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Yanhui Lu,Xu-Song Zheng,Qi Liang,Hong-Xing Xu,Yajun Yang,Junce Tian,Xiaochan He,Zhong-Xian Lu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4
The pink stemborer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae), has gradually become amajor threat to rice production in Asian rice growth area. Using reverse-transcription quantitative real-time PCR (qRT-PCR) to investigate gene expression of S. inferens under different conditions is a popular technique. However, numerous studies demonstrate that the experimental conditions affect the stability of reference genes. Thus, it is necessary to normalize qRT-PCR data under a specific condition using a stable reference gene as an internal control. In the present study, five algorithms (Delta Ctmethod, Bestkeeper, geNorm,Normfinder, and RefFinder) and seven candidate reference genes were evaluated to find out the most suitable reference genes under different biotic and abiotic conditions including different tissues and developmental stages, exposure to insecticides, different rice varieties, and different diets. Our results showed that the more stable series of reference genes were as follows: GAPDH, RPL10, and RPS3 for different tissues;GAPDH and RPS3 for developmental stages; RPS3 and EF1 for larval insecticide exposure; RPS3 and RPL10 for larvae reared on different rice varieties; RPS3 and ACTB for larvae fed on different diets. Hence, a combination of the best sets of reference genes for normalization of expression analysis will increase the accuracy and reliability of qRT-PCR data in S. inferens. In addition, these results establish the basis for selection of reference genes in other lepidopterous insect species.
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Jun Zhang,Yanhui Guo,Weiwei Lu,Xuming Guo,Mingchen Xu,Hongbo Guo 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.5
Highly dispersive ammonium dimolybdate crystals with high purity have been prepared through reaction–evaporation–crystallization united route using commercial molybdenum oxide as starting material. The as-prepared ammonium dimolybdate uniform crystals were systematically characterized by X-ray powder diffraction, transmission-reflection optical microscope, Fourier transform infrared spectroscopy and TG-DSC thermal analysis. The Fsss grain diameter, loose packed density, and crystal-grain size distribution were also measured by the Malvern laser particle sizer. These collective characterization and analysis reveal that the ammonium dimolybdate grains are non-agglomerated uniform crystals with well normal distribution and geometric shape. The average Fsss diameter, d(0.5) and loose packed density are 433.316 mm, 470.981 mm and 1.592 g cm3, respectively, which could well meet the quality requirements for the subsequent production of molybdenum powders.
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( Huijin Cao ),( Yanhui Lu ),( Jun Cai ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.9
In this paper, we address the problem of linear minimum mean-squared error (MMSE) transmitter design for the cognitive radio (CR) multi-user multiple-input single-output (MU-MISO) broadcasting channel (BC), where the cognitive users are subject to not only a sum power constraint, but also a interference power constraint. Evidently, this multi-constraint problem renders it difficult to solve. To overcome this difficulty, we firstly transform it into its equivalent formulation with a single constraint. Then by utilizing BC-MAC duality, the problem of BC transmitter design can be solved by focusing on a dual MAC problem, which is easier to deal with due to its convexity property. Finally we propose an efficient two-level iterative algorithm to search the optimal solution. Our simulation results are provided to corroborate the effectiveness of the proposed algorithm and show that this proposed CR MMSE-based scheme achieves a suboptimal sum-rate performance compared to the optimal DPC-based algorithm with less computational complexity.
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( Bing Ning ),( Shouyi Yang ),( Xiaomin Mu ),( Yanhui Lu ),( Wanming Hao ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.11
The energy-efficient design of sensing-based spectrum sharing of a multi-input and multi-output (MIMO) cognitive radio (CR) system with imperfect multiple antenna spectrum sensing is investigated in this study. Optimal resource allocation strategies, including sensing time and power allocation schemes, are studied to maximize the energy efficiency (EE) of the secondary base station under the transmit power and interference power constraints. EE problem is formulated as a nonlinear stochastic fractional programming of a nonconvex optimal problem. The EE problem is transformed into its equivalent nonlinear parametric programming and solved by one-dimension search algorithm. To reduce searching complexity, the search range was founded by demonstration. Furthermore, simulation results confirms that an optimal sensing time exists to maximize EE, and shows that EE is affected by the spectrum detection factors and corresponding constraints.
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( Xue Cai ),( Dan Luo ),( Shuling Liu ),( Ruxue Li ),( Yanhui Lu ),( Mingzi Li ),( Shanhu Qiu ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1
Background/Aims: Older adults are vulnerable to central obesity, while the association of changes in central fatness with risk of diabetes and metabolic control has not been investigated among this particular population. This study was aimed to address these issues. Methods: A total of 1,815 adults aged ≥ 60 years without diabetes at baseline were followed for 4 years. Incident diabetes was ascertained based on plasma glucose, hemoglobin A1c, medical history, and/or the use of anti-diabetic drugs. Central fatness was assessed by waist circumference (WC), waist-height ratio (WHtR), and body roundness index (BRI). Logistic re gression analyses were used to assess the association of changes in central fatness with risk of diabetes, along with dose-re sponse and mediation analyses. Results: During the 4-year follow-up, 177 participants developed diabetes. The risk of diabetes was increased by 42%, 41%, and 40% per 1 standard deviation increases in WC, WHtR, and BRI, respectively, in multivariable-adjusted models (all p < 0.01). Moreover, these relationships were all linearly-shaped (all p<sub>nonlinearity</sub> ≥ 0.11). Increases in WC, WHtR, and BRI correlat ed with increases in hemoglobin A1c, triglycerides-and-glucose index, triglycerides, white blood cell, and C-reactive protein (all p ≤ 0.04). Yet only changes in hemoglobin A1c and triglycerides-and-glucose index were identified as the possible mediators for risk of diabetes, with their mediating effect being about 35% and 21%, respectively. Conclusions: Increases in central fatness were related to elevated risk of diabetes, and this association might be partly ex plained by the worsening of glycemic control and insulin resistance in older adults.
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