/m, showing higher saturation magnetization than other ferrites. The MR characteristics of the Zn<SUB>0.417</SUB>Fe<SUB>2.583</SUB>O<SUB>4</SUB> particles suspended in silicone oil were studied using a rotation rheometer under various magnetic field strengths. This MR fluid exhibited typical MR characteristics, and its flow curves were fit well with the Herschel–Bulkley model.</P>

The influence of vehicles on the flutter stability of a long-span suspension bridge

Yan Han,Shuqian Liu,C.S. Cai,Jianren Zhang,Suren Chen,Xuhui He 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.20 No.2

The presence of traffic on a slender long-span bridge deck will modify the cross-section profileof the bridge, which may influence the flutter derivatives and in turn, the critical flutter wind velocity of thebridge. Studies on the influence of vehicles on the flutter derivatives and the critical flutter wind velocity ofbridges are rather rare as compared to the investigations on the coupled buffeting vibration of thewind-vehicle-bridge system. A typical streamlined cross-section for long-span bridges is adopted for bothexperimental and analytical studies. The scaled bridge section model with vehicle models distributed on thebridge deck considering different traffic flow scenarios has been tested in the wind tunnel. The flutterderivatives of the modified bridge cross section have been identified using forced vibration method and theresults suggest that the influence of vehicles on the flutter derivatives of the typical streamlined cross-sectioncannot be ignored. Based on the identified flutter derivatives, the influence of vehicles on the flutter stabilityof the bridge is investigated. The results show that the effect of vehicles on the flutter wind velocity isobvious.